Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,028 +G 14.1% intergenic (+840/‑91) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0281.→G14.1% 131.0 ‑0.7 0.0290intergenic (+840/‑91)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/25):  ref base (114/135):  total (130/160)
Fisher's exact test strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

ACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395929‑1396128
                                                                                                    |                                                                                                      
aCGATGGGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGc.c                                                                                                       <  1:719728/101‑1 (MQ=255)
 cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.cg                                                                                                      <  1:4381923/101‑1 (MQ=255)
  gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.cgc                                                                                                     <  1:4312263/101‑1 (MQ=255)
   aTGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.cgc..g                                                                                                  >  2:523347/1‑101 (MQ=255)
       gTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTTC.CGC..GTCGc                                                                                              >  2:2833145/1‑101 (MQ=255)
       gTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGc                                                                                              <  1:4495840/101‑1 (MQ=255)
         tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTc                                                                                            >  2:4467914/1‑101 (MQ=255)
          cATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTcc                                                                                           >  1:475488/1‑101 (MQ=255)
          cATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTcc                                                                                           >  1:1375475/1‑101 (MQ=255)
           aTGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTccc                                                                                          >  1:2755161/1‑101 (MQ=255)
            tGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTcccc                                                                                         >  1:1492346/1‑101 (MQ=255)
            tGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTcccc                                                                                         >  1:3404837/1‑101 (MQ=255)
              gCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTcccccc                                                                                       >  1:2371862/1‑101 (MQ=255)
                cTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCcgc                                                                                     <  1:974746/101‑1 (MQ=255)
                 tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCcgcg                                                                                    >  1:661131/1‑101 (MQ=255)
                   cGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCggg                                                                                  >  2:3292698/1‑101 (MQ=255)
                    gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCgggg                                                                                 >  2:4363284/1‑101 (MQ=255)
                      cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                                               >  2:3828170/1‑101 (MQ=255)
                       gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCg                                                                              >  2:388777/1‑101 (MQ=255)
                       gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCg                                                                              >  1:1197335/1‑101 (MQ=255)
                          cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGGgg                                                                           >  1:2957750/1‑101 (MQ=255)
                           attgcacgggTCGCTCCCCCCGCGGGGGCGTGGATTGAAACTCTCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTTGCTCCCCCCGCGGGGGCGTGGa                                                                          <  2:769882/93‑1 (MQ=37)
                           aGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTggg                                                                          >  1:3026338/1‑100 (MQ=255)
                            tctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                          <  2:3547280/94‑1 (MQ=2)
                             tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  2:3671012/101‑1 (MQ=255)
                             tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        >  1:3096508/1‑101 (MQ=255)
                             tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  1:1566101/101‑1 (MQ=255)
                             tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  1:3501152/101‑1 (MQ=255)
                             ctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                         <  1:1861376/95‑1 (MQ=9)
                              tgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  1:3169869/96‑1 (MQ=9)
                              tgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  1:2058501/96‑1 (MQ=9)
                              tgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  2:4667301/96‑1 (MQ=9)
                              tgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                        <  2:2176019/96‑1 (MQ=9)
                               gcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTAAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                       >  2:2752502/5‑101 (MQ=9)
                                gcTGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     <  1:3858152/100‑1 (MQ=255)
                                aCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     >  1:538718/1‑101 (MQ=255)
                                   ggTCCCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                  <  2:895575/101‑1 (MQ=255)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                  >  2:495815/1‑100 (MQ=2)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                                 >  2:179940/1‑101 (MQ=255)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                                               >  1:2156435/1‑101 (MQ=255)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                                               >  1:3319480/1‑101 (MQ=255)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtga                                                               <  2:4002224/101‑5 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcg                                                              <  2:1467084/101‑1 (MQ=255)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgat                                                              <  2:1252050/101‑6 (MQ=2)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                                             <  2:4492004/101‑1 (MQ=255)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                                             >  2:1130162/1‑101 (MQ=255)
                                          ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCac                                                           >  2:1297279/1‑100 (MQ=255)
                                          ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                           >  1:2798659/1‑101 (MQ=255)
                                          ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                           >  1:3942142/1‑101 (MQ=255)
                                           cccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgac                                                           <  1:4624231/101‑9 (MQ=2)
                                            ccccGCGGGGGCTGGGATCGAAACTCGCCCTCGCTCCGCGCTTTCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCt                                                         <  1:2286803/101‑1 (MQ=38)
                                            ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                                          <  1:4878672/101‑10 (MQ=2)
                                            ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                                          >  1:617953/1‑92 (MQ=2)
                                            ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                                          <  1:3656896/101‑10 (MQ=2)
                                             cccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTg                                                        <  2:1350545/101‑1 (MQ=255)
                                                  gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATgga                                                   >  2:1699548/1‑99 (MQ=255)
                                                  gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                                   <  2:3026880/101‑1 (MQ=255)
                                                  gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                                   <  2:1060102/101‑1 (MQ=255)
                                                   ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                                  <  1:4420703/101‑1 (MQ=255)
                                                   ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                                  >  1:3623863/1‑101 (MQ=255)
                                                   ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                                  <  2:1180460/101‑1 (MQ=255)
                                                    gggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                                                 <  1:4552867/101‑1 (MQ=255)
                                                      gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                                               <  2:3941850/101‑1 (MQ=255)
                                                       cGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGc                                              >  1:2442402/1‑101 (MQ=255)
                                                         tGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                                            >  2:2545446/1‑101 (MQ=255)
                                                         tGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                                            <  2:3130025/101‑1 (MQ=255)
                                                          ggATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCg                                           >  2:1765770/1‑101 (MQ=255)
                                                          ggATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCg                                           >  2:7052/1‑101 (MQ=255)
                                                           gATCGAATCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCgg                                          <  2:4152976/101‑1 (MQ=255)
                                                            aTCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGt                                         <  1:1107337/101‑1 (MQ=255)
                                                             tCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg                                        <  1:2679661/101‑1 (MQ=255)
                                                             tCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg                                        <  2:2705881/101‑1 (MQ=255)
                                                             tCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg                                        <  1:2218191/101‑1 (MQ=255)
                                                             tCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg                                        <  2:2424970/101‑1 (MQ=255)
                                                              cGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACAGGCGCATCTGATGCATATGCCCGGTga                                       >  2:4423665/1‑101 (MQ=255)
                                                              cGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatatagatcggaa                                       >  1:3224743/1‑92 (MQ=255)
                                                               gAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                                      <  1:1765523/101‑1 (MQ=255)
                                                               gAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                                      <  2:1620685/101‑1 (MQ=255)
                                                               gAAACGCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                                      <  2:2051547/101‑1 (MQ=255)
                                                                aaccTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                                     <  2:3413377/98‑1 (MQ=255)
                                                                aaaCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                                     >  1:1043369/1‑101 (MQ=255)
                                                                aaaCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                                     <  2:2618568/101‑1 (MQ=255)
                                                                aaaCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                                     <  2:2290672/101‑1 (MQ=255)
                                                                 aaCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                                    <  1:1940407/101‑1 (MQ=255)
                                                                  aCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg                                   >  2:4299666/1‑101 (MQ=255)
                                                                  aCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg                                   <  1:2594396/101‑1 (MQ=255)
                                                                     cGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt                                >  1:1218898/1‑101 (MQ=255)
                                                                          tCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGc                           <  2:1973105/101‑1 (MQ=255)
                                                                           cGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCt                          >  1:1637116/1‑101 (MQ=255)
                                                                               ccGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc                      <  1:4205872/101‑1 (MQ=255)
                                                                                cgcgCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTccccc                     <  2:4872164/101‑1 (MQ=255)
                                                                                 gcgcTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc                    >  2:556899/1‑101 (MQ=255)
                                                                                         cTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc            <  1:816261/101‑1 (MQ=255)
                                                                                         cTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc            <  2:73282/101‑1 (MQ=255)
                                                                                           cACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCgg          >  2:1379114/1‑100 (MQ=255)
                                                                                             cGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTgg        <  2:475423/101‑1 (MQ=255)
                                                                                              gCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGa       <  1:1786005/101‑1 (MQ=255)
                                                                                               ccTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt      <  2:1043259/101‑1 (MQ=255)
                                                                                                cTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATc     >  2:822995/1‑101 (MQ=255)
                                                                                                 tGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCg    >  1:4973561/1‑101 (MQ=255)
                                                                                                    |                                                                                                      
ACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395929‑1396128

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG