Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,379 Δ1 bp 100% intergenic (‑89/+41) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3790A→.100.0% 168.2 135intergenic (‑89/+41)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (37/60):  ref base (30/8):  total (67/68)
Fisher's exact test strand distribution p-value = 2.04e-05
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.85e-01

Only 100 of 142 total aligned reads displayed.

ATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCG  >  NC_014034/3461282‑3461460
                                                                                                  |                                                                                  
aTCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGc                                                                                  >  1:3635541/1‑101 (MQ=255)
 tCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGcc                                                                                 >  1:1622020/1‑101 (MQ=255)
  cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGcca                                                                                >  1:428795/1‑100 (MQ=255)
  cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGcca                                                                                >  1:3609291/1‑100 (MQ=255)
  cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGcca                                                                                >  1:3238377/1‑100 (MQ=255)
   tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccac                                                                               >  1:4202441/1‑99 (MQ=255)
    ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacc                                                                              >  1:1118659/1‑98 (MQ=255)
     tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccaccc                                                                             >  2:279776/1‑97 (MQ=255)
     tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccaccc                                                                             >  1:40898/1‑97 (MQ=255)
      cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccg                                                                            >  1:1282849/1‑96 (MQ=255)
      cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccg                                                                            >  2:4086906/1‑96 (MQ=255)
      cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccg                                                                            >  2:474418/1‑96 (MQ=255)
      cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccg                                                                            >  2:1420045/1‑96 (MQ=255)
      cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccg                                                                            <  1:4139660/101‑6 (MQ=255)
      cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccg                                                                            >  1:2370363/1‑96 (MQ=255)
       aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccgg                                                                           >  1:3608169/1‑95 (MQ=255)
       aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccgg                                                                           <  1:1853094/101‑7 (MQ=255)
        tCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggc                                                                          >  1:709200/1‑94 (MQ=255)
         cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggcc                                                                         >  1:589926/1‑93 (MQ=255)
         cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggcc                                                                         <  1:4146014/101‑9 (MQ=255)
         cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggcc                                                                         >  2:1832181/1‑93 (MQ=255)
         cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggcc                                                                         >  2:4166284/1‑93 (MQ=255)
         cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggcc                                                                         <  1:3525621/101‑9 (MQ=255)
          gCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggccg                                                                        <  1:1832314/101‑10 (MQ=255)
           cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggccgc                                                                       >  2:3232927/1‑91 (MQ=255)
           cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGccacccggccgc                                                                       >  2:94584/1‑91 (MQ=255)
                         cGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGGCGCAGCC.ACCCGGCCGCGCCCc.ccaccccgg                                                       >  2:2270533/1‑97 (MQ=255)
                            ccccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCg                                                <  2:4323307/100‑1 (MQ=255)
                            aCCCGCAGCTTAGCAGGATGCGCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCg                                                >  1:2865036/1‑101 (MQ=255)
                               cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                             <  1:3725713/101‑1 (MQ=255)
                               cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                             <  2:3880897/101‑1 (MQ=255)
                               cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                             <  1:2722614/101‑1 (MQ=255)
                                gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                            >  2:1769283/1‑101 (MQ=255)
                                 cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                           >  2:1631519/1‑101 (MQ=255)
                                 cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                           >  1:1141990/1‑101 (MQ=255)
                                  agtgtAGCAGGAGGCAGGGGCGGTTGCAGACGAAGTTTGGCTCAAGGCCAAAGGAA.GGGG.GCA..CG..GCCACCCGGCCGCGCCCCAAACCCCGGTCGTTCGAt                                          <  2:3521594/97‑1 (MQ=255)
                                  aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                          <  1:4779826/101‑1 (MQ=255)
                                   gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                         >  2:250744/1‑101 (MQ=255)
                                   gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                         >  2:1361351/1‑101 (MQ=255)
                                   gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                         >  1:591327/1‑101 (MQ=255)
                                   gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                         >  1:1653085/1‑101 (MQ=255)
                                     ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                       >  1:2141290/1‑101 (MQ=255)
                                      tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG.CCCC.CC.GGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                      <  2:1734556/101‑1 (MQ=255)
                                      tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                      <  1:448840/101‑1 (MQ=255)
                                      tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                      >  2:3011682/1‑101 (MQ=255)
                                         cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                   <  1:1276818/101‑1 (MQ=255)
                                         cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                   <  2:5034921/101‑1 (MQ=255)
                                         cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                   <  2:689429/101‑1 (MQ=255)
                                          aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                  <  2:3906992/101‑1 (MQ=255)
                                          aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                  <  2:4563316/101‑1 (MQ=255)
                                           ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                 >  1:936294/1‑101 (MQ=255)
                                           ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                 <  1:4087209/101‑1 (MQ=255)
                                            gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                <  2:2631613/101‑1 (MQ=255)
                                              tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                              <  2:3653283/101‑1 (MQ=255)
                                               gCAGGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAATGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcga                             <  1:4016918/101‑1 (MQ=255)
                                               gCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTCCGATCAGTTCTTGCcga                             >  1:2244907/1‑101 (MQ=255)
                                                cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                            <  1:4916825/101‑1 (MQ=255)
                                                  cGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacga                          <  1:2747186/101‑1 (MQ=255)
                                                   gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                         <  2:399491/101‑1 (MQ=255)
                                                    ggCGCGGCCAGACGCAGTTTGGTGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                        >  1:2047346/1‑101 (MQ=255)
                                                    ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                        <  1:1123647/101‑1 (MQ=255)
                                                     gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCTCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                       <  1:2442575/101‑1 (MQ=255)
                                                     gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                       >  1:4296095/1‑101 (MQ=255)
                                                      cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                      <  2:527833/101‑1 (MQ=255)
                                                      cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                      <  1:3113334/101‑1 (MQ=255)
                                                      cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                      <  2:4482703/101‑1 (MQ=255)
                                                      cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                      >  1:1688656/1‑101 (MQ=255)
                                                      cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                      <  1:474483/101‑1 (MQ=255)
                                                       gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                     <  2:3618306/101‑1 (MQ=255)
                                                        cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                    >  2:811604/1‑101 (MQ=255)
                                                        cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                    <  2:4577751/101‑1 (MQ=255)
                                                        cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                    <  1:2295233/101‑1 (MQ=255)
                                                          gcaaGACGCGGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG.CCCC.CC.GGCCGCGCCCCCACCCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                  <  2:2275964/98‑1 (MQ=255)
                                                          gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                  >  1:3224964/1‑101 (MQ=255)
                                                          gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                  >  2:2904786/1‑101 (MQ=255)
                                                          gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                  >  2:3094601/1‑101 (MQ=255)
                                                           cctacctcAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                 <  1:3557390/94‑1 (MQ=255)
                                                           ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                 <  2:1962496/101‑1 (MQ=255)
                                                           ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                 <  1:4579038/101‑1 (MQ=255)
                                                           ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                 <  2:1621888/101‑1 (MQ=255)
                                                             aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag               >  2:136277/1‑101 (MQ=255)
                                                             aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag               >  2:2783727/1‑101 (MQ=255)
                                                             aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag               <  1:2759718/101‑1 (MQ=255)
                                                              gACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg              >  2:941907/1‑101 (MQ=255)
                                                              gACGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg              <  2:3238126/101‑1 (MQ=255)
                                                                cGCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGcc            >  2:4475640/1‑101 (MQ=255)
                                                                 gCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt           <  1:721208/101‑1 (MQ=255)
                                                                 gCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt           <  2:2697081/101‑1 (MQ=255)
                                                                 gCAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGCCGTTCGATCAGTTCTTGCCAACGATCACGTCAGAGACCt           >  2:3543420/1‑101 (MQ=255)
                                                                  cAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagagatcgg          >  2:3557120/1‑96 (MQ=255)
                                                                  cAGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt          <  2:3431921/101‑1 (MQ=255)
                                                                   aGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg         <  2:4202124/101‑1 (MQ=255)
                                                                   aGTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg         <  2:819996/101‑1 (MQ=255)
                                                                    gTTTGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga        <  1:2103259/101‑1 (MQ=255)
                                                                     tttGGCGGAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat       <  2:4895966/101‑1 (MQ=255)
                                                                      ttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg      <  1:967084/101‑1 (MQ=255)
                                                                      ttGGCGCAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg      >  2:2005049/1‑101 (MQ=255)
                                                                        ggCGCAGGGCCAAAGGAAGGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat    >  2:3128474/1‑101 (MQ=255)
                                                                        ggCGCAGGGCCAAAGGAAGGGGGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat    >  2:2984449/1‑101 (MQ=255)
                                                                          cgcAGGGCCAAAGGAA.GGGG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg  <  1:2818690/101‑1 (MQ=255)
                                                                                                  |                                                                                  
ATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGGGG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCG  >  NC_014034/3461282‑3461460

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG