Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,474,914	Δ1 bp	100%	intergenic (‑77/+49)	RCAP_rcc02301 ← / ← engA	hypothetical protein/GTP‑binding protein EngA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,474,914	0	G→.	100.0%	118.6	36	intergenic (‑77/+49)	RCAP_rcc02301/engA	hypothetical protein/GTP‑binding protein EngA
Reads supporting (aligned to +/- strand):  new base (30/5):  ref base (1/0):  total (31/5)

GCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGGCGCAGCCCCCCGCGGGTC.GCCTCGGGCTTGCCCGAGGCGACATCAGCCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTTGCGTGCGGCGTG  >  NC_014034/2474814‑2475010                                                                                                     |                                                                                                  gCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCg                                                                                                   <  2:2993647/101‑1 (MQ=255) gCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCg                                                                                                   <  1:3398435/101‑1 (MQ=255) gCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCg                                                                                                   <  1:323983/101‑1 (MQ=255) gCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCg                                                                                                   <  1:2436835/101‑1 (MQ=255)       ccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggc                                                                                             <  2:1875540/101‑2 (MQ=255)        cGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcg                                                                                            <  1:699195/101‑3 (MQ=255)          ggATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcgca                                                                                          <  2:3812547/101‑5 (MQ=255)              atTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccc                                                                                     <  2:4455834/101‑1 (MQ=255)              atTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccc                                                                                     >  2:3495298/1‑101 (MQ=255)               tttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccc                                                                                    <  1:3758510/101‑1 (MQ=255)               tttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAAAACGCAAGGCGGGGGGGGC.GGGGGGCGCGGcccc                                                                                    >  1:459874/1‑101 (MQ=255)                ttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccccc                                                                                   <  2:4543219/101‑1 (MQ=255)                ttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccccc                                                                                   <  2:2673396/101‑1 (MQ=255)                 tCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccccc                                                                                  <  2:1863243/101‑1 (MQ=255)                   ccccAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGG.GCAGCCCCCcgc                                                                                >  1:4734003/1‑101 (MQ=255)                       aaGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGt                                                                            >  1:2366505/1‑101 (MQ=255)                        aGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGGGC.GGGGGGCGCAGCCCCCCGCggggg                                                                           >  1:533214/1‑99 (MQ=255)                            gACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGAGGTGC.GGGGGGCGCAGCCCCCCGCCGGGC.Gccc                                                                      >  1:4093433/1‑100 (MQ=255)                               aGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGAC.Gcccccg                                                                   >  2:3824668/1‑97 (MQ=37)                                ggAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGG.GCCCCggg                                                                  >  1:3353250/1‑101 (MQ=37)                                 gAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGC.CCCCCgggg                                                                 >  1:391673/1‑100 (MQ=37)                                  aaaCCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTG.Gcccccgggc                                                                >  1:2030598/1‑94 (MQ=37)                                         ggCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCGGCCCCGGGC.TGCCCgc                                                         >  2:4612823/1‑100 (MQ=25)                                           ccccccccACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTC.GCCCCGGGCCTGCCCGCgg                                                       >  1:728954/1‑101 (MQ=25)                                            cccccccACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGC.GCCTCGGGCTTGCCCGAggg                                                      >  2:4342022/1‑100 (MQ=255)                                               ccccACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGC.GGGCCTCGCTTGCCCgccggggc                                                   >  2:9762/1‑94 (MQ=21)                                                  cACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTC.GGCGCGGGGGTGCCCGCGGCgggaga                                                >  2:3210111/1‑96 (MQ=21)                                                          cGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTC.CCCTCGGGCTTGCGCGGCGCGACAGCAGCCCTTc                                        >  2:2665854/1‑101 (MQ=25)                                                                  cTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTC.GCCTCGGGCTTGCCCGAGGCGACATCAGCCCTTCTGTTTCGa                                >  2:2950801/1‑101 (MQ=255)                                                                        cTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTC.GGCTCGGGCTTGCCCGGGGCGCCAAACACCCTTCGGGTTccgtcgcgg                          >  2:4217951/1‑93 (MQ=17)                                                                        cTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTC.GCCTCGGGCTTGCCCGAGGCGACATCAGCCCTTCGGTTTCGAGGGCAg                          >  2:585738/1‑101 (MQ=255)                                                                        cTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTC.GCCTCCGGCGTGCCCCCGGCGACACCAGCCCCTCCGTCTTGAGGGGAg                          >  1:747432/1‑101 (MQ=18)                                                                                aaTACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTC.GCCTCGGGGTTGCCCGAGGGGGCATCAGCCCCTCGGTTTTGGGGGCAGGCCCtttt                  >  1:1247366/1‑99 (MQ=18)                                                                                 aTACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTC.GCCTCGGGGCTGCCCGAGGGGACATCAGCCCTTCGGGTTCGAGGGCAGGGCCtttac                 >  2:4707787/1‑98 (MQ=18)                                                                                  tACGCAAGGCGGGGGGTGC.GGCGGGCGCAGCCCCCCGCGGGGC.GCCTCGTGCTTGCCCGCGGCGGCCCCAGCCCTTCGGTTTCGAGGGCAGGCCCTTGTcc                >  1:2691379/1‑101 (MQ=255)                                                                                   aCGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGC.GCCCCGGGGTTGCCCGGGGGGGCATCCGCCCCTCGGGTTTGAGGGGAGGCCCCTGTTCt               >  1:3659889/1‑101 (MQ=12)                                                                                    cGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGC.GCCTCCCGCTTGCCCGACGCGACACCAGCCCTTCGGATTCGAGGGCCGGCCCTTGTccct              >  1:2285666/1‑99 (MQ=21)                                                                                            ggggggTGC.GGGGGGCGCAGCCCCCCGCGGGGC.GCCCCGGGGTTGGCCGGGGCGGCATCAGCCCCTCGGTTTTGGGGGGAGGCCCCTGTCCTTGCGGgcgg      >  1:4682131/1‑101 (MQ=16)                                                                                             gggggTGC.GGGGGGCGCAGCCCCCCGCGGGGC.GCCCCCGGCCTGGCCCCGGGGAAAACCGCCCCTCCGTTTCGTGGGGGGGCCCCTGTCCCTTCGTgcggg     >  2:950313/1‑100 (MQ=2)                                                                                              ggggTGC.GGGGGGCGCAGCCCCCCGCGGGGCGGCCCCGGGCTGGCCCCGGGGGCCACCCCCCCTTCGGGTTGTGGGGGGGGCCCTTTTCCtttgggcgggg     >  1:2397313/1‑92 (MQ=2)                                                                                               gggTGC.GGGGGGCGCAGCCCCCCGCGGGTC.GCCTCGGGGTTGCCCCAGGGGACATCAGCCCTTCGGGTTCGTGGGGAGGCCCCTGTCCTTGCGGggggcgg   >  1:1932064/1‑100 (MQ=18)                                                                                                ggTGC.GGGGGGCGCAGCCCCCCGCGGGTC.GCCTCGGGCTTGGCCCAGGCGGCATCAGCCCTTCCGGTTCGAGGGGAGGGCCCTGTCCTTGGCTgcgggcgt  >  2:1800633/1‑97 (MQ=12)                                                                                                ggTGC.GGGGGGCGCAGCCCCCCGCGGGTC.GCCTCGGGCTTGCCCGAGGCGACATCAGCCCTTCGGGTTCGAGGGCAGGCCCTTGTCCTTGCGTGCGGCgtg  >  2:2411523/1‑101 (MQ=255)                                                                                                ggTGC.GGGGGGCGCAGCCCCCCGCGGGGC.GCCTCGGGCTTGGCCCAGGGGAGATCAGGCCCTCGGGTTCGAGGGCAGGCCCTTGTCCCTGCGTgcggcggg  >  2:1474770/1‑99 (MQ=255)                                                                                                     |                                                                                                  GCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGGCGCAGCCCCCCGCGGGTC.GCCTCGGGCTTGCCCGAGGCGACATCAGCCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTTGCGTGCGGCGTG  >  NC_014034/2474814‑2475010 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG