BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	539,743	T→G	100%	Y201D (TAC→GAC)	RCAP_rcc00505 →	type II secretion system protein E

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	539,743	0	T→G	100.0%	905.4	247	Y201D (TAC→GAC)	RCAP_rcc00505	type II secretion system protein E
Reads supporting (aligned to +/- strand): new base (141/105): ref base (0/0): total (142/105)

Only 100 of 255 total aligned reads displayed.


CGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCTACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACAA  >  NC_014034/539645‑539840


cGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGAc                                                                                                 <  1:1087869/101‑1 (MQ=255)


cGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGAc                                                                                                 <  1:1304420/101‑1 (MQ=255)


 ggACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACg                                                                                                >  1:1684742/1‑101 (MQ=255)


  gACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGc                                                                                               >  2:1435762/1‑101 (MQ=255)


   aCGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGcc                                                                                              <  2:2637976/101‑1 (MQ=255)


   aCGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGcc                                                                                              >  1:594224/1‑101 (MQ=255)


   aCGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGcc                                                                                              >  1:1131753/1‑101 (MQ=255)


    cGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGccc                                                                                             >  2:625153/1‑101 (MQ=255)


    cGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGGGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGccc                                                                                             >  1:1974397/1‑101 (MQ=255)


      tGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGt                                                                                           >  2:450166/1‑101 (MQ=255)


      tGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGt                                                                                           >  2:4031924/1‑101 (MQ=255)


      tGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGt                                                                                           >  1:2177973/1‑101 (MQ=255)


      tGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGt                                                                                           >  2:828339/1‑101 (MQ=255)


        aCCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCt                                                                                         >  1:3168184/1‑101 (MQ=255)


           ttCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGc                                                                                      >  2:4418030/1‑101 (MQ=255)


            tCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGcc                                                                                     >  2:1869913/1‑101 (MQ=255)


             cAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCg                                                                                    <  1:792745/101‑1 (MQ=255)


             cAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCg                                                                                    >  1:1865254/1‑101 (MQ=255)


             cAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCg                                                                                    >  2:4572128/1‑101 (MQ=255)


               aGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGAt                                                                                  >  1:1417006/1‑101 (MQ=255)


                ggATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATg                                                                                 <  2:1289945/101‑1 (MQ=255)


                 gATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATgg                                                                                >  2:2207260/1‑101 (MQ=255)


                  aTGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGc                                                                               <  2:2293864/101‑1 (MQ=255)


                  aTGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGc                                                                               <  1:990195/101‑1 (MQ=255)


                  aTGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGc                                                                               >  2:3652171/1‑101 (MQ=255)


                   tGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCt                                                                              <  1:4626798/101‑1 (MQ=255)


                    gAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTc                                                                             <  2:2860381/101‑1 (MQ=255)


                     aaaaGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTcg                                                                            >  2:1233047/1‑101 (MQ=255)


                     aaaaGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTcg                                                                            >  1:2336438/1‑101 (MQ=255)


                        aGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGt                                                                         <  2:2751171/101‑1 (MQ=255)


                          cACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGttt                                                                       <  1:3957133/101‑1 (MQ=255)


                          cACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGttt                                                                       <  2:377430/101‑1 (MQ=255)


                          cACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGttt                                                                       <  1:1568685/101‑1 (MQ=255)


                           aCCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTc                                                                      >  2:1418391/1‑101 (MQ=255)


                            ccTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCa                                                                     <  1:370562/101‑1 (MQ=255)


                            ccTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCa                                                                     >  2:2482268/1‑101 (MQ=255)


                            ccTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCa                                                                     <  1:24213/101‑1 (MQ=255)


                            ccTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCa                                                                     <  1:625217/101‑1 (MQ=255)


                              tGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAc                                                                   >  2:1547269/1‑101 (MQ=255)


                               gTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAcg                                                                  >  2:4337714/1‑101 (MQ=255)


                               gTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAcg                                                                  >  2:1643149/1‑101 (MQ=255)


                               gTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAcg                                                                  >  1:856670/1‑101 (MQ=255)


                               gTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAcg                                                                  >  2:3869577/1‑101 (MQ=255)


                                ttGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAcgc                                                                 >  1:3575847/1‑101 (MQ=255)


                                 tGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAcgcg                                                                >  2:4156175/1‑101 (MQ=255)


                                 tGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAcgcg                                                                <  2:3992147/101‑1 (MQ=255)


                                       tcatcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTg                                                          >  2:1361633/1‑101 (MQ=255)


                                        catcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGc                                                         >  1:1631061/1‑101 (MQ=255)


                                        catcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGc                                                         <  2:2328752/101‑1 (MQ=255)


                                         atcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGcc                                                        >  1:4034619/1‑101 (MQ=255)


                                          tcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGccc                                                       <  1:2792005/101‑1 (MQ=255)


                                             aCAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCg                                                    <  2:3044099/101‑1 (MQ=255)


                                             aCAAGATCATGTCCTCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCg                                                    <  1:4100365/101‑1 (MQ=255)


                                              cAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGACCCCgg                                                   >  1:766741/1‑101 (MQ=255)


                                               aaGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAGCCCCTATGTCGATGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCGCCGGt                                                  <  2:213631/101‑1 (MQ=255)


                                               aaGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCGggc                                                  >  2:2922474/1‑100 (MQ=255)


                                                aGATCGGGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTc                                                 <  2:4375565/101‑1 (MQ=255)


                                                      tgtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTg                                           <  1:4191294/101‑1 (MQ=255)


                                                        tCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGa                                         <  2:3702140/101‑1 (MQ=255)


                                                         ccGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGAt                                        >  1:3375044/1‑101 (MQ=255)


                                                         ccGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGAt                                        <  2:3293339/101‑1 (MQ=255)


                                                         ccGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGGGGAt                                        >  2:2047850/1‑101 (MQ=255)


                                                           gcgGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATgg                                      >  2:1953867/1‑101 (MQ=255)


                                                           gcgGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATgg                                      >  1:4307753/1‑101 (MQ=255)


                                                           gcgGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAAGCGATGGTGCCCCCGGTCGCGGTGGATgg                                      >  2:2672775/1‑101 (MQ=255)


                                                             ggTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgct                                    >  2:716228/1‑101 (MQ=255)


                                                             ggTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgct                                    >  2:1788128/1‑101 (MQ=255)


                                                             ggTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgct                                    >  2:3946039/1‑101 (MQ=255)


                                                             ggTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgct                                    <  1:4597294/101‑1 (MQ=255)


                                                             ggTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGAGGgct                                    >  2:4216764/1‑101 (MQ=255)


                                                               tGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctcg                                  >  1:4361932/1‑101 (MQ=255)


                                                                 gggCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctcgct                                <  1:3652400/101‑1 (MQ=255)


                                                                 gggCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctcgct                                >  1:2442323/1‑101 (MQ=255)


                                                                  ggcggcGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctcgctc                               <  1:2839757/101‑1 (MQ=255)


                                                                  ggcggcGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctcgctc                               >  2:1213134/1‑101 (MQ=255)


                                                                    cggcgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGt                             <  1:2896649/101‑1 (MQ=255)


                                                                    cggcgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGt                             <  1:4465229/101‑1 (MQ=255)


                                                                      gcgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGtct                           >  2:3980438/1‑101 (MQ=255)


                                                                       cgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGtctc                          <  1:3028284/101‑1 (MQ=255)


                                                                        gtttCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCg                         <  1:2672929/98‑1 (MQ=255)


                                                                         tgtCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGa                        >  2:1097678/1‑101 (MQ=255)


                                                                         tgtCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCCCGa                        >  2:3007681/1‑101 (MQ=255)


                                                                         ggtCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGa                        <  2:746450/100‑1 (MQ=255)


                                                                          gtCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGAt                       >  1:3905134/1‑101 (MQ=255)


                                                                            cGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATcc                     <  1:4418280/101‑1 (MQ=255)


                                                                            cGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGAGGGCTCGCTCGGCCCGATcc                     >  2:3810964/1‑101 (MQ=255)


                                                                            cGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGGGGATGGCTCGCTCGTCTCGATcc                     >  2:1608160/1‑101 (MQ=255)


                                                                             gATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCg                    >  1:3684401/1‑101 (MQ=255)


                                                                             gATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCg                    >  2:4081465/1‑101 (MQ=255)


                                                                               tGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGa                  <  2:856596/101‑1 (MQ=255)


                                                                                gAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGaa                 <  2:4300361/101‑1 (MQ=255)


                                                                                  aaGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAAt               <  1:742218/101‑1 (MQ=255)


                                                                                   aGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAAtt              <  1:1380335/101‑1 (MQ=255)


                                                                                     cAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCa            <  1:1435856/101‑1 (MQ=255)


                                                                                        ccccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaaga         >  2:3445614/1‑101 (MQ=255)


                                                                                          ccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaagaag       >  1:2261747/1‑101 (MQ=255)


                                                                                          ccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaagaag       <  2:3268698/101‑1 (MQ=255)


                                                                                          ccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaagaag       <  1:1281895/101‑1 (MQ=255)


                                                                                               gTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACaa  <  2:460780/101‑1 (MQ=255)


                                                                                               gTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACaa  <  2:2385241/101‑1 (MQ=255)


CGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCTACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACAA  >  NC_014034/539645‑539840

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG