Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,550 +C 8.0% intergenic (+362/‑569) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5501.→C 8.0% 81.2 ‑1.5 ‑1.6351intergenic (+362/‑569)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/19):  ref base (147/176):  total (156/195)
Fisher's exact test strand distribution p-value = 2.34e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.39e-02

Only 100 of 379 total aligned reads displayed.

GGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGG  >  NC_014034/1395450‑1395650
                                                                                                     |                                                                                                       
    gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                                     <  2:1526942/101‑1 (MQ=255)
    gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                                     >  2:5029150/1‑101 (MQ=255)
      ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGc                                                                                                   >  2:4170855/1‑101 (MQ=255)
        aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.Cg                                                                                                <  1:3654304/101‑1 (MQ=255)
          cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.c                                                                                             >  2:3641671/1‑101 (MQ=255)
             aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.g                                                                                         <  1:4768524/101‑1 (MQ=255)
               cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCt                                                                                       >  2:2980976/1‑101 (MQ=255)
               cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCt                                                                                       >  2:1498374/1‑101 (MQ=255)
                aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.CCCAGC.CGG.CCT.GCtc                                                                                      >  1:1076904/1‑101 (MQ=255)
                  cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCtcgt                                                                                    >  2:2645470/1‑101 (MQ=255)
                  cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCtcgt                                                                                    >  2:336874/1‑101 (MQ=255)
                      gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.ACT.GCTCTTCGcc                                                                                >  2:4214950/1‑100 (MQ=255)
                       cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTc                                                                               >  1:2256477/1‑101 (MQ=255)
                        tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTcc                                                                              >  2:3948998/1‑101 (MQ=255)
                         ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTccc                                                                             >  2:1795288/1‑101 (MQ=255)
                         ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCCCTccc                                                                             >  1:4972627/1‑101 (MQ=255)
                            gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTcccccc                                                                          <  2:2148512/101‑1 (MQ=255)
                            gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTcccccc                                                                          <  2:581116/101‑1 (MQ=255)
                              ggTCGGCGGGCTGGTAACGTGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCcgc                                                                        <  1:3934919/101‑1 (MQ=255)
                              ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCcgc                                                                        >  2:2762534/1‑101 (MQ=255)
                                   aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCggggg                                                                   >  1:3094561/1‑101 (MQ=255)
                                       gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTg                                                               <  1:4611117/101‑1 (MQ=255)
                                         tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGa                                                             <  2:2372520/101‑1 (MQ=255)
                                         gcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCACC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                                             <  2:1661797/92‑1 (MQ=0)
                                         gcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                                             <  2:1302225/92‑1 (MQ=0)
                                          ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            <  1:28874/93‑1 (MQ=2)
                                          ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            >  1:4833911/9‑101 (MQ=2)
                                          ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            >  1:2300646/1‑101 (MQ=255)
                                          cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            >  1:4396343/9‑101 (MQ=2)
                                          cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGGGGAt                                                            >  1:3464217/9‑101 (MQ=2)
                                           gccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  1:1988851/94‑1 (MQ=2)
                                           gTAACGTTTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                           >  2:2247891/1‑101 (MQ=255)
                                           gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  2:3891191/101‑1 (MQ=255)
                                           ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  1:1558154/94‑1 (MQ=2)
                                            gcggcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  2:3585850/95‑1 (MQ=2)
                                            ccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  1:3778561/95‑1 (MQ=2)
                                            ccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  1:4446630/95‑1 (MQ=2)
                                             ctgcggtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         <  1:1754332/96‑1 (MQ=2)
                                             cggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         <  1:490431/96‑1 (MQ=2)
                                             accGTGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         <  2:2435406/99‑1 (MQ=255)
                                              ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGGGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                        >  2:1945034/5‑101 (MQ=2)
                                               cgcgtCGCCCCCCCCGGGGGGGCGTGGATCGAAACACCCCGAACCTGTCG.GCG.GCGAACACCG.ATA.CCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       <  1:2752067/98‑1 (MQ=2)
                                                tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                      <  1:3168975/99‑1 (MQ=2)
                                                gtgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGGGGGTCGAAac                                                      >  1:895680/1‑101 (MQ=255)
                                                cggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                      <  2:3173527/99‑1 (MQ=2)
                                                 ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                     <  1:3701546/100‑1 (MQ=2)
                                                 cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAGCAATGCGGCGCGGGC.CTAT.GGCTGC.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                     <  1:1650046/100‑2 (MQ=2)
                                                  gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGC.CGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                     <  2:3020265/101‑2 (MQ=2)
                                                  gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactg                                                    >  1:4009300/1‑99 (MQ=2)
                                                   tCGCTCCGCCCGCGGGGGCGTGGATCGAAAC.TCCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                                   <  2:4508980/101‑4 (MQ=2)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                                   <  1:5053765/101‑4 (MQ=2)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                                   <  1:4543318/101‑3 (MQ=2)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                                  >  2:2544048/1‑97 (MQ=2)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgc                                                 >  1:2141749/1‑96 (MQ=2)
                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCACCtt                                                >  1:1927380/1‑101 (MQ=255)
                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccccg                                                >  2:908102/1‑99 (MQ=2)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                               >  1:1176760/1‑94 (MQ=2)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                               <  1:2438192/101‑8 (MQ=2)
                                                        ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcg                                              <  1:1300738/101‑9 (MQ=2)
                                                        ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                              >  1:780972/1‑94 (MQ=2)
                                                        ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                              <  2:4805210/101‑9 (MQ=2)
                                                         cccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCg                                             <  1:2983954/101‑1 (MQ=255)
                                                         cccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCg                                             >  2:1718024/1‑101 (MQ=255)
                                                         cccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgc                                             >  1:2126314/1‑92 (MQ=2)
                                                         cccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcg                                             >  2:3079507/1‑93 (MQ=0)
                                                          ccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCgg                                            <  1:4061078/101‑1 (MQ=255)
                                                          ccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGA.CGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgcg                                            <  2:2661674/101‑11 (MQ=2)
                                                          ccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAAC.CGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcgt                                            >  1:2193081/1‑92 (MQ=2)
                                                          ccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggct                                            >  1:3863349/1‑91 (MQ=2)
                                                          ccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTC.TGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggct                                            <  2:1590595/101‑11 (MQ=2)
                                                             cgcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                         <  1:336927/101‑1 (MQ=255)
                                                               tgggggCGCGAATCAAAACATCTCCGAACCGTCCCACG.TCCAGC.CGG.CCT.ACTCGTCGCACCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                                       <  2:2859752/100‑1 (MQ=25)
                                                               cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                                       <  2:1091778/101‑1 (MQ=255)
                                                                gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCCCTGCGGATTGtg                                      >  2:2080098/1‑100 (MQ=255)
                                                                 ggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                                     <  2:4410284/101‑1 (MQ=255)
                                                                    gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                                  <  1:980981/101‑1 (MQ=255)
                                                                     cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTTTCACttt                                 >  2:4627674/1‑101 (MQ=255)
                                                                     cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                                 <  1:1018856/101‑1 (MQ=255)
                                                                     cGTGGATCAAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                                 <  1:1507009/101‑1 (MQ=255)
                                                                      gTGGATCGAAACATCTCGGTGCCGTCGCTCN.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                <  1:602455/101‑1 (MQ=255)
                                                                      gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                <  1:1693341/101‑1 (MQ=255)
                                                                      gTGGAGCGAAACATCTCGGTCCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                <  2:3834238/101‑1 (MQ=255)
                                                                       tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                               >  2:3419674/1‑101 (MQ=255)
                                                                       tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTAACCTTcg                               >  2:220855/1‑101 (MQ=255)
                                                                          aTCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgg                            >  1:2587850/1‑101 (MQ=255)
                                                                             gAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCAAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                         >  1:4332161/1‑101 (MQ=255)
                                                                                 cATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                     <  1:640608/101‑1 (MQ=255)
                                                                                  aTCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                    <  2:4972297/101‑1 (MQ=255)
                                                                                  aTCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                    <  1:4267675/101‑1 (MQ=255)
                                                                                   tctcGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGGGAAGCg                   >  1:4566164/1‑101 (MQ=255)
                                                                                     tcGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGGGTc                 >  2:323419/1‑101 (MQ=255)
                                                                                     tcGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTc                 <  1:3305611/101‑1 (MQ=255)
                                                                                     tcGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTc                 <  1:183163/101‑1 (MQ=255)
                                                                                      cGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg                >  2:444413/1‑101 (MQ=255)
                                                                                      cGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg                <  1:4176050/101‑1 (MQ=255)
                                                                                          gCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcc            <  1:1533391/101‑1 (MQ=255)
                                                                                            cGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccc          >  1:352900/1‑101 (MQ=255)
                                                                                             gTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccccc         >  2:3762553/1‑101 (MQ=255)
                                                                                               cGCTCG.CCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcg       <  2:1832669/101‑1 (MQ=255)
                                                                                                    g.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGGAGCGTCCCTCCCCCCGCgggg  >  2:726036/1‑101 (MQ=255)
                                                                                                     |                                                                                                       
GGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGC.CGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGG  >  NC_014034/1395450‑1395650

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG