Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,372 Δ1 bp 100% intergenic (‑82/+48) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3720G→.100.0% 144.4 138intergenic (‑82/+48)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (39/59):  ref base (31/8):  total (70/68)
Fisher's exact test strand distribution p-value = 2.62e-05
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.21e-01

Only 100 of 169 total aligned reads displayed.

GGAATTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCG  >  NC_014034/3461272‑3461460
                                                                                                    |                                                                                           
ggAATTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAaaggaag                                                                                             >  2:396433/1‑101 (MQ=255)
  aaTTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAggg                                                                                           <  2:66036/101‑1 (MQ=255)
   aTTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCATCCGCAGCTTAGCAGGATGCACGAGCGGGGCCAGATGCAGTTTGGCGCACCGCCAAAGGAAggg.g                                                                                         >  2:4694987/1‑101 (MQ=255)
   aTTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAggg.g                                                                                         >  1:2631798/1‑101 (MQ=255)
    tttCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAggg.gg                                                                                        >  2:1117869/1‑101 (MQ=255)
     ttCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAggg.ggc                                                                                       >  2:4574156/1‑100 (MQ=255)
     ttCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAggg.ggc                                                                                       >  1:527912/1‑100 (MQ=255)
      tCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAggg.ggcg                                                                                      >  1:1652618/1‑99 (MQ=255)
       cAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAggg.ggcgc                                                                                     <  1:2048328/101‑4 (MQ=255)
       cAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAggg.ggcgc                                                                                     >  1:2757978/1‑98 (MQ=255)
         gATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAggg.ggcgcag                                                                                   >  2:4781516/1‑96 (MQ=255)
         gATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAggg.ggcgcag                                                                                   <  1:1391404/101‑6 (MQ=255)
          aTCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGc                                                                                  >  1:3635541/1‑101 (MQ=255)
            cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGcca                                                                                <  1:1780175/101‑2 (MQ=255)
            cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGcca                                                                                >  1:428795/1‑100 (MQ=255)
            cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGcca                                                                                >  1:3238377/1‑100 (MQ=255)
              ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacc                                                                              >  1:1118659/1‑98 (MQ=255)
               tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccaccc                                                                             >  2:279776/1‑97 (MQ=255)
                cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccg                                                                            >  2:474418/1‑96 (MQ=255)
                cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccg                                                                            >  1:2370363/1‑96 (MQ=255)
                cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccg                                                                            >  2:1420045/1‑96 (MQ=255)
                cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccg                                                                            >  1:1921347/1‑96 (MQ=255)
                cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccg                                                                            >  1:1282849/1‑96 (MQ=255)
                 aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccgg                                                                           <  1:1853094/101‑7 (MQ=255)
                 aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccgg                                                                           >  1:3608169/1‑95 (MQ=255)
                  tCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccggc                                                                          >  1:1599206/1‑94 (MQ=255)
                   cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccggcc                                                                         >  1:1175180/1‑93 (MQ=255)
                   cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccggcc                                                                         <  1:4146014/101‑9 (MQ=255)
                   cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccggcc                                                                         >  2:1832181/1‑93 (MQ=255)
                     cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccggccgc                                                                       >  2:94584/1‑91 (MQ=255)
                                      aCCCGCAGCTTAGCAGGATGCGCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCg                                                >  1:2865036/1‑101 (MQ=255)
                                         cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                             <  1:2722614/101‑1 (MQ=255)
                                           cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                           >  2:1631519/1‑101 (MQ=255)
                                            agtgtAGCAGGAGGCAGGGGCGGTTGCAGACGAAGTTTGGCTCAAGGCCAAAGGAA.GG.GG.GCA..CG..GCCACCCGGCCGCGCCCCAAACCCCGGTCGTTCGAt                                          <  2:3521594/97‑1 (MQ=255)
                                            aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                          <  1:4779826/101‑1 (MQ=255)
                                             gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                         >  2:250744/1‑101 (MQ=255)
                                             gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                         >  2:1361351/1‑101 (MQ=255)
                                             gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                         >  1:1653085/1‑101 (MQ=255)
                                             gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                         >  1:591327/1‑101 (MQ=255)
                                              cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                        >  1:3617997/1‑101 (MQ=255)
                                               ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                       >  2:3886149/1‑101 (MQ=255)
                                               ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                       >  1:2141290/1‑101 (MQ=255)
                                                tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG.CCCC.CC.GGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                      <  2:1734556/101‑1 (MQ=255)
                                                tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                      >  1:4125168/1‑101 (MQ=255)
                                                tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                      <  1:448840/101‑1 (MQ=255)
                                                tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                      <  2:428730/101‑1 (MQ=255)
                                                   cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                   <  2:3163697/101‑1 (MQ=255)
                                                   cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                   <  2:689429/101‑1 (MQ=255)
                                                   cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGCACGTTCGATCAGTTCt                                   >  2:2290721/1‑101 (MQ=255)
                                                     ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                 >  1:936294/1‑101 (MQ=255)
                                                     ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                 <  1:4087209/101‑1 (MQ=255)
                                                      gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                <  2:497460/101‑1 (MQ=255)
                                                       aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                               <  2:1138371/101‑1 (MQ=255)
                                                       aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                               <  1:3823998/101‑1 (MQ=255)
                                                        tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                              <  2:3653283/101‑1 (MQ=255)
                                                        tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                              <  1:4738923/101‑1 (MQ=255)
                                                         gCAGGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAATGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcga                             <  1:4016918/101‑1 (MQ=255)
                                                          cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                            <  1:4916825/101‑1 (MQ=255)
                                                             gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                         <  2:399491/101‑1 (MQ=255)
                                                              ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                        <  1:1123647/101‑1 (MQ=255)
                                                               gcgcGGCCAGACGCAGTTGGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                       <  1:44086/101‑1 (MQ=255)
                                                                cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                      <  2:527833/101‑1 (MQ=255)
                                                                cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                      <  1:3113334/101‑1 (MQ=255)
                                                                  cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                    <  2:4577751/101‑1 (MQ=255)
                                                                  cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                    >  2:811604/1‑101 (MQ=255)
                                                                  cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                    <  1:2295233/101‑1 (MQ=255)
                                                                   ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                   <  1:2139702/101‑1 (MQ=255)
                                                                    gcaaGACGCGGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG.CCCC.CC.GGCCGCGCCCCCACCCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                  <  2:2275964/98‑1 (MQ=255)
                                                                    gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                  >  2:2728934/1‑101 (MQ=255)
                                                                    gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                  >  2:2904786/1‑101 (MQ=255)
                                                                     cctacctcAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                 <  1:3557390/94‑1 (MQ=255)
                                                                     ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                 <  2:1621888/101‑1 (MQ=255)
                                                                     ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                 <  2:1962496/101‑1 (MQ=255)
                                                                      cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                <  1:4166601/101‑1 (MQ=255)
                                                                       aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag               <  1:2759718/101‑1 (MQ=255)
                                                                        gACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg              <  2:3238126/101‑1 (MQ=255)
                                                                          cGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGcc            >  2:4475640/1‑101 (MQ=255)
                                                                           gCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt           >  2:3639907/1‑101 (MQ=255)
                                                                           gCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt           <  2:2697081/101‑1 (MQ=255)
                                                                           gCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt           <  1:721208/101‑1 (MQ=255)
                                                                           gCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt           <  2:4088210/101‑1 (MQ=255)
                                                                           gCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGCCGTTCGATCAGTTCTTGCCAACGATCACGTCAGAGACCt           >  2:3543420/1‑101 (MQ=255)
                                                                            cAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagagatcgg          >  2:3557120/1‑96 (MQ=255)
                                                                            cAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt          <  2:3431921/101‑1 (MQ=255)
                                                                            cAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTGGCCGACGATGACGTCAGAGGCCtt          >  2:4524917/1‑101 (MQ=255)
                                                                             attttGGCGGAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCGCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg         <  2:3072808/99‑1 (MQ=255)
                                                                             aGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg         <  2:4202124/101‑1 (MQ=255)
                                                                               tttGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat       <  1:838793/101‑1 (MQ=255)
                                                                               tttGGCGCAGGGCCAAAGG.ACGG.GG.GC.GCAG.CCCC.CC.GGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat       <  1:3233178/101‑1 (MQ=255)
                                                                                ttGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg      >  2:2005049/1‑101 (MQ=255)
                                                                                 tGGCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga     >  1:249448/1‑101 (MQ=255)
                                                                                 tGGCGCAGGGCCAAAGAAAGGGAGC.GCAGAC.ACCCCGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga     <  2:836350/101‑1 (MQ=255)
                                                                                  ggCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat    >  2:2984449/1‑101 (MQ=255)
                                                                                  ggCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat    >  2:3128474/1‑101 (MQ=255)
                                                                                  ggCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat    >  1:609573/1‑101 (MQ=255)
                                                                                   gcgcAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATc   >  2:716085/1‑101 (MQ=255)
                                                                                    cgcAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg  >  1:287405/1‑101 (MQ=255)
                                                                                    cgcAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg  <  1:4806283/101‑1 (MQ=255)
                                                                                    cgcAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg  <  2:2244752/101‑1 (MQ=255)
                                                                                    cgcAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg  <  1:2818690/101‑1 (MQ=255)
                                                                                                    |                                                                                           
GGAATTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCG  >  NC_014034/3461272‑3461460

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG