BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,166	Δ1 bp	2.9%	coding (48/1578 nt)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,166	0	C→.	2.9%	12.9 ‑0.8 ‑0.2	239	coding (48/1578 nt)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (2/5): ref base (106/126): total (108/131)
Fisher's exact test strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.27e-01

Only 100 of 287 total aligned reads displayed.


GCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1396066‑1396266


gcgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGc                                                                                                       >  2:4833388/1‑101 (MQ=255)


 cgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCg                                                                                                      <  1:3259368/101‑1 (MQ=255)


  gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGt                                                                                                     >  1:277148/1‑101 (MQ=255)


  gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGt                                                                                                     <  1:1689852/101‑1 (MQ=255)


   cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCCCTGCGTc                                                                                                    >  2:4518433/1‑101 (MQ=255)


   cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTc                                                                                                    >  1:3977461/1‑101 (MQ=255)


   cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTc                                                                                                    <  1:3856607/101‑1 (MQ=255)


   cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTc                                                                                                    <  2:502629/101‑1 (MQ=255)


        gATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcc                                                                                               >  2:2992327/1‑101 (MQ=255)


        gATGTATATGCCCGGTGAGACGGCTGTCGATCCCACCGCGGGGGCGTGGATCGAAAATTTTCGTC.ATCTGG.CAGGCTCGCGCCGGCACGATGAGTCGCTcc                                                                                               >  1:3731107/1‑101 (MQ=38)


         aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccc                                                                                              <  1:4523217/101‑1 (MQ=255)


           gTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccccc                                                                                            >  2:801945/1‑101 (MQ=255)


           gTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccccc                                                                                            >  1:1079077/1‑101 (MQ=255)


            tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                                           <  1:2210708/101‑1 (MQ=255)


            tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                                           <  2:3664271/101‑1 (MQ=255)


            tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                                           <  2:116247/101‑1 (MQ=255)


            tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                                           <  2:3866397/101‑1 (MQ=255)


            tataCGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                                           <  1:4204241/101‑1 (MQ=255)


             atatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCcg                                                                                          <  1:4675607/101‑1 (MQ=255)


               atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACCCTGCGCCGCTCCCCCcgcg                                                                                        >  1:1373032/1‑101 (MQ=255)


                  cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCgggg                                                                                     <  2:2479700/101‑1 (MQ=255)


                  cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCgggg                                                                                     <  2:2059802/101‑1 (MQ=255)


                    cGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGc                                                                                   <  2:2107058/101‑1 (MQ=255)


                       tgtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTg                                                                                <  2:10630/91‑1 (MQ=9)


                       tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCCGCGTCGCTCCCCCCCCGGGGGCggg                                                                                >  1:5053578/1‑99 (MQ=255)


                        gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                               >  1:1172676/10‑101 (MQ=2)


                        gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                               <  2:3446395/92‑1 (MQ=2)


                        gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                               <  1:4299983/101‑1 (MQ=255)


                        gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                               <  1:3828455/101‑1 (MQ=255)


                           aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                            <  1:3538387/101‑1 (MQ=255)


                               cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.aaa                                                                       >  1:4701924/1‑101 (MQ=255)


                                tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCCCGGGGGCGTGGGTCG.AAAc                                                                      >  1:962758/1‑101 (MQ=255)


                                tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCCCGGGGGCGTGGGTCG.AAAc                                                                      >  1:1018626/1‑101 (MQ=255)


                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAAccc                                                                    <  2:232097/101‑1 (MQ=255)


                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCg                                                                   >  1:3407938/1‑101 (MQ=255)


                                     cTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGGTGGCTGCCTCCCCCCGCGGGGGGGGGGACCA.TAACCCAGc                                                                 >  2:4578890/1‑101 (MQ=21)


                                      tCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCg                                                                <  1:581780/101‑1 (MQ=255)


                                        cccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaa                                                              >  2:3217798/1‑101 (MQ=255)


                                          cccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaaa                                                            <  1:4792169/101‑1 (MQ=255)


                                          cccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaaa                                                            <  2:3518471/101‑1 (MQ=255)


                                          cccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaaa                                                            <  2:911137/101‑1 (MQ=255)


                                           ccGCGGGGGCGTGGATCGAAACTTTTCGTG.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCCCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaaaa                                                           <  2:4157104/101‑1 (MQ=255)


                                           ccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAACCCGGccacca                                                            >  2:2375979/1‑96 (MQ=38)


                                            cgcgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAAc                                                          <  2:5047908/101‑1 (MQ=255)


                                             gcgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACg                                                         >  1:408899/1‑101 (MQ=255)


                                               gggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGc                                                       <  2:4460290/101‑1 (MQ=255)


                                                 gggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCa                                                     >  2:3021905/1‑101 (MQ=255)


                                                  ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCAt                                                    >  1:4400940/1‑101 (MQ=255)


                                                  ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCAt                                                    <  1:369663/101‑1 (MQ=255)


                                                   gCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATg                                                   <  1:4155837/101‑1 (MQ=255)


                                                    cGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAACCACGGCCATGc                                                  >  2:2465263/1‑101 (MQ=255)


                                                    cGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGc                                                  <  2:2932260/101‑1 (MQ=255)


                                                    cGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGc                                                  >  2:2592344/1‑101 (MQ=255)


                                                     gTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGcc                                                 <  2:188795/101‑1 (MQ=255)


                                                      tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCt                                                <  1:4424082/101‑1 (MQ=255)


                                                      tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAAACCGGCGAAAAACGGCCATGccc                                                >  2:1784672/1‑100 (MQ=255)


                                                       ggATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGATCG.AAACCCGGCGAAAAAAGGCCATGCCTc                                               >  2:719338/1‑101 (MQ=255)


                                                        gATCGAAACTTTTCTTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTcc                                              <  2:3730829/101‑1 (MQ=255)


                                                        gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAATGGCCATGCCTcc                                              <  1:2746338/101‑1 (MQ=255)


                                                        gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTcc                                              <  1:4481155/101‑1 (MQ=255)


                                                         aTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCg                                             <  1:2992555/101‑1 (MQ=255)


                                                           cGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCggg                                           >  2:4290259/1‑101 (MQ=255)


                                                           cGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCggg                                           >  1:3134901/1‑101 (MQ=255)


                                                            gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCcggg                                          >  2:4880280/1‑100 (MQ=255)


                                                               aCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTc                                       >  2:2559239/1‑101 (MQ=255)


                                                                cTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCt                                      >  1:2674950/1‑101 (MQ=255)


                                                                 ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg                                     >  1:2506927/1‑101 (MQ=255)


                                                                   ttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGc                                   <  2:277114/101‑1 (MQ=255)


                                                                   ttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGc                                   >  1:2761999/1‑101 (MQ=255)


                                                                      gTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGt                                <  1:7060/101‑1 (MQ=255)


                                                                       tC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTc                               <  2:1927243/101‑1 (MQ=255)


                                                                        c.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                              <  1:3674647/101‑1 (MQ=255)


                                                                          aTCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGc                             >  2:4737181/1‑101 (MQ=255)


                                                                            cTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTc                           <  2:3710130/101‑1 (MQ=255)


                                                                              gg.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccc                         >  1:3037596/1‑101 (MQ=255)


                                                                              gg.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccc                         >  2:43826/1‑101 (MQ=255)


                                                                               g.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccc                        <  2:859912/101‑1 (MQ=255)


                                                                                 cAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccccc                       <  2:2723375/101‑1 (MQ=255)


                                                                                 cAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccccc                       >  1:500605/1‑101 (MQ=255)


                                                                                  aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc                      <  1:1126323/101‑1 (MQ=255)


                                                                                  aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTCTGGCTGTCGCTcccccc                      >  2:1665496/1‑101 (MQ=255)


                                                                                   ggCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCcg                     >  1:1839494/1‑101 (MQ=255)


                                                                                    gCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCcgc                    <  1:888463/101‑1 (MQ=255)


                                                                                    gCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCcgc                    >  2:2200038/1‑101 (MQ=255)


                                                                                    gCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCcgc                    >  2:3317709/1‑101 (MQ=255)


                                                                                       cgcgCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCggg                 >  2:1476610/1‑101 (MQ=255)


                                                                                       cgcgCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCggg                 >  1:1781379/1‑101 (MQ=255)


                                                                                          gccgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGc              >  1:1323975/1‑101 (MQ=255)


                                                                                          gccgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGc              >  1:2334568/1‑101 (MQ=255)


                                                                                              ccACGGTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTgg          <  2:1636984/101‑1 (MQ=255)


                                                                                              ccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTgg          >  2:3078610/1‑101 (MQ=255)


                                                                                              ccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTgg          <  1:767385/101‑1 (MQ=255)


                                                                                                ccGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt        <  2:3183427/100‑1 (MQ=255)


                                                                                                aCGCTGCGTCGCACCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAGCGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGCGGCGTGGAt        <  1:838723/101‑1 (MQ=255)


                                                                                                  gCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCg      <  2:408834/101‑1 (MQ=255)


                                                                                                  gCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGCGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATca      >  2:579881/1‑100 (MQ=255)


                                                                                                     gCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa   >  1:3352184/1‑101 (MQ=255)


                                                                                                     gCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCAGGGTGTCTGGCTGTCGCTCCCACCGCGGGGGCGTGGATCGaga   >  2:4968186/1‑99 (MQ=255)


                                                                                                      cGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  <  1:2093564/101‑1 (MQ=255)


                                                                                                      cGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  <  1:4148995/101‑1 (MQ=255)


GCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1396066‑1396266

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG