Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,057,828 Δ1 bp 10.7% intergenic (‑594/‑308) RCAP_rcc01900 ← / → RCAP_rcc01901 hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,057,8280G→.10.7% 54.8 ‑1.9 ‑0.2177intergenic (‑594/‑308)RCAP_rcc01900/RCAP_rcc01901hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (13/6):  ref base (76/82):  total (89/88)
Fisher's exact test strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.81e-01

Only 100 of 190 total aligned reads displayed.

GTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CCGCG.CGC.AGCG.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057732‑2057913
                                                                                                  |                                                                                         
gTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.Cc.cg.cgc                                                                                     >  2:4687075/1‑101 (MQ=255)
 tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.Cc.cg.cgcc                                                                                    >  1:2122288/1‑100 (MQ=255)
 tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.Cc.cg.cgcc                                                                                    >  1:1960980/1‑100 (MQ=255)
 tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.Cc.cg.cgcc                                                                                    >  1:1769594/1‑100 (MQ=255)
   cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCTATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGc                                                                                 >  1:4356268/1‑101 (MQ=255)
    tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGca                                                                                >  1:2186292/1‑100 (MQ=255)
    tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGca                                                                                >  1:1269161/1‑100 (MQ=255)
       ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.Cc.cg.cgccgagatc                                                                              >  1:2860671/1‑94 (MQ=255)
       ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagc                                                                             >  2:2893933/1‑97 (MQ=255)
         tccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgt                                                                           <  2:1317534/100‑7 (MQ=255)
          ccGCCCATTAACCCTGCTTCGGGGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgtt                                                                          <  1:1088807/101‑8 (MQ=255)
          ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgtt                                                                          <  2:3437825/101‑8 (MQ=255)
           cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgttt                                                                         >  2:3587988/1‑93 (MQ=255)
           cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgttt                                                                         <  1:2832507/101‑9 (MQ=255)
            gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.CG.CGC.CGcaagcgtttc                                                                        >  2:2756367/1‑92 (MQ=255)
                   aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCg                                                                     >  1:1775216/1‑101 (MQ=255)
                   aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGAGTGGCGTCAA.TCCCGCGCCGCAAGCGTTTCCGAACCg                                                                     <  2:3002515/101‑1 (MQ=255)
                    aCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCgg                                                                    >  1:1686709/1‑101 (MQ=255)
                     cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGc                                                                   >  2:1445076/1‑101 (MQ=255)
                      ccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCa                                                                  >  1:3019945/1‑101 (MQ=255)
                       cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCAt                                                                 <  2:229735/101‑1 (MQ=255)
                       cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCAt                                                                 <  2:1134113/101‑1 (MQ=255)
                       cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCAt                                                                 <  1:2109880/101‑1 (MQ=255)
                       cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCAt                                                                 <  2:481308/101‑1 (MQ=255)
                        tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATc                                                                <  2:3338291/101‑1 (MQ=255)
                        tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATc                                                                <  2:258385/101‑1 (MQ=255)
                         gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCa                                                               >  2:3128791/1‑101 (MQ=255)
                         gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCa                                                               <  1:883138/101‑1 (MQ=255)
                         gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCa                                                               >  2:546185/1‑101 (MQ=255)
                          cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCat                                                              <  2:3929740/101‑2 (MQ=255)
                          cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCat                                                              <  2:2145238/101‑2 (MQ=255)
                           ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCatt                                                             >  2:3956780/1‑99 (MQ=255)
                           ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCatt                                                             <  2:1797649/101‑3 (MQ=255)
                            tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTc                                                            >  1:3235399/1‑101 (MQ=255)
                             cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCt                                                           >  1:824865/1‑101 (MQ=255)
                             cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCt                                                           >  1:1043175/1‑101 (MQ=255)
                              ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCtt                                                          <  1:392945/101‑1 (MQ=255)
                              ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCtt                                                          <  1:568252/101‑1 (MQ=255)
                               gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTg                                                         <  2:892670/101‑1 (MQ=255)
                                cgcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTgg                                                        <  2:3657945/101‑1 (MQ=255)
                                   gcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccc                                                    <  2:3561699/101‑1 (MQ=255)
                                   gcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccc                                                    >  1:4585884/1‑101 (MQ=255)
                                    cgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccc                                                   >  2:4724051/1‑101 (MQ=255)
                                     ggccTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccc                                                  <  2:4571443/98‑1 (MQ=255)
                                     ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccc                                                  <  2:1224380/101‑1 (MQ=255)
                                     ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccc                                                  <  1:4468564/101‑1 (MQ=255)
                                      gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccccc                                                 <  2:2614122/101‑2 (MQ=255)
                                       aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccg                                                >  2:740208/1‑99 (MQ=255)
