BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,620,690	2 bp→CT	100%	coding (1050‑1051/1188 nt)	livJ →	branched‑chain amino acid ABC transporter periplasmic branched‑chain amino acid‑binding protein LivJ

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,620,690	0	T→C	100.0%	878.5	228	P350P (CCT→CCC)	livJ	branched‑chain amino acid ABC transporter periplasmic branched‑chain amino acid‑binding protein LivJ
Reads supporting (aligned to +/- strand): new base (118/110): ref base (0/0): total (118/110)
*	NC_014034	2,620,691	0	A→T	100.0%	902.2	228	N351Y (AAC→TAC)	livJ	branched‑chain amino acid ABC transporter periplasmic branched‑chain amino acid‑binding protein LivJ
Reads supporting (aligned to +/- strand): new base (117/110): ref base (1/0): total (118/110)

Only 100 of 231 total aligned reads displayed.


GAATATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCTAACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACAA  >  NC_014034/2620595‑2620785

||


 aaTATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATg                                                                                           <  1:3538825/101‑1 (MQ=255)


  atatatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGa                                                                                          <  1:886709/101‑1 (MQ=255)


    atatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAg                                                                                        >  2:4057095/1‑101 (MQ=255)


      atCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAggg                                                                                      >  1:410100/1‑101 (MQ=255)


      atCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAggg                                                                                      <  1:554626/101‑1 (MQ=255)


      atCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAggg                                                                                      >  1:3265723/1‑101 (MQ=255)


          tGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGAt                                                                                  <  1:4326061/101‑1 (MQ=255)


          tGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGAt                                                                                  >  2:398386/1‑101 (MQ=255)


          tGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGAt                                                                                  >  2:1048856/1‑101 (MQ=255)


           gCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATg                                                                                 <  1:3581104/101‑1 (MQ=255)


           gCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATg                                                                                 >  2:3880019/1‑101 (MQ=255)


              gACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGa                                                                              <  1:1977216/101‑1 (MQ=255)


                 gCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGc                                                                           >  2:3222519/1‑101 (MQ=255)


                 gCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGc                                                                           >  1:659770/1‑101 (MQ=255)


                 gCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCACCGAACCCCTACATGAAGGGCGATGCGAAGc                                                                           >  2:3449686/1‑101 (MQ=255)


                  cTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCt                                                                          <  1:4410095/101‑1 (MQ=255)


                   tGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTg                                                                         >  1:1597639/1‑101 (MQ=255)


                    gAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGa                                                                        >  2:3339256/1‑101 (MQ=255)


                    gAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGa                                                                        >  2:4405878/1‑101 (MQ=255)


                    gAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGa                                                                        >  1:2798230/1‑101 (MQ=255)


                    gAAAGCCGATCAGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGa                                                                        >  2:4385935/1‑101 (MQ=255)


                     aaaGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAg                                                                       <  2:3065803/101‑1 (MQ=255)


                     aaaGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAg                                                                       <  2:79791/101‑1 (MQ=255)


                      aaGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGc                                                                      >  2:2744007/1‑101 (MQ=255)


                       aGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCt                                                                     >  1:1685459/1‑101 (MQ=255)


                       aGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCt                                                                     >  1:4351442/1‑101 (MQ=255)


                       aGCAGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCt                                                                     >  2:4366751/1‑101 (MQ=255)


                        gCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTa                                                                    <  2:4328410/101‑1 (MQ=255)


                          cGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATg                                                                  >  1:626688/1‑101 (MQ=255)


                           gATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGt                                                                 >  2:13625/1‑101 (MQ=255)


                           gATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGt                                                                 >  1:3695466/1‑101 (MQ=255)


                            aTCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTc                                                                >  1:3183916/1‑101 (MQ=255)


                            aTCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTc                                                                >  2:4684820/1‑101 (MQ=255)


                             tCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCg                                                               <  2:4038975/101‑1 (MQ=255)


                             tCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCg                                                               >  1:1033721/1‑101 (MQ=255)


                                gAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGca                                                            >  2:2642719/1‑101 (MQ=255)


                                gAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGca                                                            >  1:2078035/1‑101 (MQ=255)


                                gAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGca                                                            >  2:763864/1‑101 (MQ=255)


                                gAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGca                                                            <  1:122241/101‑1 (MQ=255)


                                gAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGca                                                            >  2:3478786/1‑101 (MQ=255)


                                 aaGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGTTGCGAAGCTGAGCTATGTCGGcac                                                           >  2:3278130/1‑101 (MQ=255)


                                 aaGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcac                                                           >  2:1453627/1‑101 (MQ=255)


                                 aaGGGCCTGACCCACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcac                                                           >  1:2577611/1‑101 (MQ=255)


                                  aGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcacc                                                          >  2:4288049/1‑101 (MQ=255)


                                   gggCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcacca                                                         <  2:4154019/101‑1 (MQ=255)


                                    ggCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcaccac                                                        >  1:695846/1‑101 (MQ=255)


                                    ggCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcaccac                                                        <  2:2565680/101‑1 (MQ=255)


                                    ggCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcaccac                                                        <  1:1778021/101‑1 (MQ=255)


                                      ccTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCt                                                      <  1:779245/101‑1 (MQ=255)


                                       cTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTc                                                     >  2:2283538/1‑101 (MQ=255)


                                        tGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCg                                                    <  2:3329434/101‑1 (MQ=255)


                                          aCCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGtt                                                  >  1:3361672/1‑101 (MQ=255)


                                          aCCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGtt                                                  >  2:297471/1‑101 (MQ=255)


                                          aCCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCCtt                                                  <  1:2710581/101‑1 (MQ=255)


                                           ccGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTc                                                 <  2:1597539/101‑1 (MQ=255)


                                            cGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCg                                                <  2:2015187/101‑1 (MQ=255)


                                             gACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCgg                                               >  2:117185/1‑101 (MQ=255)


                                              aCATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTcagatcgg                                              >  2:1815745/1‑94 (MQ=255)


                                              aCATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCggg                                              <  2:4126856/101‑1 (MQ=255)


                                               cATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGc                                             <  2:3519554/101‑1 (MQ=255)


                                               cATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGc                                             <  1:2601673/101‑1 (MQ=255)


                                                aTTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCa                                            <  1:2995323/101‑1 (MQ=255)


                                                aTTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCa                                            >  2:3961364/1‑101 (MQ=255)


                                                aTTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCa                                            >  2:51576/1‑101 (MQ=255)


                                                  tGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGa                                          >  1:238202/1‑101 (MQ=255)


                                                  tGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGa                                          <  1:557380/101‑1 (MQ=255)


                                                   gccgccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGaa                                         <  2:4540684/101‑1 (MQ=255)


                                                     cgccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGc                                       <  2:626624/101‑1 (MQ=255)


                                                     cgccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGc                                       <  2:2860826/101‑1 (MQ=255)


                                                           cAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGc                                 >  1:3292416/1‑101 (MQ=255)


                                                            aaGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAATGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCt                                >  1:912289/1‑101 (MQ=255)


                                                            aaGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCt                                <  2:4626542/101‑1 (MQ=255)


                                                               aCCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTc                             <  1:4518981/101‑1 (MQ=255)


                                                               aCCGCGATCGACAGGTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTc                             <  2:1072485/101‑1 (MQ=255)


                                                                  gcgATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCggg                          >  2:3098422/1‑100 (MQ=255)


                                                                   cgATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTg                         <  1:856392/101‑1 (MQ=255)


                                                                    gATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGc                        >  2:875384/1‑101 (MQ=255)


                                                                     aTCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGcc                       <  2:2926273/101‑1 (MQ=255)


                                                                     aTCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGcc                       <  2:2371842/101‑1 (MQ=255)


                                                                       cGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGc                     >  2:4313440/1‑101 (MQ=255)


                                                                        gACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGTGCAGAAGCGGCAGCTGTCGGTGCCGCt                    <  2:139368/101‑1 (MQ=255)


                                                                         aCAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTc                   <  1:402754/101‑1 (MQ=255)


                                                                           aGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgt                 <  2:1974471/101‑1 (MQ=255)


                                                                             tttCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtgg               >  2:2215450/1‑101 (MQ=255)


                                                                             tttCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtgg               <  1:134782/101‑1 (MQ=255)


                                                                              ttCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGGTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtggt              >  1:3309550/1‑101 (MQ=255)


                                                                                cgccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGa            <  2:2458412/101‑1 (MQ=255)


                                                                                    gcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGGGAACGa        >  1:2259446/1‑101 (MQ=255)


                                                                                      gccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAAt      <  2:655920/101‑1 (MQ=255)


                                                                                        cgAACCCCTACATGAAGGGCGATGCGACGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGGGCCGCTCGTGGTGAACGAATAc    >  1:2362911/1‑101 (MQ=255)


                                                                                        cgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATAc    <  1:1122916/101‑1 (MQ=255)


                                                                                        cgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATAc    <  1:296457/101‑1 (MQ=255)


                                                                                        cgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTAGTGGTGAACGAATAc    <  1:4543680/101‑1 (MQ=255)


                                                                                         gAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACa   <  1:3492256/101‑1 (MQ=255)


                                                                                         gAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACa   <  1:3880261/101‑1 (MQ=255)


                                                                                         gAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACa   >  2:1624015/1‑101 (MQ=255)


                                                                                          aaCCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACaa  >  1:1492192/1‑101 (MQ=255)


                                                                                          aaCCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACaa  >  2:2807672/1‑101 (MQ=255)


                                                                                          aaCCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGGGCGGGCCGGGGGGAACGAATACaa  >  2:2048768/1‑101 (MQ=255)


                                                                                          aaCCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGCCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCCCACGTGGCGAACAAATACaa  >  1:939473/1‑101 (MQ=255)

||


GAATATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCTAACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACAA  >  NC_014034/2620595‑2620785

Base quality scores:


ATCG

< 3 ≤


ATCG

< 23 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG