BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	279,645	+A	3.8%	intergenic (‑47/‑33)	RCAP_rcc00242 ← / → dapD	hypothetical protein/2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	279,645	0	.→A	3.8%	35.7 ‑0.6 ‑0.0	286	intergenic (‑47/‑33)	RCAP_rcc00242/dapD	hypothetical protein/2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase
Reads supporting (aligned to +/- strand): new base (4/7): ref base (136/139): total (140/146)
Fisher's exact test strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.90e-01

Only 100 of 313 total aligned reads displayed.


TTTTGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAACCCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGCC  >  NC_014034/279545‑279742


 tttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAcc                                                                                                  >  1:458121/1‑101 (MQ=255)


  ttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAcca                                                                                                 >  2:696473/1‑100 (MQ=255)


  ttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAcca                                                                                                 >  2:2904609/1‑100 (MQ=255)


  ttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCCCATTGCGCGAGGGGCCTTCCAACACTGTAGAGGGAAAAcca                                                                                                 >  2:3831435/1‑100 (MQ=255)


   tGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAccat                                                                                                <  1:934626/101‑3 (MQ=255)


   tGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAccat                                                                                                <  2:1270560/101‑3 (MQ=255)


    gCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAccatg                                                                                               <  2:4606605/101‑4 (MQ=255)


     cATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAccatga                                                                                              <  1:2648534/101‑5 (MQ=255)


       tCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAAt                                                                                           <  1:3674392/101‑1 (MQ=255)


           tgatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCa                                                                                       <  1:4414236/101‑1 (MQ=255)


            gatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCAc                                                                                      >  2:3594802/1‑101 (MQ=255)


            gatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCAc                                                                                      >  1:4487411/1‑101 (MQ=255)


              tgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACtt                                                                                    >  1:4911/1‑101 (MQ=255)


               gCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTg                                                                                   >  1:4213189/1‑101 (MQ=255)


               gCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTg                                                                                   <  2:4487161/101‑1 (MQ=255)


               gCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTg                                                                                   <  2:2488789/101‑1 (MQ=255)


                cTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGa                                                                                  >  1:2769857/1‑101 (MQ=255)


                 tccgatctaaCGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGaa                                                                                 <  1:820046/93‑1 (MQ=255)


                 tGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGaa                                                                                 <  2:198854/101‑1 (MQ=255)


                 tGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGaa                                                                                 >  2:3612272/1‑101 (MQ=255)


                  gTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGaaa                                                                                >  1:972711/1‑101 (MQ=255)


                  gTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTAGatc                                                                                >  2:3097005/1‑99 (MQ=255)


                    cACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTTCTCATTGAGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAaga                                                                              >  1:2096477/1‑101 (MQ=255)


                      cGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAgg                                                                            >  1:634460/1‑101 (MQ=255)


                         aaCGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGAcc                                                                         <  1:2496020/101‑1 (MQ=255)


                           cGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGt                                                                       >  2:562206/1‑101 (MQ=255)


                            gggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGtt                                                                      <  1:3595021/101‑1 (MQ=255)


                              gTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTcc                                                                    <  1:4660417/101‑1 (MQ=255)


                               tGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCg                                                                   <  1:1736044/101‑1 (MQ=255)


                                gggAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGa                                                                  <  2:4143015/101‑1 (MQ=255)


                                gggAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGa                                                                  <  1:1038643/101‑1 (MQ=255)


                                  gAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATg                                                                >  1:1444586/1‑101 (MQ=255)


                                  gAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATg                                                                >  1:1332156/1‑101 (MQ=255)


                                   aaCGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGt                                                               <  1:567386/101‑1 (MQ=255)


                                   aaCGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGAACCGTTCCGATgc                                                               >  1:3663338/1‑100 (MQ=255)


                                     cGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCg                                                             >  2:4240351/1‑101 (MQ=255)


                                      ggCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGa                                                            <  2:779187/101‑1 (MQ=255)


                                      ggCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGa                                                            <  2:3244817/101‑1 (MQ=255)


                                        cTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAAc                                                          <  2:1727377/101‑1 (MQ=255)


                                         tttCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACg                                                         >  2:4637023/1‑101 (MQ=255)


                                          ttCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGc                                                        >  1:2272118/1‑101 (MQ=255)


                                           tCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGcc                                                       >  1:4340250/1‑101 (MQ=255)


                                            cGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCa                                                      >  2:3766611/1‑101 (MQ=255)


                                            cGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCa                                                      >  1:2602785/1‑101 (MQ=255)


                                            cGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCa                                                      >  1:3067256/1‑101 (MQ=255)


                                             gTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCAt                                                     >  2:1620170/1‑101 (MQ=255)


                                             gTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCAt                                                     <  2:2789996/101‑1 (MQ=255)


                                              tCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATg                                                    <  1:2951361/101‑1 (MQ=255)


                                                 ttCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTc                                                 >  2:925237/1‑101 (MQ=255)


                                                  tCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCg                                                <  2:3392194/101‑1 (MQ=255)


                                                    atatCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGaa                                              <  2:4061632/101‑1 (MQ=255)


                                                       tCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCt                                           <  2:534485/101‑1 (MQ=255)


                                                        ccTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTg                                          >  2:4229814/1‑101 (MQ=255)


                                                        ccTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTg                                          <  2:1091621/101‑1 (MQ=255)


                                                        ccTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTg                                          <  2:236382/101‑1 (MQ=255)


                                                        ccTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTg                                          <  2:628357/101‑1 (MQ=255)


                                                         cTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGc                                         >  2:1793183/1‑101 (MQ=255)


                                                          tGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGcc                                        <  1:1826278/101‑1 (MQ=255)


                                                           gCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCa                                       >  2:2246197/1‑101 (MQ=255)


                                                             tCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATc                                     <  2:4189824/101‑1 (MQ=255)


                                                             tCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATc                                     <  1:2729483/101‑1 (MQ=255)


                                                              cATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCg                                    <  1:3450478/101‑1 (MQ=255)


                                                              cATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCg                                    <  2:3713963/101‑1 (MQ=255)


                                                               aTTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCGACTTGAAAGAGGACCGTTCCGATGTCGAAAGCCATGCGCGAAGATGCCATCGa                                   >  2:3951214/1‑101 (MQ=255)


                                                                ttGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGaa                                  <  1:532659/101‑1 (MQ=255)


                                                                   ggcgAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgcc                               <  2:1146600/100‑1 (MQ=255)


                                                                   cgcgAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgcc                               >  2:2555005/1‑101 (MQ=255)


                                                                    gcgAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgccg                              <  2:2276401/101‑1 (MQ=255)


                                                                      gAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgccgcc                            >  2:2929980/1‑101 (MQ=255)


                                                                       aGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCt                           <  1:4443704/101‑1 (MQ=255)


                                                                       aGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCt                           <  2:1895910/101‑1 (MQ=255)


                                                                       aGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCt                           <  2:1579358/101‑1 (MQ=255)


                                                                       aGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCt                           <  1:3790580/101‑1 (MQ=255)


                                                                        ggggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTg                          <  1:3950377/101‑1 (MQ=255)


                                                                        ggggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTg                          <  1:2874115/101‑1 (MQ=255)


                                                                         gggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTgg                         >  2:2459144/1‑101 (MQ=255)


                                                                         gggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTgg                         >  1:3834690/1‑101 (MQ=255)


                                                                          ggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTggg                        <  1:4637279/101‑1 (MQ=255)


                                                                           gCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGa                       >  1:1124153/1‑101 (MQ=255)


                                                                           gCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGa                       <  1:3715182/101‑1 (MQ=255)


                                                                            cTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAg                      >  1:1986020/1‑101 (MQ=255)


                                                                             tttCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAgg                     >  1:1536892/1‑101 (MQ=255)


                                                                              ttCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGt                    >  1:3314708/1‑101 (MQ=255)


                                                                               tCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGtt                   <  1:163589/101‑1 (MQ=255)


                                                                               tCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGtt                   <  2:145476/101‑1 (MQ=255)


                                                                                ccccAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTc                  >  2:2043688/1‑101 (MQ=255)


                                                                                ccccAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTc                  <  2:4336466/101‑1 (MQ=255)


                                                                                 cccAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTcg                 <  1:4086039/101‑1 (MQ=255)


                                                                                     aCTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGac             >  2:3010396/1‑101 (MQ=255)


                                                                                       tataGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGacac           >  1:4175443/1‑101 (MQ=255)


                                                                                          aGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCt        >  2:155938/1‑101 (MQ=255)


                                                                                          aGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCt        >  2:947997/1‑101 (MQ=255)


                                                                                           gggggAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTc       <  1:3108965/101‑1 (MQ=255)


                                                                                            ggggAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACAcccaga      >  2:220425/1‑98 (MQ=255)


                                                                                            ggggAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCa      <  1:2246321/101‑1 (MQ=255)


                                                                                             gggAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCAc     <  1:2182740/101‑1 (MQ=255)


                                                                                               ggaaaACCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACg    >  1:4545857/3‑101 (MQ=255)


                                                                                                gaaaACCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGc   <  1:2804026/100‑1 (MQ=255)


                                                                                                gaaaACCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGc   >  1:993325/2‑101 (MQ=255)


                                                                                                 aaaACCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGcc  <  1:719451/101‑1 (MQ=255)


TTTTGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAACCCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGCC  >  NC_014034/279545‑279742

Base quality scores:


ATCG

< 3 ≤


ATCG

< 24 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG