Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,668,398	Δ2 bp	100%	intergenic (‑63/+18)	ilvH ← / ← ilvI	acetolactate synthase small subunit/acetolactate synthase large subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,668,398	0	A→.	100.0%	116.3	29	intergenic (‑63/+19)	ilvH/ilvI	acetolactate synthase small subunit/acetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (27/1):  ref base (1/0):  total (28/1)
*	NC_014034	1,668,399	0	G→.	100.0%	119.7	29	intergenic (‑64/+18)	ilvH/ilvI	acetolactate synthase small subunit/acetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (27/1):  ref base (1/0):  total (28/1)

GCTCGACGAGCCTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCAAGGGGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCGG  >  NC_014034/1668300‑1668490                                                                                                   ||                                                                                            gCTCGACAAGCCTTTCTTTATGTTCAGAGCGGCCATTGCCCTTTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCAGGGGGCAGgg                                                                                            >  2:2116355/1‑101 (MQ=38)                         cAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAgc                                                                  >  2:2368634/1‑101 (MQ=255)                          agagCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAgcg                                                                 >  2:23552/1‑101 (MQ=255)                                   aTTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGcgccgc                                                        >  2:1328317/1‑101 (MQ=255)                                           ctcCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAg                                                >  2:924356/1‑101 (MQ=255)                                                 tCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCg                                          >  2:4381544/1‑101 (MQ=255)                                                    gATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCa                                       >  2:2330049/1‑101 (MQ=255)                                                               aTCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGagag                            >  2:3106921/1‑101 (MQ=255)                                                               aTCTTGCCGGAAATATCCCGGGGGGCCCGGGGGGCA..GCGCCCCCCCGCCGCTCTCACACCAGCGCGGGGCCGCCGCGGAAGGACTCGGCGCCGGCAGagag                            >  2:1485245/1‑101 (MQ=18)                                                                tcgtGCCGGAAATATCCCGGGGGGTCGGGGGGGCA..GCCCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGagc                           <  2:3843969/98‑1 (MQ=37)                                                                tCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGagc                           >  2:3606292/1‑101 (MQ=255)                                                                 cTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGagca                          >  2:4808809/1‑101 (MQ=255)                                                                   tGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGagcagc                        >  2:1357110/1‑101 (MQ=255)                                                                   tGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCAAGggcacc                        >  2:5040593/1‑99 (MQ=25)                                                                    gCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAgcagca                       >  2:208626/1‑101 (MQ=255)                                                                     ccGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCAt                      >  2:518280/1‑101 (MQ=255)                                                                     ccGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCAt                      >  1:4194239/1‑101 (MQ=255)                                                                       ggAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCAttt                    >  1:1115157/1‑101 (MQ=255)                                                                       ggAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCCCCGCGGAAGGACTCGGCATCGGCCGAGAGCAGCAtgt                    >  2:5022072/1‑99 (MQ=37)                                                                           atatCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGtt                >  1:3490857/1‑101 (MQ=255)                                                                                 cGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCgg          >  1:4269358/1‑101 (MQ=255)                                                                                  ggggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGc         >  2:4309214/1‑101 (MQ=255)                                                                                  ggggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGc         >  2:3531874/1‑101 (MQ=255)                                                                                   gggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCt        >  2:4530376/1‑101 (MQ=255)                                                                                    ggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGcgt       >  2:4108937/1‑99 (MQ=38)                                                                                    ggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCtt       >  2:4302957/1‑101 (MQ=255)                                                                                     gggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTg      >  1:2991662/1‑101 (MQ=255)                                                                                       gTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGcc    >  1:3543814/1‑101 (MQ=255)                                                                                       gTCCGGGGGGCA..GAGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGcc    >  2:3032445/1‑101 (MQ=255)                                                                                       gTCCGGGGGGC.AGCGG.CCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGcc    >  1:4256820/1‑101 (MQ=255)                                                                                         ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCgg  >  1:839301/1‑101 (MQ=255)                                                                                                   ||                                                                                            GCTCGACGAGCCTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCAAGGGGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCGG  >  NC_014034/1668300‑1668490 Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG