BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,249,391	+C	92.4%	intergenic (‑187/‑118)	parE ← / → RCAP_rcc02080	DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,249,391	1	.→C	92.4%	32.6 ‑0.6 ‑1.4	172	intergenic (‑187/‑118)	parE/RCAP_rcc02080	DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand): new base (92/67): ref base (6/7): total (98/74)
Fisher's exact test strand distribution p-value = 5.62e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.81e-02

Only 100 of 220 total aligned reads displayed.


TGCCGGGACTCCCCCGGG.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.C.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTC  >  NC_014034/2249291‑2249485


tGCCGGGACTCCCCCGGG.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc.g                                                                                                     <  2:4149620/101‑1 (MQ=255)


 gCCGGGACTCCCCCGGG.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc.g.c                                                                                                   >  1:1325977/1‑101 (MQ=255)


      gACTCCCCCGGG.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.c.c.c.c.cc                                                                                          >  1:4029250/1‑100 (MQ=255)


      gACTCCCCCGGG.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.c.c.c.c.cc                                                                                          <  1:737818/101‑2 (MQ=255)


       gcTCCCCCGGG.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.c.c.c.c.cct                                                                                         <  2:245395/100‑3 (MQ=255)


       aCTCCCCCGGG.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.c.c.c.c.cct                                                                                         <  2:3656213/101‑3 (MQ=255)


        cccccccGGGAATTTTGGAGGAAAGAGGAGGGGG..GGTTTTCTTTTTGCAAAAATTACCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.c.c.c.c.cct                                                                                         <  1:3981906/99‑3 (MQ=12)


        cTCCCCCGGG.ATATTTGAGGCAAGATGAAGGGT..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.c.c.c.c.cctt                                                                                        <  1:2472012/101‑4 (MQ=255)


        cTCCCCCGGG.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.c.c.c.c.cctt                                                                                        <  1:3089351/101‑4 (MQ=255)


        cTCCCCCGGG.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.c.c.c.c.cctt                                                                                        <  1:3889935/101‑4 (MQ=255)


         tCCCCCGGG.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.c.c.c.c.ccttc                                                                                       <  2:3059914/101‑5 (MQ=255)


         tCCCCCGGG.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.c.c.c.c.ccttc                                                                                       <  1:1557905/101‑5 (MQ=255)


         gcccccGGG.ATATTTGGGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.c.c.c.c.ccttc                                                                                       <  1:4347372/100‑5 (MQ=255)


          cccccGTC.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.c.c.c.c.ccttcg                                                                                      <  1:756246/101‑6 (MQ=255)


          cccccGGG.ATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGC.G.C.C.C.C.Ct                                                                                          <  2:3851935/101‑1 (MQ=0)


           ccccGGG.ATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGC.G.C.C.C.C.Ctg                                                                                         >  2:4647477/1‑100 (MQ=0)


            cccGGG.ATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGC.G.C.C.C.C.Ctgc                                                                                        <  2:4544891/101‑3 (MQ=0)


            cccGGG.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTcgcg                                                                                     >  2:1272730/1‑101 (MQ=255)


              cGGG.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCgcggg                                                                                   >  1:851451/1‑101 (MQ=255)


               ggg.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCgcgggc                                                                                  >  2:487361/1‑101 (MQ=255)


                   atatTTGAGGCAAGATGAAGGGG..GGCTTTCACCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGggcggc                                                                               >  1:4206156/1‑101 (MQ=255)


                    tatTTGAGGCAAGATGAGGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGcc                                                                              <  2:3019066/101‑1 (MQ=255)


                    tatTTGAGGCAAGAGGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGcc                                                                              <  1:3167913/101‑1 (MQ=255)


                     atTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGccc                                                                             >  1:106802/1‑100 (MQ=255)


                     atTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCcg                                                                             >  2:2656498/1‑101 (MQ=255)


                      tttGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATGTCCCGGGGGGTGAA.TGGCGGA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCcgc                                                                            <  1:2774647/101‑1 (MQ=37)


                        tGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGa                                                                          >  1:170387/1‑101 (MQ=255)


                        tGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGa                                                                          >  1:3362466/1‑101 (MQ=255)


                        tGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGa                                                                          >  1:1903821/1‑101 (MQ=255)


                              aaGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGAcgcgcg                                                                    >  2:1824702/1‑101 (MQ=255)


                               aGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACgcgcgc                                                                   >  2:3871958/1‑101 (MQ=255)


                                 aTGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGGGGCCGCGACGCGggccg                                                                 >  1:2914682/1‑101 (MQ=25)


                                 aTGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGcgccg                                                                 >  2:3922272/1‑101 (MQ=255)


                                  tGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGccccac                                                                >  2:3969880/1‑99 (MQ=255)


                                     aGGGG..GGCTTTCATCTTGCCGAAAATATCGCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                             <  2:334307/101‑1 (MQ=255)


                                     aGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGCCGCGCGCCGCAAc                                                             >  1:2214648/1‑101 (MQ=255)


                                     aGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                             >  1:2704383/1‑101 (MQ=255)


                                     aGGGG..GGCTTTCATCTCGCAGAAAATAGCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                             <  1:230433/101‑1 (MQ=37)


                                      gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                                            <  1:2747345/101‑1 (MQ=255)


                                       ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                                                           >  2:658265/1‑101 (MQ=255)


                                        gg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                                                          >  2:4531718/1‑101 (MQ=255)


                                         g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.C.C.CGC.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt                                                         >  2:243587/1‑101 (MQ=255)


                                              cTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGt                                                      >  1:2047668/1‑101 (MQ=255)


                                                ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                                                    >  2:1795035/1‑101 (MQ=255)


                                                  cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                                  <  2:358880/101‑1 (MQ=255)


                                                  cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                                                  >  2:2856412/1‑101 (MQ=255)


                                                   aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                                 >  2:3437060/1‑101 (MQ=255)


                                                   aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                                 >  2:4121141/1‑101 (MQ=255)


                                                     cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                               <  1:1135972/101‑1 (MQ=255)


                                                     cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                               >  1:1769054/1‑101 (MQ=255)


                                                      ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCAAGAGGGGGGAAGA.G.CAA.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                              >  1:607164/1‑101 (MQ=38)


                                                        gCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt                                            <  1:4658713/101‑1 (MQ=255)


                                                         cgaaaaaTATCGCGGGGGGTGAA.GGGCGCA..GCCAGGGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                                           <  2:1688591/98‑1 (MQ=38)


                                                          cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                                          >  1:3733537/1‑101 (MQ=255)


                                                           gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                         <  1:4294672/101‑1 (MQ=255)


                                                            aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                                        <  1:3643399/101‑1 (MQ=255)


                                                            aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.C.CAC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                                        >  2:1904205/1‑101 (MQ=255)


                                                              aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                                      <  1:2082068/101‑1 (MQ=255)


                                                              aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                                      >  2:4167126/1‑101 (MQ=255)


                                                               atatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg                                     >  1:4587163/1‑101 (MQ=255)


                                                                   cgcGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc                                 <  2:4243666/99‑1 (MQ=255)


                                                                   cccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGCCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc                                 >  1:4228912/1‑101 (MQ=255)


                                                                      ggggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCGGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                              >  2:2810997/1‑101 (MQ=255)


                                                                      ggggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                              >  2:1074043/1‑101 (MQ=255)


                                                                       gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                             >  2:916540/1‑101 (MQ=255)


                                                                       gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                             >  1:2543109/1‑101 (MQ=255)


                                                                       gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                             >  1:4103246/1‑101 (MQ=255)


                                                                        ggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                            <  2:2285914/101‑1 (MQ=255)


                                                                        ggggTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                            >  2:3721327/1‑101 (MQ=255)


                                                                         gggTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                           >  1:2405869/1‑101 (MQ=255)


                                                                         gggTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                           <  2:2206508/101‑1 (MQ=255)


                                                                          ggTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg                          >  1:3321197/1‑101 (MQ=255)


                                                                          ggTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg                          >  2:239263/1‑101 (MQ=255)


                                                                           gTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt                         >  2:1314970/1‑101 (MQ=255)


                                                                           gTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt                         >  1:2161967/1‑101 (MQ=255)


                                                                             gAA.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa                       >  2:4012377/1‑101 (MQ=255)


                                                                              aa.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                      >  2:68389/1‑101 (MQ=255)


                                                                              aa.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                      <  1:3586526/101‑1 (MQ=255)


                                                                               aacggCGCA..GCCAGAGGGGGGCAGC.G.C.C.CTC.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                      <  2:233032/98‑1 (MQ=255)


                                                                               a.TGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa                     <  2:4355028/101‑1 (MQ=255)


                                                                                 tGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa                    >  2:1160350/1‑101 (MQ=255)


                                                                                 tGGCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa                    >  1:1448251/1‑101 (MQ=255)


                                                                                  ggCGCA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAg                   >  2:836456/1‑101 (MQ=255)


                                                                                    cgcA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCt                 <  2:811978/101‑1 (MQ=255)


                                                                                    cgcA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCt                 >  1:1215679/1‑101 (MQ=255)


                                                                                     gcA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTg                <  1:4380327/101‑1 (MQ=255)


                                                                                     gcA..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTg                >  1:685165/1‑101 (MQ=255)


                                                                                      cA..GCGAGGGGGGGGCTCCTG.C.T.C.C.GTTTGCGGGCAGCTGCGGCGCGCGTCGCTAAAGGTGGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg               <  2:1396445/101‑1 (MQ=255)


                                                                                       a..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGc              <  1:1737718/101‑1 (MQ=255)


                                                                                       a..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGc              >  1:99195/1‑101 (MQ=255)


                                                                                       a..GCCAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGc              >  2:2151677/1‑101 (MQ=255)


                                                                                            cAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc           >  1:2450390/1‑101 (MQ=255)


                                                                                            cAGAGGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc           >  1:3520520/1‑101 (MQ=255)


                                                                                             agagGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCa          >  2:260490/1‑101 (MQ=255)


                                                                                              gagGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAt         >  1:2146416/1‑101 (MQ=255)


                                                                                               agGGGGGCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCGGCATc        >  1:4153838/1‑101 (MQ=255)


                                                                                                  ggggCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGAt     <  2:1325885/101‑1 (MQ=255)


                                                                                                     gCAGC.GCC.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTc  >  2:4247014/1‑101 (MQ=255)


                                                                                                     gCAGC.GCC.C.C.C.CTTCGCGCGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTc  <  2:3131036/101‑1 (MQ=255)


                                                                                                     gCAGC.G.C.C.C.CGCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTc  <  2:1986648/101‑1 (MQ=255)


TGCCGGGACTCCCCCGGG.ATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGC.G.C.C.C.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTC  >  NC_014034/2249291‑2249485

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 22 ≤


ATCG

< 32 ≤


ATCG

< 39 ≤


ATCG