Rv3490 Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3490 otsA Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15) CDS 3908236 3909738 + 1 503 500 FALSE

Rv3490 (Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15)) is predicted to be co-regulated in modules bicluster_0162 with residual 0.54 and bicluster_0340 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 45.00 and 5,400.00 for bicluster_0162 and 1,100.00 and 2,300.00 for bicluster_0340 respectively.

These modules are enriched for following go terms: cysteine biosynthetic process from serin..., cysteine biosynthetic process, cysteine metabolic process, L-serine metabolic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Trehalose-phosphate synthase alpha,alpha-trehalose-phosphate synthase
Operon # Operon
2283 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Starch and sucrose metabolism

20
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610626 NP_218007.1 Run
GO:0003825

alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity

alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity

Details: 
Catalysis of the reaction: UDP-glucose + D-glucose-6-phosphate = UDP + alpha,alpha-trehalose-6-phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0003825

alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity

alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity

Details: 
Catalysis of the reaction: UDP-glucose + D-glucose-6-phosphate = UDP + alpha,alpha-trehalose-6-phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0030145

manganese ion binding

manganese ion binding

Details: 
Interacting selectively and non-covalently with manganese (Mn) ions.
GO Category: 
molecular_function
31
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0047260

alpha,alpha-trehalose-phosphate synthase (GDP-forming) activity

alpha,alpha-trehalose-phosphate synthase (GDP-forming) activity

Details: 
Catalysis of the reaction: GDP-D-glucose + glucose-6-phosphate = alpha,alpha-trehalose 6-phosphate + GDP.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0051289

protein homotetramerization

protein homotetramerization

Details: 
The formation of a protein homotetramer, a macromolecular structure consisting of four noncovalently associated identical subunits.
GO Category: 
biological_process
17
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.840000 2.62

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: