Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,474,914	Δ1 bp	100%	intergenic (‑77/+49)	RCAP_rcc02301 ← / ← engA	hypothetical protein/GTP‑binding protein EngA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,474,914	0	G→.	100.0%	142.6	40	intergenic (‑77/+49)	RCAP_rcc02301/engA	hypothetical protein/GTP‑binding protein EngA
Reads supporting (aligned to +/- strand):  new base (28/12):  ref base (0/0):  total (28/12)

GCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGGCGCAGCCCCCCGCGGGTCG.CCTCGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCA.GGCCCTTGTCCTTGCGTGCGGCG  >  NC_014034/2474814‑2475008                                                                                                     |                                                                                                  gCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCg                                                                                                   <  2:4708960/101‑1 (MQ=255)  cTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgg                                                                                                  <  1:3591830/101‑1 (MQ=255)    cccccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGACCGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgggg                                                                                                <  2:4372538/101‑1 (MQ=255)    cccccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGGgggg                                                                                                >  2:4013374/1‑101 (MQ=255)    cccccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgggg                                                                                                <  1:4744172/101‑1 (MQ=255)     ccccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggg                                                                                               <  1:4550087/101‑1 (MQ=255)      cccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgggggg                                                                                              <  1:2118997/101‑1 (MQ=255)       ccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggc                                                                                             <  2:1572461/101‑2 (MQ=255)       ccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggc                                                                                             <  1:2252796/101‑2 (MQ=255)          ggATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGAGGggggggggcc                                                                                          >  1:3837046/1‑98 (MQ=255)           gATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAg                                                                                        >  2:3531961/1‑101 (MQ=255)           gATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAg                                                                                        <  1:3611144/101‑1 (MQ=255)             tatTTCCCCCAAGGGGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTG.GGGGGGGCGCAGcc                                                                                      >  2:2105372/1‑101 (MQ=255)              atTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccc                                                                                     >  1:2923204/1‑101 (MQ=255)              atTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccc                                                                                     <  2:3764641/101‑1 (MQ=255)               tttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccc                                                                                    <  2:3213249/101‑1 (MQ=255)               tttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATAAGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccc                                                                                    <  1:986717/101‑1 (MQ=255)                ttgccccAAGGTGACAGGAAACCGCGGCCCCCCCCGCCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccccc                                                                                   <  2:2009495/98‑1 (MQ=255)                ttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccccc                                                                                   <  2:2237294/101‑1 (MQ=255)                ttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccccc                                                                                   <  1:2456304/101‑1 (MQ=255)                tccccccAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccccc                                                                                   <  2:3420516/99‑1 (MQ=255)                 tCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCCCCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccccc                                                                                  <  1:1019915/101‑1 (MQ=255)                 tCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccccc                                                                                  <  2:1111085/101‑1 (MQ=255)                 tCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccccc                                                                                  <  1:427311/101‑1 (MQ=255)                     ccAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCgg                                                                              >  2:17077/1‑101 (MQ=255)                       aaGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCgggg                                                                            >  1:3301542/1‑100 (MQ=255)                          gTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCG.c                                                                        >  1:1492822/1‑101 (MQ=255)                          gTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCG.c                                                                        >  1:393134/1‑101 (MQ=255)                            gACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTCggcc                                                                       >  2:80562/1‑98 (MQ=255)                               aGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCcggggcccgccc                                                                    >  2:952985/1‑92 (MQ=37)                                  aaaCCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCG.cccccgggc                                                                >  1:2925118/1‑94 (MQ=37)                                  aaaCCGCGGCCCACCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCG.CCCCGGGcg                                                                >  2:4794645/1‑100 (MQ=25)                                      cgcgGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGCCG.CCCCGGGGTTggc                                                            >  2:2053887/1‑99 (MQ=25)                                              cccccACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCG.CCTCCGGGCTGccccgggggg                                                    >  2:2225940/1‑94 (MQ=21)                                                 ccACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCG.CCCCCGGCGTGccccgagggggcc                                                 >  2:4480458/1‑91 (MQ=21)                                                        aaCGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCGCCCCCGGGCTTG.CCGGGGGGGCATCAG.cccc                                         >  1:2001505/1‑100 (MQ=18)                                                          cGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCG.CCCCCGGCTTGCCCGAGGCGACATCAG.CCCTTc                                       >  1:831406/1‑101 (MQ=25)                                                                  cTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTCG.CCCCGGGCTTGCCCCAGGCGGCACCAG.CCCTTctgctttcg                               >  2:616633/1‑93 (MQ=18)                                                                   ttttCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTCC.GCCCGGGGCTGCCCGCCGCGGCACCCG.CCCTTCgtcgtcccg                              >  2:4803743/1‑93 (MQ=12)                                                                     ttCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTCG.CCCCGGGCTTGCCCGCGGCGGCACACG.CCCTTCGGTTTCGCggg                            >  2:3781910/1‑101 (MQ=18)                                                                     ttCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCG.CCCCCGGCCTGGCCGCGGGGACCTCCGCCCCTCCGGTTTCGGgg                             >  2:2680818/1‑101 (MQ=12)                                                                       ccTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTCG.CCTCGGGCTTGCCCGAGGCGACATCAG.CCCCTCGGTTTCCCGGGCa                          >  1:869688/1‑101 (MQ=21)                                                                        cTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTCG.CCCCGGGCCTTCCCCCGGCGACAACAGCCCCTCCGGTTTTGGGGGcg                          >  2:226723/1‑100 (MQ=12)                                                                          tGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTCG.CCCCGGGTCTTCCCGAGGCGACATCAG.CCCCTCGGGTTCCAGGGCA.GGc                      >  2:5005974/1‑101 (MQ=18)                                                                              cAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCG.CCCCGGGGTTGCCCGCGGGGGCATCAG.CCCTTCGGGTTCGAGGGGA.GGcccct                  >  2:2698848/1‑99 (MQ=21)                                                                                   aCGCAGGGCGGGTGGTGC.GGGGGGCGCAGCCCCCCGCGGGTCG.CCTCGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCA.GGCCCTTGTCCt             <  2:1147926/101‑1 (MQ=255)                                                                                            ggggggTGC.GGGGGGCGCAGCCCCCCGCGGGGCG.CCCCGGGGTTTCCCGGGGGGGCCCCCG.CCCCTCGGGTTCGGGGGGAGGGCCCTGGTCCTT.CGTgcgg    >  1:1222979/1‑101 (MQ=2)                                                                                            ggggggTGC.GGGGGGCGCAGCCCCCCGCGGGGCG.CCCCGGGCTTGCCCCAGGCGACATCAG.CCCCTCGGGTTCGAGGGCA.GGCCCCTTTCCTTGCGGgcgg    >  2:2348999/1‑101 (MQ=18)                                                                                             gggggTGC.GGGGGGCGCAGCCCCCCGCGGGTCG.CCTCGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTTGAGGGCA.GGCCCCTGTCCTTGCGTgcggc   >  2:805226/1‑101 (MQ=255)                                                                                             gggggTGC.GGGGGGCGCAGCCCCCCGCGGGTCG.CCTCGGGCCTGCCCGAGGCGACATCAG.CCCCTCCGGTTCGAGGGCC.GGCCCCTGTCCTTGCGTgcggc   >  2:1516458/1‑101 (MQ=18)                                                                                              ggggTGC.GGGGGGCGCAGCCCCCCGCGGGTCG.CCTCGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCA.GGGCCTTGTCCTTGCGTgcggcg  >  2:3994164/1‑101 (MQ=255)                                                                                                     |                                                                                                  GCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGGCGCAGCCCCCCGCGGGTCG.CCTCGGGCTTGCCCGAGGCGACATCAG.CCCTTCGGTTTCGAGGGCA.GGCCCTTGTCCTTGCGTGCGGCG  >  NC_014034/2474814‑2475008 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG