Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,926,430 Δ1 bp 98.3% coding (53/408 nt) RCAP_rcc02738 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,926,4300C→.98.3% 4.8 ‑3.8 ‑1.9238coding (53/408 nt)RCAP_rcc02738hypothetical protein
Reads supporting (aligned to +/- strand):  new base (88/146):  ref base (4/0):  total (92/146)
Fisher's exact test strand distribution p-value = 2.14e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.23e-02

Only 100 of 307 total aligned reads displayed.

TGAGCCGCGCATGGCCGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCTCCCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCGGATGCGACGGCCGAAG  >  NC_014034/2926330‑2926530
                                                                                                    |                                                                                                    
tGAGCCGCGCATGGCCGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCCAGCGTGCCGCCGACGTGTTCCCGGCGCTc                                                                                                      >  1:4288814/1‑101 (MQ=255)
  aGCCGCGCATGGCCGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCCCGCGGGCCGCCGACGTGTTCCGGGCGCTccc                                                                                                    >  1:4195957/1‑101 (MQ=255)
    ccGCGCATGGCCGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCTccccg                                                                                                  <  2:2475707/101‑2 (MQ=255)
        gcATGGCCGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGt                                                                                             <  2:2729918/101‑1 (MQ=255)
         cATGGCCGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGCGCGTGCCGCCGACGTGTTCCGGGCGCTccccgagggg                                                                                             >  2:2199265/1‑95 (MQ=255)
         cATGGCCGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTg                                                                                            <  2:1915318/101‑1 (MQ=255)
         cATGGCCGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGGGTTCCGGTCGCTccccgagggg                                                                                             >  1:2477747/1‑95 (MQ=255)
          aTGGCCGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCTccccgagggga                                                                                            >  1:417153/1‑94 (MQ=255)
          aTGGCCGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGa                                                                                           <  1:3981758/101‑1 (MQ=255)
           tGGCCGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCCCGCGTGCCGCCGACGTGTTCCGGGCGCTccccgaggggga                                                                                           >  2:1483132/1‑93 (MQ=255)
            ggCCGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAg                                                                                         <  1:493007/101‑1 (MQ=255)
            ggCCGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGGGCCGCCGCCGTGTTCCGGGCGCTCCCCC.AGGGGAAg                                                                                         >  1:1797916/1‑101 (MQ=255)
               cGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGCGGACCGGCTCCAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGGGAAGccc                                                                                      >  2:4765196/1‑100 (MQ=255)
               cGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGGGTGCCGCCGACGTGTTTCCGGCGCT.CCCCGAGGTGAAGCCg                                                                                      >  2:764572/1‑101 (MQ=255)
               cGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGGGAAGCCg                                                                                      >  1:3680303/1‑101 (MQ=255)
                  aCGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCCAGCGGGCCGCCCACGGGTTCCGGCCGCT.CCCCGAGGGGAAGCCGccg                                                                                   >  1:4403801/1‑99 (MQ=255)
                     tGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGt                                                                                <  1:4810802/101‑1 (MQ=255)
                     tGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGGGCCGCCGACGTGTTCCGGTCGCTCCCCC.AGGGGAAGCCCCCGGGt                                                                                >  1:1032570/1‑101 (MQ=255)
                      gAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGtt                                                                               <  2:3140720/101‑1 (MQ=255)
                      gAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCCGTCGCT.CCCCGAGGTGAAGCCGCAggggt                                                                               >  2:154016/1‑99 (MQ=255)
                        ggTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTAc                                                                             <  2:3005618/101‑1 (MQ=255)
                        ggTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTAc                                                                             <  2:869751/101‑1 (MQ=255)
                        ggTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTAc                                                                             <  1:2670412/101‑1 (MQ=255)
                         gTCGCAAATGGCTGTCTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACt                                                                            <  1:3074438/101‑1 (MQ=255)
                         gTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGGGAAGCCCCAGGGGTACt                                                                            >  1:4649807/1‑101 (MQ=255)
                           cGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTc                                                                          <  2:3223556/101‑1 (MQ=255)
                            gCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCTCCCCC.AGGTGAAGCCGCAGGGGTACTTCa                                                                         >  2:2927028/1‑101 (MQ=255)
                            gCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCa                                                                         <  2:2564504/101‑1 (MQ=255)
                             cAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGGGCGTGCCGCCGACGGGTTCCGGGCGCT.CCCCGAGGGGAAGCCGCCGGGGTACTTCaa                                                                        >  1:1139571/1‑101 (MQ=255)
                             cAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGGT.CCCCGAGGGGAAGCCGCAGGGTTACTTCaa                                                                        >  1:4541980/1‑101 (MQ=255)
                              aaaTGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCCAGCGTGCCGCCGACGGGTTCCCGTAGCT.CCCCGAGGGGAAGCCGCAGGGTTACTTCAAc                                                                       >  2:2870701/1‑101 (MQ=255)
                               aaTGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGGGAAGCCGCCGGGGTACTTCAAcg                                                                      >  2:2356936/1‑101 (MQ=255)
                                aTGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGGGAAGCCGCAGGGTTACTTCAAcgc                                                                     >  1:2396147/1‑101 (MQ=255)
                                 tGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAAcgcg                                                                    <  2:4162715/101‑1 (MQ=255)
                                  ggCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGGTACTTCAAcgcgg                                                                   >  1:2790222/1‑100 (MQ=255)
                                   gCTGCCGGAACGCTTGCCATGGTGGAGGACCGGCCCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTg                                                                  <  1:2341653/101‑1 (MQ=255)
                                      gactgaACGCTTGGTATGCTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGcc                                                               <  2:3387032/101‑1 (MQ=255)
                                      gactgaACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGGGAAGCCGCAGGGGTACTTCAACGCGTGGcc                                                               >  1:4591029/1‑101 (MQ=255)
                                        ctgaACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTg                                                             <  1:352686/101‑1 (MQ=255)
                                         tgaACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGa                                                            <  1:2431626/101‑1 (MQ=255)
                                         tgaACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGa                                                            <  1:1344891/101‑1 (MQ=255)
                                         tgaACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGGGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGa                                                            >  1:1563553/1‑101 (MQ=255)
                                          gaACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGGTCCGGTCGCT.CCCCGAGGGGAAGCCGCAGGGGTACTTCAACGCGTGGCCTggg                                                           >  1:2589481/1‑99 (MQ=255)
                                           aaCGCTTGCCATGGTCGAGGAGCGGCTCGAGAGTGCCGCCGACGTGTTCCGGGCGCT.CCCCGAGGGGAAGCCGCAGGGCTAGTTCAACGGGTGGCCTGCGt                                                          >  1:2875545/1‑101 (MQ=255)
                                           aaCGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGt                                                          <  1:3379581/101‑1 (MQ=255)
                                            aCGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTa                                                         <  1:189118/101‑1 (MQ=255)
                                            aCGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGGGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTa                                                         >  1:88179/1‑101 (MQ=255)
                                             cGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTAc                                                        <  1:2563207/101‑1 (MQ=255)
                                               cTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACtt                                                      <  1:4765519/101‑1 (MQ=255)
                                                ttGCCATGGTCGAGGACTGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTc                                                     <  2:4541660/101‑1 (MQ=255)
                                                 tGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTcc                                                    <  2:3769539/101‑1 (MQ=255)
                                                 tGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGGACTTcc                                                    >  2:402052/1‑101 (MQ=255)
                                                 tGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGGTACTTCAACGCCTGGCCTGAGTACTTcc                                                    >  2:2774309/1‑101 (MQ=255)
                                                  gCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGGTACTTCAACGCGTGGCCTGAGTACTTccc                                                   >  2:1837755/1‑100 (MQ=255)
                                                       ggTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCt                                              <  1:965480/101‑1 (MQ=255)
                                                        gTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCtt                                             <  2:632010/101‑1 (MQ=255)
                                                         gcGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTc                                            <  2:4190888/100‑1 (MQ=255)
                                                          cGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCg                                           <  2:167650/101‑1 (MQ=255)
                                                          cGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCg                                           >  2:3259043/1‑101 (MQ=255)
                                                          cGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCg                                           >  1:4112706/1‑101 (MQ=255)
                                                          cGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCg                                           >  2:4837389/1‑101 (MQ=255)
                                                           gAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGGTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGc                                          >  1:691576/1‑101 (MQ=255)
                                                            aGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGcc                                         <  1:1065292/101‑1 (MQ=255)
                                                             ggACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCg                                        <  2:1754244/101‑1 (MQ=255)
                                                             ggACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGGTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCg                                        >  1:3234383/1‑101 (MQ=255)
                                                               aCCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGAt                                      >  2:2609919/1‑101 (MQ=255)
                                                                ccGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATc                                     <  2:2395983/101‑1 (MQ=255)
                                                                ccGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATc                                     <  1:1955436/101‑1 (MQ=255)
                                                                 cggccggAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCa                                    <  2:2141889/95‑1 (MQ=255)
                                                                 cGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCa                                    <  1:2953475/101‑1 (MQ=255)
                                                                     tCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTc                                <  1:2291339/101‑1 (MQ=255)
                                                                     tCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTc                                <  2:4590553/101‑1 (MQ=255)
                                                                     tCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTc                                >  1:1281541/1‑101 (MQ=255)
                                                                     tCGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTc                                >  2:508845/1‑101 (MQ=255)
                                                                     tCGAGCGGTCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTc                                <  2:1216354/101‑1 (MQ=255)
                                                                      cGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCg                               >  1:3923349/1‑101 (MQ=255)
                                                                      cGAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCg                               <  2:428050/101‑1 (MQ=255)
                                                                       gAGCGTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCgg                              <  2:2695535/101‑1 (MQ=255)
                                                                           gTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAg                          <  2:493520/101‑1 (MQ=255)
                                                                           gTGCCGCCGACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAg                          >  1:345915/1‑101 (MQ=255)
                                                                               cgcccAGGTGTTCCGGTGGCT.CCCCGAGGCAAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTGCCACACCTTCGCCGATCAAGTCGGCCAGGAGc                      <  2:2259537/101‑1 (MQ=255)
                                                                                gccgACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGcc                     <  1:2363788/101‑1 (MQ=255)
                                                                                gccgACGTGTTCCGGTC.CCGCCCCGAGGTTAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACCCCTTCGCCGATCAAGTCGGCCAGGAGcc                     <  2:2858550/101‑1 (MQ=255)
                                                                                 ccgACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCt                    <  2:3559204/101‑1 (MQ=255)
                                                                                  cgACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTc                   <  1:1526271/101‑1 (MQ=255)
                                                                                   gACGTGTTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCg                  <  2:1516575/101‑1 (MQ=255)
                                                                                     gggggtCCGGCCGCT.CCCCGGGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCGGa                <  1:1484893/96‑1 (MQ=255)
                                                                                      gtgtTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCGGAt               >  1:608089/1‑101 (MQ=255)
                                                                                      gtgtTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCGGAt               <  2:3928913/101‑1 (MQ=255)
                                                                                      gtgtTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCGGAt               <  2:4684038/101‑1 (MQ=255)
                                                                                        gtTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCGGATGc             <  1:2048181/101‑1 (MQ=255)
                                                                                        gtTCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCGGATGc             >  2:3489397/1‑101 (MQ=255)
                                                                                         ttCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCGGATGCg            >  1:2109479/1‑101 (MQ=255)
                                                                                         ttCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCGGATGCg            <  2:1615367/101‑1 (MQ=255)
                                                                                         ttCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCGGATGCg            <  1:1770830/101‑1 (MQ=255)
                                                                                         ttCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGGCTGCCAGGAGCCGCGGCTGcc            >  2:567733/1‑100 (MQ=255)
                                                                                          tCCGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCGGATGCGa           >  2:1244142/1‑101 (MQ=255)
                                                                                           ccGGTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCGGATGCGAc          >  2:1320671/1‑101 (MQ=255)
                                                                                             ggTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCGGATGCGACgg        <  1:536564/101‑1 (MQ=255)
                                                                                              gTCGCT.CCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCGGATGCGACGGc       >  2:4380665/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TGAGCCGCGCATGGCCGAACGTGAGGTCGCAAATGGCTGACTGAACGCTTGCCATGGTCGAGGACCGGCTCGAGCGTGCCGCCGACGTGTTCCGGTCGCTCCCCCGAGGTGAAGCCGCAGGGTTACTTCAACGCGTGGCCTGAGTACTTCCACACCTTCGCCGATCAAGTCGGCCAGGAGCCTCGGATGCGACGGCCGAAG  >  NC_014034/2926330‑2926530

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG