Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,157,144 G→A 9.8% A56T (GCC→ACC)  dam → adenine‑specific DNA‑methyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,157,1440G→A 9.8% 48.5 ‑1.0 ‑0.4218A56T (GCC→ACC) damadenine‑specific DNA‑methyltransferase
Reads supporting (aligned to +/- strand):  new base (12/9):  ref base (86/108):  total (99/119)
Fisher's exact test strand distribution p-value = 3.57e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.34e-01

Only 100 of 236 total aligned reads displayed.

ACGGATCTGCACGATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCTGCGCTTCCAGCTGAC  >  NC_014034/2157044‑2157244
                                                                                                    |                                                                                                    
  ggATCTGCACGATCCTCGACGAAACCCCCTGCCCGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGcc                                                                                                    >  2:2787597/1‑101 (MQ=255)
   gATCTGCACGATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCg                                                                                                   <  2:1239622/101‑1 (MQ=255)
   gATCTGCACGATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCg                                                                                                   <  2:1791311/101‑1 (MQ=255)
   gATCTGCACGATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCg                                                                                                   >  2:3804551/1‑101 (MQ=255)
    aTCTGCACGATCCTCGACGAACCCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGa                                                                                                  <  2:3551328/101‑1 (MQ=255)
    aTCTCCACGATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGACCgg                                                                                                  >  1:4472667/1‑100 (MQ=255)
     tccgcccGATCCTCGACGAAACCCCCTGCCGGCCCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAg                                                                                                 <  2:3021855/95‑1 (MQ=255)
     tCTGCACGATCCTCGACGAACCCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAg                                                                                                 <  2:869808/101‑1 (MQ=255)
     tCTGCACGATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAg                                                                                                 >  1:1444742/1‑101 (MQ=255)
        gCACGATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTg                                                                                              >  2:2676432/1‑101 (MQ=255)
        gCACGATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTg                                                                                              >  1:2115134/1‑101 (MQ=255)
         cACGATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGa                                                                                             >  1:3750590/1‑101 (MQ=255)
         cACGATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGGGa                                                                                             >  2:236302/1‑101 (MQ=255)
          aCGATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGAt                                                                                            >  1:2678435/1‑101 (MQ=255)
           cGATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATc                                                                                           <  1:410246/101‑1 (MQ=255)
           cGATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATc                                                                                           <  2:527408/101‑1 (MQ=255)
            gATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCa                                                                                          <  1:2018153/101‑1 (MQ=255)
            gATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCa                                                                                          <  1:4645453/101‑1 (MQ=255)
            gATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCa                                                                                          <  1:1559753/101‑1 (MQ=255)
            gATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCa                                                                                          >  2:1952781/1‑101 (MQ=255)
            cgtCCCCGACCAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCa                                                                                          <  1:357619/99‑1 (MQ=255)
             aTCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCaa                                                                                         >  1:868499/1‑101 (MQ=255)
             aTCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGACCGAGGTGATCaa                                                                                         >  1:4738654/1‑101 (MQ=255)
              tCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAat                                                                                        >  1:2476605/1‑101 (MQ=255)
              tCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAat                                                                                        <  1:676098/101‑1 (MQ=255)
                 tCGACGAAACCCCCTGCCGTACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCTGCCGCGCCCGCCCGCGGGCCGAGGAGATCAatgat                                                                                     >  2:4236907/1‑101 (MQ=255)
                 tCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAatgat                                                                                     <  2:1417509/101‑1 (MQ=255)
                    acgaAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCg                                                                                  <  1:120128/101‑1 (MQ=255)
                     cgaAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCgg                                                                                 >  2:4433631/1‑101 (MQ=255)
                       aaaCCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGGGATCAATGATGCGGGc                                                                               >  1:4026248/1‑101 (MQ=255)
                         aCCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCcg                                                                             <  2:3417436/101‑1 (MQ=255)
                              cTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACa                                                                        >  2:1118384/1‑101 (MQ=255)
                                gCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATc                                                                      >  1:432423/1‑101 (MQ=255)
                                gCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATc                                                                      <  2:2434464/101‑1 (MQ=255)
                                gCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATAAATGATGCGGGCCGCGACATc                                                                      >  1:3876457/1‑101 (MQ=255)
                                   ggACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATcgcg                                                                   <  2:1051778/101‑1 (MQ=255)
                                    gACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGa                                                                  >  2:1270495/1‑101 (MQ=255)
                                      ccTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAAc                                                                >  2:2225307/1‑101 (MQ=255)
                                       cTATTGCGAGCCCTTCGTGGGCATGGGCGGGATGTCCCTGCCCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAAcc                                                               <  1:4073825/101‑1 (MQ=255)
                                       cTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAAcc                                                               >  1:3800324/1‑101 (MQ=255)
                                       cTATGGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGACCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAAcc                                                               <  2:4074085/101‑1 (MQ=255)
                                         aTTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTg                                                             >  1:2976082/1‑101 (MQ=255)
                                          ttGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGt                                                            >  2:4329555/1‑101 (MQ=255)
                                          ttGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGt                                                            <  2:2768938/101‑1 (MQ=255)
                                          ttGCGACCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGt                                                            <  2:1444648/101‑1 (MQ=255)
                                           tGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGtt                                                           <  1:3167586/101‑1 (MQ=255)
                                             cGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTcc                                                         >  2:211487/1‑101 (MQ=255)
                                                gCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGa                                                      >  2:1479595/1‑101 (MQ=255)
                                                 cccTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGACCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGAt                                                     >  1:2609724/1‑101 (MQ=255)
                                                  ccTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATc                                                    <  1:1548433/101‑1 (MQ=255)
                                                   cTTCGTGGGCGTGGGCGGGCTCTCCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATcc                                                   <  2:722381/101‑1 (MQ=255)
                                                   cTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATcc                                                   <  1:2380503/101‑1 (MQ=255)
                                                   cTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATcc                                                   <  1:1977002/101‑1 (MQ=255)
                                                    ttCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCt                                                  >  1:2267895/1‑101 (MQ=255)
                                                    ttCGTGGGCATGGGCGGGATCTCCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCt                                                  <  2:4156677/101‑1 (MQ=255)
                                                      cGTGGGCAGGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGc                                                <  2:2009225/101‑1 (MQ=255)
                                                       gTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCa                                               <  2:1519204/101‑1 (MQ=255)
                                                         gggCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGc                                             >  1:731159/1‑101 (MQ=255)
                                                         gggCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGc                                             >  1:2409656/1‑101 (MQ=255)
                                                         gggCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGc                                             >  1:2660919/1‑101 (MQ=255)
                                                            caggggCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGACCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGc                                          <  2:3783288/98‑1 (MQ=255)
                                                            cATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGc                                          <  2:3885580/101‑1 (MQ=255)
                                                            cATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGc                                          >  1:3503597/1‑101 (MQ=255)
                                                            cATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGc                                          <  1:824519/101‑1 (MQ=255)
                                                               gggCGGGCTCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCAtt                                       <  2:119241/101‑1 (MQ=255)
                                                               gggCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCAtt                                       <  1:3082365/101‑1 (MQ=255)
                                                               gggCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCAtt                                       <  1:1720426/101‑1 (MQ=255)
                                                               gggCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCAtt                                       <  1:1678069/101‑1 (MQ=255)
                                                                  cGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATc                                    <  1:3956778/101‑1 (MQ=255)
                                                                  cGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATc                                    >  2:3993100/1‑101 (MQ=255)
                                                                   gggATCTTCCTGCGCCGCCGCGCCCGCCCGCGGACCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATcc                                   >  1:1300242/1‑101 (MQ=255)
                                                                     gATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGc                                 <  2:25730/101‑1 (MQ=255)
                                                                       tCTCCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATGAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAg                               <  2:1710170/101‑1 (MQ=255)
                                                                        cTTCCTGCGCCGCCGCGCCCGCCCGCGGACCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGt                              >  1:4274829/1‑101 (MQ=255)
                                                                         ttCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGtt                             >  1:4618709/1‑101 (MQ=255)
                                                                            cTGCCCCGCCGCGCCCGCCCGCGGACCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCt                          <  2:4600477/101‑1 (MQ=255)
                                                                              gcgcCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCg                        <  2:2595918/101‑1 (MQ=255)
                                                                              gcgcCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCg                        >  1:1761129/1‑101 (MQ=255)
                                                                              gccccgccgcGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCg                        <  1:1644557/98‑1 (MQ=255)
                                                                              gccccgccgcGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCg                        <  1:972878/98‑1 (MQ=255)
                                                                               cgccgccgcGCCCGCCCGCGGACCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTTCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGa                       >  2:3104851/1‑101 (MQ=255)
                                                                                gccgccgcGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGaa                      <  1:1010659/101‑1 (MQ=255)
                                                                                gccgccgcGCCCGCCCGCGGACCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGaa                      <  2:1300150/101‑1 (MQ=255)
                                                                                  cgccgcGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAAt                    <  2:1345489/101‑1 (MQ=255)
                                                                                   gccgcGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATc                   <  2:2163786/101‑1 (MQ=255)
                                                                                    ccgcGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATcc                  <  1:3682568/101‑1 (MQ=255)
                                                                                     cgcgCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCt                 <  1:3397294/101‑1 (MQ=255)
                                                                                      gcgcCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCTg                >  1:3554335/1‑101 (MQ=255)
                                                                                       cgcccgccCGCGGACCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCTgc               <  2:2609572/101‑1 (MQ=255)
                                                                                        gcccgcccGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCTgcg              >  2:2175971/1‑101 (MQ=255)
                                                                                        gcccgcccGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCTgcg              >  2:2842877/1‑101 (MQ=255)
                                                                                          ccgcccgcGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCTGCGCt            <  1:4395382/101‑1 (MQ=255)
                                                                                           cgcccgcGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCTGCGCtt           <  1:4183960/101‑1 (MQ=255)
                                                                                             cccgcGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCTGCGCTTcc         <  1:2296437/101‑1 (MQ=255)
                                                                                             cccgcGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCTGCGCTTcc         <  1:1099355/101‑1 (MQ=255)
                                                                                              ccgcGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCTGCGCTTCCa        <  2:2038977/101‑1 (MQ=255)
                                                                                               cgcgGACCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCTGCGCTTCCAg       >  2:3370859/1‑101 (MQ=255)
                                                                                                gcgGACCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCTGCGCTTCCAGc      >  1:2725381/1‑101 (MQ=255)
                                                                                                 cgGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCTGCGCTTCCAGCt     >  1:2805652/1‑101 (MQ=255)
                                                                                                    gCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCTGCGCTTCCAGCTGAc  >  1:1814414/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
ACGGATCTGCACGATCCTCGACGAAACCCCCTGCCGGACCTATTGCGAGCCCTTCGTGGGCATGGGCGGGATCTTCCTGCGCCGCCGCGCCCGCCCGCGGGCCGAGGTGATCAATGATGCGGGCCGCGACATCGCGAACCTGTTCCGGATCCTGCAGCGGCATTATCCGCAGTTCCTCGAAATCCTGCGCTTCCAGCTGAC  >  NC_014034/2157044‑2157244

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG