BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,566,557	+G	100%	intergenic (+29/+165)	purD → / ← sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,566,557	1	.→G	100.0%	459.0	172	intergenic (+29/+165)	purD/sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand): new base (44/125): ref base (1/0): total (46/126)

Only 100 of 202 total aligned reads displayed.


ATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.GGGG.G.CGCTGCCCCC.CTC.CT..G..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCC  >  NC_014034/2566458‑2566644


      tGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..ggccttc                                                                                     <  2:3120128/101‑7 (MQ=38)


       ggTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..ggccttcg                                                                                    <  1:1347379/101‑8 (MQ=38)


        gTCGACGCGATCGTCTGGCACGAGGGGTTCTGCCGCCGCGACAGCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..ggccttcgg                                                                                   <  2:724236/101‑9 (MQ=25)


          cGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..ggccttccgcc                                                                                 >  1:1942831/1‑91 (MQ=38)


          cGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGcc                                                                                 <  1:3105290/101‑1 (MQ=37)


             tGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAAt                                                                              <  1:621101/101‑1 (MQ=37)


               cGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.GGGTGCCCCC.CCC.TT..GGCCTTCGGCCCATTc                                                                            >  1:2241320/1‑101 (MQ=255)


                gATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCa                                                                           <  2:3008801/101‑1 (MQ=37)


                gATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCa                                                                           <  1:400313/101‑1 (MQ=37)


                 aTCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCAc                                                                          <  2:4250848/101‑1 (MQ=37)


                  tCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCAcc                                                                         <  1:3710347/101‑1 (MQ=37)


                       tGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCACTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCg                                                                    <  1:3484577/101‑1 (MQ=25)


                        ggCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCgg                                                                   <  2:2107644/101‑1 (MQ=25)


                        ggCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCgg                                                                   <  1:1271329/101‑1 (MQ=25)


                         gCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCggg                                                                  <  1:4648669/101‑1 (MQ=25)


                          cccGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGa                                                                 <  2:3361022/101‑1 (MQ=25)


                           ccGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCCCTGAGACG.AAACGGGGG.G.GGCTGCCCCC.CCC.TT..GGCCTTCGGCCAACTCACCCCCCggcgt                                                                >  1:2260402/1‑98 (MQ=255)


                            cGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGata                                                               >  2:402940/1‑101 (MQ=25)


                             gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGatat                                                              <  1:3598415/101‑1 (MQ=25)


                             gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGatat                                                              <  2:4448925/101‑1 (MQ=25)


                             gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGatat                                                              <  2:4552337/101‑1 (MQ=25)


                             gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGatat                                                              <  1:2293697/101‑1 (MQ=25)


                               ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATAttt                                                            <  2:137329/101‑1 (MQ=21)


                               ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATAttt                                                            <  2:1119688/101‑1 (MQ=21)


                                 ggTTCTTCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCg                                                          <  1:777211/101‑1 (MQ=21)


                                 ggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCg                                                          <  1:2504029/101‑1 (MQ=21)


                                  gTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCgg                                                         <  2:3424315/101‑1 (MQ=21)


                                   ttCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCCGCCCATTCACCCCCCcgggcaattttgg                                                        >  1:863966/1‑91 (MQ=19)


                                     cTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCa                                                      <  2:3220211/101‑1 (MQ=21)


                                      tGCCGCCGCGACATCGGCTGGCGCGCGCTCTGCGACGAAAAG.GGGG.G.CGGCGCCCCC.CTC.TT..GGCCTTCGGCCCATTCACCCCCCGGGAGAATTCCGGCaa                                                     >  2:1448697/1‑101 (MQ=16)


                                       gccgccGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAg                                                    <  2:3584721/101‑1 (MQ=21)


                                       gccgccGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGCCaga                                                    >  2:3688659/1‑99 (MQ=21)


                                        ccgccgCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGa                                                   <  1:2875269/101‑1 (MQ=21)


                                          gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTCTTT..GC.CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                                 >  2:3639782/1‑101 (MQ=21)


                                          gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                                 <  1:2697250/101‑1 (MQ=21)


                                          gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                                 <  2:500633/101‑1 (MQ=21)


                                          gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                                 <  1:2919991/101‑1 (MQ=21)


                                          gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                                 <  2:3280760/101‑1 (MQ=21)


                                           ccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaa                                                <  2:3892920/101‑1 (MQ=21)


                                            cgcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaaa                                               <  1:415380/101‑1 (MQ=21)


                                            cgcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaaa                                               <  2:2464653/101‑1 (MQ=21)


                                              cgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaaga                                             <  2:1755928/101‑1 (MQ=21)


                                              cgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaaga                                             <  2:1051078/101‑1 (MQ=21)


                                               gACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCCATTCACCCCCCGGGATATTTCGGGCAAGATAaagaa                                            >  1:2991139/1‑101 (MQ=21)


                                                aCATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaagaag                                           <  1:2399048/101‑1 (MQ=21)


                                                aCATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaagaag                                           <  1:4448661/101‑1 (MQ=21)


                                                 cATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAgg                                          <  2:4613648/101‑1 (MQ=21)


                                                  aTCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGt                                         <  1:1860053/101‑1 (MQ=21)


                                                   tCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTc                                        <  1:3907223/101‑1 (MQ=21)


                                                    cGGCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCt                                       <  1:715839/101‑1 (MQ=21)


                                                     ggCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtt                                      <  1:1065005/101‑1 (MQ=21)


                                                     ggCTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCtt                                      >  1:631974/1‑101 (MQ=21)


                                                       cTGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                                    <  1:4716385/101‑1 (MQ=21)


                                                        tGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                                   <  2:2288983/101‑1 (MQ=21)


                                                        tGGCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                                   <  1:3839415/101‑1 (MQ=21)


                                                         ggCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                                  <  2:3467003/101‑1 (MQ=21)


                                                         ggCGCGCGCTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                                  <  1:198833/101‑1 (MQ=21)


                                                         ggCGCGCGCTATGAGACG.AGAC.GGGG.GCCGCTGCCCCC.CTC.TTGGG..CTTCGGCCAATTCTCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                                  <  2:875624/101‑1 (MQ=21)


                                                           cgcgcgcTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                <  2:1685975/101‑1 (MQ=21)


                                                           cgcgcgcTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                <  2:2227890/101‑1 (MQ=21)


                                                           cgcgcgcTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                <  2:487191/101‑1 (MQ=21)


                                                           cgcgcgcTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                <  1:2036391/101‑1 (MQ=21)


                                                            gcgcgcTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                               <  2:1839728/101‑1 (MQ=21)


                                                            gcgcgcTCTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                               <  2:2717771/101‑1 (MQ=21)


                                                             cgcgcCGTGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TTA.GC.CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                              <  1:4677340/101‑1 (MQ=18)


                                                                 ctctGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCt                          >  2:4182655/1‑101 (MQ=21)


                                                                   ctGAGACG.AACC.GGGGCG.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                        <  2:620015/101‑1 (MQ=21)


                                                                   ctGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                        <  2:2156545/101‑1 (MQ=21)


                                                                    tGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGGTATTTGCGGCAAGATAAAGAAAGGCTTTTCCTTTCATCtttc                       >  2:4047009/1‑99 (MQ=17)


                                                                    tGAGACG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAAATCACCCCCCGGGACACTTCGTGCAACACGAAAAAGGGATTTTTCTTTTATCttcc                       >  1:4520337/1‑99 (MQ=2)


                                                                     gagaCG.AAACGGGGG.G.CGCTGCCCCC.CTCTTT..GC.CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGcc                      <  1:1485951/101‑1 (MQ=21)


                                                                     gagaCG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGcc                      <  2:3940742/101‑1 (MQ=21)


                                                                     gagaCG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCCGGGTATTTTTGGCAAAAAAAAAAAAGGGTTTTTCTTTTCTCttttc                      >  1:1691504/1‑98 (MQ=2)


                                                                      agaCG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccc                     <  2:1243486/101‑1 (MQ=21)


                                                                       gaCG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGAAATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccca                    >  2:1883798/1‑100 (MQ=21)


                                                                        aCG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCCGCATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGa                   >  2:2699577/1‑101 (MQ=21)


                                                                         cG.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaa                  <  1:4319021/101‑1 (MQ=21)


                                                                          g.AAACGGGGG.G.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                 >  1:4378135/1‑101 (MQ=21)


                                                                          cgaaccggggg.g.CCCGGCCCCC.CTC.TTG.G..CTTTCGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                 <  1:3492803/94‑1 (MQ=21)


                                                                            aaacggggg.g.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAat                <  1:1396642/96‑1 (MQ=21)


                                                                             aacggggg.g.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTGCCCGAAata               >  1:552176/5‑101 (MQ=21)


                                                                              acgtggg.g.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat              <  1:2573218/97‑1 (MQ=25)


                                                                               cagggg.g.CGCTGCCCCCTCTT.GG..C..CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggg       >  2:2979150/3‑101 (MQ=0)


                                                                               cagggg.g.CGCTG.CCCC.CTC.TT..GGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggg       >  2:506050/3‑101 (MQ=0)


                                                                                ggggg.g.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATcc            <  1:300878/100‑1 (MQ=25)


                                                                                ggggg.g.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGGGATTTTGGGGCAGAGAAAAAAAGGGTTTTTCTTTTTTTTTTCCCCCAATATcc            >  1:984749/2‑101 (MQ=2)


                                                                                ggggg.g.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATcc            <  2:3403742/100‑1 (MQ=25)


                                                                                ggggg.g.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATcc            >  2:2639398/2‑101 (MQ=25)


                                                                                agggg.g.CGCTG.CCCC.CTC.TT..GGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg      >  1:4604638/2‑101 (MQ=0)


                                                                                agggg.g.CGCTG.CCCC.CTC.TT..GGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg      >  1:2800801/2‑101 (MQ=0)


                                                                                 gggg.g.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc           >  2:1746777/1‑101 (MQ=25)


                                                                                 gggg.g.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc           <  1:4572076/101‑1 (MQ=25)


                                                                                  ggg.g.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCg          <  1:3343035/101‑1 (MQ=25)


                                                                                   gg.g.CGCTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCgg         <  1:4687976/101‑1 (MQ=37)


                                                                                      g.cgcTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCgggg       >  2:4465774/1‑101 (MQ=37)


                                                                                      g.cgcTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCgggg       <  1:4436796/101‑1 (MQ=37)


                                                                                      g.cgcTGCCCCC.CTC.TT..GGCCTGCGGCCTAGTCTCCCCCCGGGATATTTCGGGCAATATAAAGAAGGTCTTTTCCTTTCATCTTGCACGAAATATCCCgggg       <  2:1636853/101‑1 (MQ=21)


                                                                                        cgcTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCggggg      <  1:4182901/101‑1 (MQ=37)


                                                                                         gcTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGa     >  2:1913922/1‑101 (MQ=37)


                                                                                          cTGCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGGCAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAg    >  2:2414426/1‑101 (MQ=25)


ATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.GGGG.G.CGCTGCCCCC.CTC.CT..G..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCC  >  NC_014034/2566458‑2566644

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG