Rv3838c Prephenate dehydratase (EC 4.2.1.51)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3838c pheA Prephenate dehydratase (EC 4.2.1.51) CDS 4311704 4312669 - 966 321 FALSE

Rv3838c (Prephenate dehydratase (EC 4.2.1.51)) is predicted to be co-regulated in modules bicluster_0042 with residual 0.58 and bicluster_0267 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 53.00 for bicluster_0042 and 0.00 and 0.05 for bicluster_0267 respectively.

These modules are enriched for following go terms: carboxylic acid biosynthetic process, organic acid biosynthetic process, histidine biosynthetic process, histidine metabolic process, imidazole-containing compound metabolic ..., cellular amino acid metabolic process, small molecule biosynthetic process, single-organism biosynthetic process, aromatic amino acid family biosynthetic ....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
POSSIBLE PREPHENATE DEHYDRATASE PHEA prephenate dehydratase
Operon # Operon
2511 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Prephenate dehydratase Phenylalanine, tyrosine and tryptophan biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Phenylalanine, tyrosine and tryptophan biosynthesis

18
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610974 NP_218355.1 Run
GO:0004664

prephenate dehydratase activity

prephenate dehydratase activity

Details: 
Catalysis of the reaction: prephenate = phenylpyruvate + H2O + CO2.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0004664

prephenate dehydratase activity

prephenate dehydratase activity

Details: 
Catalysis of the reaction: prephenate = phenylpyruvate + H2O + CO2.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0016597

amino acid binding

amino acid binding

Details: 
Interacting selectively and non-covalently with an amino acid, organic acids containing one or more amino substituents.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0033585

L-phenylalanine biosynthetic process from chorismate via phenylpyruvate

L-phenylalanine biosynthetic process from chorismate via phenylpyruvate

Details: 
The chemical reactions and pathways resulting in the formation of L-phenylalanine from other compounds, including chorismate, via the intermediate phenylpyruvate.
GO Category: 
biological_process
1
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0051289

protein homotetramerization

protein homotetramerization

Details: 
The formation of a protein homotetramer, a macromolecular structure consisting of four noncovalently associated identical subunits.
GO Category: 
biological_process
17
Total items in this category:  
GO:0004106

chorismate mutase activity

chorismate mutase activity

Details: 
Catalysis of the reaction: chorismate = prephenate.
GO Category: 
molecular_function
4
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: