Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	774,641	+A	7.1%	intergenic (‑95/+25)	oppB1 ← / ← oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	774,641	1	.→A	7.1%	2.4 ‑2.6 0.0	28	intergenic (‑95/+25)	oppB1/oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (1/1):  ref base (26/0):  total (27/1)
Fisher's exact test strand distribution p-value = 7.14e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

CCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCCGGT.C.GA.CG.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACGC  >  NC_014034/774600‑774730                                            |                                                                                            gcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCa                                  >  1:585880/6‑101 (MQ=255)    cggcgcCCCCCCGCCCGGGTCGGACCGGCCCCGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCCGTCGGCCACGTTCCAGTa                               >  2:4697769/3‑101 (MQ=255)       cgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCCATCACTTCGCCCGCGACCAGTCAGCCACGTTCCAGTATTc                            >  2:4263997/1‑101 (MQ=255)                   cgggtcggaCCGGCCCTGCCGGTCC.GACCC.TCCGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGc                >  1:397252/9‑101 (MQ=255)                   cgggtcggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGCATTCGCTGTCGCAGGc                >  2:4488293/9‑101 (MQ=255)                   cgggtcggaCCGGCCCTGCCGGT.CAGA.CCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGc                >  2:4230560/9‑101 (MQ=255)                    gggtcggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTCTTCGCTGTCCCAGGCg               >  1:1622236/8‑101 (MQ=255)                     ggtcggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGt              >  2:975293/7‑101 (MQ=255)                     ggtcggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCAGCCACGTTCCAGTATTCGCTGTCCCAGGCGt              >  2:1279058/7‑101 (MQ=255)                       tcggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTc            >  2:477297/5‑101 (MQ=255)                        cggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCTTGCGTTCACTTCTCCCGCGACCAGTCGGCCACGTTCCAGTATTCGATGTCCCAGGCGTTCa           >  1:3912497/4‑101 (MQ=255)                        cggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa           >  1:1880321/4‑101 (MQ=255)                        cggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa           >  1:2505871/4‑101 (MQ=255)                        cggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa           >  1:3100470/4‑101 (MQ=255)                        cggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa           >  2:1061265/4‑101 (MQ=255)                         ggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt          >  2:157641/3‑101 (MQ=255)                          gaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtc         >  1:784488/2‑101 (MQ=255)                          gaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtc         >  1:4723792/2‑101 (MQ=255)                               gCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc    >  2:3904684/1‑101 (MQ=255)                               gCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc    >  1:3760342/1‑101 (MQ=255)                               gCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc    >  1:4402848/1‑101 (MQ=255)                               ccccTGCCGGT.CAGACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc    <  1:4230807/100‑1 (MQ=255)                                cccTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACg   >  2:1248577/1‑101 (MQ=255)                                cccTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACg   >  2:4218159/1‑101 (MQ=255)                                cccTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACg   >  1:3583558/1‑101 (MQ=255)                                cccTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACg   >  1:2817913/1‑101 (MQ=255)                                 ccTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACGc  >  2:1830114/1‑101 (MQ=255)                                 ccTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCACAGGCGTTCATCTCGACGc  >  2:4190492/1‑101 (MQ=255)                                 ccTGCCGGTCC.GA.CCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACGc  >  2:1696554/1‑101 (MQ=255)                                            |                                                                                            CCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCCGGT.C.GA.CG.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACGC  >  NC_014034/774600‑774730 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG