GO:0030697

S-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity

Details: 
Catalysis of the reaction: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing thymine at position U54 of a transfer RNA. This occurs in most Gram-negative bacteria, some archae, and eukaryotes. [GOC:hjd, ISBN:1555811337]
Evidence Code: 
IEA