BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,696,206	Δ1 bp	100%	intergenic (‑134/+91)	RCAP_rcc01564 ← / ← mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,696,206	0	G→.	100.0%	657.8	190	intergenic (‑134/+91)	RCAP_rcc01564/mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand): new base (87/101): ref base (2/0): total (89/101)

Only 100 of 196 total aligned reads displayed.


AATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTT.CGG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAG  >  NC_014034/1696110‑1696300


aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAAGgggaaa                                                                                           >  2:2295488/1‑98 (MQ=16)


 aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAac                                                                                        >  1:3210891/1‑101 (MQ=255)


 aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAac                                                                                        <  2:3649270/101‑1 (MQ=16)


  tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                                       <  1:345317/101‑2 (MQ=255)


  tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                                       <  1:4219830/101‑2 (MQ=255)


  tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                                       >  2:3228170/1‑100 (MQ=255)


   ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac                                                                                      <  1:3846889/101‑3 (MQ=255)


    tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                                     >  2:3136895/1‑98 (MQ=255)


    tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                                     >  2:2439141/1‑98 (MQ=255)


     cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.TGGCCCATTCCCCCCCGGAGGTTTGGGCCAAGGGGAAAAacct                                                                                      >  1:932033/1‑100 (MQ=255)


     cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                                    <  1:1251321/101‑5 (MQ=255)


     cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                                    >  1:4697230/1‑97 (MQ=255)


      gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                   <  2:4523873/101‑6 (MQ=255)


       aaTCCTTGCACGAATTTTGCAGGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                  >  1:2864404/1‑95 (MQ=255)


       aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                  <  2:5018585/101‑7 (MQ=12)


       aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                  <  1:3565055/101‑7 (MQ=12)


       aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                  >  1:3217323/1‑95 (MQ=255)


       aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctgg                                                                                  >  2:1390540/1‑95 (MQ=255)


         tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                                                <  2:1218947/101‑9 (MQ=255)


           cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                                              >  2:4579389/1‑91 (MQ=255)


              gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                         <  1:4274413/101‑1 (MQ=255)


              gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                         <  1:1407018/101‑1 (MQ=255)


                aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                       <  2:3719521/101‑1 (MQ=9)


                aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                       >  2:2193312/1‑101 (MQ=255)


                aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                       <  2:33411/101‑1 (MQ=9)


                 cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                                      <  2:595561/101‑1 (MQ=255)


                  gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                                     <  2:163841/101‑1 (MQ=255)


                  gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                                     <  1:3926370/101‑1 (MQ=255)


                     ttttGCCAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                                  <  2:1449166/101‑1 (MQ=255)


                      tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.TGGCCAATTCACCCCGGGATGGTTGGGCC.AAGGG.GAAA.CA.CCTTGCTTTTTCTTGGCGCa                                                                 >  1:2121468/1‑101 (MQ=255)


                      tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                                                                 >  2:2901065/1‑101 (MQ=255)


                      tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                                                                 <  2:1539810/101‑1 (MQ=9)


                       ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                                >  1:3350144/1‑101 (MQ=255)


                       ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                                >  2:4594879/1‑101 (MQ=255)


                         gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                              <  2:1904539/101‑1 (MQ=9)


                         gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                              <  1:3685381/101‑1 (MQ=9)


                          cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                             <  1:1637800/101‑1 (MQ=255)


                          cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                             >  2:355748/1‑101 (MQ=255)


                          cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                             <  2:4151953/101‑1 (MQ=255)


                           aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTATTTCTTGGCGCAAATAc                                                            >  2:4545305/1‑101 (MQ=9)


                            aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                                           <  2:3797522/101‑1 (MQ=9)


                            aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                                           <  1:2371661/101‑1 (MQ=9)


                             ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCCGC.CAATTCACCCCGGGATGTTTGGGCC.AAGGGGGAA..CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                          >  1:2698196/1‑101 (MQ=255)


                             ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCCGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAAGc                                                          >  1:4381474/1‑101 (MQ=9)


                              gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGcc                                                         <  1:2439315/101‑1 (MQ=255)


                              gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                         <  1:1932128/101‑1 (MQ=255)


                               aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                        >  2:3835162/1‑101 (MQ=12)


                               aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                        >  2:2769706/1‑101 (MQ=12)


                                ggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                                                       <  1:492532/101‑1 (MQ=255)


                                 gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                      >  1:1566168/1‑101 (MQ=9)


                                 gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                      <  2:2341899/101‑1 (MQ=255)


                                      agaggggcgctGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGc                                                   >  2:512860/11‑101 (MQ=9)


                                       gaggggcgctGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                                  >  2:3899256/10‑101 (MQ=16)


                                         ggggccctGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                                                <  1:3455533/94‑1 (MQ=255)


                                           ggcgctGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGaa                                              >  2:496754/6‑101 (MQ=16)


                                           ggcgctGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGaa                                              >  1:5002131/6‑101 (MQ=16)


                                               ctGCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                                          <  2:1858544/100‑1 (MQ=255)


                                                 gCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGTATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                        <  1:4301054/101‑1 (MQ=255)


                                                 gCCCCTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                        <  1:992890/101‑1 (MQ=255)


                                                  ccccTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                       <  2:1688056/101‑1 (MQ=19)


                                                  ccccTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                       <  2:1576724/101‑1 (MQ=255)


                                                  ccccTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                       >  2:4247008/1‑101 (MQ=19)


                                                   cccgcctGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc                                      <  2:2946894/95‑1 (MQ=16)


                                                    ccTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc                                     <  2:2373273/101‑1 (MQ=255)


                                                    ccTCCTGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc                                     <  2:3210640/101‑1 (MQ=255)


                                                      tcctGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgc                                   <  2:2073793/98‑1 (MQ=19)


                                                      tcctGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgc                                   >  1:370736/4‑101 (MQ=255)


                                                       cctGGCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGTCCCGAAGGGCAAgatcgg                                  >  1:4090752/3‑96 (MQ=255)


                                                          ggCCTTT.CGGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                               <  1:2164448/101‑1 (MQ=25)


                                                          ggCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                               >  2:3534139/1‑101 (MQ=255)


                                                          ggCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                               >  1:4154517/1‑101 (MQ=255)


                                                           gCCTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa                              <  1:2332952/101‑1 (MQ=19)


                                                            ccTTT.CGGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa                             <  1:1898918/101‑1 (MQ=25)


                                                            ccTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa                             >  2:4836454/1‑101 (MQ=255)


                                                            ccTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa                             >  1:1110783/1‑101 (MQ=255)


                                                            ccTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa                             <  2:2804073/101‑1 (MQ=19)


                                                             cTTT.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                            >  1:3159239/1‑101 (MQ=255)


                                                              ttt.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg                           <  2:1266650/101‑1 (MQ=19)


                                                               tt.CGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc                          >  1:3835587/1‑101 (MQ=255)


                                                                  cggccAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc                        <  1:2552609/97‑1 (MQ=25)


                                                                  cggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc                        <  1:4716805/97‑1 (MQ=25)


                                                                   ggccAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg                       <  2:3272317/98‑1 (MQ=25)


                                                                   ggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg                       >  2:692925/4‑101 (MQ=255)


                                                                    gccAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCgg                      <  1:4739985/99‑1 (MQ=25)


                                                                    gccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCgg                      >  2:3045488/3‑101 (MQ=255)


                                                                        attcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccc                  <  2:735126/95‑1 (MQ=255)


                                                                          tcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGAcccccc                >  1:2767977/5‑101 (MQ=25)


                                                                           caccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt               >  2:3310289/4‑101 (MQ=255)


                                                                            cccccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTg              <  1:610035/99‑1 (MQ=25)


                                                                               ccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATc           <  2:3916789/101‑1 (MQ=37)


                                                                               ccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATc           <  1:1139751/101‑1 (MQ=37)


                                                                               ccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTAGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGACCCCCTGATc           >  2:4759846/1‑101 (MQ=25)


                                                                                      gtttGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTc    <  1:2265746/99‑1 (MQ=37)


                                                                                      gtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTc    >  2:3484727/3‑101 (MQ=255)


                                                                                      gtttGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTc    <  2:2546887/99‑1 (MQ=37)


                                                                                      gtttGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTc    <  2:1996281/99‑1 (MQ=37)


                                                                                       tttGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCa   <  1:3025811/100‑1 (MQ=37)


                                                                                       tttGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCa   <  1:4836781/100‑1 (MQ=37)


                                                                                       tttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCa   >  1:2185681/2‑101 (MQ=255)


                                                                                        ttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAg  >  1:2935178/1‑101 (MQ=255)


AATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTT.CGG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAG  >  NC_014034/1696110‑1696300

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG