Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,225 Δ1 bp 100% coding (914/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,2250A→.100.0% 862.0 195coding (914/1314 nt)RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (96/99):  ref base (0/0):  total (96/99)

Only 100 of 210 total aligned reads displayed.

AAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCT.GGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGC  >  NC_014034/2180128‑2180322
                                                                                                     |                                                                                                 
aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCTTGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                                                                  >  1:4117569/1‑100 (MQ=255)
 aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                                                                                                 >  1:3368635/1‑99 (MQ=255)
  ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                                                                <  1:2385236/101‑4 (MQ=255)
   gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                                                               <  2:1285087/101‑5 (MQ=255)
   gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                                                               <  1:2115324/101‑5 (MQ=255)
    aCTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                                                                                             >  1:4454033/1‑101 (MQ=255)
     cTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcgg                                                                                            >  1:3788892/1‑101 (MQ=255)
     cTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcgg                                                                                            >  2:1595195/1‑101 (MQ=255)
      tCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggc                                                                                           >  1:3902773/1‑101 (MQ=255)
       cGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattagatcg                                                                                           >  2:1343278/1‑93 (MQ=255)
       cGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcg                                                                                          >  1:1941572/1‑101 (MQ=255)
       cGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcg                                                                                          >  1:223941/1‑101 (MQ=255)
         gCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGAC.ATTCCGGCggg                                                                                        >  1:882569/1‑101 (MQ=255)
          ccGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                                                                       <  1:3766278/101‑1 (MQ=255)
            gTGGCCGATCATGTCGGCTTCGCGCTGGGG.CCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                                                                     >  2:1438911/1‑101 (MQ=255)
            gTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                                                                     <  1:1400896/101‑1 (MQ=255)
             tGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACg                                                                                    <  2:3954470/101‑1 (MQ=255)
             tGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACg                                                                                    >  2:2475028/1‑101 (MQ=255)
                ccGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                                                                 <  1:1758766/101‑1 (MQ=255)
                 cGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa                                                                                >  1:1275818/1‑101 (MQ=255)
                  gATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                                                               <  1:1300267/101‑1 (MQ=255)
                  gATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                                                               <  2:2946127/101‑1 (MQ=255)
                   aTCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                                                              >  2:1609840/1‑100 (MQ=255)
                   aTCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                                                              >  2:429729/1‑100 (MQ=255)
                     cATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                                                            >  1:4619315/1‑98 (MQ=255)
                      aTGTCGGCTTCGCGCT.GGCGCGGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                           >  1:2690611/1‑97 (MQ=255)
                      aTGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                           >  1:309877/1‑97 (MQ=255)
                      aTGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                           >  2:3622341/1‑97 (MQ=255)
                      aTGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                           <  1:453845/101‑5 (MQ=255)
                      aTGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                           >  1:4497651/1‑97 (MQ=255)
                       tGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                                                         <  2:574608/101‑1 (MQ=255)
                        gTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                                                                        <  1:2708192/101‑1 (MQ=255)
                        gTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                                                                        <  1:1439005/101‑1 (MQ=255)
                         tcggcttcgcgcT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg                                                                       <  2:3135368/101‑1 (MQ=255)
                          cggcttcgcgcT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                                                                      >  1:2522825/1‑101 (MQ=255)
                          cggcttcgcgcT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                                                                      >  2:2219595/1‑101 (MQ=255)
                          cggcttcgcgcT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                                                                      >  1:1056282/1‑101 (MQ=255)
                           ggcttcgcgcT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                                                                     >  1:1123126/1‑101 (MQ=255)
                           ggcttcgcgcT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                                                                     <  1:1020304/101‑1 (MQ=255)
                                   gct.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa                                                             >  2:4036359/1‑101 (MQ=255)
                                       ggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt                                                          >  1:3192748/5‑101 (MQ=255)
                                         cgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtc                                                        <  1:1523381/99‑1 (MQ=255)
                                         cgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtc                                                        >  1:732353/3‑101 (MQ=255)
                                          gccGAACAAGGTCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcg                                                       <  1:719268/100‑1 (MQ=255)
                                           ccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAGGCGGCGCTGAAATtcgt                                                      >  2:3685780/1‑101 (MQ=255)
                                            cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc                                                     <  2:12513/101‑1 (MQ=255)
                                            cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc                                                     <  1:3267137/101‑1 (MQ=255)
                                             gAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg                                                    >  2:1111508/1‑101 (MQ=255)
                                              aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                                   <  1:2748920/101‑1 (MQ=255)
                                                cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt                                                 >  1:1674022/1‑101 (MQ=255)
                                                cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt                                                 >  1:3511244/1‑101 (MQ=255)
                                                 aaggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATg                                                >  2:3871856/6‑101 (MQ=255)
                                                 aaggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCCCTGAAATTCGTCGAATg                                                >  2:3607230/6‑101 (MQ=255)
                                                  aggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg                                               <  2:1020366/97‑1 (MQ=255)
                                                     ccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCg                                            >  1:4264592/2‑101 (MQ=255)
                                                     ccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCg                                            <  2:4245430/100‑1 (MQ=255)
                                                      cGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGa                                           >  2:4415661/1‑101 (MQ=255)
                                                       ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc                                          >  1:3714283/1‑101 (MQ=255)
                                                         tgtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCt                                        <  1:4438712/95‑1 (MQ=255)
                                                          gtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTc                                       <  2:1757424/96‑1 (MQ=255)
                                                            cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGGCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                                     >  2:1521974/4‑101 (MQ=255)
                                                            cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                                     <  1:161129/98‑1 (MQ=255)
                                                            cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                                     <  1:3607449/98‑1 (MQ=255)
                                                            cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                                     <  2:3368429/98‑1 (MQ=255)
                                                             gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                                    >  2:1446125/3‑101 (MQ=255)
                                                             gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                                    <  2:2540434/99‑1 (MQ=255)
                                                              ccaggCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAg                                   >  2:4021923/5‑101 (MQ=255)
                                                                aaGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGa                                 >  2:4573868/1‑101 (MQ=255)
                                                                   cATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTa                              <  2:3831562/101‑1 (MQ=255)
                                                                    aTGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTac                             >  2:229377/1‑101 (MQ=255)
                                                                     tGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGCCTaca                            >  2:3940778/1‑101 (MQ=255)
                                                                        cAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCg                         <  2:1686695/101‑1 (MQ=255)
                                                                        cAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGACGCGGCGCTGAAATTCGCGCAATGGCCGCCCTCCAAGCCCTacactt                         >  1:2155471/1‑99 (MQ=255)
                                                                         aaCTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGc                        >  2:88622/1‑101 (MQ=255)
                                                                          aCTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGcc                       >  1:4649985/1‑101 (MQ=255)
                                                                          aCTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGcc                       <  2:4117327/101‑1 (MQ=255)
                                                                            tGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCt                     <  2:1842735/101‑1 (MQ=255)
                                                                             ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt                    <  2:47145/101‑1 (MQ=255)
                                                                             ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt                    <  2:4608690/101‑1 (MQ=255)
                                                                             ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt                    >  1:4751503/1‑101 (MQ=255)
                                                                               ctctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGt                  <  1:638052/101‑1 (MQ=255)
                                                                                tctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGGCCTCCAAGGACTACACCGCCCTTGTc                 >  2:744729/1‑101 (MQ=255)
                                                                                 ctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCg                <  2:3557718/101‑1 (MQ=255)
                                                                                 ctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCg                <  2:3788657/101‑1 (MQ=255)
                                                                                  tGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGc               >  2:3035184/1‑101 (MQ=255)
                                                                                   gggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGcc              <  2:2522680/101‑1 (MQ=255)
                                                                                   gggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGcc              <  2:1654686/101‑1 (MQ=255)
                                                                                    ggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCa             <  1:1997298/101‑1 (MQ=255)
                                                                                    ggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCa             <  2:2936699/101‑1 (MQ=255)
                                                                                     gCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAg            <  1:2272284/101‑1 (MQ=255)
                                                                                       cTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCa          >  2:1925288/1‑101 (MQ=255)
                                                                                        tGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaa         <  2:4506929/101‑1 (MQ=255)
                                                                                          ggaaccTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaag        <  2:2700683/97‑1 (MQ=255)
                                                                                           gaaccTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaagg       >  1:3057479/4‑101 (MQ=255)
                                                                                            gatcTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaagga      <  1:1307596/98‑1 (MQ=255)
                                                                                             accTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaaggaa     <  1:2898785/100‑1 (MQ=255)
                                                                                             accTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaaggaa     <  2:2323145/100‑1 (MQ=255)
                                                                                             accTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaaggaa     >  2:477306/2‑101 (MQ=255)
                                                                                               cTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaaggaagg   >  2:4626268/1‑101 (MQ=255)
                                                                                                tCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGc  >  2:3661723/1‑101 (MQ=255)
                                                                                                     |                                                                                                 
AAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCT.GGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGC  >  NC_014034/2180128‑2180322

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG