Organism : Desulfovibrio vulgaris Hildenborough | Module List:
Module 25 Profile

GeneModule member RegulatorRegulator MotifMotif
Network Help

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges.

Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif
Regulators for Module 25

There are 9 regulatory influences for Module 25

Regulator Table (9)
Regulator Name Type
DVU2567 tf
DVU1754
DVU2319		 combiner
DVU0629 tf
DVU1754 tf
DVU0653
DVU2251		 combiner
DVU0936 tf
DVU2909
DVU3142		 combiner
DVU2114
DVU0569		 combiner
DVU2557
DVU2251		 combiner

Regulator Help

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type.

tf: Transcription factor

ef: Environmental factor

combiner: Combinatorial influence of a tf or an ef through logic gate. Table is sortable by clicking on the arrows next to column headers.

Motif information (de novo identified motifs for modules)

There are 2 motifs predicted.
Click on the RegPredict links to explore the motif in RegPredict.

Motif Table (2)
Motif Id e-value Consensus Motif Logo RegPredict
49 1.50e+00 CA.cCGcCAtc
Loader icon
 RegPredict
50 6.00e+04 AGTTTTT
Loader icon
 RegPredict
Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment

Regulon 25 is enriched for following functions.

KEGG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini Hochberg pvalue Genes with function Method
Metabolic pathways kegg pathway 1.98e-02 2.99e-02 6/23
Nucleotide Metabolism kegg subcategory 2.13e-03 1.22e-02 3/23
Replication and Repair kegg subcategory 0.00e+00 3.00e-06 6/23
Metabolism kegg category 2.58e-03 1.79e-02 11/23
Genetic Information Processing kegg category 1.43e-04 1.78e-03 6/23
Metabolism kegg category 1.45e-02 3.36e-02 7/23
Genetic Information Processing kegg category 2.15e-02 4.45e-02 3/23
Replication and Repair kegg subcategory 1.01e-04 1.66e-03 3/23
Global kegg category 2.25e-02 4.61e-02 6/23
Metabolism kegg subcategory 2.25e-02 4.61e-02 6/23
Metabolic pathways kegg pathway 1.98e-02 4.22e-02 6/23

GO Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
DNA recombination biological_process 1.70e-05 1.32e-04 3/23
DNA binding molecular_function 7.44e-04 1.56e-03 4/23
DNA-directed DNA polymerase activity molecular_function 0.00e+00 0.00e+00 14/23

COG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Replication, recombination and repair cog subcategory 3.10e-05 1.29e-03 5/23
Information storage and processing cog category 1.17e-03 2.20e-02 7/23
Information storage and processing cog category 9.85e-04 1.93e-03 7/23
Replication, recombination and repair cog subcategory 3.10e-05 9.70e-05 5/23
Functions Help

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Members for Module 25

There are 23 genes in Module 25

Gene Member Table (23)
Name Common name Type Gene ID Chromosome Start End Strand Description TF
DVU0144 CDS 2796577 chromosome 179041 179643 + cytidyltransferase-like protein False
DVU0377 trxB-1 CDS 2794635 chromosome 427507 428424 - thioredoxin reductase False
DVU0496 polA CDS 2794724 chromosome 565280 568327 - DNA polymerase I False
DVU0735 CDS 2794949 chromosome 822186 822620 + MOSC domain-containing protein False
DVU0816 cobQ CDS 2795019 chromosome 904464 906095 + cobyric acid synthase False
DVU1224 nfo CDS 2794119 chromosome 1315670 1316518 + endonuclease IV False
DVU1253 CDS 2794599 chromosome 1340271 1340399 - hypothetical protein DVU1253 False
DVU1527 CDS 2793944 chromosome 1594709 1595902 - phage integrase family site specific recombinase False
DVU1763 CDS 2795810 chromosome 1826856 1827011 - hypothetical protein DVU1763 False
DVU1804 CDS 2796713 chromosome 1866458 1868287 - glycosyl transferase group 1 family protein False
DVU1899 CDS 2794710 chromosome 1973502 1974257 - DNA repair protein RecO False
DVU1983 CDS 2793789 chromosome 2060785 2062008 + hypothetical protein DVU1983 False
DVU2111 CDS 2793383 chromosome 2206454 2207356 + LysR family transcriptional regulator True
DVU2197 CDS 2793660 chromosome 2292764 2293693 - phage integrase family site specific recombinase False
DVU2272 CDS 2795228 chromosome 2361044 2363497 - formate acetyltransferase False
DVU2350 gid CDS 2795524 chromosome 2445526 2446950 + tRNA (uracil-5-)-methyltransferase Gid False
DVU2760 CDS 2794033 chromosome 2868372 2868983 + hypothetical protein DVU2760 False
DVU3114 kdsB CDS 2796606 chromosome 3263483 3264241 - 3-deoxy-manno-octulosonate cytidylyltransferase False
DVU3170 cobJ CDS 2795875 chromosome 3326787 3327641 + precorrin-3b C17-methyltransferase False
DVU3317 CDS 2796053 chromosome 3494848 3495018 - hypothetical protein DVU3317 False
DVU3377 dgkA CDS 2795691 chromosome 3550058 3550432 - diacylglycerol kinase False
DVU3394 CDS 2796413 chromosome 3569082 3569261 + hypothetical protein DVU3394 False
DVUA0106 CDS 2781592 pDV 143522 144646 - YopN family type III secretion target protein False

Genes Help

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Help

What is a module?

Regulatory units (modules) in the Network Portal are based on the network inference algorithm used. For the current version, modules are based on cMonkey modules and Inferelator regulatory influences on these modules. More specifically, module refers to set of genes that are conditionally co-regulated under subset of the conditions. Identification of modules integrates co-expression, de-novo motif identification, and other functional associations such as operon information and protein-protein interactions.

Module Overview

The landing module page shows quick summary info including co-expression profiles, de-novo identified motifs, and transcription factors and/or environmental factors as regulatory influences. It also includes module residual, motif e-values, conditions and links to other resources such as NCBI and Microbesonline. . If a transcription factor is included in the manually curated RegPrecise database, further information from RegPrecise is shown, allowing users to perform comparative analysis.

Expression Profiles

Expression profiles is a plot of the expression ratios (log10) of the module's genes, over all subset of the conditions included in the module. The X-axis represent conditions and the Y-axis represents log10 expression ratios. Each gene is plotted as line plot with different colors. Colored legend for the lines are presented under the plot. This plot is dynamic. Clicking on the gene names in the legend will show/hide the plot for that particular gene. A tooltip will show expression ratio information if you mouseover the lines in the plot.

Motif Locations

Location of the Identified motifs for the module in the upstream regions of the member genes are shown under the expression profiles plot. This plot shows the diagram of the upstream positions of the motifs, colored red and green for motifs #1, and 2, respectively. Intensity of the color is proportional to the significance of the occurence of that motif at a given location. Motifs on the forward and reverse strand are represented over and under the line respectively.

Network

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges. Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif

Regulators

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type. tf: Transcription factor, ef: Environmental factor and combiner:Combinatorial influence of a tf or an ef through logic gate. Tabel is sortable by clicking on the arrows next to column headers.

Motifs

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Functions

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Genes

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Social

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Definitions

Residual: is a measure of bicluster quality. Mean bicluster residual is smaller when the expression profile of the genes in the module is "tighter". So smaller residuals are usually indicative of better bicluster quality.

Expression Profile: is a preview of the expression profiles of all the genes under subset of conditions included in the module. Tighter expression profiles are usually indicative of better bicluster quality.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Genes: Number of genes included in the module.

Functions: We identify functional enrichment of each module by camparing to different functional categories such as KEGG, COG, GO etc. by using hypergeometric function. If the module is significantly enriched for any of the functions, this column will list few of the these functions as an overview. Full list of functions is available upon visiting the module page under the Functions tab.