                                         ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                              >  2:530390/1‑97 (MQ=255)
                                         ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                              <  1:1345797/101‑5 (MQ=255)
                                          tCGGGGCGGCGGCGGGCCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgccg                                             <  2:3329062/101‑6 (MQ=255)
                                            ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCggg                                            >  1:4707809/1‑101 (MQ=255)
                                             gggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGa                                           <  1:1640761/101‑1 (MQ=255)
                                               gcggcggcgGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACa                                         <  1:635911/101‑1 (MQ=255)
                                                cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACaa                                        <  1:4279598/101‑1 (MQ=255)
                                                   cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCa                                     <  2:15036/101‑1 (MQ=255)
                                                    ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    <  2:1290873/101‑1 (MQ=255)
                                                    ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    <  1:3517525/101‑1 (MQ=255)
                                                       ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgc                                 >  2:3239815/1‑101 (MQ=255)
                                                       ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgc                                 >  1:1045976/1‑101 (MQ=255)
                                                          gCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGTACCGGCATCATTCTTGGCCCCCCCCCGGGCCAATCACGgccgc                              >  1:309420/1‑101 (MQ=255)
                                                           ctctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccgcc                             <  1:1249167/101‑1 (MQ=255)
                                                            tctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCt                            >  2:1185803/1‑101 (MQ=255)
                                                             ctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           >  1:398826/1‑101 (MQ=255)
                                                             ctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           >  1:2918187/1‑101 (MQ=255)
                                                             ctTGCGATCCTGCGTTTCCGGATGGGCGACAACC.CCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           <  2:2117701/101‑1 (MQ=255)
                                                              ttGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCa                          <  1:530441/101‑1 (MQ=255)
                                                               tGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  1:3378195/1‑101 (MQ=255)
                                                                 cGATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       >  1:267189/1‑101 (MQ=255)
                                                                  gATCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      >  2:2431759/1‑101 (MQ=255)
                                                                   aTCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGtt                     >  2:1354625/1‑101 (MQ=255)
                                                                   aTCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGtt                     <  1:4638308/101‑1 (MQ=255)
                                                                    tCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  1:3837664/1‑101 (MQ=255)
                                                                    tCCTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  2:4084153/1‑101 (MQ=255)
                                                                     ccTGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   <  2:1336364/101‑1 (MQ=255)
                                                                       tGCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGc                 >  1:21431/1‑101 (MQ=255)
                                                                        gCGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                <  2:4672040/101‑1 (MQ=255)
                                                                        gCGTTTCCGGATGGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                <  2:3764060/101‑1 (MQ=255)
                                                                         cGTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa               >  1:3665906/1‑101 (MQ=255)
                                                                          gTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  2:4381347/1‑101 (MQ=255)
                                                                          gTTTCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  1:3754788/1‑101 (MQ=255)
                                                                           tttCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             >  1:4206335/1‑101 (MQ=255)
                                                                           tttCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             >  2:4462882/1‑101 (MQ=255)
                                                                           tttCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             <  2:3722920/101‑1 (MQ=255)
                                                                            ttCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            >  2:3085795/1‑101 (MQ=255)
                                                                            ttCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            >  2:4480853/1‑101 (MQ=255)
                                                                             tCCGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGc           >  2:3845429/1‑101 (MQ=255)
                                                                              ccGGATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg          <  2:1846231/101‑1 (MQ=255)
                                                                                ggATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  2:2112568/1‑101 (MQ=255)
                                                                                ggATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  1:3458998/1‑101 (MQ=255)
                                                                                ggATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  1:755938/1‑101 (MQ=255)
                                                                                ggATTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  1:1144476/1‑101 (MQ=255)
                                                                                  aTTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      <  1:3723231/101‑1 (MQ=255)
                                                                                  aTTGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      >  1:2960935/1‑101 (MQ=255)
                                                                                   ttGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     >  2:3776007/1‑101 (MQ=255)
                                                                                   ttGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     <  1:4720311/101‑1 (MQ=255)
                                                                                   ttGGCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTCCGATCaa     >  2:3063622/1‑101 (MQ=255)
                                                                                     ggCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACGGGCATCATTCTTGGCCCCCCGCCGGGACAATCCAGGCCGCCGCATTGTTCCGCCATTGCGATCaacg   >  2:2021894/1‑99 (MQ=255)
                                                                                     ggCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAg   <  1:1552585/101‑1 (MQ=255)
                                                                                      gCGACAA.TCCCGCGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  >  2:2811096/1‑101 (MQ=255)
                                                                                                  |                                                                                         
GTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CCGCG.CGC.AGCG.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057732‑2057913

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG