Genes

Gene annotations were collated from multiple sources;

Gene essentiality is based on TnSeq Data from: Dembek M, Barquist L, Boinett CJ, et al. High-throughput analysis of gene essentiality and sporulation in Clostridium difficile. mBio. 2015;6(2):e02383. Published 2015 Feb 24.doi:10.1128/mBio.02383-14

Displaying 1 - 50 of 4326
e.g CD630_20320
Title Short Name Product Function Essential? Expression
CD630_00010 dnaA Chromosomal replication initiator protein Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.
CD630_00020 dnaN DNA polymerase III subunit beta Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.
CD630_00030 Putative RNA-binding mediating protein
CD630_00040 recF DNA replication and repair protein RecF The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.
CD630_00050 gyrB DNA gyrase subunit B A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.
CD630_00060 gyrA DNA gyrase subunit A A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.
CD630_00070 Conserved hypothetical protein
CD630_00080 Conserved hypothetical protein
CD630_00090 rsbV Anti-anti-sigma factor
CD630_00100 rsbW Serine-protein kinase RsbW
CD630_00110 sigB RNA polymerase sigma-B factor
CD630_00120 Putative small-molecule-binding protein
CD630_00130 Putative mechanosensitive ion channel protein
CD630_00140 | serS serS1 Seryl-tRNA synthetase 1 Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). Yes
CD630_00150 tadA tRNA specific adenosine deaminase Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2).
CD630_00160 dnaX DNA polymerase III subunits gamma and tau DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. Yes
CD630_00170 DNA binding protein Binds to DNA and alters its conformation. May be involved in regulation of gene expression, nucleoid organization and DNA protection.
CD630_00180 recR DNA repair and recombination protein May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO.
CD630_00181 Conserved hypothetical protein
CD630_00190 Putative glycosyl transferase
CD630_00200 Conserved hypothetical protein
CD630_00210 pycA Pyruvate carboxylase Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
CD630_00220 fusA1 Elongation factor G (EF-G)
CD630_00230 ctsR Transcriptional regulator, CtsR family
CD630_00240 mcsA Activator of protein kinase McsB
CD630_00250 mcsB Tyrosine kinase protein Catalyzes the specific phosphorylation of arginine residues in proteins.
CD630_00260 clpC class III stress response-related ATPase, AAA+superfamily
CD630_00270 radA DNA repair protein DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function.
CD630_00280 disA DNA integrity scanning protein disA Participates in a DNA-damage check-point that is active prior to asymmetric division when DNA is damaged. DisA forms globular foci that rapidly scan along the chromosomes during sporulation, searching for lesions. When a lesion is present, DisA pauses at the lesion site. This triggers a cellular response that culminates in a temporary block in sporulation initiation.
CD630_00290 Putative membrane protein
CD630_00300 Putative membrane protein Yes
CD630_00310 Transcriptional regulator, AraC family
CD630_00320 Putative beta-glucosidase
CD630_00330 Putative glycoside hydrolase
CD630_00340 Putative xylose transporter, sodium:galactosidesymporter family
CD630_00350 Transcriptional regulator
CD630_00360 acoA Acetoin dehydrogenase E1 component (TPP-dependentalpha subunit)
CD630_00370 acoB Acetoin dehydrogenase E1 component (TPP-dependentbeta subunit)
CD630_00380 acoC Acetoin dehydrogenase E2 component(dihydrolipoamide acetyltransferase)
CD630_00390 acoL Acetoin dehydrogenase E3 component(dihydrolipoamide dehydrogenase)
CD630_00400 Transcription antiterminator, PTS operonregulator
CD630_00410 PTS system, galactitol-specific IIA component
CD630_00420 PTS system, galactitol-specific IIB component
CD630_00430 PTS system, galactitol-specific IIC component
CD630_00440 Conserved hypothetical protein
CD630_00450 Putative sugar-phosphate aldolase
CD630_00460 Putative lipoate-protein ligase
CD630_00470 ispD 2-C-methyl-D-erythritol 4-phosphatecytidylyltransferase Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP). Yes
CD630_00480 ispF 2-C-methyl-D-erythritol 2,4-cyclodiphosphatesynthase Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP). Yes
CD630_00490 proS Prolyl-tRNA synthetase Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS. Yes