Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,185,9870T→G100.0% 2.7 4intergenic (+260/‑245)mtlK/RCAP_rcc02028mannitol 2‑dehydrogenase/FeoA family protein
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/3):  ref base (0/1):  total (0/4)

ACCGCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGG  >  NC_014034/2185927‑2186073
                                                              |                                                                                      
ccgcccccTTTCACCTTGGCTAAAATATCCGGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCac                                                <  2:3631859/97‑1 (MQ=2)
               ttGGCTCAAATATCCCGGGGGTCGGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGAc                                 <  2:589166/101‑1 (MQ=9)
                tGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGGCT.CGGGCGCAGGGCGGGGGCG.GAGAACCCAGCGCCCCccaccgacccgcggcc                                >  2:457866/1‑93 (MQ=2)
                                  ggTCGGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGcc              <  2:4492861/101‑1 (MQ=12)
                                     ccGGGGGCGGAGCCCCCGGCGGGGCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCCGCGCCCCCGACCGACCTGCGACGGCCCGTCGCCCAAAAGCCCgg           >  2:2558113/1‑101 (MQ=9)
                                           ggtgcccccccGGCGGGTCTCCGCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggc     <  2:1484840/93‑1 (MQ=255)
                                              gcgcccccGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggcagg  <  2:216517/96‑1 (MQ=21)
                                                              |                                                                                      
ACCGCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGG  >  NC_014034/2185927‑2186073

Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG