Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,732,702	+GG	100%	intergenic (‑126/‑277)	RCAP_rcc01601 ← / → tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,732,702	1	.→G	100.0%	158.6	61	intergenic (‑126/‑277)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/60):  ref base (1/0):  total (1/60)
*	NC_014034	1,732,702	2	.→G	100.0%	160.5	62	intergenic (‑126/‑277)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/61):  ref base (1/0):  total (1/61)

TATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.A.CCGGGC..GGGCGGG..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCC  >  NC_014034/1732604‑1732780                                                                                                          ||                                                                                   tatataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCTGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCcgggc..g                                                                                   <  1:3990230/101‑1 (MQ=255)  atataGGGTAGCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCcgggc..gg                                                                                  <  1:1758649/101‑1 (MQ=255)    ataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGGCG.C.GGC.G.G.Cccggg..ggggg                                                                               >  1:1245027/1‑99 (MQ=255)    ataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCcgggc..gggg                                                                                <  1:2106962/101‑2 (MQ=255)    ataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCcgggc..gggg                                                                                <  1:2722218/101‑2 (MQ=255)    ataGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCCGCG.GGGGGGGGCCG.G.GGG.G.G.Cccggg..gggcg                                                                               >  1:4239943/1‑101 (MQ=255)      aGGGTATCGGGGGGAGAGGTAAAGGCCGTCAGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCcgggc..gggggc                                                                              <  1:2321907/101‑4 (MQ=255)       gggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCcgggc..gggggct                                                                             <  2:1208617/101‑5 (MQ=255)       gggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.A.CCCAGC..GGCAggg..g                                                                          >  2:2010519/1‑101 (MQ=255)        ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCcgggc..gggggctc                                                                            <  1:2600854/101‑6 (MQ=255)        ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCcgggc..gggggctc                                                                            <  1:3846090/101‑6 (MQ=255)        ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCcgggc..gggggctc                                                                            <  1:379186/101‑6 (MQ=255)        ggTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCcgggc..gggggctc                                                                            <  1:3050117/101‑6 (MQ=255)         gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCcgggc..gggggctcc                                                                           <  1:1362947/101‑7 (MQ=255)         gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGC..GGG.GGG..Gc.c                                                                       >  1:1515600/1‑101 (MQ=255)          tATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.A.CCCGG...GGGCggg..ggggg                                                                      >  1:4456864/1‑97 (MQ=255)          tATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCC.C.GGC.G.ACCCCGGG..GGGAGGG..Gc.c                                                                       >  1:4339694/1‑101 (MQ=255)          tATCGGGGGGAGAGGT.AAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CCGCGGGGGCGCC.GGG.GCC.CCGGGC..GGGGGGG..Gc.c                                                                       >  2:1761968/1‑101 (MQ=255)           aTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGGCG.CGGGG.G.G.CCCGGG..GGGCggg..gggg                                                                       >  2:1693934/1‑98 (MQ=255)           aTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCcgggc..gggggctccgc                                                                         <  1:4230550/101‑9 (MQ=38)           aTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCcgggc..gggggctccgc                                                                         <  1:2825662/101‑9 (MQ=38)           aTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.A.CCCGGG..GGGGGGG..Gc.gcc                                                                     >  1:2422236/1‑101 (MQ=255)            tCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCcgggc..gggggctccgcc                                                                        <  1:2177076/101‑10 (MQ=38)             cGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.A.CCCCGG..GGGGGGG..Gc.cgccc                                                                   >  2:4748721/1‑100 (MQ=255)              ggggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGGCGCCGGGGGGGCCG.G.GGG.G.C.Cccggg..gggggggcgggggg                                                                      >  2:851820/1‑94 (MQ=255)                     gagGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGC..GGGGGGG..CC.CCCCCCC.ccccc                                                           >  1:1382527/1‑101 (MQ=255)                      agGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.cccc                                                            <  1:2762770/101‑1 (MQ=25)                      agGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.cccc                                                            <  1:4515417/101‑1 (MQ=25)                      agGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.A.CCCGGC..GGGGGGGC.GC.CGCCCCC.cccccc                                                          >  2:3587627/1‑100 (MQ=255)                       ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGG..GGGGGGG..GC.GCCCCCC.ccccccc                                                         >  2:1945436/1‑99 (MQ=255)                       ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.ccccc                                                           <  1:3623634/101‑1 (MQ=25)                       ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.ccccc                                                           <  2:4512322/101‑1 (MQ=25)                       ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGC..GGGGGGGC.GC.CCCCCCC.cccccc                                                          >  1:4695890/1‑100 (MQ=255)                        gTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCg                                                          <  2:3273949/101‑1 (MQ=25)                          aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGc                                                        <  1:4638408/101‑1 (MQ=25)                          aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGc                                                        <  2:1630495/101‑1 (MQ=25)                          aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGC..GGGGGGG..GC.CCCCCCC.cccccccccc                                                      >  1:1474621/1‑96 (MQ=255)                          aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAACCAGGGTTTCGACG.CGGCGGGGCCG.G.GGC.G.C.CCCG....GGGCGGG..GG.CGCCGCA.cccccccccccac                                                   >  1:1442202/1‑93 (MQ=255)                           aaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCg                                                       <  1:2040863/101‑1 (MQ=25)                           aaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCg                                                       <  2:2217529/101‑1 (MQ=25)                           aaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCg                                                       <  1:1231764/101‑1 (MQ=25)                           aaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.A.CCCGGG..GGGGGGC..GC.CGCCCCC.ccccccccgccg                                                    >  2:2957807/1‑94 (MQ=255)                            aGGCCGTCGGTGCGGGGNGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGt                                                      <  2:1186845/101‑1 (MQ=25)                            aGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGt                                                      <  1:3176346/101‑1 (MQ=25)                             ggCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTa                                                     <  1:4603507/101‑1 (MQ=25)                             ggCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGC..GGGGGGG..CC.GCCCCCC.ccccccccgccgg                                                   >  2:440907/1‑93 (MQ=255)                              gCCGTCGGTGCGGGGCGGAAACCCGCGTGCCAAGCAGGGCTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCC.CCCCCGGCGTAt                                                    <  1:2067114/101‑1 (MQ=21)                               ccGTCGGTACGGGCCGGAAAGCCGCGTGCCAAGCAGCGTTTCGCCG.CGGTGGGGCCG.C.GGC.GCC.CCGGGCGGGGGCTCC..AC.CCCCGCA.CCCACGGCGTAtt                                                   <  2:3909308/101‑1 (MQ=255)                                cGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTAttt                                                  <  2:1110581/101‑1 (MQ=25)                                cGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTAttt                                                  <  2:4514785/101‑1 (MQ=25)                                cGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTAttt                                                  <  1:1160478/101‑1 (MQ=25)                                  tCGGTGCGGGGCGGAAAGCCGCGTGCCAAGGAGGGTTTCGCCG.CGGCGGGGCCG.C.GGCGG.C.CCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTgc                                                <  1:318127/101‑1 (MQ=21)                                  ggggTGGGGGGGGGAAGGCCGCGTGCCAGGCGGGGTTCCGCCG.GGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTgc                                                <  1:3278801/99‑1 (MQ=12)                                     gTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGcc                                             <  2:4362983/101‑1 (MQ=21)                                       gCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCaa                                           <  2:3933295/101‑1 (MQ=21)                                        cGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCaag                                          <  2:1679378/101‑1 (MQ=21)                                         ggggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCaaga                                         <  1:4670782/101‑1 (MQ=21)                                         ggggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCaaga                                         <  2:1822406/101‑1 (MQ=21)                                            gCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAagaaga                                      <  1:2910688/101‑1 (MQ=18)                                             cGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAgaagaa                                     <  2:1191509/101‑1 (MQ=18)                                             cGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAgaagaa                                     <  2:4344381/101‑1 (MQ=18)                                             cGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAgaagaa                                     <  1:1694039/101‑1 (MQ=18)                                             cGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAgaagaa                                     <  2:3644988/101‑1 (MQ=18)                                               gAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAAc                                   <  2:1554561/101‑1 (MQ=18)                                                aaaGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACa                                  <  2:410011/101‑1 (MQ=18)                                                 aaGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACaa                                 <  2:3332410/101‑1 (MQ=18)                                                    ccGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCg                              <  1:851894/101‑1 (MQ=18)                                                     cgcgTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCgg                             <  2:3385564/101‑1 (MQ=18)                                                     cgcgCGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGC..GGGGGGG..GCGCCCCCCACCCCCCCCCGGCATTACGCCAAAAAGAAACaaaaag                             >  2:1582373/1‑97 (MQ=255)                                                       ggTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGa                           <  1:1214685/100‑1 (MQ=18)                                                       cgTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGa                           <  2:1825693/101‑1 (MQ=18)                                                       cgTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGa                           <  1:3515664/101‑1 (MQ=18)                                                       cgTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGC..GGGGGGGCCGC.CCCCCCA.CCCCCCGCGGAATTGCGGCCAGAAGAAAAaaaagggg                           >  2:3013044/1‑95 (MQ=255)                                                        gTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGAc                          <  1:1454068/101‑1 (MQ=18)                                                         tGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCG.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGAcc                         <  2:3532047/101‑1 (MQ=18)                                                          gCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCa                        <  2:51330/101‑1 (MQ=18)                                                          gCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCa                        <  2:3419165/101‑1 (MQ=18)                                                           ccAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAg                       <  2:4475566/101‑1 (MQ=18)                                                            cAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAgg                      <  1:1996768/101‑1 (MQ=18)                                                             aaGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGc                     <  1:3839764/101‑1 (MQ=18)                                                               gCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.NCCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGccc                   <  1:19852/101‑1 (MQ=18)                                                                  gggTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccccc                <  1:1675706/101‑1 (MQ=18)                                                                   ggTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGCCCAGGCCCCCCt               <  1:2565323/101‑1 (MQ=18)                                                                   ggTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCt               <  2:895776/101‑1 (MQ=18)                                                                   ggTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCt               <  2:3586273/101‑1 (MQ=18)                                                                   ggTTTCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCt               <  1:2010636/101‑1 (MQ=18)                                                                     tttCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGc             <  2:2546499/101‑1 (MQ=18)                                                                      ttCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCa            <  1:1635440/101‑1 (MQ=18)                                                                       tCGCCG.CGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAg           <  2:4339447/101‑1 (MQ=18)                                                                        cgccg.cGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccgagagagtc          <  2:4548814/101‑10 (MQ=17)                                                                         gccg.cGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGcc         <  2:236966/101‑1 (MQ=18)                                                                          ccg.cGGCGGGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCg        <  1:1642167/101‑1 (MQ=18)                                                                              cggcggGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCAt     <  2:2383588/101‑1 (MQ=18)                                                                               ggcggGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATg    <  1:1945553/101‑1 (MQ=18)                                                                                gcggGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGc   <  1:1105428/101‑1 (MQ=18)                                                                                 gggggCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGcc  <  1:862395/100‑1 (MQ=18)                                                                                 cggGGCCG.C.GGC.G.ACCCGGGCGGGGGCTCC..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGcc  <  2:1043933/101‑1 (MQ=18)                                                                                                          ||                                                                                   TATATAGGGTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCG.CGGCGGGGCCG.C.GGC.G.A.CCGGGC..GGGCGGG..GC.CCCCGCA.CCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCC  >  NC_014034/1732604‑1732780 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG