Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,464 Δ1 bp 2.0% intergenic (+67/+81) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4640G→. 2.0% 4.3 ‑2.0 ‑0.0203intergenic (+67/+81)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (70/128):  total (73/130)
Fisher's exact test strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.44e-01

Only 100 of 215 total aligned reads displayed.

CGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCG  >  NC_014034/432365‑432563
                                                                                                     |                                                                                                   
cgcCCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAg                                                                                                     <  1:2335154/101‑1 (MQ=255)
 gcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGc                                                                                                    <  1:1075139/101‑1 (MQ=255)
  cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                                                   <  2:826476/101‑1 (MQ=255)
  cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                                                   <  2:1746272/101‑1 (MQ=255)
   ccGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                                                                  <  1:953487/100‑1 (MQ=255)
   aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                                                                  <  2:2214106/101‑1 (MQ=255)
    cGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGa                                                                                                 <  2:128966/101‑1 (MQ=255)
      cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATc                                                                                               <  2:537867/101‑1 (MQ=255)
       tttCGGCGGGGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATcc                                                                                              <  1:4757869/101‑1 (MQ=255)
       tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATcc                                                                                              <  1:3375652/101‑1 (MQ=255)
       tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATcc                                                                                              <  1:1566945/101‑1 (MQ=255)
         tgggcggcgacggGTCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATccttt                                                                                           <  2:3230350/99‑4 (MQ=14)
             gggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                        <  1:2845949/100‑1 (MQ=255)
             cgggggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                        <  2:1351289/101‑1 (MQ=255)
             cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                        <  1:4086522/101‑1 (MQ=255)
              ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                                       <  1:1466521/101‑1 (MQ=255)
               gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                                      <  2:3441428/101‑1 (MQ=255)
                 ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                    <  1:639621/101‑1 (MQ=255)
                   ggggCCACGCCTGAGGCGGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                                                  <  1:1792129/100‑1 (MQ=255)
                   cggGCCACGCCTGAGGCCGGGCAGGGGGGCNCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                                                  <  1:1612793/101‑1 (MQ=255)
                    gggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCa                                                                                 <  2:2565931/101‑1 (MQ=255)
                    gggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCa                                                                                 <  1:1897214/101‑1 (MQ=255)
                     ggCCACGCCTGGGGCGGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                <  2:1073898/101‑1 (MQ=21)
                     ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                <  2:4950907/101‑1 (MQ=255)
                     ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                <  2:1607290/101‑1 (MQ=255)
                     ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                <  1:2017232/101‑1 (MQ=255)
                      gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                                               <  1:304608/101‑1 (MQ=255)
                           gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                                                          >  1:2418904/1‑101 (MQ=255)
                              tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                                                       >  2:2798863/1‑101 (MQ=255)
                               gAGGCCGGGCAGGGGGGCGCTGGCCCCCGTCC.TGCGGACTCCCCCCGGGACATTTGAGGCAAGATGAAAGCCGATCCGGGCTTAGTTTCATGTTGTCCCaa                                                                      >  2:4492459/1‑101 (MQ=18)
                               gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCaa                                                                      >  2:3227401/1‑101 (MQ=255)
                                aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCaaa                                                                     <  2:3022136/101‑1 (MQ=255)
                                 ggCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAat                                                                    <  1:1874169/101‑1 (MQ=255)
                                 ggCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAat                                                                    <  1:3858554/101‑1 (MQ=255)
                                  gCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAata                                                                   <  2:2672690/101‑1 (MQ=255)
                                  gCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAata                                                                   <  2:3530945/101‑1 (MQ=255)
                                  gCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAata                                                                   <  1:4501029/101‑1 (MQ=255)
                                  gCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAata                                                                   <  2:3795903/101‑1 (MQ=255)
                                  gCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAata                                                                   <  2:1032401/101‑1 (MQ=255)
                                   ccGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAatat                                                                  <  2:3246675/101‑1 (MQ=255)
                                   ccGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGAAATTTGAGGCAAGGTGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAattt                                                                  >  1:2452209/1‑99 (MQ=21)
                                    cGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATc                                                                 <  1:545878/101‑1 (MQ=255)
                                     gggCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATACcc                                                                >  2:4262044/1‑101 (MQ=255)
                                      ggCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATccc                                                               >  2:1125558/1‑101 (MQ=255)
                                       gCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCCTCTTGCCCCAAATATCCCg                                                              >  1:489739/1‑101 (MQ=255)
                                        acggggggCTCTGCCCCCCGG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttgg                                                  >  2:4596727/3‑99 (MQ=2)
                                         aGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGTTCCTTAAAACCAGATAAAAGGCCATACAGGCTTCGTTTCATCTTGCCCCCAAAATCCCggg                                                            >  1:4135910/1‑101 (MQ=9)
                                            ggggCGCTGCCCCCCGT.CGTGCGGACACCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCAGGGGt                                                         <  2:3379116/101‑1 (MQ=25)
                                             gggCGCTTCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                        <  2:4432724/101‑1 (MQ=255)
                                             gggCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                        <  2:3485013/101‑1 (MQ=255)
                                             gggCGCCGCCCCCCGTCC.TGCGGGCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                        <  1:3539895/101‑1 (MQ=25)
                                               gcgcTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGaa                                                      <  2:49757/101‑1 (MQ=255)
                                               gcgcTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGaa                                                      <  1:2744571/101‑1 (MQ=255)
                                                cgcTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                                                     <  2:4203244/101‑1 (MQ=255)
                                                 gcTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtt                                                    >  1:3449848/1‑101 (MQ=255)
                                                 gcTGCCCCCCGTCC.TGCGGACCCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATATTGCCCCAAATATCCCGGGGGTGAAtt                                                    <  1:4169243/101‑1 (MQ=37)
                                                  cTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                                                   <  2:54148/101‑1 (MQ=255)
                                                    gCCCCCCGTCC.TGCGGANTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg                                                 <  2:274773/101‑1 (MQ=255)
                                                     ccccccGTCC.TGCGGACTCCCCCGGGGGTATTTGACGCTACATGAAAGCCGATCAGGGCTTCGTTTCATCTTGCCCCAAATATACGGGGGGGGAAttttag                                                >  2:4041419/1‑99 (MQ=25)
                                                     ccccccGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTgg                                                <  1:4837390/101‑1 (MQ=255)
                                                      cccccGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCCAATCTCCCCGGGGCGAATTTTGgc                                               >  1:4017555/1‑101 (MQ=25)
                                                           gtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                          >  1:842326/4‑101 (MQ=255)
                                                           gtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                          >  1:3123025/4‑101 (MQ=255)
                                                             cc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCTGGGGTGAATTTTGGCGCAGCCa                                        <  2:123122/100‑1 (MQ=255)
                                                             cc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                        <  1:1758668/100‑1 (MQ=255)
                                                             cc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCa                                        >  1:3162226/2‑101 (MQ=255)
                                                                 gCGGACTCCCCCCGGGATATTTGAGGCAAGATGGGAGCCCATCCGGACCTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                     <  2:2452035/101‑1 (MQ=21)
                                                                  cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                                    <  1:4286309/101‑1 (MQ=255)
                                                                   ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga                                   >  2:3294030/1‑101 (MQ=255)
                                                                   ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAATTTTGGCGCaagatgaaac                                   >  1:4037511/1‑92 (MQ=25)
                                                                    gACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                  <  2:3149488/101‑1 (MQ=255)
                                                                        ccccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggggg                              <  2:4286450/101‑1 (MQ=255)
                                                                         cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                             <  2:4689836/101‑1 (MQ=255)
                                                                           cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                           <  2:3117522/101‑1 (MQ=255)
                                                                            ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                          <  1:1392253/101‑1 (MQ=255)
                                                                             cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                         >  1:2592007/1‑101 (MQ=255)
                                                                               ggATATTTGAGGCAGGATGAAAGCCGATCCGGGTTTCTTTCAATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc                       <  2:4110435/101‑1 (MQ=21)
                                                                               ggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc                       >  1:2912269/1‑101 (MQ=255)
                                                                                 atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                     <  2:3181778/101‑1 (MQ=255)
                                                                                  tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                    >  2:3748885/1‑101 (MQ=255)
                                                                                  tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                    <  2:3934374/101‑1 (MQ=255)
                                                                                  tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                    >  2:672938/1‑101 (MQ=255)
                                                                                    tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGGGCCCCCTa                  >  1:2642099/1‑101 (MQ=255)
                                                                                    tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                  >  2:3958219/1‑101 (MQ=255)
                                                                                    tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                  >  2:1482006/1‑101 (MQ=255)
                                                                                    tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                  >  1:90165/1‑101 (MQ=255)
                                                                                     ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                 <  1:2152761/101‑1 (MQ=255)
                                                                                     ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                 >  1:4457180/1‑101 (MQ=255)
                                                                                        aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc              <  2:5045661/101‑1 (MQ=255)
                                                                                        aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc              >  1:1269244/1‑101 (MQ=255)
                                                                                         ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc             <  1:3405425/101‑1 (MQ=255)
                                                                                          gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg            >  2:249247/1‑101 (MQ=255)
                                                                                          gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg            >  2:2031183/1‑101 (MQ=255)
                                                                                              gATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggc        >  1:4389669/1‑101 (MQ=255)
                                                                                               aTGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcg       >  1:4623905/1‑101 (MQ=255)
                                                                                                tGAAAGCCGATCCGTGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgc      >  1:2306212/1‑101 (MQ=255)
                                                                                                tGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgc      >  1:4986220/1‑101 (MQ=255)
                                                                                                 gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg     <  2:4212405/101‑1 (MQ=255)
                                                                                                 gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg     >  2:229648/1‑101 (MQ=255)
                                                                                                 gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg     >  2:795579/1‑101 (MQ=255)
                                                                                                     |                                                                                                   
CGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCG  >  NC_014034/432365‑432563

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG