Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,427 A→C 5.7% intergenic (+239/‑692) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4270A→C 5.7% 22.2 ‑1.5 ‑0.4194intergenic (+239/‑692)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/7):  ref base (102/81):  total (106/88)
Fisher's exact test strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.64e-01

Only 100 of 247 total aligned reads displayed.

CAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1395327‑1395527
                                                                                                      |                                                                                                    
cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.a                                                                                                      >  2:1888783/1‑101 (MQ=255)
cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.a                                                                                                      >  1:1431060/1‑101 (MQ=255)
cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.a                                                                                                      <  1:2718479/101‑1 (MQ=255)
cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.a                                                                                                      >  2:4648326/1‑101 (MQ=255)
cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcgggg.a                                                                                                      >  1:1240469/1‑101 (MQ=255)
  aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggtgatcggtg.atc                                                                                                    >  2:4142524/1‑101 (MQ=255)
    tttccccGGCTGGTCCGGGGGTTTCGGAACGGGAGTCGCCCCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                                                   <  1:122525/96‑1 (MQ=25)
    ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                                  >  1:1576605/1‑101 (MQ=255)
    ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                                  <  1:3222068/101‑1 (MQ=255)
    ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                                  >  2:4028171/1‑101 (MQ=255)
      acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGGG.ATCAGCg                                                                                                >  2:2692648/1‑101 (MQ=255)
       cacCGTCTGGTGAGGGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGGGGATCGAAGCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCgg                                                                                               <  2:3269012/101‑1 (MQ=38)
         ccGTCTGGTCAGCGTGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTc                                                                                             <  2:3118557/101‑1 (MQ=255)
         ccGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTc                                                                                             <  1:499883/101‑1 (MQ=255)
         ccGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGGG.ATCAGCGGTc                                                                                             >  2:2507284/1‑101 (MQ=255)
          cGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCg                                                                                            >  2:1526228/1‑101 (MQ=255)
           gTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                           <  2:901034/101‑1 (MQ=255)
           gTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                           >  1:1542723/1‑101 (MQ=255)
           gTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGGGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                           >  1:1128238/1‑101 (MQ=255)
            tCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCt                                                                                          <  1:2950590/101‑1 (MQ=255)
             cTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTc                                                                                         >  2:2158305/1‑101 (MQ=255)
              tGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcc                                                                                        >  2:241564/1‑101 (MQ=255)
               ggTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccc                                                                                       <  1:3285908/101‑1 (MQ=255)
                gTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcccc                                                                                      >  2:859224/1‑101 (MQ=255)
                 tCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccccc                                                                                     <  1:1565066/101‑1 (MQ=255)
                   aGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcg                                                                                   >  1:4362631/1‑101 (MQ=255)
                      gggTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgg                                                                                >  1:946309/1‑101 (MQ=255)
                        gTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgggg                                                                              <  2:3851517/101‑1 (MQ=255)
                        gTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgggg                                                                              >  2:2603266/1‑101 (MQ=255)
                         ttACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCggggg                                                                             >  2:3899445/1‑101 (MQ=255)
                          tACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGc                                                                            >  2:4307418/1‑101 (MQ=255)
                           aCGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCg                                                                           >  2:1658087/1‑101 (MQ=255)
                             gAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                                                         <  1:1745134/101‑1 (MQ=255)
                             gAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                                                         <  1:3805763/101‑1 (MQ=255)
                              tcaacactgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      >  1:1142741/9‑101 (MQ=2)
                              aaaCGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGGGATCGGGG.ATCAGCGGGCGCTCCCCCCGCGGGGGCGTgg                                                                        >  1:1392815/1‑101 (MQ=255)
                               aaCGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                       <  2:4427497/101‑1 (MQ=255)
                                aCGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGGGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGaa                                                                      >  2:839720/1‑100 (MQ=255)
                                 acactgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                   >  2:2395214/6‑101 (MQ=2)
                                     tgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                               <  2:364363/100‑2 (MQ=2)
                                     aGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGGGTGGAGCGaaa                                                                 >  1:2930439/1‑101 (MQ=255)
                                     aGTCG.CCCCCCCCGCGGGGGCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 <  1:403347/101‑1 (MQ=255)
                                       tCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                               >  1:3556467/1‑101 (MQ=255)
                                       tCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                               >  1:1952128/1‑101 (MQ=255)
                                        cG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                              >  1:2278449/1‑101 (MQ=255)
                                        cG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                              >  1:678747/1‑101 (MQ=255)
                                         g.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                             >  2:4056848/1‑101 (MQ=255)
                                            tCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgc                                                           >  1:1799584/1‑101 (MQ=255)
                                            tCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgc                                                           >  2:4343137/1‑101 (MQ=255)
                                            cccccccGCGGGGGCGTNGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgc                                                           <  2:1542622/100‑1 (MQ=255)
                                              cccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgccg                                                         <  1:2376186/101‑1 (MQ=255)
                                               ccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgccgc                                                        <  1:1042531/101‑1 (MQ=255)
                                                  cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCttt                                                     <  1:4162914/101‑1 (MQ=255)
                                                  cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCttt                                                     <  2:4602113/101‑1 (MQ=255)
                                                   gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTg                                                    <  1:2125642/101‑1 (MQ=255)
                                                     gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                  >  2:295191/1‑101 (MQ=255)
                                                     gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                  >  2:2547667/1‑101 (MQ=255)
                                                       gggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCgg                                                <  2:3446393/101‑1 (MQ=255)
                                                        ggCGTGTATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                                               <  2:3491787/101‑1 (MQ=255)
                                                        ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                                               >  1:3108544/1‑101 (MQ=255)
                                                        ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                                               >  1:1904689/1‑101 (MQ=255)
                                                         gCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTc                                              >  2:292233/1‑101 (MQ=255)
                                                            tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                           >  2:4190470/1‑101 (MQ=255)
                                                            tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                           <  2:1169461/101‑1 (MQ=255)
                                                              gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                         >  1:4496414/1‑101 (MQ=255)
                                                               aTCGAATCTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCTTGGATCGAAACATCGCCGCTTTGGCGGGCGACAgg                                        <  2:2891306/101‑1 (MQ=38)
                                                               aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAgg                                        >  1:1601440/1‑101 (MQ=255)
                                                                 cGAAACTGCCCCCGCGGGCCCTGCCGGTGATCGGTG.ACCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                                      <  1:3304601/101‑1 (MQ=255)
                                                                  gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                                     >  1:2466069/1‑101 (MQ=255)
                                                                   aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                    <  2:4250736/101‑1 (MQ=255)
                                                                   aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                    <  2:3598960/101‑1 (MQ=255)
                                                                   aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                    <  1:261324/101‑1 (MQ=255)
                                                                   aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                    <  2:805090/101‑1 (MQ=255)
                                                                    aaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt                                   >  2:2451969/1‑101 (MQ=255)
                                                                     aCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                                  >  1:87160/1‑101 (MQ=255)
                                                                      cTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTaa                                 <  2:2835698/101‑1 (MQ=255)
                                                                       tGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAAc                                >  1:4724104/1‑101 (MQ=255)
                                                                        gCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                               >  2:472824/1‑101 (MQ=255)
                                                                        gCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                               >  2:100228/1‑101 (MQ=255)
                                                                         cACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgt                              >  1:3903611/1‑101 (MQ=255)
                                                                              gCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGc                         >  1:3366984/1‑101 (MQ=255)
                                                                               cTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAACGTGTCGCt                        >  2:4518900/1‑101 (MQ=255)
                                                                                tGGCCCTGCCGGTGATCGGTA.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                       <  2:119426/101‑1 (MQ=255)
                                                                                 ggCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                      >  2:3203681/1‑101 (MQ=255)
                                                                                  gCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                     <  1:79447/101‑1 (MQ=255)
                                                                                  gCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                     <  1:4690757/101‑1 (MQ=255)
                                                                                   cccttagggtgatcggtg.aagGGCGGGCGCCCGCCGCGCGGGGGCGTGGATCGAGACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc                    <  2:1973446/94‑1 (MQ=255)
                                                                                     cTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                  <  2:1240379/101‑1 (MQ=255)
                                                                                      tGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGCAACGTGTCGCTCCCCCcg                 >  1:1706350/1‑101 (MQ=255)
                                                                                         cggtgatcggtg.atCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgg              >  2:2184999/1‑101 (MQ=255)
                                                                                          ggtgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggg             <  2:2025776/101‑1 (MQ=255)
                                                                                           gtgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg            <  1:3381087/101‑1 (MQ=255)
                                                                                             gatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc          <  1:3692393/101‑1 (MQ=255)
                                                                                             gatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc          >  1:1896893/1‑101 (MQ=255)
                                                                                             gatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc          >  1:1805842/1‑101 (MQ=255)
                                                                                                 ggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg      <  1:3721657/101‑1 (MQ=255)
                                                                                                 ggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg      <  2:568243/101‑1 (MQ=255)
                                                                                                    g.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATc   <  2:3764451/101‑1 (MQ=255)
                                                                                                      atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGGTCg  >  2:2088856/1‑101 (MQ=255)
                                                                                                      atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  2:1801971/101‑1 (MQ=255)
                                                                                                      |                                                                                                    
CAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1395327‑1395527

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG