Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,715,979 A→G 100% F8L (TTC→CTC)  amaB ← N‑carbamoyl‑L‑amino acid amidohydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,715,9790A→G100.0% 999.8 263F8L (TTC→CTC) amaBN‑carbamoyl‑L‑amino acid amidohydrolase
Reads supporting (aligned to +/- strand):  new base (140/122):  ref base (0/0):  total (141/122)

Only 100 of 267 total aligned reads displayed.

ATCGGTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAAGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAAGAG  >  NC_014034/2715881‑2716076
                                                                                                  |                                                                                                 
atcggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGt                                                                                                 >  1:2560914/1‑101 (MQ=255)
atcggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGt                                                                                                 >  2:347072/1‑101 (MQ=255)
 tcggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGtt                                                                                                >  1:3231499/1‑101 (MQ=255)
   ggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGtttt                                                                                              >  2:4069521/1‑101 (MQ=255)
    gTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTtc                                                                                             >  2:1402998/1‑101 (MQ=255)
     tCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTtct                                                                                            >  1:1907837/1‑101 (MQ=255)
       aCGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTcc                                                                                          >  1:2370473/1‑101 (MQ=255)
         ggTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTtcagatc                                                                                        >  1:2521508/1‑96 (MQ=255)
          gTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGTCGCGCGGAATTGATACGGAGGTTTTCTCCggg                                                                                       >  1:1530088/1‑101 (MQ=255)
           tCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGt                                                                                      <  2:164470/101‑1 (MQ=255)
           tCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGGTTTCTCCGGGt                                                                                      >  1:4031848/1‑101 (MQ=255)
            cTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTg                                                                                     >  2:3888051/1‑101 (MQ=255)
             tGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTTTCCGGGTgt                                                                                    >  2:3781880/1‑100 (MQ=255)
             tGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGa                                                                                    <  2:3817680/101‑1 (MQ=255)
             tGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGa                                                                                    >  1:4507156/1‑101 (MQ=255)
             tGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGGTTTCTCCGGGCGa                                                                                    >  2:1262708/1‑101 (MQ=255)
              ggCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGAt                                                                                   <  2:2929400/101‑1 (MQ=255)
              ggCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGAt                                                                                   <  2:4220060/101‑1 (MQ=255)
              ggCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGGGAt                                                                                   >  1:4623466/1‑101 (MQ=255)
                cGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATgg                                                                                 <  2:2848731/101‑1 (MQ=255)
                 ggTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGc                                                                                <  1:3103859/101‑1 (MQ=255)
                 ggTGGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGc                                                                                <  2:416446/101‑1 (MQ=255)
                   ttgttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCAt                                                                              <  2:1125776/101‑1 (MQ=255)
                    tgttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATc                                                                             >  2:2664072/1‑101 (MQ=255)
                    tgttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATc                                                                             <  2:1854212/101‑1 (MQ=255)
                    tgttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGCTTTCTCCGGGTGATGGCATc                                                                             >  2:4126363/1‑101 (MQ=255)
                     gttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCt                                                                            >  2:485278/1‑101 (MQ=255)
                      gtgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTg                                                                           <  2:1170600/100‑1 (MQ=255)
                       tgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGAGACGGAGGTTTTCTCCGGGTGATGGCATCTgg                                                                          <  1:4216264/101‑1 (MQ=255)
                        gCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGc                                                                         <  2:1498152/101‑1 (MQ=255)
                        gCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGc                                                                         >  2:3837796/1‑101 (MQ=255)
                         ccccccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCt                                                                        <  2:1009702/101‑1 (MQ=255)
                          cccccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTg                                                                       <  1:2163720/101‑1 (MQ=255)
                               gcgATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTcc                                                                  >  2:4487594/1‑101 (MQ=255)
                                cgATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACATGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTccc                                                                 <  2:2416039/101‑1 (MQ=255)
                                cgATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTccc                                                                 >  1:4277550/1‑101 (MQ=255)
                                cgATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTccc                                                                 >  1:1033216/1‑101 (MQ=255)
                                cgATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTccc                                                                 >  1:358569/1‑101 (MQ=255)
                                cgATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTccc                                                                 >  1:1669687/1‑101 (MQ=255)
                                   tCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgt                                                              <  1:3481102/101‑1 (MQ=255)
                                   tCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgt                                                              <  1:1403092/101‑1 (MQ=255)
                                    ccccGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgtc                                                             <  1:949080/101‑1 (MQ=255)
                                        ggCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCt                                                         >  1:732988/1‑101 (MQ=255)
                                        ggCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCt                                                         <  1:2976915/101‑1 (MQ=255)
                                        ggCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGCGATGGCATCTGGCTGTCTCCCTGTCGTCt                                                         >  1:125631/1‑101 (MQ=255)
                                           ccGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCg                                                      <  1:2726635/101‑1 (MQ=255)
                                            cGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCgg                                                     <  2:62636/101‑1 (MQ=255)
                                             gACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCggg                                                    <  1:4353687/101‑1 (MQ=255)
                                             gACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCggg                                                    <  2:812660/101‑1 (MQ=255)
                                               ccTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGc                                                  <  1:4671312/101‑1 (MQ=255)
                                                cTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCt                                                 <  2:3051012/101‑1 (MQ=255)
                                                  tcgcCCTCTGCATGCGGCTGTCCCACAGGCGCGCGGAATTGATTCGGAGGTTTTCTCCTGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCttt                                               <  1:1432780/100‑1 (MQ=255)
                                                  gcgcCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCttt                                               <  1:1655154/101‑1 (MQ=255)
                                                  gcgcCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCttt                                               <  2:3680348/101‑1 (MQ=255)
                                                   tctcATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTg                                              <  1:3079606/98‑1 (MQ=255)
                                                   cgcCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTg                                              <  1:4555222/101‑1 (MQ=255)
                                                   cgcCATCTGCATGAGGCTGTCCCACAGGCGCGCAGATGTGATGGGGGGTTTCTCTCCGGGAGATG..ATATGGCGGTGTCCCTCTCGCGTGTgggggtctttc                                            >  2:141749/1‑94 (MQ=255)
                                                    gcCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGc                                             <  1:2655030/101‑1 (MQ=255)
                                                    gcCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGc                                             >  2:3489075/1‑101 (MQ=255)
                                                     ccATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGcc                                            >  1:50623/1‑101 (MQ=255)
                                                      cATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGccc                                           >  1:4113564/1‑101 (MQ=255)
                                                       aTCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCct                                          >  2:1304493/1‑101 (MQ=255)
                                                         cTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCctct                                        >  2:3461871/1‑101 (MQ=255)
                                                         cTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCctct                                        >  1:560727/1‑101 (MQ=255)
                                                           gCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTg                                      <  1:4487844/101‑1 (MQ=255)
                                                            gcagAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGc                                     >  2:4579473/4‑101 (MQ=255)
                                                            cATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGc                                     <  1:3762872/101‑1 (MQ=255)
                                                            cATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGc                                     >  1:3932815/1‑101 (MQ=255)
                                                               gAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGAc                                  <  1:377042/101‑1 (MQ=255)
                                                                aGGCTGTCCCACAGGCGCGCGGAATTGATACGGCGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACt                                 <  2:1929054/101‑1 (MQ=255)
                                                                 ggCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTc                                >  2:3136561/1‑101 (MQ=255)
                                                                   cTGTCCCACAGGCGCGCGGAATTGATACGAAGGTTTTCTCCGGGTGATGGCATGTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAt                              >  2:697957/1‑101 (MQ=255)
                                                                    tGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAtt                             >  1:128506/1‑101 (MQ=255)
                                                                    tGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAtt                             <  2:3271003/101‑1 (MQ=255)
                                                                    tGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAtt                             >  1:3170253/1‑101 (MQ=255)
                                                                     gTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAttt                            <  1:1937719/101‑1 (MQ=255)
                                                                       cccACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTc                          >  2:275090/1‑101 (MQ=255)
                                                                       cccACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTc                          >  1:544092/1‑101 (MQ=255)
                                                                         cacaGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTg                        <  1:1856083/101‑1 (MQ=255)
                                                                           caGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAc                      <  1:3888293/101‑1 (MQ=255)
                                                                           caGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAc                      >  1:874817/1‑101 (MQ=255)
                                                                            aGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAcc                     >  1:2254361/1‑101 (MQ=255)
                                                                            aGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAcc                     >  2:2556351/1‑101 (MQ=255)
                                                                            aGGCGCGCCGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAcc                     <  2:4406909/101‑1 (MQ=255)
                                                                             ggCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCg                    >  1:3314939/1‑101 (MQ=255)
                                                                                 cgcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTg                >  2:3101612/1‑101 (MQ=255)
                                                                                     gAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCa            <  2:90040/101‑1 (MQ=255)
                                                                                     gAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCa            >  2:1688791/1‑101 (MQ=255)
                                                                                      aaTTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCag           >  2:1938891/1‑101 (MQ=255)
                                                                                       aTTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCaga          >  2:2239212/1‑101 (MQ=255)
                                                                                        ttGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCagag         >  2:2465779/1‑101 (MQ=255)
                                                                                         tGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAgg        >  2:2168885/1‑101 (MQ=255)
                                                                                         tGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAgg        >  1:3548162/1‑101 (MQ=255)
                                                                                          gATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGa       >  2:4337606/1‑101 (MQ=255)
                                                                                           aTACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGaa      <  1:2664226/101‑1 (MQ=255)
                                                                                             aCGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAag    <  1:36376/101‑1 (MQ=255)
                                                                                              cGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAaga   >  1:2340039/1‑101 (MQ=255)
                                                                                              cGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCCCCCTGTCGTCTGCGGGGCTTTGCCCTTTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAaga   <  2:2560762/101‑1 (MQ=255)
                                                                                               ggAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAagag  <  2:4651112/101‑1 (MQ=255)
                                                                                               ggAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAagag  >  2:1069237/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
ATCGGTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAAGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAAGAG  >  NC_014034/2715881‑2716076

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG