BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,843,420	+C	100%	intergenic (+82/+66)	ppiB → / ← tyrS	peptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,843,420	1	.→C	100.0%	516.4	164	intergenic (+82/+66)	ppiB/tyrS	peptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): new base (87/77): ref base (0/0): total (87/77)

Only 100 of 175 total aligned reads displayed.


GGCCACGATCGAGTGACACGGGCGCA.GGGGGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.G.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCG  >  NC_014034/1843323‑1843512


ggCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgct                                                                                                   <  2:3888158/101‑3 (MQ=25)


ggCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgct                                                                                                   <  1:797951/101‑3 (MQ=25)


   cACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctccg                                                                                                <  1:4593070/101‑6 (MQ=25)


   cACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctccg                                                                                                <  2:426974/101‑6 (MQ=25)


    aCGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGc                                                                                               <  1:3582169/101‑1 (MQ=25)


         cGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gg.gg                                                                                        <  1:1443291/101‑1 (MQ=21)


            gggACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gg.gggcg                                                                                     <  1:1654839/99‑4 (MQ=21)


            gTGACGCGGGCGCAGGGGGGCCCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gg.gggcg                                                                                     <  1:2101307/101‑4 (MQ=18)


            gTGACACAGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGAATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gg.gggcg                                                                                     <  2:294181/101‑4 (MQ=21)


              gACACGGGCTCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gg.gggctcc                                                                                   <  1:4236482/101‑6 (MQ=18)


                   gggCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTc                                                                               <  2:2253713/101‑1 (MQ=21)


                   gggCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTc                                                                               <  2:3533597/101‑1 (MQ=21)


                    ggcggaggggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCa                                                                              <  2:3147639/94‑1 (MQ=18)


                    ggCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCa                                                                              <  2:1071665/101‑1 (MQ=21)


                    ggCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCa                                                                              <  1:973325/101‑1 (MQ=21)


                     gcgcAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCAt                                                                             <  1:2407920/101‑1 (MQ=21)


                     gcgcAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCAt                                                                             <  2:2702671/101‑1 (MQ=21)


                        caggggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCtt                                                                          <  1:2254479/98‑1 (MQ=21)


                         aggggtgCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTg                                                                         >  1:3833393/3‑101 (MQ=21)


                          ggggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGc                                                                        <  1:173567/100‑1 (MQ=21)


                           gggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGcc                                                                       >  2:2494720/1‑101 (MQ=21)


                             gggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCcac                                                                     <  2:1689265/101‑1 (MQ=25)


                              ggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCcaca                                                                    >  1:4421851/1‑101 (MQ=25)


                               gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACaa                                                                   <  1:3277040/101‑1 (MQ=25)


                                ctctG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACaaa                                                                  >  1:3711625/1‑101 (MQ=25)


                                  tctgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAat                                                                 >  1:4726029/5‑101 (MQ=25)


                                  tctgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAat                                                                 <  2:1874133/97‑1 (MQ=25)


                                   ctgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAata                                                                >  1:4551615/4‑101 (MQ=25)


                                      ccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATcc                                                             <  1:3181150/101‑1 (MQ=37)


                                       cccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATccc                                                            <  2:2610018/101‑1 (MQ=37)


                                       cccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATccc                                                            <  1:702714/101‑1 (MQ=37)


                                       cccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATccc                                                            <  2:1160879/101‑1 (MQ=37)


                                       cccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATccc                                                            <  2:281233/101‑1 (MQ=37)


                                          ccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCggg                                                         <  1:2355782/101‑1 (MQ=37)


                                           ctctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCgggg                                                        >  2:1473232/7‑101 (MQ=37)


                                            tctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGt                                                       >  1:3440640/6‑101 (MQ=38)


                                             ctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.c                                                     >  1:922650/5‑101 (MQ=38)


                                               gttgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.g                                                 >  1:3774306/3‑101 (MQ=38)


                                                ctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.g.g                                               >  2:3230767/2‑101 (MQ=38)


                                                  tctcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.g.ggg                                             <  1:3347170/98‑2 (MQ=38)


                                                    gcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.g.gggca                                           >  1:2799973/1‑98 (MQ=38)


                                                    gcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.g.gggca                                           <  2:1166402/101‑4 (MQ=38)


                                                    gcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGA.C.C.g.gggca                                           >  1:2281486/1‑98 (MQ=37)


                                                    gcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCAGGGT.C.C.g.gggca                                           >  2:2408065/1‑98 (MQ=37)


                                                     cATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.g.gggcag                                          >  2:4577133/1‑97 (MQ=37)


                                                     cATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.G.GGGaga                                          >  2:3346968/1‑101 (MQ=38)


                                                      aTTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.g.gggcaga                                         >  2:416216/1‑96 (MQ=37)


                                                      aTTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.g.gggcaga                                         >  1:162586/1‑96 (MQ=37)


                                                      aTTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.g.gggcaga                                         >  2:251849/1‑96 (MQ=37)


                                                      aTTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.GGAGGCGCCCTTTCATCTTGCTCCAAATATCCCGGGGT.C.C.g.gggcaga                                         >  1:3990381/1‑96 (MQ=25)


                                                       ttCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCagag                                         >  2:4461793/1‑101 (MQ=37)


                                                        tCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGc                                        >  2:3063181/1‑101 (MQ=37)


                                                         cACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGcc                                       >  2:3792446/1‑101 (MQ=25)


                                                         cACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGcc                                       >  1:1398677/1‑101 (MQ=25)


                                                          aCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGccc                                      >  2:3217510/1‑101 (MQ=25)


                                                           ccccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGcccc                                     <  1:2250166/101‑1 (MQ=25)


                                                               ccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGcc                                 >  1:3003270/1‑101 (MQ=25)


                                                                 gggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCtt                               <  2:4028375/101‑1 (MQ=25)


                                                                 gggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCtt                               <  2:162570/101‑1 (MQ=25)


                                                                 gggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCtt                               >  2:1973316/1‑101 (MQ=25)


                                                                   gATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.GCGGCAGAGCCCCGGCCTTGc                             >  2:4752458/1‑101 (MQ=25)


                                                                    atatTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCt                            >  1:2971938/1‑101 (MQ=25)


                                                                    atatTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCt                            >  2:1646148/1‑101 (MQ=25)


                                                                      atTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCa                          >  2:820350/1‑101 (MQ=25)


                                                                      atTTTCGCCAAGGTGAAATCGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCa                          >  2:133386/1‑101 (MQ=25)


                                                                       ttttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAg                         <  1:1067560/101‑1 (MQ=25)


                                                                        tttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGc                        >  1:1140124/1‑101 (MQ=25)


                                                                        tttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGc                        >  1:3769720/1‑101 (MQ=25)


                                                                         ttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGTCC.C.G.GGCAGCGCCCGGGCCTTGCTCGGcc                       >  2:3639364/1‑101 (MQ=25)


                                                                         ttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGcc                       >  2:2025402/1‑101 (MQ=25)


                                                                           cGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCTCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCa                     <  1:4688599/101‑1 (MQ=25)


                                                                            gCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCag                    <  2:4725773/101‑1 (MQ=25)


                                                                            gCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCag                    <  1:1399187/101‑1 (MQ=25)


                                                                             ccAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCaga                   >  2:2534817/1‑101 (MQ=25)


                                                                             ccAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCaga                   >  1:1314753/1‑101 (MQ=25)


                                                                              cAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCagag                  >  2:75915/1‑101 (MQ=25)


                                                                              cAAGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCagag                  >  2:2682759/1‑101 (MQ=25)


                                                                                aGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGac                <  1:1085996/101‑1 (MQ=25)


                                                                                aGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGac                >  2:3202563/1‑101 (MQ=25)


                                                                                aGGTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGac                <  2:4551362/101‑1 (MQ=25)


                                                                                 ggTGAAATGGAAAAGGGCGCTCCACTGGG.GGCACCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGaca               <  1:3120411/101‑1 (MQ=21)


                                                                                  gTGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGacac              <  2:410097/101‑1 (MQ=25)


                                                                                   tGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACAcc             <  1:2993333/101‑1 (MQ=25)


                                                                                   tGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACAcc             >  1:3863927/1‑101 (MQ=25)


                                                                                   tGAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACAcc             >  2:4624448/1‑101 (MQ=25)


                                                                                    gAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CAG.G.GGCAGAGCCCCGGCCTTGCTCAGCCCAgagaaaaca            >  1:551685/1‑96 (MQ=21)


                                                                                    gAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCa            <  2:3774070/101‑1 (MQ=25)


                                                                                    gAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCa            >  1:2037332/1‑101 (MQ=25)


                                                                                    gAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCa            <  1:1517069/101‑1 (MQ=25)


                                                                                    gAAATGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGAT.C.CGG.GGCAGCGCCCCGGCCTTGCTCAGCCCAGAGACACCa            >  2:4712618/1‑101 (MQ=25)


                                                                                     aaaTGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAg           <  2:3711395/101‑1 (MQ=25)


                                                                                       aTGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcg         >  2:3009464/1‑101 (MQ=25)


                                                                                        tGGAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcgc        >  1:61803/1‑101 (MQ=25)


                                                                                         ggAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCa       >  1:389895/1‑101 (MQ=25)


                                                                                         ggAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCa       >  1:3847120/1‑101 (MQ=25)


                                                                                         ggAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCa       >  1:4340734/1‑101 (MQ=25)


                                                                                         ggAAAAGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCa       >  1:854671/1‑101 (MQ=25)


                                                                                            aaaGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATgg    >  1:416228/1‑101 (MQ=25)


                                                                                            aaaGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATgg    >  2:3751531/1‑101 (MQ=25)


                                                                                              aGGGCGCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.CGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgcg  >  2:3830654/1‑101 (MQ=25)


GGCCACGATCGAGTGACACGGGCGCA.GGGGGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GG.GGCGCCCTTTCATCTTGCCACAAATATCCCGGGGT.C.C.G.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCG  >  NC_014034/1843323‑1843512

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 40 ≤


ATCG