BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,255,985	+G	2.1%	intergenic (+155/+87)	RCAP_rcc01184 → / ← RCAP_rcc01185	CsbD family protein/methyl‑accepting chemotaxis sensory transducer

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,255,985	1	.→G	2.1%	4.8 ‑1.2 ‑0.1	188	intergenic (+155/+87)	RCAP_rcc01184/RCAP_rcc01185	CsbD family protein/methyl‑accepting chemotaxis sensory transducer
Reads supporting (aligned to +/- strand): new base (1/3): ref base (112/72): total (113/75)
Fisher's exact test strand distribution p-value = 3.03e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.89e-01

Only 100 of 223 total aligned reads displayed.


GCTGTGGTACCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.A.G.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGGCGCGC  >  NC_014034/1255895‑1256084


tgccccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.gggggggggg                                                                                               <  1:3275112/91‑1 (MQ=255)


ccccccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCCCCTTGGGCCAAATATCCC.gggggggggg                                                                                               <  2:4295401/91‑1 (MQ=21)


 gccccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGa                                                                                              <  2:829012/92‑1 (MQ=21)


 gccccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.ggggggggggg                                                                                              >  2:4410361/10‑100 (MQ=255)


 gccccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGa                                                                                              <  2:2940760/92‑1 (MQ=255)


 cccccccttcccTGAGGGTCTGCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGC.CTTTCACGTTGGGCCAAATTTCCC.GGGGGGGGGGa                                                                                              <  2:3371501/92‑1 (MQ=255)


  ccccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGaa                                                                                             <  1:732774/93‑1 (MQ=255)


   cccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGCAc                                                                                            >  2:3002205/8‑101 (MQ=255)


     cccctcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC..GGGGGGGGGAACTTc                                                                                         <  2:168078/96‑1 (MQ=255)


      ccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTc                                                                                         >  2:283333/5‑101 (MQ=255)


      ccgtcccTGCGGGACCCCCCCCGGGATATTTCCGCCAAGGGGAAATGACCCCGGGGCGCGG.CTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTc                                                                                         <  2:3441371/97‑1 (MQ=21)


         tcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCg                                                                                      <  2:4107180/100‑1 (MQ=255)


         tcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTcccc                                                                                      >  2:706835/2‑100 (MQ=255)


         tcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCg                                                                                      <  1:2168085/100‑1 (MQ=255)


         tcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC..GGGGGGGGGAACTTCCCgc                                                                                     <  2:3873504/100‑2 (MQ=255)


            ccgCGGGGCTACCCCCGGGAATTTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCCCCTTGGGCCAAAAATCCC.GGGGGGGGGGAACTTCCCg.a.a.g                                                                                <  2:1617438/99‑1 (MQ=255)


              gCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCg.a.a.gga                                                                              <  1:4794668/101‑3 (MQ=255)


                gggACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.a.a.g.gaaa                                                                           >  2:4637941/1‑100 (MQ=255)


                gggACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC..GGGGGGGGGAACTTCCCG.a.a.g.gaaag                                                                          <  2:4493995/101‑3 (MQ=255)


                gggACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC..GGGGGGGGGAACTTCCCG.a.a.g.gaaag                                                                          >  1:1257413/1‑99 (MQ=255)


                 ggcccccccccGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.a.a.g.gaaag                                                                          <  1:2698299/96‑3 (MQ=21)


                  gACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTGCCCG.a.a.g.gaaggg                                                                         >  2:4838232/1‑99 (MQ=255)


                    cTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAgg                                                                        <  1:695722/101‑1 (MQ=255)


                      ccccccGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAgggg                                                                      >  2:1926712/1‑101 (MQ=255)


                        ccccGGGATATTTCCGCCAAGGGGAAATTGACCCGGGGCGCGG.CTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAgggggg                                                                    <  1:2435760/101‑1 (MQ=37)


                         cccGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGc                                                                   <  1:3389400/101‑1 (MQ=255)


                         cccGGGATATTGCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGc                                                                   <  2:317945/101‑1 (MQ=255)


                            gggATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAgg                                                                <  2:2737199/101‑1 (MQ=255)


                              gATATTTCCGCCAAGGGGAAATGACCCCGGGGCGCGG.CTTTCACCTTGGGCCAAATAT.CC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGcc                                                             <  2:3309678/101‑1 (MQ=38)


                               atatTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGcc                                                             >  1:4122886/1‑101 (MQ=255)


                               atatTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC..GGGGGGGGGAACTTCCCG.A.AGG.AAAGGGGGGGGCAGGGcgc                                                            >  2:547406/1‑99 (MQ=25)


                                tatTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGccc                                                            >  2:3720944/1‑101 (MQ=255)


                                 atTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGcccc                                                           >  1:471185/1‑101 (MQ=255)


                                 atTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGcccc                                                           >  2:1339232/1‑101 (MQ=255)


                                 atTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGcccc                                                           >  1:1360551/1‑101 (MQ=255)


                                 atTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAAAGGGGGGCAGGGcccc                                                           >  1:375568/1‑101 (MQ=255)


                                 atTCCCGCAAGGGGGAAATGACCCCGGGGCGGGG.TTTTCCCCTTGGGCAAAATA.CCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGccccc                                                          <  1:3408631/101‑1 (MQ=255)


                                  tttCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGGACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGccccc                                                          >  1:2394673/1‑101 (MQ=255)


                                  ttccgatccaGGGGAAAGGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATCTCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGccccc                                                          <  1:3872338/92‑1 (MQ=37)


                                   ttCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCt                                                         <  2:2530271/101‑1 (MQ=255)


                                   ttCCGCCAAGGGAAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC..GGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCtt                                                        <  2:1838630/101‑1 (MQ=37)


                                    tCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCGGGGCCCCCtt                                                        >  1:1708599/1‑101 (MQ=255)


                                    tCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCtt                                                        >  2:382351/1‑101 (MQ=255)


                                      cGCCAGGGGGAAATGGACCCGGGGCGCGG.CTTTCCCCTGGGGCAAAATACCCG.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTc                                                      <  2:1021634/101‑1 (MQ=255)


                                      cGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTc                                                      >  2:1116261/1‑101 (MQ=255)


                                       gCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC..GGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTccc                                                    >  1:2627173/1‑101 (MQ=255)


                                        ccAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTccc                                                    >  2:383507/1‑101 (MQ=255)


                                        ccAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTccc                                                    >  1:2018828/1‑101 (MQ=255)


                                        ccAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCA.A.AGG.AAAGAGGGGGGAAGGGCCCCCTTTccc                                                    >  1:4167720/1‑101 (MQ=37)


                                         cAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCg                                                   >  1:164061/1‑101 (MQ=255)


                                           aGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGTA.G.G.AAAGAGGGGGGCAGGGGCCCCTTTCCCGAt                                                 >  1:4341818/1‑101 (MQ=37)


                                           aGGGAAAAGGACCCCGGGGCGCGG.CTTTCCCCTTGGGCCAAATATCCC.GGGGGGCGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGAt                                                 <  2:4362862/101‑1 (MQ=37)


                                             gggAAATGACCCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCg                                               <  2:4967118/101‑1 (MQ=255)


                                              ggAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCgg                                              >  1:4777263/1‑101 (MQ=255)


                                               gAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCggg                                             >  1:2270494/1‑101 (MQ=255)


                                               gAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCggg                                             >  1:1598558/1‑101 (MQ=255)


                                                gaaTGGACCCGGGGGCCGGTCTTTCCCCTTGGGCCAAATACCCG.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCggg                                             <  2:2394509/100‑1 (MQ=255)


                                                 aaTGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGc                                           >  1:4033825/1‑101 (MQ=255)


                                                   tGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGCAACTTCCCG.A.A.GCAAAAGGGGGGGCAGGGCCCCCTTTCCCGATCGGGTCCg                                         >  1:3014660/1‑101 (MQ=37)


                                                    gAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCgg                                        >  2:356091/1‑101 (MQ=255)


                                                    gAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCgg                                        >  2:2796475/1‑101 (MQ=255)


                                                        ccGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGGGcc                                    >  1:2574703/1‑101 (MQ=255)


                                                           gggCGCGG.CTTTCCCCTTGGGCCAAATATCCCGGGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCcgc                                  <  2:3512916/101‑1 (MQ=38)


                                                           gggCGCGG.CTTTCACCTTGGGCCAAATATCCCGGGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCcgc                                  >  1:145147/1‑101 (MQ=38)


                                                               gcgG.CTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCTGc                             <  2:145568/101‑1 (MQ=38)


                                                                ggg.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCa                            <  2:4885187/100‑1 (MQ=255)


                                                                 gg.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGGCGGTGCCGCGCGGCAg                           >  1:1510725/1‑101 (MQ=255)


                                                                 gg.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGGGCCGCGCGGCAg                           >  1:4664906/1‑101 (MQ=255)


                                                                  g.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGGACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCCCGGCAgg                          >  2:2405118/1‑101 (MQ=38)


                                                                  g.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGGCGGGCCCGCGCGGCAgg                          >  2:1544381/1‑101 (MQ=38)


                                                                  g.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTCTCCG.AGG.G.AAAGATGGGGGACGGACCCCCTTTCCCGATCGGGGACGGTGCGGCGCGGCGgg                          >  2:406216/1‑101 (MQ=21)


                                                                    cTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCGGGGCCCCCTTTCCCGATCGGGGCCGGGGCGGCCCGCCAggg                         >  2:3446519/1‑101 (MQ=38)


                                                                    cTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAggg                         >  1:1180404/1‑101 (MQ=255)


                                                                     tttCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGGGCCGCGCGGCAGGGt                        >  1:865124/1‑101 (MQ=255)


                                                                      ttCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTc                       <  2:4697719/101‑1 (MQ=255)


                                                                      ttCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTc                       >  1:2877048/1‑101 (MQ=255)


                                                                       tccccTGGGGCCAAATA.CCC.CGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCtt                     <  2:2264520/98‑1 (MQ=255)


                                                                       tCACCTTGGGCCAAATATCCC.GGGGGGGGGGAATTTCCCA.A.AGG.AAAGAGGGGGGAAGGGCCCCCTTCCCCGTCCGGGGCGGGGGCGCCGGGGAAGGGGCt                      >  1:4859381/1‑101 (MQ=255)


                                                                        cACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAgggccct                     >  2:2584054/1‑97 (MQ=255)


                                                                         cccTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTg                    <  2:221372/100‑1 (MQ=255)


                                                                         cccTGGGGCCAAATATCCC..GGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTgg                   <  1:4936346/100‑1 (MQ=255)


                                                                         atcTTGGGCCAAATACCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTg                    <  1:1926849/99‑1 (MQ=255)


                                                                          ccTTGGGGCAAAAAGCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTgg                   <  1:3721222/101‑1 (MQ=255)


                                                                           ctggggCGAAAGATCCG.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGc                  <  1:1953426/98‑1 (MQ=255)


                                                                           cTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGGGGCGCGCGGCAGGGGCTTGGc                  >  1:2980592/1‑101 (MQ=255)


                                                                             tGGGCCAAATATCCC.GGGGGGGGGGAACTCCCCG.A.AGG.AAAGGGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGCGCCACCCGGCAGGGGCTTGGCCg                >  1:3372914/1‑101 (MQ=255)


                                                                             tGGGCAAAATGTCCG.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCg                <  1:183286/101‑1 (MQ=255)


                                                                                  cAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGGCAg           >  1:1970365/1‑101 (MQ=255)


                                                                                  cAAATATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGGGCCGCGCGGCAGGGTCTTGGCCGGTCAg           >  1:539907/1‑101 (MQ=255)


                                                                                   aaaTATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGGCTTGGCCGGGCAgg          >  1:1351020/1‑101 (MQ=255)


                                                                                    tctacccc.GGGGGGGGGCAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAggc         <  2:494836/96‑1 (MQ=255)


                                                                                    aaTATCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAggc         >  1:4024364/1‑101 (MQ=255)


                                                                                    aaTATACC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAggc         >  2:1287985/1‑101 (MQ=255)


                                                                                       atCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAggcggg      >  2:587072/1‑100 (MQ=255)


                                                                                       atCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAggcggc      <  1:4612717/101‑1 (MQ=255)


                                                                                       atCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAggcggc      <  1:283367/101‑1 (MQ=255)


                                                                                       atCCC.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAggcaga      >  1:494901/1‑98 (MQ=255)


                                                                                         ccc.GGGGGGGGGGAACTTCCCG.N.AGG.NNNGNGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGgcgc    <  1:2796677/101‑1 (MQ=255)


                                                                                           c.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGgcgcgc  <  2:4726203/101‑1 (MQ=255)


                                                                                           c.GGGGGGGGGGAACTTCCCG.A.AGG.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGgcgcgc  >  1:1046152/1‑101 (MQ=255)


GCTGTGGTACCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGG.CTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCG.A.A.G.AAAGAGGGGGGCAGGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGGCGCGC  >  NC_014034/1255895‑1256084

Base quality scores:


ATCG

< 3 ≤


ATCG

< 14 ≤


ATCG

< 23 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG