Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,699 Δ1 bp 2.0% intergenic (+3/+1895) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,6990G→. 2.0% 6.6 ‑1.8 ‑0.0244intergenic (+3/+1895)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (145/94):  total (146/98)
Fisher's exact test strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.96e-01

Only 100 of 261 total aligned reads displayed.

ATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGG  >  NC_014034/1397599‑1397799
                                                                                                    |                                                                                                    
   cgcgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTc                                                                                                   <  1:1229291/101‑1 (MQ=255)
     cgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTNTTCCGGTAACNNGNNNc                                                                                                 >  2:1308823/1‑101 (MQ=255)
     cgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGc                                                                                                 <  2:4998902/101‑1 (MQ=255)
      ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCt                                                                                                >  2:3978047/1‑101 (MQ=255)
      ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCt                                                                                                >  2:3451127/1‑101 (MQ=255)
      ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGCCTTGTTCTGTTCCGGCAACAGTTCGCt                                                                                                >  2:2429101/1‑101 (MQ=255)
           ggCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTccccc                                                                                           <  2:1036909/101‑1 (MQ=255)
           ggCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTccccc                                                                                           >  2:2016419/1‑101 (MQ=255)
           ggCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTccccc                                                                                           >  2:2597590/1‑101 (MQ=255)
             cGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCcg                                                                                         >  2:4047681/1‑101 (MQ=255)
               ggccccgccccgcCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCcgcg                                                                                       <  2:2458510/98‑1 (MQ=255)
                   ccgccccgccCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCggggg                                                                                   <  1:2050508/101‑3 (MQ=255)
                     gccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGggcg                                                                                 >  1:3430979/1‑101 (MQ=255)
                     gccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGggcg                                                                                 >  2:1739325/1‑101 (MQ=255)
                       cccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGNGCGTg                                                                               >  1:1000238/1‑101 (MQ=255)
                       cccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTg                                                                               >  2:651626/1‑101 (MQ=255)
                        ccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTgg                                                                              <  1:4050089/101‑1 (MQ=255)
                         cgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                             <  1:3622583/101‑1 (MQ=255)
                          gcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                            <  1:1783579/101‑1 (MQ=255)
                          gcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                            <  1:4104126/101‑1 (MQ=255)
                           cccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                           <  1:2173160/101‑1 (MQ=255)
                           cccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                           <  1:4609814/101‑1 (MQ=255)
                            ccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                          <  2:1992227/101‑1 (MQ=255)
                                 ggTCGCTCCCCCCGTGGGGGCGTGGATAGAAACTACATAGTTCCGCTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                     <  2:2490752/101‑1 (MQ=37)
                                 ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                     >  2:1749307/1‑101 (MQ=255)
                                      cTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCAGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCt                                                                <  2:4157297/101‑1 (MQ=255)
                                        ccccccGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGa                                                              <  1:2841096/101‑1 (MQ=255)
                                        ccccccGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGa                                                              <  1:3953712/101‑1 (MQ=255)
                                             cgcgGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACgcgc                                                         >  1:1421842/1‑101 (MQ=255)
                                              gcgGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCt                                                        >  2:954915/1‑101 (MQ=255)
                                               cgGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                                                       >  2:168613/1‑101 (MQ=255)
                                                ngnngNNTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcn                                                      <  1:1850660/100‑2 (MQ=255)
                                                gggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                                      >  2:4335759/1‑101 (MQ=255)
                                                gggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                                      <  2:4686915/101‑1 (MQ=255)
                                                gggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCACCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                                      >  1:4106228/1‑101 (MQ=255)
                                                 ggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcat                                                     <  2:4967708/101‑1 (MQ=255)
                                                  ggnCTTGGATCGAAACTNCAGAGTTCCGGTCTTGTTCTGTTCCGGTACCAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                                    <  2:1000128/101‑1 (MQ=255)
                                                  gggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                                    <  2:1867191/101‑1 (MQ=255)
                                                  gggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                                    <  1:3476601/101‑1 (MQ=255)
                                                    gCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAg                                                  <  1:3096963/101‑1 (MQ=255)
                                                      gTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCa                                                <  1:1739455/101‑1 (MQ=255)
                                                       tGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                                               <  1:223607/101‑1 (MQ=255)
                                                        ggATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                              >  2:4769965/1‑101 (MQ=255)
                                                        ggATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                              <  2:3684529/101‑1 (MQ=255)
                                                        ggATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                              >  1:4434546/1‑101 (MQ=255)
                                                        ggATCGAAACGACAGAGTTCCGGTCTTGTTCTGTGCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                              <  2:4264287/101‑1 (MQ=255)
                                                          aTCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTc                                            <  2:729019/101‑1 (MQ=255)
                                                           tCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCa                                           >  1:4764779/1‑101 (MQ=255)
                                                           tCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCa                                           <  1:2611475/101‑1 (MQ=255)
                                                             gAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg                                         <  2:1656463/101‑1 (MQ=255)
                                                              aaaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                        >  2:2726456/1‑101 (MQ=255)
                                                              aaaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                        <  1:836572/101‑1 (MQ=255)
                                                              aaaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                        >  1:1169007/1‑101 (MQ=255)
                                                               aaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAt                                       >  1:3788805/1‑101 (MQ=255)
                                                                aCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAtt                                      <  1:651715/101‑1 (MQ=255)
                                                                 cTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTc                                     <  2:2522704/101‑1 (MQ=255)
                                                                  tACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCa                                    >  2:3116790/1‑101 (MQ=255)
                                                                    cAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGt                                  >  2:1398491/1‑101 (MQ=255)
                                                                    cAGAGTTCCGGTCTTGGTCTGCTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGt                                  <  1:2486692/101‑1 (MQ=255)
                                                                     agagTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTc                                 <  1:1099327/101‑1 (MQ=255)
                                                                     agagTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAATc                                 >  2:1363793/1‑101 (MQ=255)
                                                                       aggtCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGc                               <  1:2131118/98‑1 (MQ=255)
                                                                       agTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGc                               >  2:4365197/1‑101 (MQ=255)
                                                                        gTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCt                              <  1:2720274/101‑1 (MQ=255)
                                                                         ttCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTc                             >  1:1580415/1‑101 (MQ=255)
                                                                          tCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGGCGCGCTCATCAGCACGTCAAGATGCAGTCGCTcc                            >  2:2205980/1‑101 (MQ=255)
                                                                           ccGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccc                           >  2:14924/1‑101 (MQ=255)
                                                                            nggNCNTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccc                          <  1:1308944/100‑1 (MQ=255)
                                                                            cGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccc                          <  2:2406678/101‑1 (MQ=255)
                                                                             ggTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCTTCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccccc                         <  1:4336076/101‑1 (MQ=255)
                                                                             ggTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccccc                         >  1:544108/1‑101 (MQ=255)
                                                                              gTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccccc                        <  1:3326575/101‑1 (MQ=255)
                                                                               tCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcg                       <  1:2554615/101‑1 (MQ=255)
                                                                               tCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcg                       <  2:3943236/101‑1 (MQ=255)
                                                                               tCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGGCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcg                       >  1:2396570/1‑101 (MQ=255)
                                                                                cTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcgc                      >  1:3656744/1‑101 (MQ=255)
                                                                                cTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcgc                      >  2:4803647/1‑101 (MQ=255)
                                                                                cTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcgc                      >  2:3540047/1‑101 (MQ=255)
                                                                                   gttctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCggg                   >  1:1340627/1‑101 (MQ=255)
                                                                                     tctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCggggg                 >  2:1167559/1‑101 (MQ=255)
                                                                                      ctgttcCGGTAACAGGTCGGTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc                <  2:212962/101‑1 (MQ=255)
                                                                                      ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc                >  2:2871769/1‑101 (MQ=255)
                                                                                      ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc                <  2:3356529/101‑1 (MQ=255)
                                                                                      ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc                >  1:588332/1‑101 (MQ=255)
                                                                                        gttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGt              <  1:4000871/101‑1 (MQ=255)
                                                                                             ggTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATc         >  1:3350063/1‑101 (MQ=255)
                                                                                             ggTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATc         >  2:28864/1‑101 (MQ=255)
                                                                                              gTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCg        >  1:943771/1‑101 (MQ=255)
                                                                                              gTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCg        >  2:1231522/1‑101 (MQ=255)
                                                                                               tAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa       >  1:4951145/1‑101 (MQ=255)
                                                                                               tAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa       >  1:1590727/1‑101 (MQ=255)
                                                                                                 aCAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa     <  2:3865971/101‑1 (MQ=255)
                                                                                                  cAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc    >  1:4514336/1‑101 (MQ=255)
                                                                                                  cAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc    >  2:2242571/1‑101 (MQ=255)
                                                                                                  cAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc    <  2:3084576/101‑1 (MQ=255)
                                                                                                  cAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCCGGGGCGAGGAACGAAAc    >  1:4209596/1‑101 (MQ=38)
                                                                                                   aGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct   >  1:878862/1‑100 (MQ=255)
                                                                                                   aGGTCGCTCCCCCCGCGGGGGCGTGAATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct   >  2:453965/1‑100 (MQ=255)
                                                                                                    ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc  <  2:2573392/101‑3 (MQ=255)
                                                                                                    ggTCGCTCCCCCCGCGGGGGCGTGGATAGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc  >  2:4939468/1‑99 (MQ=255)
                                                                                                    |                                                                                                    
ATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGG  >  NC_014034/1397599‑1397799

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG