Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,210 T→G 13.8% intergenic (‑186/+90) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2100T→G13.8% 67.5 ‑0.2 ‑0.0181intergenic (‑186/+90)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (13/12):  ref base (87/69):  total (100/81)
Fisher's exact test strand distribution p-value = 8.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.57e-01

Only 100 of 189 total aligned reads displayed.

AACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGA  >  NC_014034/2640111‑2640310
                                                                                                     |                                                                                                    
aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                                     <  2:1804605/101‑1 (MQ=21)
 aCCGGGGCGCTTTGGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                                    <  2:188200/101‑1 (MQ=21)
   cGTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGCTGCCCCCCCCCTTGG.CTTCTGCCAATTCCCACCCCCCGGGATTTTGGGCAAGATGAAAAAACAAAggg                                                                                                   >  1:567806/1‑101 (MQ=255)
    gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGtt                                                                                                 >  1:2352669/1‑101 (MQ=21)
     tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                                <  1:4109557/101‑1 (MQ=25)
     tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                                <  1:1837944/101‑1 (MQ=25)
      ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                                                                               <  1:3999301/101‑1 (MQ=25)
       gcgcTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                                              <  2:2220102/101‑1 (MQ=25)
                  gccccgaaaAGGGGGCGCTG.CCCCCCTCTTGGTCTTCGGGCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                                                  <  1:3862236/95‑1 (MQ=9)
                    ggCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                                                 <  2:2394440/101‑1 (MQ=25)
                    ggCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                                                 <  2:3246101/101‑1 (MQ=25)
                     ccgaaaAGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                                                                               <  2:2346504/98‑1 (MQ=17)
                      cgaaaAGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATcc                                                                              >  1:3545823/3‑101 (MQ=9)
                      cAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                                               <  2:3398727/101‑1 (MQ=25)
                       caaaGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATcc                                                                              <  2:2358587/100‑1 (MQ=21)
                        aaaGGGGGGCGCTC.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGTGGTATTTGGAGCAAGATG.AAAGACTATGGGTTCATCTTGCCTCAAATATccc                                                                             <  1:3142659/101‑1 (MQ=18)
                        aaaAGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                            >  2:1113414/1‑101 (MQ=17)
                        aaaAGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                            >  1:3071039/1‑101 (MQ=17)
                         aaagggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                                           >  2:2693804/4‑101 (MQ=17)
                         aaagggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                                           >  2:490676/4‑101 (MQ=17)
                           agggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                                         >  1:4582159/2‑101 (MQ=18)
                           agggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                                         <  1:2454564/100‑1 (MQ=18)
                           agggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                                         >  2:2484948/2‑101 (MQ=18)
                             ggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggg                                                                       >  1:920075/1‑101 (MQ=18)
                             ggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggggg                                                                        <  1:4196744/101‑1 (MQ=25)
                              gggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                                      <  1:2542500/101‑3 (MQ=18)
                              gggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGCGGGATTTGGAGAAAGATGAAAA.ACCATGGGTTCATCTTTCCTCAAATATCCCgggggg                                                                       >  2:4276616/1‑101 (MQ=21)
                              gggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCCCGGCATTTTGAGCAAGATG.AAAGACCATGGGGTCATCTTTCCTCAAATATCCCcggggg                                                                       >  2:1127926/1‑101 (MQ=16)
                               ggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                                      <  2:4654299/101‑1 (MQ=25)
                                gcgcTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                                                     <  1:3925227/101‑1 (MQ=25)
                                 cgcTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                                   <  1:2825796/101‑6 (MQ=21)
                                 cgcTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                                   <  1:1167898/101‑6 (MQ=21)
                                   cTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                                  <  1:1923838/101‑1 (MQ=25)
                                    tG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                                 <  1:607517/101‑1 (MQ=25)
                                        cccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                              <  2:3327975/101‑1 (MQ=37)
                                          cccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                            <  1:3399039/101‑1 (MQ=37)
                                            ctctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                                          >  1:1723413/1‑101 (MQ=37)
                                             tctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                         <  1:1459164/101‑1 (MQ=37)
                                              ctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                                        <  1:1567318/101‑1 (MQ=37)
                                                tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                      >  1:4490279/5‑101 (MQ=37)
                                                tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                      >  2:4381375/5‑101 (MQ=37)
                                                tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                      >  1:726826/5‑101 (MQ=37)
                                                    cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                                  >  2:2452919/1‑101 (MQ=37)
                                                       cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                               >  1:2789889/1‑101 (MQ=37)
                                                       cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                               <  2:3464292/101‑1 (MQ=255)
                                                       cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGAGGGCaga                                               >  2:4177219/1‑101 (MQ=37)
                                                         gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                             <  1:4382493/101‑1 (MQ=255)
                                                           cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGAGGGGGCCCTGAAAGGACGGGGGGCAGAGccc                                           >  2:629234/1‑101 (MQ=25)
                                                            aaTTCACCCCCCGAGGTATTTGGGGCAAGATG.AAAGACCGTGGGATCATCTTGCCTCAAATATCGCGCCGGGAGTCCTGAAAGGAGGGGGGGCACAGcgcc                                          >  2:3818712/1‑101 (MQ=19)
                                                            aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                          >  2:2727196/1‑101 (MQ=37)
                                                            aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                          >  2:2778012/1‑101 (MQ=255)
                                                             aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                         >  1:4674788/1‑101 (MQ=255)
                                                             aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                         >  2:16762/1‑101 (MQ=37)
                                                             aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                         >  2:1213101/1‑101 (MQ=37)
                                                             aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCACGGGGGCAGTCCTGATAGGACGGGGGACAGACccccc                                         >  2:1900101/1‑101 (MQ=25)
                                                              ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGTGGCAGAGcccccc                                        >  2:2976755/1‑101 (MQ=25)
                                                               tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCACAGCCCCCCt                                       >  2:459148/4‑101 (MQ=25)
                                                                caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                      >  2:203479/3‑101 (MQ=255)
                                                                   cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                                   >  2:2975898/1‑101 (MQ=255)
                                                                    ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                                  <  2:1454458/101‑1 (MQ=255)
                                                                     cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg                                 >  1:3533448/1‑101 (MQ=255)
                                                                      ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCGGAGCCCCCCTCCCGGGt                                >  1:20988/1‑101 (MQ=37)
                                                                        gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                              >  1:137667/1‑101 (MQ=255)
                                                                          ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt                            <  2:1766876/101‑1 (MQ=255)
                                                                           gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc                           >  1:875311/1‑101 (MQ=255)
                                                                             aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                         >  2:705936/1‑101 (MQ=255)
                                                                              tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa                        >  1:4720246/1‑101 (MQ=255)
                                                                              tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa                        <  2:3686629/101‑1 (MQ=255)
                                                                               ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                       <  2:2871811/101‑1 (MQ=255)
                                                                               ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                       >  2:809106/1‑101 (MQ=255)
                                                                                tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg                      >  1:3185223/1‑101 (MQ=255)
                                                                                 ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                     <  1:1041480/101‑1 (MQ=255)
                                                                                 ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                     >  2:1055013/1‑101 (MQ=255)
                                                                                 ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                     >  1:694005/1‑101 (MQ=255)
                                                                                    gcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGtt                  <  2:3548818/100‑1 (MQ=255)
                                                                                     cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                 >  2:2001548/1‑101 (MQ=255)
                                                                                     cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                 >  1:1841831/1‑101 (MQ=255)
                                                                                       aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg               >  1:1029815/1‑101 (MQ=255)
                                                                                       aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg               <  1:3297876/101‑1 (MQ=255)
                                                                                          gtgaaaGACCATGGGTTCATCTTGCCGCAAAGATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc             <  2:1289827/98‑1 (MQ=255)
                                                                                           tgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCt            >  2:3048687/3‑101 (MQ=255)
                                                                                            gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc           <  2:2075615/100‑1 (MQ=255)
                                                                                            gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc           <  1:1077600/100‑1 (MQ=255)
                                                                                            gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc           >  1:2171405/2‑101 (MQ=255)
                                                                                            gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc           >  2:3882507/2‑101 (MQ=255)
                                                                                            gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGAAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGGTCCGACTc           >  2:2522902/2‑101 (MQ=255)
                                                                                             aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa          >  1:3768364/1‑101 (MQ=255)
                                                                                             aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCCGCTcg          >  2:4252361/1‑100 (MQ=255)
                                                                                              aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg         >  1:7473/1‑101 (MQ=255)
                                                                                              aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCCTTTCTCAAGCGTTCCGGCTCAg         >  1:1293108/1‑101 (MQ=255)
                                                                                               aGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCTCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGa        >  1:4051063/1‑101 (MQ=255)
                                                                                                gACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa       <  1:2976942/101‑1 (MQ=255)
                                                                                                gACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa       >  2:2600141/1‑101 (MQ=255)
                                                                                                gACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa       >  2:737620/1‑101 (MQ=255)
                                                                                                 aCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc      <  2:3286691/101‑1 (MQ=255)
                                                                                                 aCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc      >  2:1546798/1‑101 (MQ=255)
                                                                                                 aCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGCCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGACTTCCTCAAGCGTTCCGGCTCAGAAc      >  1:2393147/1‑101 (MQ=255)
                                                                                                  ccATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAcc     >  1:3593976/1‑101 (MQ=255)
                                                                                                    aTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCgg   >  1:4541274/1‑101 (MQ=255)
                                                                                                    aTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAAGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCgg   >  2:4102579/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
AACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGA  >  NC_014034/2640111‑2640310

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG