Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,185,995	G→C	100%	intergenic (+268/‑237)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,185,995	0	G→C	100.0%	10.1	5	intergenic (+268/‑237)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (0/5):  ref base (0/0):  total (0/5)

ACCGCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGG  >  NC_014034/2185927‑2186073                                                                       |                                                                               ccgcccccTTTCACCTTGGCTAAAATATCCGGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCac                                                <  2:3631859/97‑1 (MQ=2)                ttGGCTCAAATATCCCGGGGGTCGGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGAc                                 <  2:589166/101‑1 (MQ=9)                 tGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGGCT.CGGGCGCAGGGCGGGGGCG.GAGAACCCAGCGCCCCccaccgacccgcggcc                                >  2:457866/1‑93 (MQ=2)                                   ggTCGGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGcc              <  2:4492861/101‑1 (MQ=12)                                      ccGGGGGCGGAGCCCCCGGCGGGGCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCCGCGCCCCCGACCGACCTGCGACGGCCCGTCGCCCAAAAGCCCgg           >  2:2558113/1‑101 (MQ=9)                                            ggtgcccccccGGCGGGTCTCCGCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggc     <  2:1484840/93‑1 (MQ=255)                                               gcgcccccGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggcagg  <  2:216517/96‑1 (MQ=21)                                                                       |                                                                               ACCGCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGG  >  NC_014034/2185927‑2186073 Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG