Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,617,778 Δ1 bp 100% intergenic (‑152/‑462) RCAP_rcc02439 ← / → RCAP_rcc02440 hypothetical protein/AsnC/Lrp family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,617,7780C→.100.0% 29.1 182intergenic (‑152/‑462)RCAP_rcc02439/RCAP_rcc02440hypothetical protein/AsnC/Lrp family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (84/86):  ref base (4/8):  total (88/94)
Fisher's exact test strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.32e-08

Only 100 of 226 total aligned reads displayed.

GCGGGGATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTCCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAGA  >  NC_014034/2617678‑2617878
                                                                                                    |                                                                                                    
 gggggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcc                                                                                                     <  2:3455443/100‑1 (MQ=255)
  ggggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccc                                                                                                    >  1:546809/1‑101 (MQ=255)
    ggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccc                                                                                                  >  2:4773753/1‑101 (MQ=255)
    ggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccc                                                                                                  >  2:1532199/1‑101 (MQ=255)
     gATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcccccc                                                                                                 <  1:184386/101‑1 (MQ=255)
     gATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcccccc                                                                                                 >  2:1054625/1‑101 (MQ=255)
     gATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcccccc                                                                                                 <  2:2284819/101‑1 (MQ=255)
      aTCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccc                                                                                                <  1:937550/101‑1 (MQ=255)
       tCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcccccccc                                                                                               >  1:152789/1‑101 (MQ=255)
       tCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcccccccc                                                                                               <  2:4551134/101‑1 (MQ=255)
        cAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccg                                                                                              >  1:764915/1‑100 (MQ=255)
        cAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccg                                                                                              >  2:330277/1‑100 (MQ=255)
         aaGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccgg                                                                                             <  1:4374325/101‑3 (MQ=255)
           ggCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccggcc                                                                                           >  2:4812666/1‑97 (MQ=255)
           ggCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccggcc                                                                                           >  1:1663796/1‑97 (MQ=255)
             ccGGAATTCGGGCAGCCGACACGGCGCAGCGGCCAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCaa                                                                                        <  2:800688/101‑1 (MQ=255)
               ggAATTCGGGCCGCCGCCACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAg                                                                                      <  1:2227429/101‑1 (MQ=255)
                 aaTTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGcc                                                                                    >  1:2786859/1‑101 (MQ=255)
                  aTTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGccc                                                                                   <  2:1957389/101‑1 (MQ=255)
                  aTTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGccc                                                                                   >  2:1341902/1‑101 (MQ=255)
                   ttCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGcccc                                                                                  >  1:3011619/1‑101 (MQ=255)
                   ttCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGcccc                                                                                  <  2:4383447/101‑1 (MQ=255)
                     cGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCgg                                                                                <  2:2375768/101‑1 (MQ=255)
                      gggCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGc                                                                               <  1:1199556/101‑1 (MQ=255)
                        gCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGccc                                                                             <  1:2609414/101‑1 (MQ=255)
                         cAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCg                                                                            <  2:3309519/101‑1 (MQ=255)
                             cGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTc                                                                        <  1:355147/101‑1 (MQ=255)
                              gACACCGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCg                                                                       <  1:4979427/101‑1 (MQ=255)
                                cacaGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTc                                                                     >  2:4662505/1‑101 (MQ=255)
                                cacaGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTc                                                                     >  1:3345126/1‑101 (MQ=255)
                                cacaGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTc                                                                     <  2:3011391/101‑1 (MQ=255)
                                cacaGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTc                                                                     >  2:2550571/1‑101 (MQ=255)
                                 acaGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCt                                                                    >  1:1000851/1‑101 (MQ=255)
                                   aGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGt                                                                  <  1:1814975/101‑1 (MQ=255)
                                   aGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGt                                                                  <  1:4713829/101‑1 (MQ=255)
                                    gcgcAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTc                                                                 <  1:4839743/101‑1 (MQ=255)
                                    gcgcAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTc                                                                 <  1:242709/101‑1 (MQ=255)
                                          cTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTGCCGGCT.CCCCCCCCGGCCAAATCCCCGGCCCGACTCGTCTGTCgcggcg                                                           >  2:2088652/1‑101 (MQ=255)
                                            gctaGCCGCCGACGATGTCGAAATGCAGCGTGGCCAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGAc                                                         <  2:4255637/98‑1 (MQ=255)
                                            gACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAATCCCCCGCCCGACTCGTCTGTCGCGGCGAc                                                         >  2:841513/1‑101 (MQ=255)
                                            gACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGAc                                                         >  1:3797752/1‑101 (MQ=255)
                                               aGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGa                                                      >  2:1920392/1‑101 (MQ=255)
                                                 ccgccgACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGAtg                                                    >  2:762312/1‑101 (MQ=255)
                                                    ccgACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGCCTCGTCTGTCGCGGCGACggcggtgc                                                 >  2:1627071/1‑95 (MQ=255)
                                                     cgacgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTc                                                <  1:3982482/101‑1 (MQ=255)
                                                       gcgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCAt                                              <  2:1056171/100‑1 (MQ=255)
                                                       acgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCAt                                              >  1:3282410/1‑101 (MQ=255)
                                                       acgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCAt                                              <  2:125094/101‑1 (MQ=255)
                                                       acgaTGCCGAAATGCAGCGTGGACAGCGCCAGCGGCCCCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCAt                                              <  2:1889966/101‑1 (MQ=255)
                                                        cgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATg                                             <  1:2297889/101‑1 (MQ=255)
                                                        cgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATg                                             <  2:3237834/101‑1 (MQ=255)
                                                         gaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATgg                                            <  1:4812993/101‑1 (MQ=255)
                                                         gaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATgg                                            >  1:423964/1‑101 (MQ=255)
                                                         gaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGGGTCATgg                                            >  2:3785548/1‑101 (MQ=255)
                                                          aTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATggg                                           <  1:139071/101‑1 (MQ=255)
                                                            tttggaaagCAGCCTGGAAAGGCACAGCGGCCTCCCGGCT.CCCCCCCCGCCCAAAGCCCCGGCGCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCt                                         <  2:4940125/93‑1 (MQ=255)
                                                            gccagaaTGCAGCGGGGACGGGGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCATAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCt                                         <  2:4368599/96‑1 (MQ=255)
                                                            gTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCt                                         <  1:721114/101‑1 (MQ=255)
                                                              cGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTCCCCCCCCC.GCCAAAGCCCCGGCCCGGCGCGTCTGTCGCGGCGACGGATGTGGCATGGGCttt                                       >  1:2122562/1‑101 (MQ=255)
                                                              cGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCttt                                       <  2:4098567/101‑1 (MQ=255)
                                                              cGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCttt                                       <  1:4074130/101‑1 (MQ=255)
                                                              cGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCttt                                       >  2:2657327/1‑101 (MQ=255)
                                                               gcaaTGCAGCCTGGCCGGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTc                                      <  2:4954058/99‑1 (MQ=255)
                                                                aaaTGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCg                                     <  2:2923116/101‑1 (MQ=255)
                                                                aaaTGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCg                                     >  1:3114799/1‑101 (MQ=255)
                                                                aaaTGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCg                                     >  2:3984730/1‑101 (MQ=255)
                                                                 aaTGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGc                                    >  2:1607517/1‑101 (MQ=255)
                                                                  aTGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGCCTCGTCTGGCGCGGCGCCGGGTGTGTCATGGGGTTTCGCa                                   >  1:1965152/1‑101 (MQ=255)
                                                                  aTGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCa                                   >  2:17549/1‑101 (MQ=255)
                                                                    gCAGCGTGGACAGCGACAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGc                                 <  1:3517535/101‑1 (MQ=255)
                                                                      aGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCt                               <  1:4566424/101‑1 (MQ=255)
                                                                       gggttggcagcgacgacgGCTTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTc                              <  2:1413025/94‑1 (MQ=255)
                                                                       gCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTc                              <  2:494442/101‑1 (MQ=255)
                                                                        cggggACAGCGACAGCGGCCCCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTcc                             <  2:2013031/98‑1 (MQ=255)
                                                                        cGTGGACCGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTcc                             <  2:3273873/101‑1 (MQ=255)
                                                                         gTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTccc                            >  2:3556189/1‑101 (MQ=255)
                                                                         gTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTccc                            >  1:2492941/1‑101 (MQ=255)
                                                                           ggACAGCGCCAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGa                          <  1:1140148/101‑1 (MQ=255)
                                                                           ggACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGGTTTCGCAGCCTCCCGa                          >  2:4146666/1‑101 (MQ=255)
                                                                           ggACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGa                          >  2:407010/1‑101 (MQ=255)
                                                                           ggACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGa                          >  1:2752597/1‑101 (MQ=255)
                                                                                gcgacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTg                     <  1:3682771/101‑1 (MQ=255)
                                                                                 cgacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgc                    <  2:4573511/101‑1 (MQ=255)
                                                                                  gacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgcg                   >  2:3711621/1‑101 (MQ=255)
                                                                                  gacagcgGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgcg                   <  1:3324669/101‑1 (MQ=255)
                                                                                  gacagcgGCCCCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgcg                   <  2:2251309/101‑1 (MQ=255)
                                                                                     agcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGCCGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCaa                >  2:3505002/1‑101 (MQ=255)
                                                                                     agcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCaa                >  2:4727288/1‑101 (MQ=255)
                                                                                     agcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCaa                >  1:648376/1‑101 (MQ=255)
                                                                                     agcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGGGTCATGGGCTTTCGCAGCCTCCCGAAGGTGCGCaa                >  2:4623343/1‑101 (MQ=255)
                                                                                      gcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGGCGGATTGGTCATGGGCTTTCTGAGCCTCCCGCAGTTgcgtcac               >  1:3512407/1‑97 (MQ=255)
                                                                                      gcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAc               <  2:1171289/101‑1 (MQ=255)
                                                                                       cgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAcc              <  2:546730/101‑1 (MQ=255)
                                                                                        ggCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAccc             >  2:243093/1‑101 (MQ=255)
                                                                                        ggCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAccc             >  1:4426484/1‑101 (MQ=255)
                                                                                          ccccccGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCcgc           <  1:2333656/98‑1 (MQ=255)
                                                                                             cccGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGc        >  1:1894901/1‑101 (MQ=255)
                                                                                             cccGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGc        >  2:3050876/1‑101 (MQ=255)
                                                                                               ccggccccccccccGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCt       <  2:5025991/96‑1 (MQ=255)
                                                                                                    cccccccccGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAGa  <  2:4041820/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GCGGGGATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTCCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAGA  >  NC_014034/2617678‑2617878

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG