BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,532,987	+G	92.2%	intergenic (‑6/‑19)	RCAP_rcc03340 ← / → RCAP_rcc03341	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,532,987	1	.→G	92.2%	40.4 ‑0.5 ‑0.5	192	intergenic (‑6/‑19)	RCAP_rcc03340/RCAP_rcc03341	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (84/93): ref base (6/9): total (90/102)
Fisher's exact test strand distribution p-value = 6.04e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.52e-01

Only 100 of 205 total aligned reads displayed.


AGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTC.GGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.GGCCC.GCCCTTCGATCCAGTGCAGCCAACCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.CTGCGCGCCCGGCAGGGCCGCCAGATCGTG  >  NC_014034/3532888‑3533079


aGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGc                                                                                                 <  1:4451113/101‑1 (MQ=255)


 gCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.g                                                                                               <  2:2425251/101‑1 (MQ=255)


     ggAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.Gggcc                                                                                           <  1:1811314/101‑1 (MQ=255)


      gAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGGCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.Gggccc                                                                                          <  1:774656/101‑2 (MQ=255)


       aaaGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.Gggcccg                                                                                         >  2:840925/1‑99 (MQ=255)


        aaGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.Gggcccgg                                                                                        >  1:3445665/1‑98 (MQ=255)


             gcgcCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.Gggcccggccctt                                                                                   >  2:1204246/1‑93 (MQ=255)


             gcgcCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.Gggcccggccctt                                                                                   <  2:1120803/101‑9 (MQ=255)


              cgcCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTc                                                                                   >  1:454707/1‑101 (MQ=255)


               gcCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCg                                                                                  <  2:2909620/101‑1 (MQ=255)


                ccTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGa                                                                                 <  2:3764746/101‑1 (MQ=255)


                ccTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGa                                                                                 >  2:4699233/1‑101 (MQ=255)


                 cTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGAt                                                                                <  1:2648422/101‑1 (MQ=255)


                 cTGTCGGCCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGAt                                                                                <  2:3101735/101‑1 (MQ=255)


                  tGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATc                                                                               <  1:3284058/101‑1 (MQ=255)


                    tCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCa                                                                             >  2:2123460/1‑101 (MQ=255)


                     cGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGCTCCAg                                                                            >  2:4106730/1‑101 (MQ=255)


                     cGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAg                                                                            >  2:3652392/1‑101 (MQ=255)


                      ggTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGt                                                                           >  1:2081323/1‑101 (MQ=255)


                      ggTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGt                                                                           >  2:57357/1‑101 (MQ=255)


                       gTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTg                                                                          <  1:2727999/101‑1 (MQ=255)


                         ccAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCa                                                                        <  1:1543121/101‑1 (MQ=255)


                         ccAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCa                                                                        >  1:1875013/1‑101 (MQ=255)


                         ccAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCa                                                                        <  1:4377539/101‑1 (MQ=255)


                          cAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAg                                                                       >  1:4696540/1‑101 (MQ=255)


                            gCCGCCCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGcc                                                                     <  2:1446016/101‑1 (MQ=255)


                             ccgaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCGGCGGGCCCGGCCCTTCGATCCAGTGCAGCCa                                                                    <  2:3582451/101‑1 (MQ=255)


                              cgaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCag                                                                   <  2:2510128/101‑2 (MQ=255)


                              cgaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCag                                                                   <  2:3265025/101‑2 (MQ=255)


                               gaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCTGCCCTTCGATCCAGTGCAGCCagc                                                                  <  2:1039434/101‑3 (MQ=255)


                               gaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCagc                                                                  >  2:1951262/1‑99 (MQ=255)


                               gaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCagc                                                                  >  2:423114/1‑99 (MQ=255)


                               gaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCagc                                                                  >  1:242848/1‑99 (MQ=255)


                                  cgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGt                                                               >  2:4514235/1‑101 (MQ=255)


                                   gaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTc                                                              >  2:3136711/1‑101 (MQ=255)


                                    aaaGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTcc                                                             <  1:3042241/101‑1 (MQ=255)


                                      agcagcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTc                                                           <  2:279676/101‑1 (MQ=255)


                                      agcagcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTc                                                           <  2:2997976/101‑1 (MQ=255)


                                      agcagcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTc                                                           <  2:723431/101‑1 (MQ=255)


                                      agcagcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTc                                                           <  1:1246949/101‑1 (MQ=255)


                                       gcagcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCg                                                          <  2:420368/101‑1 (MQ=255)


                                        cagcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGa                                                         <  1:1673469/101‑1 (MQ=255)


                                         agcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGAc                                                        >  2:1734929/1‑101 (MQ=255)


                                         agcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGAc                                                        <  2:1650012/101‑1 (MQ=255)


                                         agcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGAc                                                        >  2:4280884/1‑101 (MQ=255)


                                           ccGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCg                                                      <  1:4312181/101‑1 (MQ=255)


                                           ccGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCg                                                      <  2:3445458/101‑1 (MQ=255)


                                              cgcCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCaa                                                   <  2:3060412/101‑1 (MQ=255)


                                              cgcCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCaa                                                   <  1:216179/101‑1 (MQ=255)


                                              cgcCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCaa                                                   <  1:935759/101‑1 (MQ=255)


                                               gcCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAc                                                  <  1:1469726/101‑1 (MQ=255)


                                                ccTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAcc                                                 <  1:1954637/101‑1 (MQ=255)


                                                 cTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCg                                                <  1:57364/101‑1 (MQ=255)


                                                  tCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGt                                               <  1:3801355/101‑1 (MQ=255)


                                                   cGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTc                                              <  1:2367403/101‑1 (MQ=255)


                                                    ggCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCg                                             <  2:3128341/101‑1 (MQ=255)


                                                    ggCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCg                                             <  1:3940663/101‑1 (MQ=255)


                                                    ggCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCg                                             <  1:423158/101‑1 (MQ=255)


                                                     gCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGc                                            >  2:623946/1‑101 (MQ=255)


                                                      ccAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGcc                                           <  2:482010/101‑1 (MQ=255)


                                                       cAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGccc                                          <  2:1476314/101‑1 (MQ=255)


                                                       cAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGccc                                          >  1:2520838/1‑101 (MQ=255)


                                                        aGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGcccc                                         <  1:2910131/101‑1 (MQ=255)


                                                           cGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGca                                      >  2:45750/1‑101 (MQ=255)


                                                            gggggTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGcac                                     <  2:3112997/101‑1 (MQ=255)


                                                              gggTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAg                                   <  1:1046215/101‑1 (MQ=255)


                                                               ggtcgggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGa                                  >  1:4328969/6‑101 (MQ=255)


                                                                gtcgggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAt                                 <  2:3696137/97‑1 (MQ=255)


                                                                gtcgggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAt                                 >  2:1001855/5‑101 (MQ=255)


                                                                 tcgggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.c                               <  1:2132180/98‑1 (MQ=255)


                                                                 gcgggggCGTGCTCCGCCGTCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.c                               <  2:1275864/98‑1 (MQ=255)


                                                                   gggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                             >  1:3509215/2‑99 (MQ=255)


                                                                   gggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                             >  2:259504/2‑99 (MQ=255)


                                                                    ggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctg                            <  1:2267481/101‑4 (MQ=255)


                                                                    ggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctg                            >  2:278204/1‑98 (MQ=255)


                                                                     gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctgc                           <  1:314216/101‑5 (MQ=255)


                                                                     gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctgc                           <  1:734462/101‑5 (MQ=255)


                                                                         gATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTgcgcgc                        >  1:3733956/1‑101 (MQ=255)


                                                                          aTCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGcc                       >  1:2850007/1‑101 (MQ=255)


                                                                          aTCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGcc                       >  1:3683683/1‑101 (MQ=255)


                                                                            cTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCg                     <  2:1166071/101‑1 (MQ=255)


                                                                            cTGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCg                     >  2:4349881/1‑101 (MQ=255)


                                                                             tGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCgg                    >  1:2347945/1‑101 (MQ=255)


                                                                             tGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCgg                    >  1:2926994/1‑101 (MQ=255)


                                                                             tGCGCCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCgg                    <  2:2889750/101‑1 (MQ=255)


                                                                              gcgcCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGc                   <  2:4388020/101‑1 (MQ=255)


                                                                                gcCATCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAg                 >  1:3827681/1‑101 (MQ=255)


                                                                                   aTCGCGGCGCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgc              >  2:353715/1‑101 (MQ=255)


                                                                                     cgcgGCGCAAGGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgccg            >  1:4104136/1‑101 (MQ=255)


                                                                                       cggcgCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgccgcc          <  2:3673014/101‑1 (MQ=255)


                                                                                       cggcgCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgccgcc          <  2:2081202/101‑1 (MQ=255)


                                                                                        ggcgCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgccgccc         >  1:899050/1‑100 (MQ=255)


                                                                                        ggcgCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCa         >  2:3321912/1‑101 (MQ=255)


                                                                                        ggcgCAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCa         <  1:1379274/101‑1 (MQ=255)


                                                                                          tctaaaGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGa       <  1:3122240/98‑1 (MQ=255)


                                                                                            cAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATc     >  2:2264080/1‑101 (MQ=255)


                                                                                            cAAAGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGACGTCCTCGACCGCACCCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATc     >  2:1919070/1‑101 (MQ=255)


                                                                                             aaaGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATCg    <  2:2472087/101‑1 (MQ=255)


                                                                                              aaGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATCGt   <  1:144627/101‑1 (MQ=255)


                                                                                               aGCTGCGGGCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATCGTg  <  2:271903/101‑1 (MQ=255)


AGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTC.GGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.GGCCC.GCCCTTCGATCCAGTGCAGCCAACCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.CTGCGCGCCCGGCAGGGCCGCCAGATCGTG  >  NC_014034/3532888‑3533079

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG