BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,944	Δ1 bp	11.0%	coding (826/1578 nt)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,944	0	G→.	11.0%	138.8 ‑1.1 ‑1.5	408	coding (826/1578 nt)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (26/19): ref base (180/181): total (207/201)
Fisher's exact test strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.95e-02

Only 100 of 446 total aligned reads displayed.


CCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCC  >  NC_014034/1396844‑1397044


  ccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACA.GC.CT.GCGCG.Gc                                                                                                            >  2:300366/1‑101 (MQ=255)


     cgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGc                                                                                                        >  1:4157232/1‑101 (MQ=255)


      gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCa                                                                                                       >  1:2959220/1‑101 (MQ=255)


       ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..t                                                                                                    <  1:4668657/101‑1 (MQ=255)


         ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCg                                                                                                  >  2:4972547/1‑101 (MQ=255)


             tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCt.t                                                                                             >  1:4414915/1‑101 (MQ=255)


             tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCt.t                                                                                             >  2:958840/1‑101 (MQ=255)


             tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCt.t                                                                                             <  1:1484668/101‑1 (MQ=255)


              ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.Tc                                                                                            >  2:2689207/1‑101 (MQ=255)


              ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.Tc                                                                                            <  2:1460631/101‑1 (MQ=255)


                  cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGc.c                                                                                       >  1:4975406/1‑101 (MQ=255)


                     aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..c                                                                                  <  1:2043143/101‑1 (MQ=255)


                        cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTg                                                                               >  1:3662621/1‑101 (MQ=255)


                         ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTg.g                                                                             >  1:3779702/1‑101 (MQ=255)


                          gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CCG..CGTG.Gt                                                                            >  1:2327877/1‑101 (MQ=255)


                            ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TGCGC.CCG..CGTG.GTCg                                                                          >  2:2844613/1‑101 (MQ=255)


                              agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCt                                                                        >  1:1299497/1‑101 (MQ=255)


                                agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTcc                                                                      >  2:2929718/1‑101 (MQ=255)


                                agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTcc                                                                      <  1:2909133/101‑1 (MQ=255)


                                 gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GGCGCTccc                                                                     >  2:1724329/1‑101 (MQ=255)


                                  aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccc                                                                    >  2:1439239/1‑101 (MQ=255)


                                    agcccGCGCGGACCTCCTCAGACGTCGCCCCCCCCGCGTGGGCGTGGATCGAAACA.GC.CG.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTgccccc                                                                  <  2:225060/98‑1 (MQ=38)


                                    aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                  >  2:3407247/1‑101 (MQ=255)


                                     gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GGCGCTCCCCCcg                                                                 >  2:4440185/1‑101 (MQ=255)


                                      cccGCGCGGACCTCCTCAGGCGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCGCCCGCcgc                                                                <  2:1878481/100‑1 (MQ=38)


                                          cgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCgggg                                                            <  1:3991391/101‑1 (MQ=255)


                                              gccctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                                        <  2:3633955/99‑1 (MQ=255)


                                                 tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.GC.AGTG..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                      <  2:409452/92‑1 (MQ=0)


                                                 ggtcaacactgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                      <  2:3587270/91‑1 (MQ=9)


                                                  ccctcAGACGTCGCCCCCCCCGGGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGC.GGGGCGTGGATc                                                   <  2:41905/100‑1 (MQ=38)


                                                   tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  1:3217404/93‑1 (MQ=2)


                                                    cggttgcGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.GC.AGTG..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  1:1431807/95‑1 (MQ=2)


                                                    caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  2:2347530/94‑1 (MQ=9)


                                                     tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                 <  2:1290338/101‑1 (MQ=255)


                                                      ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                <  2:4591282/96‑1 (MQ=2)


                                                      cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                <  1:1845182/101‑1 (MQ=255)


                                                      cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                <  2:3157727/101‑1 (MQ=255)


                                                      acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                 >  1:4947849/6‑101 (MQ=9)


                                                       cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                               <  1:903933/97‑1 (MQ=2)


                                                       aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                               >  1:429884/1‑101 (MQ=255)


                                                        gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                              <  1:622073/101‑1 (MQ=255)


                                                        cccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                 <  1:4788053/99‑1 (MQ=2)


                                                         gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.GC.AGTG..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                              <  1:716399/100‑1 (MQ=2)


                                                         cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                             >  2:3058242/3‑100 (MQ=2)


                                                         cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                             <  2:4723137/99‑2 (MQ=2)


                                                          tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                             >  1:1780662/2‑100 (MQ=9)


                                                          cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                               <  1:2838087/101‑1 (MQ=2)


                                                          cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                               >  2:477790/1‑101 (MQ=2)


                                                          cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGGCGCTCCCCCCGCGGGGCCGTGGATCGaaa                                               >  2:1677022/1‑101 (MQ=2)


                                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.GC.AGTG..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                            <  2:1090364/101‑3 (MQ=2)


                                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATc                                           >  2:1004889/1‑101 (MQ=255)


                                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAActc                                            >  1:1057255/1‑99 (MQ=9)


                                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAActc                                            >  1:1664245/1‑99 (MQ=9)


                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                             >  1:4031554/1‑101 (MQ=2)


                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCa                                          >  1:3577883/1‑101 (MQ=255)


                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                          <  1:2667094/101‑1 (MQ=255)


                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                          >  1:4860951/1‑101 (MQ=255)


                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCACCGCGGGGGCGTGGATCGAAActaca                                          >  1:4238981/1‑97 (MQ=2)


                                                            tCGCTCCACCCGCGGGGTCGTGGGCCGAAACA.ACGCG.GCGCG.GG.CCTA..T.GGCTGTCGCC.CCC..CCCC.GTCGCTCCCCCCCCGGGGGCGTGGATCGAAActgc                                           <  2:710065/101‑4 (MQ=2)


                                                            tCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                          <  2:2644406/101‑1 (MQ=255)


                                                            gcGCGCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                          <  2:624089/100‑1 (MQ=255)


                                                            gcGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTTGATCGAAACATCa                                          <  2:2129897/100‑1 (MQ=255)


                                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                            >  1:1221739/1‑100 (MQ=2)


                                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAg                                         >  2:2427233/1‑101 (MQ=255)


                                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGTGCGTGGATCGAAActaaac                                         >  2:4009691/1‑96 (MQ=2)


                                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                          >  1:2176174/1‑97 (MQ=9)


                                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                           <  1:1913275/101‑3 (MQ=2)


                                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                        >  1:3393917/1‑95 (MQ=2)


                                                              ccTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                           <  2:3235949/100‑3 (MQ=2)


                                                              ccTCCCCCCGCGGGGGCGGGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCacc                                        <  2:4909765/100‑3 (MQ=255)


                                                                tCCCCCCGCGGGGGCGTGGATCGAAACA.CC.CG.GCGCC.GC.AGCG..AGTGCG.TCTGA.ACA..GGAT.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActatgctgg                                      >  2:2807018/1‑93 (MQ=2)


                                                                cccccccGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaa                                         <  1:223403/100‑2 (MQ=2)


                                                                cccccccGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                       <  1:4517929/100‑8 (MQ=9)


                                                                 ccccccGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                        <  1:1519451/101‑3 (MQ=2)


                                                                  cccccGCGGGGGCGTGGATCGAAACATGG.CTATGGCG.GC.AGTG..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgc                                     >  1:2290327/1‑92 (MQ=2)


                                                                  cccccGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                     <  1:2414911/101‑10 (MQ=9)


                                                                  cccccGCGGGGGCGTGGATCGAAAC..GC.TG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                     <  2:1579696/101‑10 (MQ=9)


                                                                   ccccGCGGGGGCGTGGATCGAAACT.GC.GT.GCGCG.CC.ATGAGTTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgc                                      <  2:4299223/101‑5 (MQ=0)


                                                                      cgcgGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                <  1:3333269/101‑1 (MQ=255)


                                                                      cgcgGGGGCGTGGAGCGAACCA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                <  2:799502/101‑1 (MQ=255)


                                                                       gcgGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                               >  1:3748730/1‑101 (MQ=255)


                                                                        cgGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                              >  1:639669/1‑101 (MQ=255)


                                                                          ggggCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAATCAGCTCGTCGTTGGTc                            >  1:3835259/1‑101 (MQ=255)


                                                                              cGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                        <  1:3496113/101‑1 (MQ=255)


                                                                               gTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTggacgccga                       >  1:2120748/1‑94 (MQ=255)


                                                                               gTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                       >  1:1970120/1‑101 (MQ=255)


                                                                               gTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCCTCGGTGGGCAGCCg                       >  2:4335797/1‑101 (MQ=255)


                                                                                      gAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                <  1:771636/101‑1 (MQ=255)


                                                                                      gAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                >  1:309201/1‑101 (MQ=255)


                                                                                      gAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                >  1:2536770/1‑101 (MQ=255)


                                                                                       aaaCA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt               >  1:1359769/1‑101 (MQ=255)


                                                                                         aCA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc             <  2:196544/101‑1 (MQ=255)


                                                                                             gC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc          <  1:2753155/101‑1 (MQ=255)


                                                                                                cT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc        >  2:3896688/1‑101 (MQ=255)


                                                                                                cT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc        >  1:4091246/1‑101 (MQ=255)


                                                                                                   gggcg.GC.CGCA..TCGGCT.TCCGC.CTG..CGGG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc      <  2:3748452/99‑1 (MQ=255)


                                                                                                   gcgcG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc      >  2:1358561/1‑101 (MQ=255)


                                                                                                     gcg.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccc    >  2:3328568/1‑101 (MQ=255)


                                                                                                       g.gC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc  >  2:121791/1‑101 (MQ=255)


                                                                                                       g.gC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc  >  2:1131640/1‑101 (MQ=255)


CCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCC  >  NC_014034/1396844‑1397044

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG