Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,469 Δ1 bp 2.0% intergenic (+72/+76) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4690T→. 2.0% 3.8 ‑1.9 ‑0.1196intergenic (+72/+76)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (70/122):  total (73/123)
Fisher's exact test strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.82e-01

Only 100 of 212 total aligned reads displayed.

GCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.T.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGG  >  NC_014034/432370‑432569
                                                                                                     |                                                                                                     
 cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATc                                                                                                      <  1:142804/101‑1 (MQ=255)
  tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGCC.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGTTcc                                                                                                     <  1:3022955/101‑1 (MQ=21)
  tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATcc                                                                                                     <  1:4621467/101‑1 (MQ=255)
  tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATcc                                                                                                     <  2:457173/101‑1 (MQ=255)
   ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCg                                                                                                    <  1:414656/101‑1 (MQ=255)
    tgggcggcgacggGTCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATccttt                                                                                                  <  2:3230350/99‑4 (MQ=14)
    tCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCgg                                                                                                   <  1:4064213/101‑1 (MQ=255)
       gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGGAAGATGAAAGCCGATCCGGGcc                                                                                                >  1:368404/1‑100 (MQ=255)
        gggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                               <  1:2845949/100‑1 (MQ=255)
          gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                                             <  2:3441428/101‑1 (MQ=255)
           gggcggGCCACGCCTGAGGCCGGGCAAGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                                                            <  1:4238478/100‑1 (MQ=255)
           cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                                                            <  2:625054/101‑1 (MQ=255)
            ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                           <  2:4477121/101‑1 (MQ=255)
             gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                          <  1:755558/101‑1 (MQ=255)
              ggggCCACGCCTGAGGCGGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                                                         <  1:1792129/100‑1 (MQ=255)
               gggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCa                                                                                        <  2:2565931/101‑1 (MQ=255)
                ggCCACGCCTGGGGCGGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                       <  2:1073898/101‑1 (MQ=21)
                ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                       <  2:4950907/101‑1 (MQ=255)
                 gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                                                      <  2:334554/101‑1 (MQ=255)
                 gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                                                      <  2:1331622/101‑1 (MQ=255)
                  ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.t                                                                                    <  2:686553/101‑1 (MQ=255)
                   cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                                   <  1:867968/101‑1 (MQ=255)
                   cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                                   <  2:1607317/101‑1 (MQ=255)
                      gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGcc                                                                                <  1:3203470/101‑1 (MQ=255)
                         tGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCa                                                                             >  2:2798863/1‑101 (MQ=255)
                         tGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCa                                                                             <  1:1192728/101‑1 (MQ=255)
                         tGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCa                                                                             >  1:1954379/1‑101 (MQ=255)
                          gAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTTGGGCAAGATGAAAAACGATACCGGCGTCGATTCATCTTTGCCCCa                                                                             >  2:4402018/1‑101 (MQ=11)
                          gAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaa                                                                            >  2:3227401/1‑101 (MQ=255)
                          gAGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaa                                                                            <  1:2142989/101‑1 (MQ=255)
                           aGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaaa                                                                           <  2:394358/101‑1 (MQ=255)
                           aGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaaa                                                                           <  2:3022136/101‑1 (MQ=255)
                           aGGCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaaa                                                                           <  2:50344/101‑1 (MQ=255)
                            ggCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAat                                                                          <  1:1874169/101‑1 (MQ=255)
                             gCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                                                         <  2:3795903/101‑1 (MQ=255)
                             gCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                                                         <  2:3530945/101‑1 (MQ=255)
                             gCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                                                         <  2:2672690/101‑1 (MQ=255)
                             gCCGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                                                         <  1:245528/101‑1 (MQ=255)
                              ccGGGCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAatat                                                                        <  2:3246675/101‑1 (MQ=255)
                                gggCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATACcc                                                                      >  2:4262044/1‑101 (MQ=255)
                                 ggCAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATccc                                                                     >  2:1125558/1‑101 (MQ=255)
                                   cAGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCgg                                                                   <  2:1019912/101‑1 (MQ=255)
                                    aGGGGGGCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCggg                                                                  >  1:3138566/1‑101 (MQ=255)
                                       ggggCTCTGCCCCCCG.G.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttggccaa                                                    <  1:497130/101‑7 (MQ=2)
                                        gggCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTg                                                              <  2:3485013/101‑1 (MQ=255)
                                        gggCGCTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTg                                                              <  2:1553086/101‑1 (MQ=255)
                                          gcgcTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGaa                                                            <  1:1771721/101‑1 (MQ=255)
                                          gcgcTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGaa                                                            <  1:2744571/101‑1 (MQ=255)
                                          gcgcTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGaa                                                            <  2:49757/101‑1 (MQ=255)
                                          cgccgcccccccT.C.CTGCGGACCCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAt                                                           <  1:175571/95‑1 (MQ=25)
                                           cgcTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAt                                                           <  2:4203244/101‑1 (MQ=255)
                                           cgcTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAt                                                           <  1:2426842/101‑1 (MQ=255)
                                            gcTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAtt                                                          >  1:3449848/1‑101 (MQ=255)
                                             cTGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGAAttt                                                         >  1:4690159/1‑101 (MQ=255)
                                              tGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAtttt                                                        <  2:4046952/101‑1 (MQ=255)
                                              tGCCCCCCG.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAtttt                                                        <  2:3911819/101‑1 (MQ=255)
                                               gCCCCCCG.TCCTGCGGANTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTg                                                       <  2:274773/101‑1 (MQ=255)
                                                     ccgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCa                                                  <  1:2609886/96‑1 (MQ=255)
                                                      cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAg                                                 <  1:3959461/97‑1 (MQ=255)
                                                       gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                <  1:1579785/98‑1 (MQ=255)
                                                       gcccggCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                <  1:4402337/96‑1 (MQ=255)
                                                         ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCTGGGGTGAATTTTGGCGCAGCCa                                              <  2:123122/100‑1 (MQ=255)
                                                          cTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaa                                             <  1:534096/101‑1 (MQ=255)
                                                            gCGGACTCCCCCCGGGATATTTGAGGCAAGATGGGAGCCCATCCGGACCTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                           <  2:2452035/101‑1 (MQ=21)
                                                            gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGCATTTTGGCGCAGCCaaaa                                           >  2:4268636/1‑101 (MQ=255)
                                                             cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                                          <  1:4286309/101‑1 (MQ=255)
                                                             cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                                          <  1:906611/101‑1 (MQ=255)
                                                              ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga                                         >  2:3294030/1‑101 (MQ=255)
                                                               gACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                        <  2:3149488/101‑1 (MQ=255)
                                                                aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                       >  1:4763358/1‑101 (MQ=255)
                                                                 cTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggg                                      <  1:4896972/101‑1 (MQ=255)
                                                                    cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                                   <  2:540850/101‑1 (MQ=255)
                                                                     ctccGGGATTTTTGAGGCAAGATGAAAGCCGCTCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGCTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                  <  1:4492779/99‑1 (MQ=255)
                                                                      cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                 <  2:3117522/101‑1 (MQ=255)
                                                                        cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                               <  1:3099163/101‑1 (MQ=255)
                                                                           gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                            <  1:4113650/101‑1 (MQ=255)
                                                                           gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                            >  1:35106/1‑101 (MQ=255)
                                                                            atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                           >  2:510050/1‑101 (MQ=255)
                                                                            atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                           <  2:3181778/101‑1 (MQ=255)
                                                                            atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                           <  2:4017252/101‑1 (MQ=255)
                                                                             tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                          >  2:3748885/1‑101 (MQ=255)
                                                                              atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                         >  1:1357953/1‑101 (MQ=255)
                                                                              atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                         >  2:1380845/1‑101 (MQ=255)
                                                                              atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                         <  2:2366497/101‑1 (MQ=255)
                                                                                  gAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcc                     >  1:2899615/1‑101 (MQ=255)
                                                                                   nggNANNATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                    <  1:2332813/100‑1 (MQ=255)
                                                                                   aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                    >  1:2212981/1‑101 (MQ=255)
                                                                                    ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                   <  1:3405425/101‑1 (MQ=255)
                                                                                     gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                  >  2:249247/1‑101 (MQ=255)
                                                                                     gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                  >  1:2370646/1‑101 (MQ=255)
                                                                                          aTGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcg             >  2:4606091/1‑101 (MQ=255)
                                                                                            gAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg           <  2:4212405/101‑1 (MQ=255)
                                                                                             aaaGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg          >  1:1505094/1‑101 (MQ=255)
                                                                                             aaaGCCAACCCTCGCGTCGTTTCACC.TTGCCCAAAATATCCCGGGGGTGAATTGTGGCGCAGCCAAAAGAGGGGCCAGCCCCCCCTATCCCcgcggcgcgg          <  2:2037117/101‑1 (MQ=21)
                                                                                              aaGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc         >  1:4020882/1‑101 (MQ=255)
                                                                                              aaGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc         >  2:825711/1‑101 (MQ=255)
                                                                                               aGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGAGGCAGCGCCCCCTATCCCCgcggcgcggcg        >  1:56070/1‑101 (MQ=255)
                                                                                                  cGATCCGGGCATCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcg     >  1:4367326/1‑101 (MQ=255)
                                                                                                    aTCCGGGCTTGGTTTCATC.TTGCCCCAAATATCGGGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg   <  2:14305/101‑1 (MQ=255)
                                                                                                     tCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGcggcgcggcggcgcgg  >  1:2710468/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
GCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCG.T.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGG  >  NC_014034/432370‑432569

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG