Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,225 Δ1 bp 100% coding (914/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,2250A→.100.0% 878.7 198coding (914/1314 nt)RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (88/110):  ref base (0/0):  total (88/110)

Only 100 of 212 total aligned reads displayed.

AGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGC  >  NC_014034/2180129‑2180322
                                                                                                   |                                                                                                 
aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                                                                                                 <  2:4873936/101‑3 (MQ=255)
 ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                                                                <  1:1132437/101‑4 (MQ=255)
 ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                                                                >  1:147550/1‑98 (MQ=255)
  gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                                                               <  1:2020329/101‑5 (MQ=255)
  gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                                                               <  2:2079819/101‑5 (MQ=255)
   acgcGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                                                                                             <  2:2382668/98‑1 (MQ=255)
   aCTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                                                                                             >  1:295099/1‑101 (MQ=255)
    cTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcgg                                                                                            <  1:3149737/101‑1 (MQ=255)
    cTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcgg                                                                                            <  1:651407/101‑1 (MQ=255)
     tCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggc                                                                                           <  2:1692278/101‑1 (MQ=255)
         ccGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                                                                       <  1:1891839/101‑1 (MQ=255)
         ccGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                                                                       <  1:1301638/101‑1 (MQ=255)
         ccGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                                                                       <  2:147534/101‑1 (MQ=255)
          cGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGACTGGAA.CCTCGCC.ATTCCGGCGGGcc                                                                                      >  2:2910233/1‑100 (MQ=255)
           gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                                                                     <  1:122408/101‑1 (MQ=255)
           gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                                                                     <  1:3242524/101‑1 (MQ=255)
            tGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACg                                                                                    <  1:2753416/101‑1 (MQ=255)
             ggCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGc                                                                                   >  1:3342451/1‑101 (MQ=255)
             ggCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGc                                                                                   >  1:890281/1‑101 (MQ=255)
             ggCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGc                                                                                   >  1:4215317/1‑101 (MQ=255)
              gCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                                                                  <  1:535428/101‑1 (MQ=255)
              gCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                                                                  <  2:116129/101‑1 (MQ=255)
               ccGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                                                                 <  1:4236434/101‑1 (MQ=255)
                cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa                                                                                <  2:726826/101‑1 (MQ=255)
                  aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                                                              <  1:811810/101‑2 (MQ=255)
                  aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                                                              >  2:4473588/1‑100 (MQ=255)
                    cATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                                                            >  2:3695777/1‑98 (MQ=255)
                    cATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                                                            >  2:5029536/1‑98 (MQ=255)
                     aTGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                           >  2:2753394/1‑97 (MQ=255)
                      tGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                                                         >  1:770619/1‑101 (MQ=255)
                      tGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                                                         <  2:928749/101‑1 (MQ=255)
                        tcggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg                                                                       <  2:191393/101‑1 (MQ=255)
                         cggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                                                                      >  1:1069435/1‑101 (MQ=255)
                          ggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                                                                     >  2:2231906/1‑101 (MQ=255)
                             ttcgcgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcggc                                                                  <  2:4384277/96‑1 (MQ=255)
                              tcgcgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGgcggcg                                                                 >  2:405501/5‑101 (MQ=255)
                                gcgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCt                                                               <  2:4617054/99‑1 (MQ=255)
                                   ctggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                                                            <  2:412797/95‑1 (MQ=255)
                                   ctcgggccGAACAAGGCCGGGGTCGCCAAGCATGGGAACTGGCTCTGGGCCTGTAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                                                            <  2:4431954/95‑1 (MQ=255)
                                    tggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa                                                           >  1:4540466/6‑101 (MQ=255)
                                      gcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAtt                                                         <  2:2193805/98‑1 (MQ=255)
                                      gcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAtt                                                         <  2:436819/98‑1 (MQ=255)
                                        gccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcg                                                       >  1:1549124/2‑101 (MQ=255)
                                          cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc                                                     <  1:737149/101‑1 (MQ=255)
                                           gAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGTCGAGGCGGCGCTGAAATtcgtcg                                                    <  1:4062236/101‑1 (MQ=255)
                                           gAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg                                                    <  2:3915830/101‑1 (MQ=255)
                                           gAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg                                                    >  1:2806325/1‑101 (MQ=255)
                                            aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                                   >  2:2338451/1‑101 (MQ=255)
                                            aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                                   <  1:3552808/101‑1 (MQ=255)
                                            aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                                   >  2:1434779/1‑101 (MQ=255)
                                            aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                                   >  2:1954314/1‑101 (MQ=255)
                                            aaCAAGGCCGGTGTCGCCAAGCATGGCAACCGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                                   >  1:378804/1‑101 (MQ=255)
                                             aCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGaa                                                  >  1:1695059/1‑101 (MQ=255)
                                              cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt                                                 <  1:2667471/101‑1 (MQ=255)
                                               aaggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATg                                                <  1:3696055/96‑1 (MQ=255)
                                                aggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg                                               <  2:4689622/97‑1 (MQ=255)
                                                aggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAGGCGGCGCTGAAATTCGTCGAATgg                                               >  2:3213435/5‑101 (MQ=255)
                                                   ccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCg                                            <  1:4172227/100‑1 (MQ=255)
                                                    cGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGa                                           >  2:2120952/1‑101 (MQ=255)
                                                     ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc                                          >  1:2348084/1‑101 (MQ=255)
                                                      gtgtCGCCAAGCATGGCAACTGGCTCTGGGACTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGTCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAcc                                         >  2:3386019/1‑101 (MQ=255)
                                                        gtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTc                                       >  2:3395861/6‑101 (MQ=255)
                                                         tcgccAAGTATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCTGCGCTGAAATTCGTCGAATGGGCGACCTcc                                      <  1:3626809/97‑1 (MQ=255)
                                                         tcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTcc                                      <  2:533213/97‑1 (MQ=255)
                                                         tcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTcc                                      <  2:1167721/97‑1 (MQ=255)
                                                          cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                                     <  2:3295267/98‑1 (MQ=255)
                                                          cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                                     <  1:1161580/98‑1 (MQ=255)
                                                          cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                                     <  1:388665/98‑1 (MQ=255)
                                                          cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                                     <  2:1221038/98‑1 (MQ=255)
                                                           gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                                    <  1:942706/99‑1 (MQ=255)
                                                           gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                                    >  1:964448/3‑101 (MQ=255)
                                                           cccAAGCATGGCAACTGGCTCTGGGCCTGG.ACCCTCGCC.ATTCCGGCGGGCACG.AAAAGGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                                    <  2:1548994/99‑1 (MQ=255)
                                                            ccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAg                                   <  2:4115676/100‑1 (MQ=255)
                                                            ccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAg                                   >  2:1092416/2‑101 (MQ=255)
                                                              aaGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGa                                 <  1:2264813/101‑1 (MQ=255)
                                                                gCATGGCAACTGGCTCTTGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACt                               <  2:4540146/101‑1 (MQ=255)
                                                                gCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACt                               <  1:2155953/101‑1 (MQ=255)
                                                                      cAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCg                         <  2:3552184/101‑1 (MQ=255)
                                                                      cAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCg                         >  1:4078428/1‑101 (MQ=255)
                                                                      cAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCg                         <  2:2863795/101‑1 (MQ=255)
                                                                       aaCTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGc                        <  1:1947994/101‑1 (MQ=255)
                                                                          tGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCt                     <  1:2166779/101‑1 (MQ=255)
                                                                          tGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCt                     >  2:798311/1‑101 (MQ=255)
                                                                           ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt                    >  2:2002479/1‑101 (MQ=255)
                                                                           ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCAACCTCCAAGGACTACACCGCCCtt                    >  2:3086346/1‑101 (MQ=255)
                                                                              tctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTc                 >  1:1892991/1‑101 (MQ=255)
                                                                              tctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTc                 <  1:4781046/101‑1 (MQ=255)
                                                                              tctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTc                 <  2:700825/101‑1 (MQ=255)
                                                                              tctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTc                 <  2:4546096/101‑1 (MQ=255)
                                                                               ctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGGCGAGGCGGGGCTGAAATTCGTCGAATGGGCGAACTCCAAGGGCTACACCGCCCTTGTCg                >  2:2654901/1‑101 (MQ=255)
                                                                                    ccTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGc           <  2:3817582/101‑1 (MQ=255)
                                                                                      tGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaa         >  1:3868490/1‑101 (MQ=255)
                                                                                      tGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaa         <  1:396220/101‑1 (MQ=255)
                                                                                      tGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaa         <  1:1040025/101‑1 (MQ=255)
                                                                                      tGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCCCCCTTGTCGCCAGCaa         >  1:2221598/1‑101 (MQ=255)
                                                                                        ggaaccTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaag        <  2:3759821/97‑1 (MQ=255)
                                                                                        ggaaccTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaag        >  2:2988144/5‑101 (MQ=255)
                                                                                        ggaaccTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaag        >  2:232442/5‑101 (MQ=255)
                                                                                         gaaccTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaagg       >  2:4727483/4‑101 (MQ=255)
                                                                                              tCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGc  >  2:3480256/1‑101 (MQ=255)
                                                                                                   |                                                                                                 
AGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGC  >  NC_014034/2180129‑2180322

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG