Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,191 G→C 16.0% intergenic (‑167/+109) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1910G→C16.0% 62.3 ‑1.0 ‑0.0156intergenic (‑167/+109)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (13/12):  ref base (55/76):  total (68/88)
Fisher's exact test strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.62e-01

Only 100 of 169 total aligned reads displayed.

ACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640092‑2640291
                                                                                                      |                                                                                                    
aCGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTgg                                                                                                      <  1:218594/101‑1 (MQ=255)
   cggcggCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGagc                                                                                                   <  1:3961578/101‑3 (MQ=255)
    ggcgggAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCa                                                                                                  <  2:3254120/101‑1 (MQ=25)
     gcggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCaa                                                                                                 <  1:2778093/101‑1 (MQ=25)
      gggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAg                                                                                                <  2:3368113/100‑1 (MQ=37)
      cggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGACGGATTTGGAGCAAg                                                                                                >  2:2352871/1‑101 (MQ=25)
       ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                                                               <  2:3240426/101‑1 (MQ=37)
       ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                                                               <  2:2917760/101‑1 (MQ=37)
       ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                                                               <  2:3924693/101‑1 (MQ=37)
         cAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                                                             <  1:3683988/101‑1 (MQ=25)
           ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                           <  1:4281870/101‑1 (MQ=25)
           ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                           <  1:3058003/101‑1 (MQ=25)
            gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                          <  1:2814169/101‑1 (MQ=25)
            gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                          <  2:2911592/101‑1 (MQ=25)
             ggtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTTGC.CTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATGaaag                                                                                         <  1:361249/100‑2 (MQ=25)
              gtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                                                        >  2:607453/1‑99 (MQ=25)
                gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                                                      <  2:1861871/101‑5 (MQ=21)
                   aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                  <  2:1804605/101‑1 (MQ=21)
                    aCCGGGGCGCTTTGGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                 <  2:188200/101‑1 (MQ=21)
                      cGTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGCTGCCCCCCCCC.TTGG.CTTCTGCCAATTCCCACCCCCCGGGATTTTGGGCAAGATGAAAAAACAAAggg                                                                                >  1:567806/1‑101 (MQ=255)
                       gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGtt                                                                              >  1:2352669/1‑101 (MQ=21)
                       ctGGTGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGtt                                                                              <  1:1475094/100‑1 (MQ=21)
                              ttttGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                       <  2:4416073/101‑1 (MQ=25)
                                  gcgcAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                   <  2:1669204/101‑1 (MQ=25)
                                    cgacccgaaaAGGGGGCGCTG.CCCCCCGC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCaaa                                                                <  1:77379/94‑1 (MQ=17)
                                     gccccgaaaAGGGGGCGCTG.CCCCCCTC.TTGGTCTTCGGGCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                               <  1:3862236/95‑1 (MQ=9)
                                      ccccgaaaAGGGGGCTCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAata                                                              <  1:1989155/96‑1 (MQ=17)
                                      aGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                                                               <  2:2473578/101‑1 (MQ=25)
                                       ggCAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                              <  2:2394440/101‑1 (MQ=25)
                                        ccgaaaAGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                                                            <  2:2346504/98‑1 (MQ=17)
                                         cAAAAGGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                            <  2:3398727/101‑1 (MQ=25)
                                           aaaAGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                         <  1:3545633/101‑1 (MQ=17)
                                           aaaAGGGGGCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                         >  2:1113414/1‑101 (MQ=17)
                                            aaagggggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                        >  2:2693804/4‑101 (MQ=17)
                                            aaagggggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                        >  2:490676/4‑101 (MQ=17)
                                              ggggggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                       >  1:3464504/1‑101 (MQ=21)
                                              agggggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                      >  1:4582159/2‑101 (MQ=18)
                                                ggggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggg                                                    >  1:920075/1‑101 (MQ=18)
                                                 gggCGCTG.CCCCCCTC.TTGGCCTTCGGGCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                    <  1:3107933/101‑1 (MQ=25)
                                                 gggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGGTATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                   >  1:2514684/1‑99 (MQ=17)
                                                 gggCGCTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGCGGGATTTGGAGAAAGATGAAAA.ACCATGGGTTCATCTTTCCTCAAATATCCCgggggg                                                    >  2:4276616/1‑101 (MQ=21)
                                                   gcgcTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                 <  2:2658265/101‑5 (MQ=21)
                                                   gcgcTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                                  <  1:3925227/101‑1 (MQ=25)
                                                    cgcTG.CCCCCCTC.TTGGCCTTCGGCCAATTCTCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                <  2:2803561/101‑6 (MQ=21)
                                                    cgcTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                <  1:1167898/101‑6 (MQ=21)
                                                     ccTG.CCCGCTTA.TTGGCCTTCGGCCAAATCTCCCCCCGGGAAATTTGCGTGAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                               <  2:1188943/100‑7 (MQ=21)
                                                      cTG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                               <  1:1923838/101‑1 (MQ=25)
                                                       tG.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                              <  1:3350898/101‑1 (MQ=25)
                                                        g.CCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTTGAGCAAGATG.AAAGACCATGGGGTCATCTTGCCTCAAATATCCCGGGGGGGGTCCTg                                             >  1:2907301/1‑101 (MQ=25)
                                                            ccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                          <  2:430373/101‑1 (MQ=37)
                                                            ccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                          >  1:3286893/1‑101 (MQ=37)
                                                             cccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                         <  2:2289244/101‑1 (MQ=37)
                                                             cccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                         <  1:3399039/101‑1 (MQ=37)
                                                             cccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                         >  2:3294340/1‑101 (MQ=37)
                                                               ctc.tTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                       >  1:1723413/1‑101 (MQ=37)
                                                                 c.tTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                     >  1:4085158/1‑101 (MQ=25)
                                                                 c.tTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                     <  1:1567318/101‑1 (MQ=37)
                                                                     ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                  >  1:2561448/4‑101 (MQ=37)
                                                                           cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                            <  2:3464292/101‑1 (MQ=255)
                                                                           cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGAGGGCaga                            >  2:4177219/1‑101 (MQ=37)
                                                                             gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                          <  1:4382493/101‑1 (MQ=255)
                                                                              ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                         <  2:3257122/101‑1 (MQ=255)
                                                                               cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGAGGGGGCCCTGAAAGGACGGGGGGCAGAGccc                        >  2:629234/1‑101 (MQ=25)
                                                                                aaTTCACCCCCCGAGGTATTTGGGGCAAGATG.AAAGACCGTGGGATCATCTTGCCTCAAATATCGCGCCGGGAGTCCTGAAAGGAGGGGGGGCACAGcgcc                       >  2:3818712/1‑101 (MQ=19)
                                                                                aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                       >  1:2444517/1‑101 (MQ=37)
                                                                                aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                       >  2:1247573/1‑101 (MQ=255)
                                                                                 aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                      <  2:1989199/101‑1 (MQ=255)
                                                                                 aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                      >  1:1362666/1‑101 (MQ=255)
                                                                                 aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                      >  1:4674788/1‑101 (MQ=255)
                                                                                 aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCACGGGGGCAGTCCTGATAGGACGGGGGACAGACccccc                      >  2:1900101/1‑101 (MQ=25)
                                                                                  ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGTGGCAGAGcccccc                     >  2:2976755/1‑101 (MQ=25)
                                                                                   tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCACAGCCCCCCt                    >  2:459148/4‑101 (MQ=25)
                                                                                    caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   >  2:203479/3‑101 (MQ=255)
                                                                                       cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                <  2:2421040/101‑1 (MQ=255)
                                                                                       cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                <  1:4135344/101‑1 (MQ=255)
                                                                                       cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                >  2:2975898/1‑101 (MQ=255)
                                                                                        ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg               >  1:4513128/1‑101 (MQ=255)
                                                                                         cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgag              >  1:665174/1‑99 (MQ=255)
                                                                                          ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCGGAGCCCCCCTCCCGGGt             >  1:20988/1‑101 (MQ=37)
                                                                                           cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc            >  1:4041019/1‑101 (MQ=255)
                                                                                            gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           >  1:137667/1‑101 (MQ=255)
                                                                                             aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          >  1:2350304/1‑101 (MQ=255)
                                                                                             aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          >  2:1393269/1‑101 (MQ=255)
                                                                                              ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt         <  2:1766876/101‑1 (MQ=255)
                                                                                               gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc        >  2:1123499/1‑101 (MQ=255)
                                                                                                tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct       >  2:2754765/1‑101 (MQ=255)
                                                                                                tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct       >  1:3176031/1‑101 (MQ=255)
                                                                                                tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct       >  1:88993/1‑101 (MQ=255)
                                                                                                 aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc      >  2:705936/1‑101 (MQ=255)
                                                                                                 aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc      >  1:3761020/1‑101 (MQ=255)
                                                                                                  tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa     <  2:3686629/101‑1 (MQ=255)
                                                                                                  tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa     >  1:4720246/1‑101 (MQ=255)
                                                                                                  tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa     <  1:1042070/101‑1 (MQ=255)
                                                                                                   ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa    >  2:809106/1‑101 (MQ=255)
                                                                                                    tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg   >  1:3185223/1‑101 (MQ=255)
                                                                                                     ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  2:4621315/1‑101 (MQ=255)
                                                                                                     ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  2:3010764/1‑101 (MQ=255)
                                                                                                     ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  2:1055013/1‑101 (MQ=255)
                                                                                                     ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  1:694005/1‑101 (MQ=255)
                                                                                                     ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  <  1:1041480/101‑1 (MQ=255)
                                                                                                      |                                                                                                    
ACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640092‑2640291

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG