BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,786,540	Δ1 bp	100%	coding (674/819 nt)	RCAP_rcc01648 →	ABC transporter permease

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,786,540	0	C→.	100.0%	35.4	217	coding (674/819 nt)	RCAP_rcc01648	ABC transporter permease
Reads supporting (aligned to +/- strand): new base (87/117): ref base (4/9): total (91/126)
Fisher's exact test strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.98e-03

Only 100 of 222 total aligned reads displayed.


AGCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.G.GCCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCT  >  NC_014034/1786441‑1786636


   tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGGCTtt                                                                                               <  1:2586514/101‑1 (MQ=255)


   tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGGCTtt                                                                                               <  1:1452474/101‑1 (MQ=255)


   tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGGCTtt                                                                                               >  2:2554746/1‑101 (MQ=255)


    gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGGCTttc                                                                                              <  1:796166/101‑2 (MQ=255)


    gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGGCTttc                                                                                              <  2:227058/101‑2 (MQ=255)


    gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGGCTttc                                                                                              <  1:1983777/101‑2 (MQ=255)


    gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGGCGAATTTCTGG.GCCGATCGAACGGGATCGGCTttc                                                                                              <  2:2308356/101‑2 (MQ=255)


     aTGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCg                                                                                            >  2:4378216/1‑101 (MQ=255)


     aTGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCg                                                                                            <  2:2820813/101‑1 (MQ=255)


     aTGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCg                                                                                            <  1:393980/101‑1 (MQ=255)


      tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGt                                                                                           <  2:3243508/101‑1 (MQ=255)


      tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGt                                                                                           <  1:875822/101‑1 (MQ=255)


        ccGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTAt                                                                                         <  1:3099162/101‑1 (MQ=255)


          gCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATcc                                                                                       <  1:3450019/101‑1 (MQ=255)


            aTCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCAt                                                                                     <  1:3688177/101‑1 (MQ=255)


             tCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATc                                                                                    >  2:1236583/1‑101 (MQ=255)


              cTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCt                                                                                   <  1:1489474/101‑1 (MQ=255)


              cTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCt                                                                                   <  1:446300/101‑1 (MQ=255)


              cTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACAGGATCGG.CTTTCGTATCCATCt                                                                                   <  1:171155/101‑1 (MQ=255)


               tGATCGCCGCGGCGCGGGCTGGGTTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTg                                                                                  <  2:2130436/101‑1 (MQ=255)


                gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                 <  1:4130956/101‑1 (MQ=255)


                gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                 >  1:4345732/1‑101 (MQ=255)


                gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                 <  2:2799584/101‑1 (MQ=255)


                gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                 <  1:101710/101‑1 (MQ=255)


                actCGCCGCGGCGCGGGCGGGGCTTTCGGTGATCCGGAACGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                 <  2:1117475/99‑1 (MQ=255)


                   cgcctcGGCGCGGGCGGGGCTTTCGCTGATCTTGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAtt                                                                              <  2:2237723/101‑1 (MQ=255)


                      cgcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                           <  2:4278120/101‑1 (MQ=255)


                      cgcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                           <  2:4050267/101‑1 (MQ=255)


                       gcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCa                                                                          >  1:3533245/1‑101 (MQ=255)


                        cggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAg                                                                         >  2:2090131/1‑101 (MQ=255)


                        cggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAg                                                                         >  1:3840092/1‑101 (MQ=255)


                          gcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGAt                                                                       <  2:576839/101‑1 (MQ=255)


                            gcgggcggGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGt                                                                     <  1:2860005/101‑1 (MQ=255)


                             cgggcgggGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGtt                                                                    <  1:4415196/101‑1 (MQ=255)


                             cgggcgggGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGtt                                                                    <  2:4254752/101‑1 (MQ=255)


                             cgggcgggGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGtt                                                                    <  2:1689981/101‑1 (MQ=255)


                              gggcggggCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                   >  2:611100/1‑101 (MQ=255)


                               ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                  >  1:2659034/1‑101 (MQ=255)


                                 cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                >  2:1646040/7‑101 (MQ=255)


                                 cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                <  2:1496053/95‑1 (MQ=255)


                                 cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                >  2:3454788/7‑101 (MQ=255)


                                  ggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACa                                                               >  1:3945183/6‑101 (MQ=255)


                                  ggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACa                                                               <  1:3728771/96‑1 (MQ=255)


                                   gggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAt                                                              >  2:4429858/5‑101 (MQ=255)


                                    ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                             <  1:1167306/98‑1 (MQ=255)


                                    ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                             <  1:4595032/98‑1 (MQ=255)


                                      ctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                           <  2:4333689/100‑1 (MQ=255)


                                       tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGcc                                                          <  2:4341896/101‑1 (MQ=255)


                                          cGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAg                                                       >  1:478053/1‑101 (MQ=255)


                                          cGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAg                                                       <  2:2729589/101‑1 (MQ=255)


                                           gCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAgg                                                      >  2:3686278/1‑101 (MQ=255)


                                             tGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTg                                                    >  1:4729813/1‑101 (MQ=255)


                                              gATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGc                                                   >  2:2215494/1‑101 (MQ=255)


                                              gATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGc                                                   >  1:1236574/1‑101 (MQ=255)


                                               aTCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GTGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCt                                                  <  1:4035806/101‑1 (MQ=255)


                                                  tGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGc                                               <  2:2872557/101‑1 (MQ=255)


                                                  tGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGc                                               >  2:3861554/1‑101 (MQ=255)


                                                  tGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGc                                               >  1:1296273/1‑101 (MQ=255)


                                                  tGGAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGc                                               <  2:1630234/101‑1 (MQ=255)


                                                    gAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCt                                             >  1:65833/1‑101 (MQ=255)


                                                    gAAGGTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCt                                             >  1:4185324/1‑101 (MQ=255)


                                                        gTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggc                                         <  2:1966925/101‑1 (MQ=255)


                                                        gTCGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggc                                         <  2:3151621/101‑1 (MQ=255)


                                                          cGTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcgg                                       <  1:3979597/101‑1 (MQ=255)


                                                           gTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                      >  2:543842/1‑101 (MQ=255)


                                                           gTGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGGGCTCGCCTGggcggc                                      >  2:4637788/1‑101 (MQ=255)


                                                            tGCTGGTGGTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcg                                     >  1:2232475/1‑101 (MQ=255)


                                                               tggtggTCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCt                                  <  2:414057/101‑1 (MQ=255)


                                                                  tggtCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTc                               >  2:2404766/1‑101 (MQ=255)


                                                                   ggtCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCg                              <  2:4496158/101‑1 (MQ=255)


                                                                     tCGAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTc                            <  2:3533026/101‑1 (MQ=255)


                                                                       gAATTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTg                          >  2:313807/1‑101 (MQ=255)


                                                                         aTTTCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGTGGCGGCTTTCGTCTGCg                        >  1:2078261/1‑101 (MQ=255)


                                                                          tttCTGG.GCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGt                       >  2:729363/1‑101 (MQ=255)


                                                                             ctgg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGAt                    <  1:611164/98‑1 (MQ=255)


                                                                             ctgg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGAt                    >  2:2040650/4‑101 (MQ=255)


                                                                              tgg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                   <  1:2554898/99‑1 (MQ=255)


                                                                              tgg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                   <  2:3839857/99‑1 (MQ=255)


                                                                              tgg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                   <  2:1748098/99‑1 (MQ=255)


                                                                              tgg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                   >  2:1422014/3‑101 (MQ=255)


                                                                               gg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGc                  <  1:164817/100‑1 (MQ=255)


                                                                               gg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGc                  >  2:2170195/2‑101 (MQ=255)


                                                                               gg.gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGc                  >  1:3386691/2‑101 (MQ=255)


                                                                                  gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTc                <  1:1189105/101‑1 (MQ=255)


                                                                                  gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTc                <  2:1555230/101‑1 (MQ=255)


                                                                                  gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTc                >  2:4612094/1‑101 (MQ=255)


                                                                                   ccGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCg               <  1:2024828/101‑1 (MQ=255)


                                                                                     gCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGcc             >  1:2736715/1‑101 (MQ=255)


                                                                                        cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc          >  2:1782819/1‑101 (MQ=255)


                                                                                        cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc          >  1:2271719/1‑101 (MQ=255)


                                                                                        cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCCCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc          <  1:3151358/101‑1 (MQ=255)


                                                                                         gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg         >  1:231799/1‑101 (MQ=255)


                                                                                         gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg         >  1:4678083/1‑101 (MQ=255)


                                                                                         gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg         >  1:2404956/1‑101 (MQ=255)


                                                                                         gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg         >  2:3330183/1‑101 (MQ=255)


                                                                                         gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg         <  2:2658880/101‑1 (MQ=255)


                                                                                            cGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAAt      <  1:1974259/101‑1 (MQ=255)


                                                                                            cGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAAt      >  1:1542974/1‑101 (MQ=255)


                                                                                              ggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATgg    >  1:2425329/1‑101 (MQ=255)


                                                                                              ggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCACGCCGTCGAATgg    >  2:1022572/1‑101 (MQ=255)


AGCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.G.GCCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCT  >  NC_014034/1786441‑1786636

Base quality scores:


ATCG

< 3 ≤


ATCG

< 24 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG