Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,307,862 3 bp→GGC 100% coding (933‑935/1203 nt) RCAP_rcc02140 ← extracellular ligand‑binding receptor

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,307,8620C→G100.0% 33.6 161G312A (GGC→GCC) RCAP_rcc02140extracellular ligand‑binding receptor
Reads supporting (aligned to +/- strand):  new base (60/86):  ref base (14/1):  total (74/87)
Fisher's exact test strand distribution p-value = 1.37e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00
*NC_0140342,307,8630C→G100.0% 589.5 161G312R (GGC→CGC) RCAP_rcc02140extracellular ligand‑binding receptor
Reads supporting (aligned to +/- strand):  new base (74/85):  ref base (0/1):  total (74/87)
*NC_0140342,307,8640G→C100.0% 33.9 160N311K (AAC→AAGRCAP_rcc02140extracellular ligand‑binding receptor
Reads supporting (aligned to +/- strand):  new base (59/84):  ref base (14/1):  total (73/87)
Fisher's exact test strand distribution p-value = 1.38e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

TAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CGCCG.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCG  >  NC_014034/2307772‑2307952
                                                                                            |||                                                                                         
 aGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCgccgcc                                                                                 >  1:250462/1‑101 (MQ=255)
 aGCGGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCgccgcc                                                                                 <  2:2034461/101‑1 (MQ=255)
  gCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGccgccg                                                                                >  2:879202/1‑101 (MQ=255)
  gCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGccgccg                                                                                >  1:4452632/1‑101 (MQ=255)
   cTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCgg                                                                               >  1:4333080/1‑101 (MQ=255)
     ggAACGGTCCGGGTTCGCCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATATCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGAt                                                                             <  1:4199865/101‑1 (MQ=255)
      gAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATc                                                                            >  1:1200207/1‑101 (MQ=255)
       aaCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CACGGC..TTCGCCGCCGGATcc                                                                           <  1:342901/101‑1 (MQ=255)
       aaCGGGGCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATcc                                                                           <  1:4661895/101‑1 (MQ=255)
        gcgggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCa                                                                          <  2:3194021/100‑1 (MQ=255)
        aCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCa                                                                          >  2:3067109/1‑101 (MQ=255)
         cgggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAg                                                                         >  2:3696753/1‑101 (MQ=255)
         cgggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAg                                                                         >  1:3915165/1‑101 (MQ=255)
          gggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAgc                                                                        >  1:634055/1‑101 (MQ=255)
          gggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAgc                                                                        >  1:2983110/1‑101 (MQ=255)
          gggtcgggtTCGCCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAgc                                                                        <  2:1593965/101‑1 (MQ=255)
          gggtcgggtTCGCCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAgc                                                                        <  1:92849/101‑1 (MQ=255)
             tcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGcgcc                                                                     <  1:214333/101‑1 (MQ=255)
             ccgggtCCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGcgcc                                                                     <  2:470583/100‑1 (MQ=255)
              cgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGcgccg                                                                    <  2:4332833/101‑1 (MQ=255)
              cgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGcgccg                                                                    >  2:4424059/1‑101 (MQ=255)
              cgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGcgccg                                                                    >  1:2730736/1‑101 (MQ=255)
              cgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGcgccg                                                                    >  2:677596/1‑101 (MQ=255)
               gggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGcgccgc                                                                   >  2:723168/1‑101 (MQ=255)
               gggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGcgccgc                                                                   >  2:1485080/1‑101 (MQ=255)
               gggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGcgccgc                                                                   >  1:3645375/1‑101 (MQ=255)
                ggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCcgcg                                                                  >  2:116089/1‑101 (MQ=255)
                   tCGGCCCCGCCGCGGCCAGCGCCGCCTGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgc                                                               <  2:789945/101‑1 (MQ=255)
                   tCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgc                                                               <  1:3650750/101‑1 (MQ=255)
                    cGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcg                                                              >  2:1071127/1‑101 (MQ=255)
                    cGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcg                                                              >  1:4419583/1‑101 (MQ=255)
                     ggCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcgc                                                             >  1:425099/1‑101 (MQ=255)
                        cccGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGcgccgc                                                          >  2:1131810/1‑101 (MQ=255)
                         ccgccgCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCgccgcc                                                         <  1:1900554/101‑1 (MQ=255)
                          cgccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCt                                                        <  2:1842486/101‑1 (MQ=255)
                             cgcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGAt                                                     >  2:3384986/1‑101 (MQ=255)
                             cgcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGAt                                                     <  2:536095/101‑1 (MQ=255)
                             cgcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TCCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGAt                                                     <  1:4420391/101‑1 (MQ=255)
                              gcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATa                                                    <  1:677660/101‑1 (MQ=255)
                              gcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATa                                                    <  2:1621877/101‑1 (MQ=255)
                               cgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAg                                                   <  2:1532760/101‑1 (MQ=255)
                                ggCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgc                                                  >  1:1259881/1‑101 (MQ=255)
                                 gccagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcg                                                 >  1:4497111/1‑101 (MQ=255)
                                 gccagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgag                                                 >  2:1253838/1‑99 (MQ=255)
                                  ccagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcgc                                                <  2:2904744/101‑1 (MQ=255)
                                   cagcgccgccagagccAGACGGCCGGTCAGATAGCCCTCGAAGGAGCCGAAGAC.CGGC..TTCGCCGCCGGCACCCGCGCCGCCGGAGCCGCCTGACAgggag                                               >  2:3353768/1‑97 (MQ=255)
                                     gcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGcc                                             <  1:2938843/101‑1 (MQ=255)
                                       gccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGccac                                           >  1:82623/1‑101 (MQ=255)
                                        ccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGccacc                                          <  1:2504937/101‑1 (MQ=255)
                                        ccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGccacc                                          <  1:2415671/101‑1 (MQ=255)
                                          gccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCcaccac                                        >  2:1620937/1‑101 (MQ=255)
                                          gccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCcaccac                                        <  1:2056041/101‑1 (MQ=255)
                                          gccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCcaccac                                        <  1:3294339/101‑1 (MQ=255)
                                           ccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCaccacc                                       <  1:3840659/101‑1 (MQ=255)
                                           ccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCaccacc                                       >  2:4526329/1‑101 (MQ=255)
                                             agcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCgg                                     <  2:4519399/101‑1 (MQ=255)
                                             agcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCgg                                     <  1:3772676/101‑1 (MQ=255)
                                             agcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCgg                                     <  2:549029/101‑1 (MQ=255)
                                              gcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGa                                    <  2:2391951/101‑1 (MQ=255)
                                               cgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGaa                                   <  1:227743/101‑1 (MQ=255)
                                                gccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAAt                                  <  1:1809009/101‑1 (MQ=255)
                                                gccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAAt                                  <  1:2614297/101‑1 (MQ=255)
                                                 ccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATg                                 <  2:3346263/101‑1 (MQ=255)
                                                 ccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATg                                 <  1:1072744/101‑1 (MQ=255)
                                                 ccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATg                                 <  1:1661395/101‑1 (MQ=255)
                                                  cagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGt                                >  2:143964/1‑101 (MQ=255)
                                                   agACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTc                               <  2:4133525/101‑1 (MQ=255)
                                                   agACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTc                               >  2:3598974/1‑101 (MQ=255)
                                                   agACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTc                               >  2:208605/1‑101 (MQ=255)
                                                     aCGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCgg                             <  2:2168690/101‑1 (MQ=255)
                                                       ggCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGgtg                           >  2:1643052/1‑101 (MQ=255)
                                                         ccGGCCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTc                         <  1:2205126/101‑1 (MQ=255)
                                                          cGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCg                        <  1:1340357/101‑1 (MQ=255)
                                                          cGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCg                        <  1:2825409/101‑1 (MQ=255)
                                                            gTCAGATAGCCCTCGAAGGAGACGAAG.C.C.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGaa                      >  1:4552160/1‑101 (MQ=255)
                                                             tCAGATAGCCCTCGAAGGAGACGAAG.C.CCGTC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAAc                     <  1:1394917/101‑1 (MQ=255)
                                                             tCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGCTAGCGCGCCACCACCGGAATGTCGGAGTCGAAc                     >  2:23481/1‑101 (MQ=255)
                                                             tCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAAc                     <  1:85945/101‑1 (MQ=255)
                                                             tCAGATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAAc                     <  1:2784059/101‑1 (MQ=255)
                                                              cAGATAGCCCTCGAAGGAGACGAAG.C.C.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACg                    >  2:2760219/1‑101 (MQ=255)
                                                              cAGATAGCCCTCGAAGGAGACGAAG.C.C.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACg                    >  1:4184335/1‑101 (MQ=255)
                                                               aGATAGCCCTCGAAGGAGACGAAG.C.C.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACgg                   >  2:1990145/1‑101 (MQ=255)
                                                                gATAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGa                  >  1:1733174/1‑101 (MQ=255)
                                                                 aTAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGAt                 >  2:2830247/1‑101 (MQ=255)
                                                                 aTAGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGAt                 <  2:1308290/101‑1 (MQ=255)
                                                                   aGCCCTCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAg               <  2:4184089/101‑1 (MQ=255)
                                                                       cTCGAAGGAGACGAAG.C.CCGGT.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCCCCGGAATGTCGGTGTCGAACGGATAGGGCa           <  1:1282590/101‑1 (MQ=255)
                                                                        tCGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCAc          <  2:2982922/101‑1 (MQ=255)
                                                                        tCGAAGGAGACGAAG.C.C.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCAc          >  2:2077981/1‑101 (MQ=255)
                                                                        ccGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCAc          <  2:418857/100‑1 (MQ=255)
                                                                         cGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACg         >  1:2959661/1‑101 (MQ=255)
                                                                         cGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACg         <  1:2021178/101‑1 (MQ=255)
                                                                         cGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACg         >  1:1563837/1‑101 (MQ=255)
                                                                         cGAAGGAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACg         <  1:527873/101‑1 (MQ=255)
                                                                           aaGGAGACGAAG.C.C.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAc       >  2:4288510/1‑101 (MQ=255)
                                                                            aGGAGACGAAG.C.C.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAcc      >  1:324830/1‑101 (MQ=255)
                                                                             ggAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCt     <  2:4452382/101‑1 (MQ=255)
                                                                             ggAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCt     >  2:1049369/1‑101 (MQ=255)
                                                                             ggAGACGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCt     <  2:122008/101‑1 (MQ=255)
                                                                                gaCGAAG.C.CCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCg  >  2:2856101/1‑101 (MQ=255)
                                                                                            |||                                                                                         
TAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.C.CGCCG.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCG  >  NC_014034/2307772‑2307952

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG