BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,068,163	A→T	3.0%	intergenic (‑146/+146)	glcD ← / ← RCAP_rcc02872	glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,068,163	0	A→T	3.0%	6.2 ‑1.1 ‑0.3	167	intergenic (‑146/+146)	glcD/RCAP_rcc02872	glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand): new base (5/0): ref base (109/52): total (114/53)
Fisher's exact test strand distribution p-value = 3.26e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.62e-01

Only 100 of 232 total aligned reads displayed.


ATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGC  >  NC_014034/3068063‑3068263


aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCa                                                                                                         >  2:1115701/1‑101 (MQ=255)


aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCa                                                                                                         <  2:200985/101‑1 (MQ=255)


aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCa                                                                                                         >  2:956144/1‑101 (MQ=255)


 ttCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAg                                                                                                        <  1:4642380/101‑1 (MQ=255)


          ccGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCgcccgccc                                                                                               <  2:28085/101‑1 (MQ=255)


           cGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGTGCCCAAA.GGCCCTC.GG.CCAGCGcccgcccc                                                                                              <  1:4227126/101‑1 (MQ=255)


            gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGcccgccccg                                                                                             >  2:2260783/1‑101 (MQ=255)


            gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGcccgccccc                                                                                             >  2:2420912/1‑100 (MQ=255)


            gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGcccgccccc                                                                                             >  2:43076/1‑100 (MQ=255)


             aaaCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGGCCTC.GG.CCCGCGcccgccccgc                                                                                            >  2:499072/1‑101 (MQ=255)


               aCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCcgccccgccc                                                                                          <  1:3006811/101‑1 (MQ=255)


                cGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCa                                                                                         >  2:1263252/1‑101 (MQ=255)


                cGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCa                                                                                         <  1:1108846/101‑1 (MQ=255)


                cGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTA.GG.CCAGCGCCCGCCCCGCCCa                                                                                         <  1:4165361/101‑1 (MQ=255)


                 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCAt                                                                                        <  1:1401396/101‑1 (MQ=255)


                  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCacg                                                                                       >  2:4442714/1‑99 (MQ=255)


                  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCacg                                                                                       >  1:3896909/1‑99 (MQ=255)


                  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATg                                                                                       >  2:743085/1‑101 (MQ=255)


                  aTGGGCAGGCGGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATg                                                                                       <  1:1209557/101‑1 (MQ=255)


                   tGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GA.CCAGCCGCCGCCCCGCCCATgg                                                                                      >  2:803277/1‑101 (MQ=255)


                           cTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.t                                                                           >  2:1381513/1‑101 (MQ=255)


                            tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.tt                                                                          <  1:4685412/101‑1 (MQ=255)


                            tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.tt                                                                          >  2:183647/1‑101 (MQ=255)


                              gccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.Ttct                                                                        <  1:1989997/100‑1 (MQ=255)


                              aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.Ttct                                                                        >  1:2361329/1‑101 (MQ=255)


                               ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.Ttctc                                                                       <  1:2428552/101‑1 (MQ=255)


                                cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTcg                                                                      <  2:30935/101‑1 (MQ=255)


                                cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTcg                                                                      >  1:3503987/1‑101 (MQ=255)


                                cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCATCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTcg                                                                      <  1:4203364/101‑1 (MQ=255)


                                 gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATggcgggc.ggcggcccc                                                                       >  2:3509675/1‑93 (MQ=255)


                                 gAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGG.G.C.TTCTcgc                                                                     >  2:2553393/1‑101 (MQ=255)


                                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTcgcc                                                                    <  1:2924713/101‑1 (MQ=255)


                                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTcgcc                                                                    >  2:2202048/1‑101 (MQ=255)


                                   ggCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAC.GGCCATC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTcgccc                                                                   <  2:2079043/101‑1 (MQ=255)


                                   ggCCAGCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTcgccc                                                                   <  2:3264755/101‑1 (MQ=255)


                                     ccATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGG.G.C.TTCTcgccccc                                                                 >  1:805225/1‑99 (MQ=255)


                                     ccATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTcgccccc                                                                 >  2:1686245/1‑99 (MQ=255)


                                      cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCCCCCATGGCGGGC.G.C.TTCTcccccccc                                                                >  1:2663382/1‑101 (MQ=255)


                                       aTCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCccgccg                                                               <  1:3686174/101‑1 (MQ=255)


                                        tCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCgg                                                              <  2:4188386/101‑1 (MQ=255)


                                          gCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCgggg                                                            <  1:2815010/101‑1 (MQ=255)


                                          gCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCgggg                                                            <  1:1285927/101‑1 (MQ=255)


                                           ccGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGgggc                                                           >  2:266490/1‑101 (MQ=255)


                                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGgggcg                                                          <  1:3844814/101‑1 (MQ=255)


                                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGgggcg                                                          <  1:988171/101‑1 (MQ=255)


                                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGgggcg                                                          >  2:2715033/1‑101 (MQ=255)


                                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.CTCTCGCCCCCCCgggcg                                                          >  2:1892998/1‑101 (MQ=255)


                                             gAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCCgggcgg                                                         >  2:648483/1‑101 (MQ=255)


                                             gAGGGCATGCGGACCAGTGGCCTCGGGCCCGCCGAGGCCCCAAA.GGCCCTC.GG.CCAGCCCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGgggcgg                                                         <  1:2185858/101‑1 (MQ=17)


                                              aGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGgggcggg                                                        >  1:2000735/1‑101 (MQ=255)


                                               gctgagtgggAACAGTGTCCTCGGGCCCGCCGAGAGCCCAAA.GGCCCTC.GG.CCAGGGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGgggcgggc                                                       <  1:2570691/93‑1 (MQ=14)


                                                gTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGgggcggggg                                                      >  1:4462986/1‑99 (MQ=255)


                                                 tCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGG.G.C.TTCTCGCCCGCCGgggcggggcc                                                     >  1:2738004/1‑98 (MQ=255)


                                                  cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGGGcc                                                    >  1:3151551/1‑101 (MQ=255)


                                                   aggCGGAACAGTGGCCGCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCGGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCg                                                   <  1:647431/99‑1 (MQ=255)


                                                    tGCGGAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCgg                                                  <  1:1019091/101‑1 (MQ=255)


                                                      gggAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGcc                                                <  2:4213703/100‑1 (MQ=255)


                                                        gAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGGGCCGGcccc                                              >  1:1370634/1‑99 (MQ=255)


                                                        gAACAGTGGCCGCGTGCCCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCctc                                              <  2:4398943/101‑1 (MQ=255)


                                                         aaCAGTGGTCCCATGACCCCCGAGTGCCCCAA.GGCACTC.GG.GCAGCGCCCGCCCCGCCCCTTGCGGGCGG.C.TCCCGGCCCGCGGGGGACGGCCCCGGcccc                                              >  1:9399/1‑99 (MQ=12)


                                                          aCAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCccctc                                            >  2:3639427/1‑100 (MQ=255)


                                                           cgggggCCTCGTGCCCCCCGAGGCCCAAAA.GGCCCTC.GG.CCACCGCCCGCCCCGCCCATGGGGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTctgc                                           <  1:1160519/97‑1 (MQ=14)


                                                           caatGGCCCCGTGACCGGCGAGGGCCCAAA.GGCCCTC.GG.CCTGCGCCCGCCCCGCCATCGGCGGGC.G.CGGTCGCGCCGCCCGGGGGGGGGGGCgggccccg                                            >  2:1955833/4‑95 (MQ=17)


                                                             gTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCCGGGCGGGGGCCCGCCCctgctg                                         >  2:2117713/1‑101 (MQ=17)


                                                              tGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCCGcccccgccgc                                        >  1:4530412/1‑93 (MQ=255)


                                                              tGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCCGCCCctgctcc                                        >  2:2444994/1‑99 (MQ=255)


                                                              tGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCCCCCGGGAGGGGGCCAGCCTCtgctgc                                        >  1:3644631/1‑101 (MQ=17)


                                                              ggcccTCGGGCCCGCCGAGGGCCCAAA.GGCCCTC.GG.CCGGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCtgctgc                                        <  2:3617322/98‑1 (MQ=14)


                                                               ggCCCCGTGACCGGCGAGGGCCCAAA.GGCCCTC.GG.CCTGCGCCCGCCCCGCCATCGGCGGGCGG.C.GTCGCGCCCCCCGGGGCGGGGGCGgggccgccggtg                                        >  1:919207/1‑91 (MQ=14)


                                                                    cGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGGGCCGGCCTCTGCTGCGCCTgg                                  >  2:1694837/1‑101 (MQ=255)


                                                                     gggcccgccgAGGCCCCAAG.GCCCCTC.GG.CCAGCCCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTggg                                 <  1:333918/97‑1 (MQ=14)


                                                                       gACCGCCGAGGGCCCAAA.GGCACTC.GG.CCTGCGCCCGCCCCGCCATCGGCGGGG.GCC.CGCGCGCCCCCCGGGGGGGGGGGCGGCCCccgcttctgtttggg                                >  2:106655/1‑91 (MQ=2)


                                                                         ccgccgGGGCCCCAAA.GCCCCTC.GC.CCAGCCCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGaaa                             <  1:183672/101‑1 (MQ=14)


                                                                           gacgcggccccAAT.GGCCCTC.GA.CCAGCCCCCCCCCCGCACGTGGCGGGC.G.C.TTCTGGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACg                           <  2:3896667/93‑1 (MQ=17)


                                                                            ccgGGGGCCAAAG.GGCCCCC.GC.CCGGCCCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACgg                          <  1:3529521/101‑1 (MQ=17)


                                                                            ccgAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACgg                          >  2:1625367/1‑101 (MQ=255)


                                                                            cccggggCCCAAACGCCCCGC.GG.CCGGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACg                           <  2:1959938/97‑1 (MQ=14)


                                                                             cgggggCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGt                         <  1:1719466/98‑1 (MQ=255)


                                                                             ccggggCCCAAA.GGCCCCC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGG.G.C.TTCTCCCCCCCCGGGGCGGGGGCCGGCCCCTGTCACGCCTtggggcaccgg                         >  1:1033255/4‑94 (MQ=14)


                                                                              gggggCCCAAA.GGCCGCC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGG.G.G.GTCTCGCCCCCCCGGCCCGGCGGCGGGCCCGGGGGCGCCTcgggggcggggc                        >  2:2485955/3‑93 (MQ=2)


                                                                              gAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCCCCGCCGCGCCCCGGGCAAccggt                        >  2:1305220/1‑97 (MQ=11)


                                                                                 ggCCC.AA.GGCCCCC.GGCCCGGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTg                     <  1:4623573/101‑1 (MQ=17)


                                                                                  gccgcaa.GCCCCGC.GG.CCGGCCCCCGCCCCGCCCATGGCGGTC.G.C.TTCTCGCCCGCCGGGGCGGGCGCTGGCCTCTGTTGCACTTGGGGAAACGGTcgcgg                    <  1:1522563/96‑5 (MQ=14)


                                                                                  gcccgaagggCCCCC.GC.CCGCCGCCCCCCCCGCCCATGGCGGGC.G.T.TTCGCGCCGGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTg                     <  2:2913722/93‑1 (MQ=14)


                                                                                  gCCCAAAGGGCCCTC.GC.CCGGCGCCCGCCCCGCCCATGGGGGGC.G.C.TTCTCGCCCGCCGGGGGGGGCCCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTg                     <  2:45566/101‑1 (MQ=17)


                                                                                   cccCAA.GGCCCCCGGG.CCGGCGCCCCCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTgg                    <  2:3210680/101‑1 (MQ=17)


                                                                                   cccAAG.GGCCCCC.GG.CCAGCGCCCCCCCCGCCCGTGGCGGGC.G.C.TTCTCGCCCGCGGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGc                   <  1:1889427/101‑1 (MQ=17)


                                                                                   cccAAG.GGCCCCC.GG.CCAGCCCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGc                   <  2:1808563/101‑1 (MQ=255)


                                                                                     cAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCCGGGCGGGGGCCCGCCTCCGCTGCGCCTGGGGGAACAGGCCTGGCAc                 >  1:1959265/1‑101 (MQ=14)


                                                                                       aa.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCCGGGGGAACGGTCTTTGGAccc               >  1:2267131/1‑100 (MQ=17)


                                                                                       aa.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCCGCCTCTTCTGCGCCCGGGGAAACGGTCTTgggcccg               >  2:3954947/1‑96 (MQ=17)


                                                                                          ggCCCGC.GC.CCACCGCCCCCCCCCCCNNGGNCGGGC.C.C.TTCGCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGcc             <  1:3244349/101‑1 (MQ=18)


                                                                                           gCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCGGCTGCGCCTGGGGAAACGGGCTTGGCACCGccc            >  1:4053501/1‑101 (MQ=255)


                                                                                           ccccTG.GC.CCAGCCCCCGCCCCGCCCAGGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGccc            <  2:953175/100‑1 (MQ=18)


                                                                                            cccTC.GC.CCGGCCCCCCCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCg           <  1:4413423/101‑1 (MQ=18)


                                                                                               cc.GG.CCCGGGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGc        <  1:3618130/100‑1 (MQ=255)


                                                                                                c.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCt       >  1:754377/1‑101 (MQ=255)


                                                                                                c.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCCCCCGCCGGGGCGGGCGCCGGCCTCCGCTCCGCCTGGGGGAACGGGCTTGGGCCCGCCCGGGcc       >  1:1816988/1‑100 (MQ=14)


                                                                                                  gg.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGGCGGCCTCTGCCGCGGCTGGGGGAAGGGGCTTAGCACCGCCCCGGCTc      >  1:2794653/1‑101 (MQ=17)


                                                                                                       aGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCcggc  >  2:3448738/1‑101 (MQ=255)


ATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GGCCCTC.GG.CCAGCGCCCGCCCCGCCCATGGCGGGC.G.C.TTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGC  >  NC_014034/3068063‑3068263

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 22 ≤


ATCG

< 32 ≤


ATCG

< 40 ≤


ATCG