Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,160 +C 100% intergenic (‑136/+140) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1601.→C100.0% 401.8 131intergenic (‑136/+140)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (26/105):  ref base (0/0):  total (26/105)

Only 100 of 151 total aligned reads displayed.

CCGGTTGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACG  >  NC_014034/2640060‑2640256
                                                                                                       |                                                                                                
   gTTGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGG.cct                                                                                               <  1:4144671/101‑3 (MQ=255)
    ttGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGG.cctt                                                                                              <  1:1837501/101‑4 (MQ=255)
     tGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGG.ccttc                                                                                             <  2:4597273/101‑5 (MQ=255)
      gCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGG.ccttcg                                                                                            <  2:1377890/101‑6 (MQ=255)
          gTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGCGG.CCCCCCCC.TTTG.ccctccgccc                                                                                        >  1:430417/1‑92 (MQ=255)
          gTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCa                                                                                         <  2:174379/101‑1 (MQ=255)
          gTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCa                                                                                         <  1:4668069/101‑1 (MQ=255)
          gTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGG.cccttgggca                                                                                        >  1:1765513/1‑92 (MQ=255)
           ttATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCaa                                                                                        <  1:1534338/101‑1 (MQ=255)
           ttATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCaa                                                                                        <  1:3254962/101‑1 (MQ=255)
            tATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAAt                                                                                       <  1:747523/101‑1 (MQ=255)
            tATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAAt                                                                                       <  1:4542862/101‑1 (MQ=255)
             atgatgCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAAtt                                                                                      <  2:2456368/101‑1 (MQ=255)
               gatgCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTca                                                                                    <  2:793427/101‑2 (MQ=255)
               gatgCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTca                                                                                    <  1:4169620/101‑2 (MQ=255)
                  gcgcCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCAccc                                                                                 <  2:4229493/101‑1 (MQ=255)
                   cgcCGGGTATGCGACGCNGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCAcccc                                                                                <  2:1979548/101‑1 (MQ=255)
                   cgcCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTTGC.CTTCCGCCCAtttccctc                                                                                >  2:380262/1‑95 (MQ=37)
                    gcCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCAccccc                                                                               <  2:4035110/101‑1 (MQ=255)
                     ccGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCAcccccc                                                                              <  1:3504033/101‑1 (MQ=255)
                      cGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCTAATTCCCCCCCCg                                                                             <  1:2070680/101‑1 (MQ=37)
                        ggTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAg                                                                           <  1:2650889/101‑1 (MQ=255)
                          tATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGt                                                                         <  2:1228405/101‑1 (MQ=255)
                           aTGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTa                                                                        <  2:708431/101‑1 (MQ=255)
                            tGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCTCCCGAGGTAt                                                                       <  1:2646614/101‑1 (MQ=37)
                             gCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTAtt                                                                      <  1:1056033/101‑1 (MQ=255)
                             gCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTAtt                                                                      <  1:1167135/101‑1 (MQ=255)
                             gCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTAtt                                                                      <  1:809415/101‑1 (MQ=255)
                              ggACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTAttt                                                                     <  1:3689283/100‑1 (MQ=255)
                              cGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTAttt                                                                     <  2:1958958/101‑1 (MQ=255)
                              cGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTAttt                                                                     <  1:1413955/101‑1 (MQ=255)
                               gaggcggcgGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCGCCCCCCGAGGTATTTg                                                                    <  2:2556442/98‑1 (MQ=255)
                                aCGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTgg                                                                   <  1:218594/101‑1 (MQ=255)
                                    gggggcCGGCGTGTCAACCGTTGCGCTTTTGCGCAGGCAAAGGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCa                                                               <  1:1490855/98‑1 (MQ=21)
                                    ggcgggAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCa                                                               <  2:3254120/101‑1 (MQ=25)
                                     gcggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCaa                                                              <  1:2778093/101‑1 (MQ=25)
                                      gggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAg                                                             <  2:3368113/100‑1 (MQ=37)
                                      cggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGACGGATTTGGAGCAAg                                                             >  2:2352871/1‑101 (MQ=25)
                                       ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                            <  2:2917760/101‑1 (MQ=37)
                                       ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                            <  1:1966229/101‑1 (MQ=37)
                                        gcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTTGG.CTTCGGCCCATTTACCCCCCCAGGTAttttgggcaaaga                                                           >  2:1361193/1‑91 (MQ=25)
                                         cAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                          <  1:3759899/101‑1 (MQ=37)
                                           ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                        <  1:4281870/101‑1 (MQ=25)
                                           ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                        <  1:3058003/101‑1 (MQ=25)
                                            gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                       <  1:2814169/101‑1 (MQ=25)
                                            gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                       <  2:4425031/101‑1 (MQ=25)
                                             ggtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTTGC.CTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATGaaag                                                      <  1:361249/100‑2 (MQ=25)
                                              gtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                     >  2:607453/1‑99 (MQ=25)
                                                gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                   <  2:1861871/101‑5 (MQ=21)
                                                gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                   <  2:3277733/101‑5 (MQ=25)
                                                   aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                               <  2:1804605/101‑1 (MQ=21)
                                                    aCCGGGGCGCTTTGGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                              <  2:188200/101‑1 (MQ=21)
                                                      cGTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGCTGCCCCCCCCC.TTGG.CTTCTGCCAATTCCCACCCCCCGGGATTTTGGGCAAGATGAAAAAACAAAggg                                             >  1:567806/1‑101 (MQ=255)
                                                       gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGtt                                           >  1:2352669/1‑101 (MQ=21)
                                                       gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGtt                                           <  2:1884910/101‑1 (MQ=25)
                                                        tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                          <  1:1837944/101‑1 (MQ=25)
                                                         ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                         <  1:3999301/101‑1 (MQ=25)
                                                          gcgcTTTTGCGCAGGCAAAAGGGGTGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                        <  1:526971/101‑1 (MQ=21)
                                                          gcgcTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                        <  2:2220102/101‑1 (MQ=25)
                                                              ttttGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                    <  2:4416073/101‑1 (MQ=25)
                                                                  gcgcAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                <  2:1669204/101‑1 (MQ=25)
                                                                   cgcAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                               <  2:4058540/101‑1 (MQ=25)
                                                                    cgacccgaaaAGGGGGCGCTG.CCCCCCGC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCaaa                             <  1:77379/94‑1 (MQ=17)
                                                                     cAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaaa                             <  2:1112464/101‑1 (MQ=25)
                                                                      ccccgaaaAGGGGGCTCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAata                           <  1:1989155/96‑1 (MQ=17)
                                                                      aGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                            <  1:4694603/101‑1 (MQ=21)
                                                                       ggCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                           <  2:2394440/101‑1 (MQ=25)
                                                                       ggCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                           <  2:3246101/101‑1 (MQ=25)
                                                                        ccgaaaAGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                         >  2:4203224/4‑101 (MQ=17)
                                                                        ccgaaaAGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                         <  2:2346504/98‑1 (MQ=17)
                                                                         cgaaaAGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATcc                        >  1:3545823/3‑101 (MQ=9)
                                                                         cAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                         <  2:3398727/101‑1 (MQ=25)
                                                                           aaaGGGGGGCGCTC.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGTGGTATTTGGAGCAAGATG.AAAGACTATGGGTTCATCTTGCCTCAAATATccc                       <  1:3142659/101‑1 (MQ=18)
                                                                           aaaAGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                      >  2:1113414/1‑101 (MQ=17)
                                                                           aaaAGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                      <  1:4557588/101‑1 (MQ=17)
                                                                            aaagggggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                     >  2:2693804/4‑101 (MQ=17)
                                                                              ggggggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                    >  1:3464504/1‑101 (MQ=21)
                                                                              agggggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                   >  2:2484948/2‑101 (MQ=18)
                                                                              agggggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                   <  1:2454564/100‑1 (MQ=18)
                                                                               gggggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                  >  2:2963111/1‑101 (MQ=18)
                                                                                 gggCGCTG.CCCCCCTC.TTGGCCTTCGGGCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                 <  1:3107933/101‑1 (MQ=25)
                                                                                 gggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGGTATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                >  1:2514684/1‑99 (MQ=17)
                                                                                 gggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGCGGGATTTGGAGAAAGATGAAAA.ACCATGGGTTCATCTTTCCTCAAATATCCCgggggg                 >  2:4276616/1‑101 (MQ=21)
                                                                                  ggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                <  2:3708193/101‑1 (MQ=25)
                                                                                  ggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                <  2:4654299/101‑1 (MQ=25)
                                                                                   gcgcTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg               <  1:3925227/101‑1 (MQ=25)
                                                                                    cgcTG.CCCCCCTC.TTGGCCTTCGGCCAATTCTCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg             <  2:2803561/101‑6 (MQ=21)
                                                                                    cgcTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGt              <  1:2353004/101‑1 (MQ=25)
                                                                                     ccTG.CCCGCTTA.TTGGCCTTCGGCCAAATCTCCCCCCGGGAAATTTGCGTGAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc            <  2:1188943/100‑7 (MQ=21)
                                                                                       tG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt           <  1:3350898/101‑1 (MQ=25)
                                                                                       tG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt           >  1:3944849/1‑101 (MQ=25)
                                                                                        g.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTTGAGCAAGATG.AAAGACCATGGGGTCATCTTGCCTCAAATATCCCGGGGGGGGTCCTg          >  1:2907301/1‑101 (MQ=25)
                                                                                            ccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa       >  1:3286893/1‑101 (MQ=37)
                                                                                            ccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa       <  2:430373/101‑1 (MQ=37)
                                                                                             cccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg      >  2:3294340/1‑101 (MQ=37)
                                                                                             cccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg      <  1:3399039/101‑1 (MQ=37)
                                                                                             cccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg      <  2:2289244/101‑1 (MQ=37)
                                                                                               ctc.tTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa    >  1:1723413/1‑101 (MQ=37)
                                                                                                tc.tTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc   <  1:1459164/101‑1 (MQ=37)
                                                                                                 c.tTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg  >  1:4085158/1‑101 (MQ=25)
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CCGGTTGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACG  >  NC_014034/2640060‑2640256

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG