Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,216 C→T 13.0% intergenic (‑192/+84) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2160C→T13.0% 59.3 ‑1.0 ‑0.0186intergenic (‑192/+84)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (12/12):  ref base (96/65):  total (109/77)
Fisher's exact test strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.95e-01

Only 100 of 195 total aligned reads displayed.

TGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCG  >  NC_014034/2640116‑2640316
                                                                                                     |                                                                                                    
  gcgcTTTTGCGCAGGCAAAAGGGGTGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                                                    <  1:526971/101‑1 (MQ=21)
             cAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaaa                                                                                         <  2:1112464/101‑1 (MQ=25)
              ccccgaaaAGGGGGCTCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAata                                                                                       <  1:1989155/96‑1 (MQ=17)
              aGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                                                                                        <  1:4694603/101‑1 (MQ=21)
               ggCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                                                       <  2:2394440/101‑1 (MQ=25)
               ggCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                                                       <  2:3246101/101‑1 (MQ=25)
                ccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                                                                                     >  2:4203224/4‑101 (MQ=17)
                ccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                                                                                     <  2:2346504/98‑1 (MQ=17)
                 cgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATcc                                                                                    >  1:3545823/3‑101 (MQ=9)
                 cAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                                                     <  2:3398727/101‑1 (MQ=25)
                  caaaGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATcc                                                                                    <  2:2358587/100‑1 (MQ=21)
                    aaagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                                                 >  2:490676/4‑101 (MQ=17)
                     aagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                                                >  2:187717/3‑101 (MQ=18)
                      agggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                                               >  1:4582159/2‑101 (MQ=18)
                      agggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                                               <  1:2454564/100‑1 (MQ=18)
                      agggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                                               >  2:2484948/2‑101 (MQ=18)
                         gggCGCTGCCCCCCTCTTGGCCTTCGGGCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                                             <  1:3107933/101‑1 (MQ=25)
                         gggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                                            <  1:2542500/101‑3 (MQ=18)
                         gggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGCGGGATTTGGAGAAAGATGAAAA.ACCATGGGTTCATCTTTCCTCAAATATCCCgggggg                                                                             >  2:4276616/1‑101 (MQ=21)
                          ggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                                            <  2:4654299/101‑1 (MQ=25)
                           gcgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                                          <  2:2658265/101‑5 (MQ=21)
                            cgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                                         <  1:2825796/101‑6 (MQ=21)
                             ccTGCCCGCTTATTGGCCTTCGGCCAAATCTCCCCCCGGGAAATTTGCGTGAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                                                        <  2:1188943/100‑7 (MQ=21)
                               tGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                                       <  1:607517/101‑1 (MQ=25)
                               tGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                                       >  1:3944849/1‑101 (MQ=25)
                               tGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                                       <  1:3350898/101‑1 (MQ=25)
                                   ccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                                                   >  1:3286893/1‑101 (MQ=37)
                                    cccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                                  <  1:3399039/101‑1 (MQ=37)
                                    cccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                                  >  2:3294340/1‑101 (MQ=37)
                                       tctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                               <  1:1459164/101‑1 (MQ=37)
                                        ctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                                              >  1:4085158/1‑101 (MQ=25)
                                          tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                            >  1:726826/5‑101 (MQ=37)
                                          tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                            >  2:4381375/5‑101 (MQ=37)
                                          tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                            >  1:4490279/5‑101 (MQ=37)
                                              cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                                        >  2:2452919/1‑101 (MQ=37)
                                                 cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                                     <  2:3464292/101‑1 (MQ=255)
                                                 cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                                     >  1:2789889/1‑101 (MQ=37)
                                                   gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                                   <  1:4382493/101‑1 (MQ=255)
                                                     cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                                                 <  2:1048494/101‑1 (MQ=255)
                                                     cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGAGGGGGCCCTGAAAGGACGGGGGGCAGAGccc                                                 >  2:629234/1‑101 (MQ=25)
                                                      aaTTCACCCCCCGAGGTATTTGGGGCAAGATG.AAAGACCGTGGGATCATCTTGCCTCAAATATCGCGCCGGGAGTCCTGAAAGGAGGGGGGGCACAGcgcc                                                >  2:3818712/1‑101 (MQ=19)
                                                      aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                                >  2:1247573/1‑101 (MQ=255)
                                                      aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                                >  2:2727196/1‑101 (MQ=37)
                                                      aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                                >  2:2778012/1‑101 (MQ=255)
                                                       aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                               <  2:1989199/101‑1 (MQ=255)
                                                       aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                               >  2:16762/1‑101 (MQ=37)
                                                        ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGTGGCAGAGcccccc                                              >  2:2976755/1‑101 (MQ=25)
                                                         tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCACAGCCCCCCt                                             >  2:459148/4‑101 (MQ=25)
                                                          caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                            >  2:203479/3‑101 (MQ=255)
                                                           accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                           >  1:1145054/2‑101 (MQ=255)
                                                           accccccGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTGCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                           <  1:2169463/100‑1 (MQ=25)
                                                             cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                                         >  2:2975898/1‑101 (MQ=255)
                                                             cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                                         <  1:4135344/101‑1 (MQ=255)
                                                               cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg                                       >  1:3533448/1‑101 (MQ=255)
                                                                 cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                                     >  1:4041019/1‑101 (MQ=255)
                                                                  gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                                    <  1:1247663/101‑1 (MQ=255)
                                                                  gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                                    >  1:137667/1‑101 (MQ=255)
                                                                   aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                                   >  2:1393269/1‑101 (MQ=255)
                                                                   aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                                   >  1:2350304/1‑101 (MQ=255)
                                                                    ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt                                  <  2:1766876/101‑1 (MQ=255)
                                                                     gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc                                 >  2:1123499/1‑101 (MQ=255)
                                                                     gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc                                 >  1:875311/1‑101 (MQ=255)
                                                                      tATTTGGAGCAAGATG.AAAGTCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                                <  1:380306/101‑1 (MQ=255)
                                                                      tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                                >  1:3176031/1‑101 (MQ=255)
                                                                       aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                               >  1:3761020/1‑101 (MQ=255)
                                                                       aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                               >  2:705936/1‑101 (MQ=255)
                                                                        tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa                              <  2:3686629/101‑1 (MQ=255)
                                                                        tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa                              <  1:1042070/101‑1 (MQ=255)
                                                                         ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                             <  2:2871811/101‑1 (MQ=255)
                                                                         ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                             >  2:809106/1‑101 (MQ=255)
                                                                           ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                           >  1:694005/1‑101 (MQ=255)
                                                                             agcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGt                         >  1:1831823/3‑101 (MQ=255)
                                                                              gcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGtt                        <  2:3548818/100‑1 (MQ=255)
                                                                               cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                       >  1:1841831/1‑101 (MQ=255)
                                                                               cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                       >  2:2001548/1‑101 (MQ=255)
                                                                                 aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg                     >  1:1029815/1‑101 (MQ=255)
                                                                                    atgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc                   >  1:4635115/4‑101 (MQ=255)
                                                                                     tgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCt                  >  2:413619/3‑101 (MQ=255)
                                                                                     tgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCt                  >  2:3048687/3‑101 (MQ=255)
                                                                                      gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc                 <  2:2075615/100‑1 (MQ=255)
                                                                                      gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc                 >  2:3882507/2‑101 (MQ=255)
                                                                                      gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc                 >  1:389622/2‑101 (MQ=255)
                                                                                      gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGAAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGGTCCGACTc                 >  2:2522902/2‑101 (MQ=255)
                                                                                        aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCCTTTCTCAAGCGTTCCGGCTCAg               >  1:1293108/1‑101 (MQ=255)
                                                                                          gACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGTGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAAaa             <  1:2976943/101‑1 (MQ=255)
                                                                                          gACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa             >  2:737620/1‑101 (MQ=255)
                                                                                          gACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa             <  1:2976942/101‑1 (MQ=255)
                                                                                           aCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc            <  2:3286691/101‑1 (MQ=255)
                                                                                           aCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc            <  1:1650446/101‑1 (MQ=255)
                                                                                           aCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc            >  2:1546798/1‑101 (MQ=255)
                                                                                           aCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGCCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGACTTCCTCAAGCGTTCCGGCTCAGAAc            >  1:2393147/1‑101 (MQ=255)
                                                                                              aTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCgg         >  1:4541274/1‑101 (MQ=255)
                                                                                                 ggTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAg      >  1:4236504/1‑101 (MQ=255)
                                                                                                  gTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGGAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGc     >  2:2275057/1‑101 (MQ=255)
                                                                                                  gTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGc     >  1:1011696/1‑101 (MQ=255)
                                                                                                   ttCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGcc    >  1:916523/1‑101 (MQ=255)
                                                                                                    tCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGccc   <  1:186052/101‑1 (MQ=255)
                                                                                                    tCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGccc   >  2:2553776/1‑101 (MQ=255)
                                                                                                    tCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGccc   <  1:75192/101‑1 (MQ=255)
                                                                                                     cATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcg  >  2:4076485/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
TGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCG  >  NC_014034/2640116‑2640316

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG