Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 290,303 Δ1 bp 2.3% intergenic (‑36/+14) RCAP_rcc00253 ← / ← RCAP_rcc00254 major facilitator superfamily protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034290,3030G→. 2.3% 4.0 ‑0.5 ‑0.0175intergenic (‑36/+14)RCAP_rcc00253/RCAP_rcc00254major facilitator superfamily protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/3):  ref base (84/87):  total (85/90)
Fisher's exact test strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

AAGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAGCC  >  NC_014034/290203‑290403
                                                                                                    |                                                                                                    
aaGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCg                                                                                                      >  2:2169827/1‑101 (MQ=255)
aaGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGCGATCAGGCGGGCCTGGGTCATTTGGTCTCCCCCGCAAGGCGGCCAGTTCGCCATGCg                                                                                                      >  1:646894/1‑101 (MQ=255)
 aGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgg                                                                                                     <  2:940144/101‑1 (MQ=255)
 aGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGGCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgg                                                                                                     >  2:4104290/1‑101 (MQ=255)
  gCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCggg                                                                                                    >  2:598248/1‑101 (MQ=255)
  gCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCggg                                                                                                    >  2:1937112/1‑101 (MQ=255)
    cAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCggggg                                                                                                  >  1:34305/1‑101 (MQ=255)
      aGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCggggggg                                                                                                >  2:468867/1‑101 (MQ=255)
       gCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgggggggg                                                                                               <  1:2769744/101‑1 (MQ=255)
        cATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCggggggggg                                                                                              <  2:626926/101‑1 (MQ=255)
          tCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCt                                                                                            <  1:1725000/101‑1 (MQ=255)
            aaGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGc                                                                                          >  2:343107/1‑101 (MQ=255)
            aaGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGGCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAATTCGCCATGC.GGGGGGGGCTGCg                                                                                         >  2:73912/1‑101 (MQ=255)
             aGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgggggggggggcgc                                                                                         >  1:248097/1‑96 (MQ=255)
             aGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCg                                                                                         >  2:2292949/1‑101 (MQ=255)
               atgatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTc                                                                                       >  1:3536520/1‑101 (MQ=255)
               atgaggGCGCCGCCCACTAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTc                                                                                       <  2:4264649/101‑1 (MQ=255)
                tgatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCa                                                                                      >  1:1574546/1‑101 (MQ=255)
                tgatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCa                                                                                      <  1:3900267/101‑1 (MQ=255)
                 gatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTGaa                                                                                     >  1:1037120/1‑101 (MQ=255)
                 gatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCaa                                                                                     >  1:4615830/1‑101 (MQ=255)
                 gatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGGCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGCGGGGGGGCTGCGTCaa                                                                                     >  2:1324823/1‑101 (MQ=255)
                  atgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAAt                                                                                    <  1:1416771/101‑1 (MQ=255)
                  atgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAAt                                                                                    >  2:3384572/1‑101 (MQ=255)
                  atgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAAt                                                                                    <  1:3263968/101‑1 (MQ=255)
                  atgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCGGCGTCaac                                                                                    >  2:1356570/1‑100 (MQ=255)
                     gcgcCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTg                                                                                 <  1:4482821/101‑1 (MQ=255)
                       gccgcgCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCCCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTc                                                                               <  1:1798154/101‑1 (MQ=255)
                           cccACAAGGACGGGGGTGATCAGGCGGGTCTGGGTAATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGAtt                                                                           <  1:3943009/101‑1 (MQ=255)
                            ccACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATtg                                                                          >  2:3883188/1‑101 (MQ=255)
                            ccACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATtg                                                                          <  1:4301184/101‑1 (MQ=255)
                                aaGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGcc                                                                      >  2:3236033/1‑101 (MQ=255)
                                 aGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGccc                                                                     >  2:1896496/1‑101 (MQ=255)
                                 aGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGccc                                                                     >  1:409074/1‑101 (MQ=255)
                                  ggACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGcccc                                                                    >  2:689887/1‑101 (MQ=255)
                                     cGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGaa                                                                 <  1:670226/101‑1 (MQ=255)
                                     cGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGaa                                                                 <  1:2921964/101‑1 (MQ=255)
                                      gggggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAAc                                                                <  1:2096576/101‑1 (MQ=255)
                                      gggggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAAc                                                                <  1:2631933/101‑1 (MQ=255)
                                      gggggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAAc                                                                >  1:2231183/1‑101 (MQ=255)
                                      gggggTGATCAGGCGGGTCTGGGTCATTTGGTCCCCCTCGCAGGGCGGCCAGTTCGCCATGC.GGGGGGGGCTGCGTCAATCTGTCGATTGTTCCCCGAACa                                                               <  2:3698232/101‑1 (MQ=255)
                                       ggggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACa                                                               <  1:2283267/101‑1 (MQ=255)
                                       atcttGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACa                                                               <  2:2836187/97‑1 (MQ=255)
                                         ggTGATCAGGCGGGTCTGGGTCTTTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAAt                                                             >  1:4401806/1‑101 (MQ=255)
                                          gTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATc                                                            <  2:1638062/101‑1 (MQ=255)
                                          gTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGC.GGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATcg                                                           <  1:73919/101‑1 (MQ=255)
                                           tGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATcg                                                           >  2:648810/1‑101 (MQ=255)
                                           tGATCAGGCGGGTCTGGGGCATTTGGTCTCCCTCGCCAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATGTGTCGATTGTGCCCCGAACAATcg                                                           >  2:4720294/1‑101 (MQ=255)
                                            gATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATcgc                                                          >  1:3315766/1‑101 (MQ=255)
                                            gATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATcgc                                                          <  1:47956/101‑1 (MQ=255)
                                               cAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgcc                                                       >  2:4356614/1‑101 (MQ=255)
                                                aGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccg                                                      <  1:4697736/101‑1 (MQ=255)
                                                  gCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccgcc                                                    <  2:2406939/101‑1 (MQ=255)
                                                      gTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCt                                                <  1:2293082/101‑1 (MQ=255)
                                                       tCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAAACGCGCCGCCGCCTg                                               >  2:499972/1‑101 (MQ=255)
                                                        cTGGGTCATTGGGTCTCCCTCGCAAGGCGGCCAGGTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCCCCGCCGCCTGc                                              <  2:3536301/101‑1 (MQ=255)
                                                         tGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGcc                                             <  2:3597850/101‑1 (MQ=255)
                                                           ggTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCg                                           <  2:2569559/101‑1 (MQ=255)
                                                           ggTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCg                                           <  2:1153004/101‑1 (MQ=255)
                                                            gTCTTTTGGTCCCCCTCGCAGGGGGGCAGGTCCGCCAGGGGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCgg                                          <  1:159278/101‑1 (MQ=255)
                                                            gTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGGCCCGAAAAATCGCGCCGCCGCCTGCCCgg                                          >  2:3974232/1‑101 (MQ=255)
                                                            gTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCgg                                          >  1:3662055/1‑101 (MQ=255)
                                                              cATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCg                                        >  1:979664/1‑101 (MQ=255)
                                                              cATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCg                                        <  1:2595116/101‑1 (MQ=255)
                                                              cATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGC.GGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGa                                       <  2:2616059/101‑1 (MQ=255)
                                                               aTTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCgc                                       >  1:4026126/1‑100 (MQ=255)
                                                               aTTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGC.GGGGGGGGCTCCGTCGATCTGTCGATTGTTCCCCGAACACTCGCGGCGCCGCCTGCCCCGCGAc                                      >  1:1892010/1‑101 (MQ=255)
                                                                tttGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAc                                      <  1:4125435/101‑1 (MQ=255)
                                                                ttgggTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAc                                      <  2:3073593/98‑1 (MQ=255)
                                                                 ttGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcg                                     >  2:176701/1‑101 (MQ=255)
                                                                  tGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcgc                                    >  1:2832528/1‑101 (MQ=255)
                                                                   ggTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcgcg                                   <  2:4457948/101‑1 (MQ=255)
                                                                   ggTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcgcg                                   <  1:624361/101‑1 (MQ=255)
                                                                     tctcgttCGCAAGGCGGCCAGTTCGCCATTCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGc                                 <  2:1450597/95‑1 (MQ=255)
                                                                     tctcCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGc                                 >  1:3497768/1‑101 (MQ=255)
                                                                     tctcCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCCGCGTCAATCTGGCGGTTGTGTCCCTAACAATCGCGCACACGCCTGTCCGGCGAccgcac                                 >  2:575156/1‑96 (MQ=255)
                                                                       tcCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGccc                               >  1:3402946/1‑101 (MQ=255)
                                                                       tcCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGccc                               >  1:3050512/1‑101 (MQ=255)
                                                                       ccccTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGccc                               <  2:203178/100‑1 (MQ=255)
                                                                        cgcacgaaaGGCGACAAGTACGCCATGGGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCg                              <  1:2351841/94‑1 (MQ=255)
                                                                         ccTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGt                             >  2:3710421/1‑101 (MQ=255)
                                                                         ccTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGt                             <  2:1025077/101‑1 (MQ=255)
                                                                         ccTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGt                             >  2:542827/1‑101 (MQ=255)
                                                                             gCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGGCggc                         >  2:4331251/1‑99 (MQ=255)
                                                                               aaGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAg                       >  2:2196190/1‑101 (MQ=255)
                                                                                  gcggcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACa                    <  2:1100484/101‑1 (MQ=255)
                                                                                     gcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGcc                 >  1:3071646/1‑101 (MQ=255)
                                                                                     gcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGcc                 <  1:312650/101‑1 (MQ=255)
                                                                                        aGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCggc              >  2:440168/1‑100 (MQ=255)
                                                                                        aGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGCCGCCAGACAGCCggc              >  1:2717604/1‑100 (MQ=255)
                                                                                              ccATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTcagcag        <  1:542878/101‑1 (MQ=255)
                                                                                               cATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGa       >  1:1266774/1‑101 (MQ=255)
                                                                                               cATGAGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGa       >  2:4020243/1‑101 (MQ=255)
                                                                                                aTGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGAt      >  1:1213690/1‑101 (MQ=255)
                                                                                                aTGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGAt      <  2:26433/101‑1 (MQ=255)
                                                                                                aTGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGAGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGAt      >  1:779699/1‑101 (MQ=255)
                                                                                                 ggggggggggggCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATa     <  1:852059/98‑1 (MQ=255)
                                                                                                  gCGGGGGGGGGCTGCGTCAATCTGTCCATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAg    <  1:2854664/101‑1 (MQ=255)
                                                                                                   cGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCCGACAGCCGGTCAGCAGATAgc   >  2:2980675/1‑101 (MQ=255)
                                                                                                    gggggggggCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAgcc  >  1:406487/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
AAGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAGCC  >  NC_014034/290203‑290403

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG