Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014035	34,743	Δ1 bp	100%	coding (399/1041 nt)	RCAP_rcp00049 ←	iron siderophore/cobalamin ABC transporter permease

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014035	34,743	0	G→.	100.0%	126.0	55	coding (399/1041 nt)	RCAP_rcp00049	iron siderophore/cobalamin ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (29/25):  ref base (0/0):  total (29/26)

CGACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATGGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCGGCC  >  NC_014035/34643‑34839                                                                                                     |                                                                                                 cgACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATg                                                                                                  <  2:4378393/101‑1 (MQ=32) cgACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATg                                                                                                  <  1:4404867/101‑1 (MQ=32)  gACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgg                                                                                                 <  2:1736514/101‑1 (MQ=32)  gACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgg                                                                                                 >  2:4386391/1‑101 (MQ=32)  gACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgg                                                                                                 >  2:2394255/1‑101 (MQ=32)  gACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgg                                                                                                 >  2:3512060/1‑101 (MQ=32)   aCCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgga                                                                                                <  2:4793688/101‑2 (MQ=14)    cccccGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATggac                                                                                               <  1:4918574/101‑3 (MQ=11)    cccccGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATggac                                                                                               <  1:2842725/101‑3 (MQ=11)    cccccGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATggac                                                                                               <  1:347157/101‑3 (MQ=11)                       gTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACGAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGcc                                                                           >  2:2172224/1‑101 (MQ=2)                        ttCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCg                                                                          >  1:317583/1‑101 (MQ=14)                         tCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGa                                                                         >  1:4085652/1‑101 (MQ=17)                          cGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGAt                                                                        >  1:4099223/1‑101 (MQ=17)                           ggCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATg                                                                       <  2:2363389/101‑1 (MQ=17)                            gCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGc                                                                      >  2:1366940/1‑101 (MQ=17)                               cGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCa                                                                   >  2:4078369/1‑101 (MQ=17)                               cGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCa                                                                   <  2:2914522/101‑1 (MQ=17)                                 cTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGc                                                                 >  1:4330056/1‑101 (MQ=17)                                 cTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGc                                                                 >  1:469388/1‑101 (MQ=17)                                 cTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGc                                                                 >  2:1567197/1‑101 (MQ=17)                                  tGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCa                                                                >  1:3894881/1‑101 (MQ=17)                                   ggTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGGCCAGCAc                                                               >  2:1353053/1‑101 (MQ=12)                                   ggTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCAc                                                               <  2:4819473/101‑1 (MQ=17)                                     tCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGa                                                             >  1:312298/1‑101 (MQ=17)                                       gcgcCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATg                                                           <  2:3445500/101‑1 (MQ=17)                                       gcgcCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATg                                                           <  1:141011/101‑1 (MQ=17)                                        cgcCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGa                                                          <  1:78227/101‑1 (MQ=17)                                        cgcCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGa                                                          >  1:637335/1‑101 (MQ=17)                                          ccGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGAcc                                                        <  2:257895/101‑1 (MQ=17)                                          ccGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGAcc                                                        >  2:427266/1‑101 (MQ=18)                                            gTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGGCAGCCCGCCCGGCCGATGCCCAGCACGATGACCGc                                                      <  1:2062943/101‑1 (MQ=12)                                            gTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGc                                                      >  1:1820359/1‑101 (MQ=18)                                            gTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGc                                                      >  2:1090083/1‑101 (MQ=18)                                             ttCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGcg                                                     <  1:4981079/101‑2 (MQ=12)                                             ttCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGcc                                                     <  1:5032136/101‑1 (MQ=17)                                             ttCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGcc                                                     >  2:2442714/1‑101 (MQ=17)                                              tCGACAAAAGCGCGACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAA.CCCCCTAGGGTAAGCGACAGCCCGCCCGGCCGATGCCCAGCACAATGACCGCCa                                                    >  2:4185649/1‑101 (MQ=11)                                              tCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCa                                                    <  2:4085349/101‑1 (MQ=17)                                                gACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAcc                                                  <  2:5050496/101‑1 (MQ=17)                                                 aCAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcg                                                 >  2:3687011/1‑101 (MQ=18)                                                 aCAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcg                                                 <  2:3816559/101‑1 (MQ=17)                                                 aCAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcg                                                 <  1:3919473/101‑1 (MQ=17)                                                   aaaaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcgcg                                               >  2:3093434/1‑101 (MQ=17)                                                   aaaaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcgcg                                               <  2:2653571/101‑1 (MQ=17)                                                   aaaaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcgcg                                               >  1:418051/1‑101 (MQ=17)                                                    aaaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCgcgc                                              >  2:2119577/1‑101 (MQ=18)                                                     aaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGcc                                             <  2:2113254/101‑1 (MQ=17)                                                     aaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGcc                                             >  1:4902536/1‑101 (MQ=18)                                                     aaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGcc                                             <  2:3322170/101‑1 (MQ=17)                                                      aGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCg                                            >  1:1432167/1‑101 (MQ=18)                                                      aGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCg                                            <  1:4825952/101‑1 (MQ=17)                                                      aGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCg                                            <  1:517998/101‑1 (MQ=17)                                                       gcgcCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCgg                                           >  1:3207727/1‑101 (MQ=18)                                                       gcgcCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCgg                                           <  1:1292104/101‑1 (MQ=17)                                                        cgcCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGt                                          <  1:2276869/101‑1 (MQ=17)                                                          ccaccaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCg                                        >  1:4436042/1‑101 (MQ=17)                                                           caccaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGa                                       >  2:1442942/1‑101 (MQ=18)                                                            accaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAg                                      >  1:3056001/1‑101 (MQ=18)                                                                gcgcGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGccc                                  <  2:4668116/101‑1 (MQ=17)                                                                  gcgAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGccccc                                <  1:2419712/101‑1 (MQ=17)                                                                   cgAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCccg                               <  1:359565/101‑1 (MQ=17)                                                                     aaGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCccgcc                             >  1:2974142/1‑101 (MQ=17)                                                                      aGGCAGCGAAGGCGCCCGCGAAAGCCCCCA.AGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCccgccg                            <  1:4151104/101‑1 (MQ=12)                                                                          aGCGAAGGCGCCGGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCCCCGCGCCGGTCGAGGCCCCCGCCGAGAc                        <  2:482232/101‑1 (MQ=255)                                                                          aGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGAc                        >  1:284447/1‑101 (MQ=11)                                                                                         gAAAACCCCCATGGCA.GGCCACACCCCGGCCGGCGCAGCCCCAGCCCACTGCCCCCCCCCGCCCCGGTCGAGCCCCCCGCCGAGACGCCCAGCACGAAggg         <  1:3550950/101‑1 (MQ=2)                                                                                            aGCCCCCA.GGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCCCCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTc      <  1:4715970/101‑1 (MQ=12)                                                                                                 cccagggACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCGGcc  <  2:3539830/97‑1 (MQ=18)                                                                                                 cccagggACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCGGcc  <  1:1814032/97‑1 (MQ=18)                                                                                                     |                                                                                                 CGACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATGGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCGGCC  >  NC_014035/34643‑34839 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG