BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,725,840	Δ1 bp	2.9%	intergenic (‑112/+85)	cysT ← / ← cysK1	sulfate ABC transporter permease CysT/cysteine synthase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,725,840	0	C→.	2.9%	13.0 ‑0.8 ‑0.3	242	intergenic (‑112/+85)	cysT/cysK1	sulfate ABC transporter permease CysT/cysteine synthase
Reads supporting (aligned to +/- strand): new base (4/3): ref base (166/69): total (170/72)
Fisher's exact test strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.40e-01

Only 100 of 285 total aligned reads displayed.


TCCTTCCGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGGCGCCG  >  NC_014034/2725740‑2725940


tccttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.c                                                                                                       <  2:2612980/101‑1 (MQ=255)


 ccttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cc                                                                                                      >  2:267561/1‑101 (MQ=255)


  cttccGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.ccc                                                                                                     <  1:2342379/101‑1 (MQ=255)


      cGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.ccccccc                                                                                                 >  1:2964452/1‑101 (MQ=255)


       gTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cccccccc                                                                                                >  2:1590486/1‑101 (MQ=255)


       gTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cccccccc                                                                                                >  1:669073/1‑101 (MQ=255)


       gTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cccccccc                                                                                                <  2:1383535/101‑1 (MQ=255)


         gAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.cccccccccc                                                                                              >  2:814186/1‑101 (MQ=255)


          aGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCa                                                                                             <  2:4921667/101‑1 (MQ=255)


           gCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCAc                                                                                            <  1:2990614/101‑1 (MQ=255)


            cTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCCCt                                                                                           >  1:1665979/1‑101 (MQ=255)


              tCGTCATTTCCAAAAAATCCCCTGTGTCAGGCGAGTTTGAAAGGGATAAATTCCGCCATTCTTGGTTGTGAATTTAGAATTTTATT.CCCCCCCCCCCCTcc                                                                                         <  2:4526723/101‑1 (MQ=255)


               cGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTccc                                                                                        >  1:811608/1‑101 (MQ=255)


                gTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTcccc                                                                                       <  2:4240987/101‑1 (MQ=255)


                gTCATCTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATACTTGGTCGTGAACATAGAATTTTATTCCCCCCCCCCCACTccc                                                                                        >  1:341983/1‑101 (MQ=255)


                  cATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCg                                                                                     >  2:3373486/1‑101 (MQ=255)


                   aTTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCgg                                                                                    >  2:511951/1‑100 (MQ=255)


                   aTTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCgg                                                                                    >  2:3666338/1‑100 (MQ=255)


                      tCCAAAATATCCGCTGTGGCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTTAATATAAAATTTTATT.CCCCCCCCCCACTCCCCCGTGcc                                                                                 <  2:3201299/101‑1 (MQ=255)


                      nccAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCNCNNGnc                                                                                 >  1:439208/2‑101 (MQ=255)


                       ccAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCCTGCCg                                                                                >  1:2413520/1‑101 (MQ=255)


                         aaaaTATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGGGCCggg                                                                              >  2:4105942/1‑101 (MQ=255)


                          aaaTATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCgggg                                                                             >  2:4883328/1‑101 (MQ=255)


                           aaTATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCCCTCCCCCGTGCCggggg                                                                            >  1:189328/1‑100 (MQ=255)


                           aaTATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGgc                                                                            >  2:1963501/1‑101 (MQ=255)


                           aaTATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGgc                                                                            >  2:1103307/1‑101 (MQ=255)


                              atCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACCCCCCCGTGCCGGGGCGcc                                                                         >  1:3800919/1‑101 (MQ=255)


                                 cACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAgc                                                                      >  1:829089/1‑100 (MQ=255)


                                  aCTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.c                                                                    >  1:3380657/1‑101 (MQ=255)


                                   cTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.Cg                                                                   <  1:1590618/101‑1 (MQ=255)


                                     gtgtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGaa                                                                 >  2:1320545/1‑101 (MQ=255)


                                     gtgtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGaa                                                                 <  1:2039552/101‑1 (MQ=255)


                                      tgtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGcaa                                                                >  2:1544955/1‑101 (MQ=255)


                                      tgtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGaaa                                                                <  1:1520192/101‑1 (MQ=255)


                                       gtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGaaaa                                                               >  1:764054/1‑101 (MQ=255)


                                       gtCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGGGCCGGGGGCCCAGA.CGaaaa                                                               >  1:173643/1‑101 (MQ=255)


                                        tCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAg                                                              >  2:1089404/1‑101 (MQ=255)


                                         cAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCCCTCCCCCGTGCCGGGGCCCCAGA.CGAAAAgg                                                             >  2:1035710/1‑101 (MQ=255)


                                         cAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAgg                                                             >  1:3958086/1‑101 (MQ=255)


                                         cAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAgg                                                             <  1:4768553/101‑1 (MQ=255)


                                         cAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCACCCCCCCCGTCCCGGGGCGCCCGA.CGAAAGgg                                                             >  1:3730807/1‑101 (MQ=255)


                                            tCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGccc                                                          <  1:1369623/101‑1 (MQ=255)


                                              gagaTTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCccg                                                        >  2:3286859/1‑101 (MQ=255)


                                                gaTTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCccgcc                                                      >  2:4364102/1‑101 (MQ=255)


                                                gaTTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCccgcc                                                      <  1:452498/101‑1 (MQ=255)


                                                gaTTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCccgcc                                                      <  2:4024739/101‑1 (MQ=255)


                                                 aTTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTAATT.CCCCCCCCCCCCCCCCCCGGCCCGGGCCCCCAAA.CG.AAAGGCCCCCCCgg                                                    >  2:3935933/1‑101 (MQ=255)


                                                  ttGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCgg                                                    <  2:763952/101‑1 (MQ=255)


                                                   tGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCggg                                                   >  1:3555122/1‑101 (MQ=255)


                                                     aaaGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCggggg                                                 >  1:1984748/1‑101 (MQ=255)


                                                       aGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTCCCGGGGCGCCAGA.CGAAAAGGCCCAGCCggggggg                                               >  1:631495/1‑100 (MQ=255)


                                                          gATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGggggcggg                                            >  2:1239810/1‑101 (MQ=255)


                                                          gATAAACCCCCCCATTCTTGGTCGGGAATATAAAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGggggcggg                                            <  1:2390295/101‑1 (MQ=255)


                                                            tAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGggggcggggc                                          <  1:267595/101‑1 (MQ=255)


                                                             aaaTCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGcc                                         <  2:3137298/101‑1 (MQ=255)


                                                             aaaTCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCC.CTCCCCCCGGCCCGGGGGCCCGACCAAAAAGGCCCCCCCGGGGGGGGGGGcc                                         >  1:981729/1‑101 (MQ=255)


                                                              aaTCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCt                                        >  2:3597293/1‑101 (MQ=255)


                                                              aaTCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT..CCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCtt                                       <  1:2799812/101‑1 (MQ=255)


                                                               aTCTCGCCATTTTTGGTCGTAAATATAGAATTTTTTC.CCCCCCCCCCCCCCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCtt                                       <  1:4252243/101‑1 (MQ=255)


                                                               aTCTCGCCATTCTTGGTCTTTAATATAGAATTTTATT.CCCCCCCCCCACCCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCtt                                       <  1:4107697/101‑1 (MQ=255)


                                                               aTCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCtt                                       >  2:572150/1‑101 (MQ=255)


                                                               aTCTCCGCATTCTTGGGGCGGAAAATAGAATTTTTTC.CCCCCCCCCCACCCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCtt                                       <  2:3730529/101‑1 (MQ=255)


                                                                tctcGCCGTTCTTTGTCGGGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCCCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCttt                                      <  1:1301655/101‑1 (MQ=255)


                                                                 tcccccATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTg                                     <  2:2871323/97‑1 (MQ=255)


                                                                 ctcGCCATTCTTGGTCGTGAATATAGAATTTTATC.CCCCCCCCCCCCTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTg                                     <  1:1024987/101‑1 (MQ=255)


                                                                  tcGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCCCTCCCCCGTGCCGGGGCGCCCGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGa                                    >  2:2049181/1‑101 (MQ=255)


                                                                    gCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGaaa                                  >  2:250517/1‑101 (MQ=255)


                                                                     ccATTCTTGGTCGTGAATATAGAATTTTATT..CCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGa                                <  1:1132185/101‑1 (MQ=255)


                                                                      cATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGa                                >  1:3068303/1‑101 (MQ=255)


                                                                       aTTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGAc                               >  1:692617/1‑101 (MQ=255)


                                                                       aTTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGAc                               >  1:824832/1‑101 (MQ=255)


                                                                         tCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCCCTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGAcgc                             >  1:5019242/1‑101 (MQ=255)


                                                                         tCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGAcgc                             >  1:1691751/1‑101 (MQ=255)


                                                                         tCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTAAAAGAcgc                             >  2:1727498/1‑101 (MQ=255)


                                                                          cTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGAcgcg                            >  1:2453678/1‑101 (MQ=255)


                                                                          cTTGGTCGTGAATATAGAATTTTATC.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGAcgcg                            <  1:88479/101‑1 (MQ=255)


                                                                           ttGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCTCCGGGGGGCGGGGCCTTTGAAAGACGCgg                           >  1:2516619/1‑101 (MQ=255)


                                                                             ggTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCgggg                         >  2:1222645/1‑101 (MQ=255)


                                                                             ggTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCgggg                         >  1:2582932/1‑101 (MQ=255)


                                                                               tCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGTCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGaa                       >  2:2577684/1‑101 (MQ=255)


                                                                               tCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGGGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGAGGCGGGGaa                       >  2:2235503/1‑101 (MQ=255)


                                                                               tCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGCAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGaa                       >  2:4196891/1‑101 (MQ=255)


                                                                                 gggAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAAc                     <  2:2171165/99‑1 (MQ=255)


                                                                                 gTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAAc                     >  1:3075472/1‑101 (MQ=255)


                                                                                  ggAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAAcc                    <  1:4896383/100‑1 (MQ=255)


                                                                                   gAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCg                   >  1:4418510/1‑101 (MQ=255)


                                                                                   gAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCg                   <  2:668984/101‑1 (MQ=255)


                                                                                    aaTATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGa                  >  2:237511/1‑101 (MQ=255)


                                                                                      tataGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATc                <  1:3287121/101‑1 (MQ=255)


                                                                                      tataGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATc                >  2:4635285/1‑101 (MQ=255)


                                                                                       ataGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGGGCCAGA.CGAAAAGGCCCCCCCGGGGGGGGGGGCCTTTGAAAAACGCGGGGGAAACggcca               >  1:3491986/1‑97 (MQ=255)


                                                                                       ataGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGGAACCGATCa               >  2:3822614/1‑101 (MQ=255)


                                                                                       ataGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCa               >  1:1066789/1‑101 (MQ=255)


                                                                                         aGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAgg             >  1:2862228/1‑101 (MQ=255)


                                                                                         aGAATTTTATT.CCCACCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAgg             >  2:4668353/1‑101 (MQ=255)


                                                                                              tttATT.CGCCCCCCCCACTCCCCCCCGCGGGGCCCCGAGA.GGAAAGGGCCCCGCCGGGGGGCGCGGCCGTTGAAAGAGGCGGGGAAACCGAGATGGCCggg        >  2:2786219/1‑101 (MQ=255)


                                                                                               ttATG.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCgggg       <  1:1239927/101‑1 (MQ=255)


                                                                                                tATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGgc      >  1:1483643/1‑101 (MQ=255)


                                                                                                  tt.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGgcgc    >  2:4788461/1‑101 (MQ=255)


                                                                                                   t.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGGCGcc   <  1:4029151/101‑1 (MQ=255)


TCCTTCCGTGAGCTTCGTCATTTCCAAAATATCCACTGTGTCAGTCGAGATTGAAAGGGATAAATCTCGCCATTCTTGGTCGTGAATATAGAATTTTATT.CCCCCCCCCCACTCCCCCGTGCCGGGGCGCCAGA.CGAAAAGGCCCCGCCGGGGGGCGGGGCCTTTGAAAGACGCGGGGAAACCGATCAGGCCGGGGCGCCG  >  NC_014034/2725740‑2725940

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG