New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_014034	= 1397797	98 (0.530)	35 (0.190)	5/156	25.6	0.42	intergenic (+101/+1797)	RCAP_rcc01307/RCAP_rcc01309	hypothetical protein/IS66 family transposase
	?	NC_014034	1434665 =	NA (NA)					intergenic (‑444/‑514)	RCAP_rcc01334/RCAP_rcc01335	hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Rejected: Position hash score below cutoff.

AGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....................................  >  NC_014034/1397698‑1397797 ..........................................................................................................................GGGACAGGCTTTGC  >  NC_014034/1434665‑1434678                                                                                                     ||||||||||||||||||||||               atGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT                                     <  2:2367337/99‑1 (MQ=1) AGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT                                     >  1:655936/1‑101 (MQ=1)  GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTTAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTC                                    <  2:2424071/101‑1 (MQ=0)  GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTC                                    <  2:1140981/101‑1 (MQ=1)  GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTC                                    >  1:2783944/1‑101 (MQ=1)  GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTC                                    <  2:861632/101‑1 (MQ=1)  GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTC                                    >  2:2985675/1‑101 (MQ=1)   GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCG                                   <  2:4741133/101‑1 (MQ=1)    TCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGG                                  <  1:4623389/101‑1 (MQ=1)    TCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGG                                  >  2:4362805/1‑101 (MQ=0)    TCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGCATCGAAACTCGG                                  <  1:2578839/101‑1 (MQ=0)     CGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGC                                 >  2:3457832/1‑101 (MQ=1)      GCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCA                                >  2:44552/1‑101 (MQ=1)      GCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCA                                <  2:872298/101‑1 (MQ=1)       CTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAA                               >  2:4398255/1‑101 (MQ=1)       CTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAA                               >  1:928039/1‑101 (MQ=1)       CTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAA                               <  1:3213203/101‑1 (MQ=1)        TCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG                              >  1:859271/1‑101 (MQ=1)        TCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG                              <  1:3571695/101‑1 (MQ=1)        TCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG                              >  2:1758057/1‑101 (MQ=1)        TCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG                              <  2:1448611/101‑1 (MQ=1)         CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGG                             <  1:3783118/101‑1 (MQ=1)         CCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGG                             <  1:3974306/101‑1 (MQ=1)          CCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGA                            <  1:335965/101‑1 (MQ=1)           CCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGAC                           >  1:3138297/1‑101 (MQ=1)           CCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGAC                           <  1:1586760/101‑1 (MQ=1)           CCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGAC                           <  2:3832343/101‑1 (MQ=1)           CCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGAC                           <  1:4520794/101‑1 (MQ=1)           CCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAGTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGAC                           <  2:4284421/101‑1 (MQ=0)             CCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT                         <  1:523110/101‑1 (MQ=1)             CCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT                         >  1:516475/1‑101 (MQ=1)             CCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT                         <  1:4439531/101‑1 (MQ=1)             CCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT                         <  1:3191231/101‑1 (MQ=1)               GCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCG                       <  2:2920413/101‑1 (MQ=1)               GCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCG                       <  1:3275980/101‑1 (MQ=1)                gGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGC                      >  1:2476762/2‑101 (MQ=1)                CGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGAGATCGC                      >  1:399627/1‑101 (MQ=0)                CGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGC                      <  2:3205469/101‑1 (MQ=1)                CGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGC                      >  1:2399586/1‑101 (MQ=1)                CGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGC                      <  1:3046532/101‑1 (MQ=1)                 GGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCC                     >  1:4436458/1‑101 (MQ=1)                 GGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCC                     <  1:3458044/101‑1 (MQ=1)                 GGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCC                     <  1:1578423/101‑1 (MQ=1)                 GGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCC                     <  2:3970256/101‑1 (MQ=0)                    gGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGAT                  <  1:3681332/100‑1 (MQ=1)                     GCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATC                 >  1:418505/1‑101 (MQ=1)                      CGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACCTCGCCGATCT                >  2:3770434/1‑101 (MQ=1)                      CGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCT                <  1:3426020/101‑1 (MQ=1)                      CGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCACTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCT                <  2:2235723/101‑1 (MQ=1)                       GTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTG               >  2:3630978/1‑101 (MQ=1)                       GTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTG               >  2:968756/1‑101 (MQ=1)                        TGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGG              >  1:1335782/1‑101 (MQ=1)                        TGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGG              <  1:3086517/101‑1 (MQ=1)                         GGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGG             >  1:741483/1‑101 (MQ=1)                         GGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGG             <  2:2625650/101‑1 (MQ=1)                         GGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGG             >  1:3905142/1‑101 (MQ=1)                         GGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGG             <  1:3638452/101‑1 (MQ=1)                         GGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGG             >  1:3212707/1‑101 (MQ=1)                         GGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACTCGGCAAGGACATCGCCGATCTGGG             >  1:3313891/1‑101 (MQ=1)                           ATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGta           <  2:927962/101‑3 (MQ=1)                           ATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGta           <  1:1478758/101‑3 (MQ=1)                           ATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAAGACATCGCCGATCTGGGta           >  2:4722173/1‑99 (MQ=1)                            TCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGtaa          <  1:4560840/101‑4 (MQ=1)                            TCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGtaa          <  2:2437683/101‑4 (MQ=1)                             CGAACCTTGTCTGACGCNCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGtaag         <  2:399583/101‑5 (MQ=1)                             CGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGtaag         >  1:116680/1‑97 (MQ=1)                             CGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGtaag         <  1:2436871/101‑5 (MQ=1)                             CGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGtaag         <  1:4398502/101‑5 (MQ=1)                             CGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGtaag         >  1:4662566/1‑97 (MQ=1)                              GAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGtaagg        >  2:1498725/1‑96 (MQ=1)                              GAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGtaagg        >  1:2744753/1‑96 (MQ=1)                              GAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGtaagg        >  1:1432501/1‑96 (MQ=1)                              GAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGtaagg        >  1:708527/1‑96 (MQ=1)                              GAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGtaagg        >  1:1618762/1‑96 (MQ=1)                               AAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGC       >  1:1429608/1‑101 (MQ=1)                               AAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGC       >  2:694546/1‑101 (MQ=1)                                AACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCc      <  2:1432407/101‑2 (MQ=1)                                AACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCc      >  2:3186736/1‑100 (MQ=1)                                AACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCc      <  2:3292592/101‑2 (MQ=1)                                 ACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCcg     >  1:2476182/1‑99 (MQ=1)                                 ACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCcg     >  2:3758900/1‑99 (MQ=1)                                    TGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCcggtg  <  2:305718/101‑6 (MQ=1)                                    TGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCcggtg  <  1:2767391/101‑6 (MQ=1)                                    TGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCGCCGATCTGGGTAAGGCcggtg  >  2:2061649/1‑96 (MQ=1)                                                                                                     ||||||||||||||||||||||               AGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC....................................  >  NC_014034/1397698‑1397797 ..........................................................................................................................GGGACAGGCTTTGC  >  NC_014034/1434665‑1434678 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG