BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,254,851	C→G	8.3%	E5D (GAG→GAC)	RCAP_rcc02086 ←	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,254,851	0	C→G	8.3%	35.8 ‑1.9 ‑0.0	226	E5D (GAG→GAC)	RCAP_rcc02086	hypothetical protein
Reads supporting (aligned to +/- strand): new base (14/5): ref base (111/96): total (125/101)
Fisher's exact test strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.43e-01

Only 100 of 240 total aligned reads displayed.


TTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCCGGCGGGCACAGATGCTGCTGT  >  NC_014034/2254751‑2254951


tttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAATACGTTTCGCCCCCAGCTTTCGGCGCGGGAtc                                                                                                      >  1:3838214/1‑101 (MQ=255)


tttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtc                                                                                                      >  2:2605774/1‑101 (MQ=255)


tttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtc                                                                                                      >  1:435644/1‑101 (MQ=255)


  tCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtctc                                                                                                    >  1:3927568/1‑101 (MQ=255)


   ccccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTcc                                                                                                   >  2:1240848/1‑101 (MQ=255)


    cccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCca                                                                                                  <  1:1764213/101‑1 (MQ=255)


    cccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCca                                                                                                  >  1:4121045/1‑101 (MQ=255)


     tcgatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgag                                                                                                 >  2:1631838/10‑101 (MQ=255)


     ccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcag                                                                                                 <  2:1750368/101‑1 (MQ=255)


      cgatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgagg                                                                                                >  2:2107987/9‑100 (MQ=255)


      cgatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgagg                                                                                                >  1:2536426/9‑100 (MQ=255)


      cTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagc                                                                                                <  2:282317/101‑1 (MQ=255)


       ttCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagca                                                                                               >  1:2734655/1‑101 (MQ=255)


       ttCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagca                                                                                               >  1:3976847/1‑101 (MQ=255)


       ttCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagca                                                                                               >  1:1549229/1‑101 (MQ=255)


       ttCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagca                                                                                               >  2:1777728/1‑101 (MQ=255)


       gatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggc                                                                                               >  2:3418802/8‑99 (MQ=255)


        atcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggcc                                                                                              <  1:28449/95‑4 (MQ=255)


        atcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggcc                                                                                              <  2:2872708/95‑4 (MQ=255)


          gCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTc                                                                                            <  2:4504938/101‑1 (MQ=255)


          cgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgc                                                                                            >  1:2546755/5‑96 (MQ=255)


           ctctCGGCCTAAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTACAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCg                                                                                           <  2:3570194/101‑1 (MQ=255)


            tctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGc                                                                                          >  1:2247372/1‑101 (MQ=255)


             gtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgcgca                                                                                         >  2:3754563/2‑93 (MQ=255)


             ctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCa                                                                                         >  1:709557/1‑101 (MQ=255)


              tcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCaa                                                                                        <  1:3753305/101‑1 (MQ=255)


               cGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAg                                                                                       <  2:2429046/101‑1 (MQ=255)


                gCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAgg                                                                                      >  1:3275525/1‑101 (MQ=255)


                 cccTCAATGGGCAGCGCCGAGGGGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGt                                                                                     <  1:1160171/101‑1 (MQ=255)


                  ccTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTg                                                                                    <  2:907841/101‑1 (MQ=255)


                   cTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGc                                                                                   >  1:3877342/1‑101 (MQ=255)


                    tCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCt                                                                                  >  2:3443712/1‑101 (MQ=255)


                     cAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCtt                                                                                 <  2:212621/101‑1 (MQ=255)


                     cAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCtt                                                                                 >  1:621855/1‑101 (MQ=255)


                      aaTGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCttt                                                                                >  2:4087517/1‑101 (MQ=255)


                        tGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCa                                                                              <  1:2584024/101‑1 (MQ=255)


                         gggCGGCGCCGAGGCGAAGGCGTCCAGGGCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGGTCTCCAGCAGTCGCAAGGTGCTTTCag                                                                             <  2:2978734/101‑1 (MQ=255)


                         gggCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCag                                                                             >  2:488469/1‑101 (MQ=255)


                         gggCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCag                                                                             <  1:1950333/101‑1 (MQ=255)


                             aGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGAc                                                                         <  1:3063915/101‑1 (MQ=255)


                             aGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGAc                                                                         >  2:1008357/1‑101 (MQ=255)


                                    aGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGAGGCCAAgg                                                                  >  1:4610250/1‑101 (MQ=255)


                                     ggCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGt                                                                 <  2:1549128/101‑1 (MQ=255)


                                      gCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTa                                                                <  2:3275324/101‑1 (MQ=255)


                                       cGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTaa                                                               >  1:544460/1‑101 (MQ=255)


                                        gAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAt                                                              <  1:4328451/101‑1 (MQ=255)


                                        gAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAt                                                              <  1:1287936/101‑1 (MQ=255)


                                              gTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGcgccg                                                        <  2:537312/101‑1 (MQ=255)


                                               tCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGcgccgc                                                       >  2:2072664/1‑101 (MQ=255)


                                                cAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCgccgcc                                                      >  2:4691149/1‑101 (MQ=255)


                                                cAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCgccgcc                                                      >  1:1239472/1‑101 (MQ=255)


                                                 aaGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCt                                                     >  2:3678536/1‑101 (MQ=255)


                                                 aaGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCt                                                     <  1:2470656/101‑1 (MQ=255)


                                                  aGGTCCAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTc                                                    <  2:2271970/101‑1 (MQ=255)


                                                   ggTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCg                                                   <  2:3877100/101‑1 (MQ=255)


                                                   ggTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCg                                                   >  1:2663100/1‑101 (MQ=255)


                                                   ggTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTAGCAAGGCGCTTTCAGAGACGCCAAGGCAATGAGCCGCCTCg                                                   >  1:1456898/1‑101 (MQ=255)


                                                      cGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgc                                                >  2:4541261/1‑101 (MQ=255)


                                                       gAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcg                                               >  2:1324334/1‑101 (MQ=255)


                                                       gAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcg                                               >  2:690051/1‑101 (MQ=255)


                                                       gAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGgcg                                               >  2:2538529/1‑101 (MQ=255)


                                                        aaGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTTAGAGACGCCAAGGTAATGCGCCGCCTCGGgcgc                                              >  1:4075901/1‑101 (MQ=255)


                                                            ggTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGtt                                          <  1:1919232/101‑1 (MQ=255)


                                                            ggTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGtt                                          <  1:2184641/101‑1 (MQ=255)


                                                             gTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTtg                                         <  2:3836113/101‑1 (MQ=255)


                                                             gTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTtg                                         <  1:2446285/101‑1 (MQ=255)


                                                                gTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGCATCGCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGa                                      <  1:4064665/101‑1 (MQ=255)


                                                                 tAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGAt                                     <  1:3794777/101‑1 (MQ=255)


                                                                 tAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGAt                                     >  1:3565659/1‑101 (MQ=255)


                                                                 tAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGAt                                     <  1:4532041/101‑1 (MQ=255)


                                                                 tAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGAt                                     >  1:770010/1‑101 (MQ=255)


                                                                 tAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGAt                                     >  1:974937/1‑101 (MQ=255)


                                                                  aaaGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATc                                    >  1:1580928/1‑101 (MQ=255)


                                                                   aaGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATcc                                   <  2:3067642/101‑1 (MQ=255)


                                                                       cGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGgcg                               <  2:307569/101‑1 (MQ=255)


                                                                         tttCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGcc                             >  2:305477/1‑101 (MQ=255)


                                                                          ttCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCa                            <  1:2072785/101‑1 (MQ=255)


                                                                           tCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAg                           <  1:1782769/101‑1 (MQ=255)


                                                                           tCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAg                           >  2:3158499/1‑101 (MQ=255)


                                                                           tCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAg                           >  2:1900235/1‑101 (MQ=255)


                                                                            cGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAgg                          >  2:2459588/1‑101 (MQ=255)


                                                                             gCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGt                         >  1:853800/1‑101 (MQ=255)


                                                                              cccccAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTc                        >  1:1304699/1‑101 (MQ=255)


                                                                                cccAGCTTTCGGCGGGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCcg                      <  1:3025089/101‑1 (MQ=255)


                                                                                cccAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCcg                      <  2:4562439/101‑1 (MQ=255)


                                                                                 ccAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGTTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCcgg                     <  2:2265539/101‑1 (MQ=255)


                                                                                    gCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCcggcgg                  >  2:2728398/1‑101 (MQ=255)


                                                                                      tttCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCCGGCGGGc                >  2:1364376/1‑101 (MQ=255)


                                                                                       ttCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCCGGCGGGca               >  2:1929363/1‑101 (MQ=255)


                                                                                         cGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCCGGCGGGcaca             <  1:3443919/101‑1 (MQ=255)


                                                                                          ggCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCCGGCGGGCACAg            >  2:4132651/1‑101 (MQ=255)


                                                                                           gcgcGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCCGGCGGGCACAGa           <  2:434509/101‑1 (MQ=255)


                                                                                             gcgGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCCGGCGGGCACAGAtg         >  1:1118914/1‑101 (MQ=255)


                                                                                               gggCTCTCACGCCGTAGCAAGGTGCTTTCAGAGACACCGAGGTAATGCGCCGCCTCGGGCCCGGTTGCCTTCCGGCGCCAGGACCGGCGGGCACAGAtgct       >  2:4615070/1‑101 (MQ=255)


                                                                                                 gATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCCGGCGGGCACAGAtgctgc     <  2:1664919/101‑1 (MQ=255)


                                                                                                 gATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCACCAGGTCCGGCGGGCACAGAtgctgc     >  1:3990543/1‑101 (MQ=255)


                                                                                                  aTCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCCGGCGGGCACAGATgctgct    >  2:4242753/1‑101 (MQ=255)


                                                                                                    ctcCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCCGGCGGGCACAGATGCTGCTGt  <  2:432808/101‑1 (MQ=255)


                                                                                                    ctcCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCCGGCGGGCACAGATGCTGCTGt  >  1:4415899/1‑101 (MQ=255)


                                                                                                    ctcCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCCGGCGGGCACAGATGCTGCTGt  >  2:959494/1‑101 (MQ=255)


TTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAATGCGCCGCCTCGGGCGCGGTTGTGATCCGGCGCCAGGTCCGGCGGGCACAGATGCTGCTGT  >  NC_014034/2254751‑2254951

Base quality scores:


ATCG

< 3 ≤


ATCG

< 24 ≤


ATCG

< 31 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG